White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development
The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These...
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Veröffentlicht in: | Genetics (Austin) 1995-11, Vol.141 (3), p.1075-1086 |
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creator | Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.) Tsai, C.J Green, M.M Chao, J.L Yu, C.T Jaw, T.J Yeh, J.Y Bolshakov, V.N |
description | The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These represent 12 distinct pigmentation patterns and probably 21 loci. The spatial pigmentation pattern is due to cis-acting suppression of white+ expression, and the suppression probably depends on cell position rather than cell type. The mechanism of suppression differs from inactivation by heterochromatin. In addition, activation of lacZ in P[lacW] occurs also in specific patterns in imaginal discs and embryos in many of the lines. The expression patterns of white+ and lacZ may reflect the activity of regulatory elements belonging to an endogenous gene near each P[lacW] insertion site. We speculate that these putative POSE (position-specific expression) genes may have a role in pattern formation of the eye as well as other imaginal structures. Three of the loci identified are optomotor-blind, engrailed and invected. teashirt is also implicated as a candidate gene. We propose that this "silencer trap" may be an efficient way of identifying genes involved in imaginal pattern formation |
doi_str_mv | 10.1093/genetics/141.3.1075 |
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(National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.) ; Tsai, C.J ; Green, M.M ; Chao, J.L ; Yu, C.T ; Jaw, T.J ; Yeh, J.Y ; Bolshakov, V.N</creator><creatorcontrib>Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.) ; Tsai, C.J ; Green, M.M ; Chao, J.L ; Yu, C.T ; Jaw, T.J ; Yeh, J.Y ; Bolshakov, V.N</creatorcontrib><description>The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These represent 12 distinct pigmentation patterns and probably 21 loci. The spatial pigmentation pattern is due to cis-acting suppression of white+ expression, and the suppression probably depends on cell position rather than cell type. The mechanism of suppression differs from inactivation by heterochromatin. In addition, activation of lacZ in P[lacW] occurs also in specific patterns in imaginal discs and embryos in many of the lines. The expression patterns of white+ and lacZ may reflect the activity of regulatory elements belonging to an endogenous gene near each P[lacW] insertion site. We speculate that these putative POSE (position-specific expression) genes may have a role in pattern formation of the eye as well as other imaginal structures. Three of the loci identified are optomotor-blind, engrailed and invected. teashirt is also implicated as a candidate gene. We propose that this "silencer trap" may be an efficient way of identifying genes involved in imaginal pattern formation</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/141.3.1075</identifier><identifier>PMID: 8582614</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>AILE ; ALAS ; Animals ; ATP-Binding Cassette Transporters ; BETA GALACTOSIDASA ; BETA GALACTOSIDASE ; DROSOPHILA MELANOGASTER ; Drosophila melanogaster - genetics ; Drosophila melanogaster - growth & development ; Drosophila Proteins ; EXPRESION GENICA ; EXPRESSION DES GENES ; Eye - growth & development ; Eye Color - genetics ; Eye Proteins ; GENE ; Gene Expression Regulation, Developmental ; GENES ; Genes, Reporter ; Genetic Complementation Test ; GENETICA ; GENETIQUE ; Insect Hormones - biosynthesis ; Insect Hormones - genetics ; Investigations ; LOCI ; LOCUS ; OEIL ; OJOS ; PIGMENTACION ; PIGMENTATION ; Regulatory Sequences, Nucleic Acid ; TRANSCRIPCION ; TRANSCRIPTION ; Transcription, Genetic - genetics</subject><ispartof>Genetics (Austin), 1995-11, Vol.141 (3), p.1075-1086</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-6302b62e36b083d2d5a916de61f352bf27125d1f08a24c3389d4f048f93d79b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,778,782,883,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8582614$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.)</creatorcontrib><creatorcontrib>Tsai, C.J</creatorcontrib><creatorcontrib>Green, M.M</creatorcontrib><creatorcontrib>Chao, J.L</creatorcontrib><creatorcontrib>Yu, C.T</creatorcontrib><creatorcontrib>Jaw, T.J</creatorcontrib><creatorcontrib>Yeh, J.Y</creatorcontrib><creatorcontrib>Bolshakov, V.N</creatorcontrib><title>White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These represent 12 distinct pigmentation patterns and probably 21 loci. The spatial pigmentation pattern is due to cis-acting suppression of white+ expression, and the suppression probably depends on cell position rather than cell type. The mechanism of suppression differs from inactivation by heterochromatin. In addition, activation of lacZ in P[lacW] occurs also in specific patterns in imaginal discs and embryos in many of the lines. The expression patterns of white+ and lacZ may reflect the activity of regulatory elements belonging to an endogenous gene near each P[lacW] insertion site. We speculate that these putative POSE (position-specific expression) genes may have a role in pattern formation of the eye as well as other imaginal structures. Three of the loci identified are optomotor-blind, engrailed and invected. teashirt is also implicated as a candidate gene. We propose that this "silencer trap" may be an efficient way of identifying genes involved in imaginal pattern formation</description><subject>AILE</subject><subject>ALAS</subject><subject>Animals</subject><subject>ATP-Binding Cassette Transporters</subject><subject>BETA GALACTOSIDASA</subject><subject>BETA GALACTOSIDASE</subject><subject>DROSOPHILA MELANOGASTER</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - growth & development</subject><subject>Drosophila Proteins</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Eye - growth & development</subject><subject>Eye Color - genetics</subject><subject>Eye Proteins</subject><subject>GENE</subject><subject>Gene Expression Regulation, Developmental</subject><subject>GENES</subject><subject>Genes, Reporter</subject><subject>Genetic Complementation Test</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>Insect Hormones - biosynthesis</subject><subject>Insect Hormones - genetics</subject><subject>Investigations</subject><subject>LOCI</subject><subject>LOCUS</subject><subject>OEIL</subject><subject>OJOS</subject><subject>PIGMENTACION</subject><subject>PIGMENTATION</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>TRANSCRIPCION</subject><subject>TRANSCRIPTION</subject><subject>Transcription, Genetic - genetics</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQhSMEKpfCC4CQvIJVWo-dOPEGCZVfqRILilhajjNJjJI42L693DfhcXGUS0VXrCzN-eaMZ06WvQB6AVTyyx5njNaESyjggqdaVT7IdiALnjPB4WG2oxRELioOj7MnIfyglApZ1mfZWV3WTECxy35_H2xEogPRxOPifERPVmMSHWkxookkej0H4-0SrZv1SIIdcTboAwkLGtsd7dyTxQW76vlWs2ZzwV-LxxCSQNq9X8F33gW3DHbUZMJRz67XYR2KR0wDb3F0y4RzfJo96vQY8NnpPc9uPry_ufqUX3_5-Pnq7XVuiprFXHDKGsGQi4bWvGVtqSWIFgV0vGRNxypgZQsdrTUrDOe1bIuOFnUneVvJhp9nbzbbZd9M2Jo02etRLd5O2h-V01bdV2Y7qN7dKmBU1BySwauTgXc_9xiimmwwOKbN0O2DqqqqrhiI_4IgpAQJRQL5Bpp0qeCxu_sNULUGr_4Gr1Lwiqs1-NT18t9F7npOSSf99aYPth8O1qMKkx7HRIM6HA73nJ5vZKed0r23QX37KtMdC17wP7p-xww</recordid><startdate>19951101</startdate><enddate>19951101</enddate><creator>Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.)</creator><creator>Tsai, C.J</creator><creator>Green, M.M</creator><creator>Chao, J.L</creator><creator>Yu, C.T</creator><creator>Jaw, T.J</creator><creator>Yeh, J.Y</creator><creator>Bolshakov, V.N</creator><general>Genetics Soc America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19951101</creationdate><title>White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development</title><author>Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.) ; Tsai, C.J ; Green, M.M ; Chao, J.L ; Yu, C.T ; Jaw, T.J ; Yeh, J.Y ; Bolshakov, V.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-6302b62e36b083d2d5a916de61f352bf27125d1f08a24c3389d4f048f93d79b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AILE</topic><topic>ALAS</topic><topic>Animals</topic><topic>ATP-Binding Cassette Transporters</topic><topic>BETA GALACTOSIDASA</topic><topic>BETA GALACTOSIDASE</topic><topic>DROSOPHILA MELANOGASTER</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - growth & development</topic><topic>Drosophila Proteins</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Eye - growth & development</topic><topic>Eye Color - genetics</topic><topic>Eye Proteins</topic><topic>GENE</topic><topic>Gene Expression Regulation, Developmental</topic><topic>GENES</topic><topic>Genes, Reporter</topic><topic>Genetic Complementation Test</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>Insect Hormones - biosynthesis</topic><topic>Insect Hormones - genetics</topic><topic>Investigations</topic><topic>LOCI</topic><topic>LOCUS</topic><topic>OEIL</topic><topic>OJOS</topic><topic>PIGMENTACION</topic><topic>PIGMENTATION</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><topic>Transcription, Genetic - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.)</creatorcontrib><creatorcontrib>Tsai, C.J</creatorcontrib><creatorcontrib>Green, M.M</creatorcontrib><creatorcontrib>Chao, J.L</creatorcontrib><creatorcontrib>Yu, C.T</creatorcontrib><creatorcontrib>Jaw, T.J</creatorcontrib><creatorcontrib>Yeh, J.Y</creatorcontrib><creatorcontrib>Bolshakov, V.N</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.)</au><au>Tsai, C.J</au><au>Green, M.M</au><au>Chao, J.L</au><au>Yu, C.T</au><au>Jaw, T.J</au><au>Yeh, J.Y</au><au>Bolshakov, V.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1995-11-01</date><risdate>1995</risdate><volume>141</volume><issue>3</issue><spage>1075</spage><epage>1086</epage><pages>1075-1086</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><abstract>The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These represent 12 distinct pigmentation patterns and probably 21 loci. The spatial pigmentation pattern is due to cis-acting suppression of white+ expression, and the suppression probably depends on cell position rather than cell type. The mechanism of suppression differs from inactivation by heterochromatin. In addition, activation of lacZ in P[lacW] occurs also in specific patterns in imaginal discs and embryos in many of the lines. The expression patterns of white+ and lacZ may reflect the activity of regulatory elements belonging to an endogenous gene near each P[lacW] insertion site. We speculate that these putative POSE (position-specific expression) genes may have a role in pattern formation of the eye as well as other imaginal structures. Three of the loci identified are optomotor-blind, engrailed and invected. teashirt is also implicated as a candidate gene. We propose that this "silencer trap" may be an efficient way of identifying genes involved in imaginal pattern formation</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>8582614</pmid><doi>10.1093/genetics/141.3.1075</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | AILE ALAS Animals ATP-Binding Cassette Transporters BETA GALACTOSIDASA BETA GALACTOSIDASE DROSOPHILA MELANOGASTER Drosophila melanogaster - genetics Drosophila melanogaster - growth & development Drosophila Proteins EXPRESION GENICA EXPRESSION DES GENES Eye - growth & development Eye Color - genetics Eye Proteins GENE Gene Expression Regulation, Developmental GENES Genes, Reporter Genetic Complementation Test GENETICA GENETIQUE Insect Hormones - biosynthesis Insect Hormones - genetics Investigations LOCI LOCUS OEIL OJOS PIGMENTACION PIGMENTATION Regulatory Sequences, Nucleic Acid TRANSCRIPCION TRANSCRIPTION Transcription, Genetic - genetics |
title | White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development |
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