White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development

The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These...

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Veröffentlicht in:Genetics (Austin) 1995-11, Vol.141 (3), p.1075-1086
Hauptverfasser: Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.), Tsai, C.J, Green, M.M, Chao, J.L, Yu, C.T, Jaw, T.J, Yeh, J.Y, Bolshakov, V.N
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container_issue 3
container_start_page 1075
container_title Genetics (Austin)
container_volume 141
creator Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.)
Tsai, C.J
Green, M.M
Chao, J.L
Yu, C.T
Jaw, T.J
Yeh, J.Y
Bolshakov, V.N
description The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These represent 12 distinct pigmentation patterns and probably 21 loci. The spatial pigmentation pattern is due to cis-acting suppression of white+ expression, and the suppression probably depends on cell position rather than cell type. The mechanism of suppression differs from inactivation by heterochromatin. In addition, activation of lacZ in P[lacW] occurs also in specific patterns in imaginal discs and embryos in many of the lines. The expression patterns of white+ and lacZ may reflect the activity of regulatory elements belonging to an endogenous gene near each P[lacW] insertion site. We speculate that these putative POSE (position-specific expression) genes may have a role in pattern formation of the eye as well as other imaginal structures. Three of the loci identified are optomotor-blind, engrailed and invected. teashirt is also implicated as a candidate gene. We propose that this "silencer trap" may be an efficient way of identifying genes involved in imaginal pattern formation
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(National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.)</creatorcontrib><creatorcontrib>Tsai, C.J</creatorcontrib><creatorcontrib>Green, M.M</creatorcontrib><creatorcontrib>Chao, J.L</creatorcontrib><creatorcontrib>Yu, C.T</creatorcontrib><creatorcontrib>Jaw, T.J</creatorcontrib><creatorcontrib>Yeh, J.Y</creatorcontrib><creatorcontrib>Bolshakov, V.N</creatorcontrib><title>White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These represent 12 distinct pigmentation patterns and probably 21 loci. 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(National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.) ; Tsai, C.J ; Green, M.M ; Chao, J.L ; Yu, C.T ; Jaw, T.J ; Yeh, J.Y ; Bolshakov, V.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-6302b62e36b083d2d5a916de61f352bf27125d1f08a24c3389d4f048f93d79b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AILE</topic><topic>ALAS</topic><topic>Animals</topic><topic>ATP-Binding Cassette Transporters</topic><topic>BETA GALACTOSIDASA</topic><topic>BETA GALACTOSIDASE</topic><topic>DROSOPHILA MELANOGASTER</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - growth &amp; development</topic><topic>Drosophila Proteins</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Eye - growth &amp; development</topic><topic>Eye Color - genetics</topic><topic>Eye Proteins</topic><topic>GENE</topic><topic>Gene Expression Regulation, Developmental</topic><topic>GENES</topic><topic>Genes, Reporter</topic><topic>Genetic Complementation Test</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>Insect Hormones - biosynthesis</topic><topic>Insect Hormones - genetics</topic><topic>Investigations</topic><topic>LOCI</topic><topic>LOCUS</topic><topic>OEIL</topic><topic>OJOS</topic><topic>PIGMENTACION</topic><topic>PIGMENTATION</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><topic>Transcription, Genetic - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.)</creatorcontrib><creatorcontrib>Tsai, C.J</creatorcontrib><creatorcontrib>Green, M.M</creatorcontrib><creatorcontrib>Chao, J.L</creatorcontrib><creatorcontrib>Yu, C.T</creatorcontrib><creatorcontrib>Jaw, T.J</creatorcontrib><creatorcontrib>Yeh, J.Y</creatorcontrib><creatorcontrib>Bolshakov, V.N</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Y.H. (National Yang Ming University, Shipai Taipei, Taiwan, Republic of China.)</au><au>Tsai, C.J</au><au>Green, M.M</au><au>Chao, J.L</au><au>Yu, C.T</au><au>Jaw, T.J</au><au>Yeh, J.Y</au><au>Bolshakov, V.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1995-11-01</date><risdate>1995</risdate><volume>141</volume><issue>3</issue><spage>1075</spage><epage>1086</epage><pages>1075-1086</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><abstract>The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These represent 12 distinct pigmentation patterns and probably 21 loci. The spatial pigmentation pattern is due to cis-acting suppression of white+ expression, and the suppression probably depends on cell position rather than cell type. The mechanism of suppression differs from inactivation by heterochromatin. In addition, activation of lacZ in P[lacW] occurs also in specific patterns in imaginal discs and embryos in many of the lines. The expression patterns of white+ and lacZ may reflect the activity of regulatory elements belonging to an endogenous gene near each P[lacW] insertion site. We speculate that these putative POSE (position-specific expression) genes may have a role in pattern formation of the eye as well as other imaginal structures. Three of the loci identified are optomotor-blind, engrailed and invected. teashirt is also implicated as a candidate gene. We propose that this "silencer trap" may be an efficient way of identifying genes involved in imaginal pattern formation</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>8582614</pmid><doi>10.1093/genetics/141.3.1075</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record>
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ispartof Genetics (Austin), 1995-11, Vol.141 (3), p.1075-1086
issn 0016-6731
1943-2631
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects AILE
ALAS
Animals
ATP-Binding Cassette Transporters
BETA GALACTOSIDASA
BETA GALACTOSIDASE
DROSOPHILA MELANOGASTER
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Drosophila Proteins
EXPRESION GENICA
EXPRESSION DES GENES
Eye - growth & development
Eye Color - genetics
Eye Proteins
GENE
Gene Expression Regulation, Developmental
GENES
Genes, Reporter
Genetic Complementation Test
GENETICA
GENETIQUE
Insect Hormones - biosynthesis
Insect Hormones - genetics
Investigations
LOCI
LOCUS
OEIL
OJOS
PIGMENTACION
PIGMENTATION
Regulatory Sequences, Nucleic Acid
TRANSCRIPCION
TRANSCRIPTION
Transcription, Genetic - genetics
title White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development
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