Activator-independent gene expression in Neurospora crassa
A transgenic position effect that causes activator-independent gene expression has been described previously for three Neurospora crassa phosphate-repressible genes. We report analogous findings for two additional positively regulated genes, qa-2+ and ars-1+, indicating that such position effects ar...
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| Veröffentlicht in: | Genetics (Austin) 1996-02, Vol.142 (2), p.417-423 |
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| creator | Versaw, W.K. (University of Wisconsin, Madison, WI.) Metzenberg, R.L |
| description | A transgenic position effect that causes activator-independent gene expression has been described previously for three Neurospora crassa phosphate-repressible genes. We report analogous findings for two additional positively regulated genes, qa-2+ and ars-1+, indicating that such position effects are not limited to genes involved in phosphorus metabolism. In addition, we have characterized a number of mutants that display activator-independent gene expression. Each of these mutants contains a chromosomal rearrangement with one breakpoint located in the 5'-upstream region of the affected gene. This suggests that the rearrangements are associated with activator-independent gene expression and that these cis-acting mutations may represent a position effect similar to that responsible for rendering some transgenes independent of their transcriptional activators. We suggest that positively regulated genes in N. crassa are normally held in a transcriptionally repressed state by a cis-acting mechanism until specifically activated. Disruption of this cis-acting mechanism, either by random integration of a gene by transformation or by chromosomal rearrangement, renders these genes independent or partly independent of the transcriptional activator on which they normally depend |
| doi_str_mv | 10.1093/genetics/142.2.417 |
| format | Article |
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This suggests that the rearrangements are associated with activator-independent gene expression and that these cis-acting mutations may represent a position effect similar to that responsible for rendering some transgenes independent of their transcriptional activators. We suggest that positively regulated genes in N. crassa are normally held in a transcriptionally repressed state by a cis-acting mechanism until specifically activated. 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(University of Wisconsin, Madison, WI.)</creatorcontrib><creatorcontrib>Metzenberg, R.L</creatorcontrib><title>Activator-independent gene expression in Neurospora crassa</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>A transgenic position effect that causes activator-independent gene expression has been described previously for three Neurospora crassa phosphate-repressible genes. We report analogous findings for two additional positively regulated genes, qa-2+ and ars-1+, indicating that such position effects are not limited to genes involved in phosphorus metabolism. In addition, we have characterized a number of mutants that display activator-independent gene expression. Each of these mutants contains a chromosomal rearrangement with one breakpoint located in the 5'-upstream region of the affected gene. This suggests that the rearrangements are associated with activator-independent gene expression and that these cis-acting mutations may represent a position effect similar to that responsible for rendering some transgenes independent of their transcriptional activators. We suggest that positively regulated genes in N. crassa are normally held in a transcriptionally repressed state by a cis-acting mechanism until specifically activated. 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(University of Wisconsin, Madison, WI.)</creatorcontrib><creatorcontrib>Metzenberg, R.L</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Versaw, W.K. (University of Wisconsin, Madison, WI.)</au><au>Metzenberg, R.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activator-independent gene expression in Neurospora crassa</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1996-02</date><risdate>1996</risdate><volume>142</volume><issue>2</issue><spage>417</spage><epage>423</epage><pages>417-423</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>A transgenic position effect that causes activator-independent gene expression has been described previously for three Neurospora crassa phosphate-repressible genes. We report analogous findings for two additional positively regulated genes, qa-2+ and ars-1+, indicating that such position effects are not limited to genes involved in phosphorus metabolism. In addition, we have characterized a number of mutants that display activator-independent gene expression. Each of these mutants contains a chromosomal rearrangement with one breakpoint located in the 5'-upstream region of the affected gene. This suggests that the rearrangements are associated with activator-independent gene expression and that these cis-acting mutations may represent a position effect similar to that responsible for rendering some transgenes independent of their transcriptional activators. We suggest that positively regulated genes in N. crassa are normally held in a transcriptionally repressed state by a cis-acting mechanism until specifically activated. Disruption of this cis-acting mechanism, either by random integration of a gene by transformation or by chromosomal rearrangement, renders these genes independent or partly independent of the transcriptional activator on which they normally depend</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>8852841</pmid><doi>10.1093/genetics/142.2.417</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0016-6731 |
| ispartof | Genetics (Austin), 1996-02, Vol.142 (2), p.417-423 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | ADN Biochemistry Chromosomes, Fungal EXPRESION GENICA EXPRESSION DES GENES Gene Expression Regulation, Fungal Gene Rearrangement GENETICA Genetics GENETIQUE Investigations MUTACION MUTANT MUTANTES MUTATION NEUROSPORA CRASSA Neurospora crassa - genetics PROTEINAS AGLUTINANTES PROTEINE DE LIAISON TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transgenes |
| title | Activator-independent gene expression in Neurospora crassa |
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