A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus

A restriction fragment length polymorphism (RFLP) map has been constructed of the nuclear genome of the plant pathogenic ascomycete Cochliobolus heterostrophus. The segregation of 128 RFLP and 4 phenotypic markers was analyzed among 91 random progeny of a single cross; linkages were detected among 1...

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Veröffentlicht in:Genetics (Austin) 1992-01, Vol.130 (1), p.81-96
Hauptverfasser: Tzeng, T.H. (State University of New York, Buffalo, NY), Lyngholm, L.K, Ford, C.F, Bronson, C.R
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creator Tzeng, T.H. (State University of New York, Buffalo, NY)
Lyngholm, L.K
Ford, C.F
Bronson, C.R
description A restriction fragment length polymorphism (RFLP) map has been constructed of the nuclear genome of the plant pathogenic ascomycete Cochliobolus heterostrophus. The segregation of 128 RFLP and 4 phenotypic markers was analyzed among 91 random progeny of a single cross; linkages were detected among 126 of the markers. The intact chromosomal DNAs of the parents and certain progeny were separated using pulsed field gel electrophoresis and hybridized with probes used to detect the RFLPs. In this way, 125 markers were assigned to specific chromosomes and linkages among 120 of the markers were confirmed. These linkages totalled 941 centimorgans (cM). Several RFLPs and a reciprocal translocation were identified tightly linked to Tox1, a locus controlling host-specific virulence. Other differences in chromosome arrangement between the parents were also detected. Fourteen gaps of at least 40 cM were identified between linkage groups on the same chromosomes; the total map length was therefore estimated to be, at a minimum, 1501 cM. Fifteen A chromosomes ranging from about 1.3 megabases (Mb) to about 3.7 Mb were identified; one of the strains also has an apparent B chromosome. This chromosome appears to be completely dispensable; in some progeny, all of 15 markers that mapped to this chromosome were absent. The total genome size was estimated to be roughly 35 Mb. Based on these estimates of map length and physical genome size, the average kb/cM ratio in this cross was calculated to be approximately 23. This low ratio of physical length to map distance should make this RFLP map a useful tool for cloning genes
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(State University of New York, Buffalo, NY) ; Lyngholm, L.K ; Ford, C.F ; Bronson, C.R</creator><creatorcontrib>Tzeng, T.H. (State University of New York, Buffalo, NY) ; Lyngholm, L.K ; Ford, C.F ; Bronson, C.R</creatorcontrib><description>A restriction fragment length polymorphism (RFLP) map has been constructed of the nuclear genome of the plant pathogenic ascomycete Cochliobolus heterostrophus. The segregation of 128 RFLP and 4 phenotypic markers was analyzed among 91 random progeny of a single cross; linkages were detected among 126 of the markers. The intact chromosomal DNAs of the parents and certain progeny were separated using pulsed field gel electrophoresis and hybridized with probes used to detect the RFLPs. In this way, 125 markers were assigned to specific chromosomes and linkages among 120 of the markers were confirmed. These linkages totalled 941 centimorgans (cM). Several RFLPs and a reciprocal translocation were identified tightly linked to Tox1, a locus controlling host-specific virulence. Other differences in chromosome arrangement between the parents were also detected. Fourteen gaps of at least 40 cM were identified between linkage groups on the same chromosomes; the total map length was therefore estimated to be, at a minimum, 1501 cM. Fifteen A chromosomes ranging from about 1.3 megabases (Mb) to about 3.7 Mb were identified; one of the strains also has an apparent B chromosome. This chromosome appears to be completely dispensable; in some progeny, all of 15 markers that mapped to this chromosome were absent. The total genome size was estimated to be roughly 35 Mb. Based on these estimates of map length and physical genome size, the average kb/cM ratio in this cross was calculated to be approximately 23. 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(State University of New York, Buffalo, NY)</creatorcontrib><creatorcontrib>Lyngholm, L.K</creatorcontrib><creatorcontrib>Ford, C.F</creatorcontrib><creatorcontrib>Bronson, C.R</creatorcontrib><title>A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>A restriction fragment length polymorphism (RFLP) map has been constructed of the nuclear genome of the plant pathogenic ascomycete Cochliobolus heterostrophus. The segregation of 128 RFLP and 4 phenotypic markers was analyzed among 91 random progeny of a single cross; linkages were detected among 126 of the markers. The intact chromosomal DNAs of the parents and certain progeny were separated using pulsed field gel electrophoresis and hybridized with probes used to detect the RFLPs. In this way, 125 markers were assigned to specific chromosomes and linkages among 120 of the markers were confirmed. These linkages totalled 941 centimorgans (cM). Several RFLPs and a reciprocal translocation were identified tightly linked to Tox1, a locus controlling host-specific virulence. Other differences in chromosome arrangement between the parents were also detected. Fourteen gaps of at least 40 cM were identified between linkage groups on the same chromosomes; the total map length was therefore estimated to be, at a minimum, 1501 cM. Fifteen A chromosomes ranging from about 1.3 megabases (Mb) to about 3.7 Mb were identified; one of the strains also has an apparent B chromosome. This chromosome appears to be completely dispensable; in some progeny, all of 15 markers that mapped to this chromosome were absent. The total genome size was estimated to be roughly 35 Mb. 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Biological and molecular evolution</subject><subject>Genome, Fungal</subject><subject>Investigations</subject><subject>Karyotyping</subject><subject>LOCALISATION DE GENE</subject><subject>LOCALIZACION DE GENES</subject><subject>LOCI</subject><subject>LOCUS</subject><subject>MAPAS GENETICOS</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>Medical research</subject><subject>PODER PATOGENO</subject><subject>POLIMORFISMO</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>POLYMORPHISME</subject><subject>POUVOIR PATHOGENE</subject><subject>Restriction Mapping</subject><subject>SEGREGACION</subject><subject>SEGREGATION</subject><subject>Thallophyta, bryophyta</subject><subject>Translocation, Genetic - genetics</subject><subject>Vegetals</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU9v1DAQxSMEKtvCBwAJyUKFW1qPnTjOBala8U-qxAF6thzHSVycONgOq-XO98ZLllI4WfL85s28eVn2DPAF4Jpe9nrS0ahwCTT9XHB4kG2gLmhOGIWH2QZjYDmrKDzOTkO4xRizuuQn2QnQghEGm-znFfI6RG9UNG5CnZf9qKeIrJ76OKDZ2f3o_DyYMKJRzkhOLdJWq-jdPDh_mI6-Sr93cT9r5DoUB426ZeqlTbz5odEs4-DSomjr1GCNa5xdAhp01N6F3zJLeJI96qQN-unxPctu3r39sv2QX396_3F7dZ2rsmYxLzAjHQXglLRUSSV129CSK6qhUgyXktcdUxXvukZK4IpxxlpS1yVtWdOku5xlb1bdeWlG3ark1EsrZm_G5EE4acS_lckMonffBRBccMyTwOujgHfflnQ4MZqgtLVy0m4JAlhJClqRBL78D7x1i5-SOUGgAEJ4USYIVkilUwSvu7tNAItDwOJPwCIFLEBwSD0v7lv427Emmurnx7oMStoU6KRMuMNKzHldHbBXKzaYftgZr0UYpbVJFMRut7s37vnKddIJ2fskdfO5BkbrqqS_AOqyyoI</recordid><startdate>199201</startdate><enddate>199201</enddate><creator>Tzeng, T.H. (State University of New York, Buffalo, NY)</creator><creator>Lyngholm, L.K</creator><creator>Ford, C.F</creator><creator>Bronson, C.R</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>199201</creationdate><title>A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus</title><author>Tzeng, T.H. 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Psychology</topic><topic>Fungi</topic><topic>GENE</topic><topic>GENES</topic><topic>Genetic Linkage - genetics</topic><topic>Genetics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genome, Fungal</topic><topic>Investigations</topic><topic>Karyotyping</topic><topic>LOCALISATION DE GENE</topic><topic>LOCALIZACION DE GENES</topic><topic>LOCI</topic><topic>LOCUS</topic><topic>MAPAS GENETICOS</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>Medical research</topic><topic>PODER PATOGENO</topic><topic>POLIMORFISMO</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>POLYMORPHISME</topic><topic>POUVOIR PATHOGENE</topic><topic>Restriction Mapping</topic><topic>SEGREGACION</topic><topic>SEGREGATION</topic><topic>Thallophyta, bryophyta</topic><topic>Translocation, Genetic - genetics</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tzeng, T.H. 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(State University of New York, Buffalo, NY)</au><au>Lyngholm, L.K</au><au>Ford, C.F</au><au>Bronson, C.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1992-01</date><risdate>1992</risdate><volume>130</volume><issue>1</issue><spage>81</spage><epage>96</epage><pages>81-96</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>A restriction fragment length polymorphism (RFLP) map has been constructed of the nuclear genome of the plant pathogenic ascomycete Cochliobolus heterostrophus. The segregation of 128 RFLP and 4 phenotypic markers was analyzed among 91 random progeny of a single cross; linkages were detected among 126 of the markers. The intact chromosomal DNAs of the parents and certain progeny were separated using pulsed field gel electrophoresis and hybridized with probes used to detect the RFLPs. In this way, 125 markers were assigned to specific chromosomes and linkages among 120 of the markers were confirmed. These linkages totalled 941 centimorgans (cM). Several RFLPs and a reciprocal translocation were identified tightly linked to Tox1, a locus controlling host-specific virulence. Other differences in chromosome arrangement between the parents were also detected. Fourteen gaps of at least 40 cM were identified between linkage groups on the same chromosomes; the total map length was therefore estimated to be, at a minimum, 1501 cM. Fifteen A chromosomes ranging from about 1.3 megabases (Mb) to about 3.7 Mb were identified; one of the strains also has an apparent B chromosome. This chromosome appears to be completely dispensable; in some progeny, all of 15 markers that mapped to this chromosome were absent. The total genome size was estimated to be roughly 35 Mb. Based on these estimates of map length and physical genome size, the average kb/cM ratio in this cross was calculated to be approximately 23. This low ratio of physical length to map distance should make this RFLP map a useful tool for cloning genes</abstract><cop>Bethesda, MD</cop><pub>Genetics Soc America</pub><pmid>1346261</pmid><doi>10.1093/genetics/130.1.81</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record>
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ispartof Genetics (Austin), 1992-01, Vol.130 (1), p.81-96
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subjects Ascomycota - genetics
Biological and medical sciences
CARIOTIPOS
CARTE GENETIQUE
CARYOTYPE
CHROMOSOME
Classical genetics, quantitative genetics, hybrids
COCHLIOBOLUS
Cochliobolus heterostrophus
CROMOSOMAS
Crosses, Genetic
ELECTROFORESIS
ELECTROPHORESE
Electrophoresis
Fundamental and applied biological sciences. Psychology
Fungi
GENE
GENES
Genetic Linkage - genetics
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Genome, Fungal
Investigations
Karyotyping
LOCALISATION DE GENE
LOCALIZACION DE GENES
LOCI
LOCUS
MAPAS GENETICOS
MARCADORES GENETICOS
MARQUEUR GENETIQUE
Medical research
PODER PATOGENO
POLIMORFISMO
Polymorphism, Restriction Fragment Length
POLYMORPHISME
POUVOIR PATHOGENE
Restriction Mapping
SEGREGACION
SEGREGATION
Thallophyta, bryophyta
Translocation, Genetic - genetics
Vegetals
title A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus
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