Assessment of inbreeding by DNA fingerprinting: development of a calibration curve using defined strains of chickens
By analyzing DNA fingerprints of chickens from seven well-defined genetic groups, a calibration curve was established relating the degree of inbreeding with the average band frequency, allelic frequency and band sharing. The probe used was bacteriophage M13 DNA and digestion of the genomic DNA was c...
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| Veröffentlicht in: | Genetics (Austin) 1990-05, Vol.125 (1), p.161-165 |
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| container_title | Genetics (Austin) |
| container_volume | 125 |
| creator | Kuhnlein, U. (Macdonald College of McGill University, Ste. Anne de Bellevue, Quebec, Canada) Zadworny, D Dawe, Y Fairfull, R.W Gavora, J.S |
| description | By analyzing DNA fingerprints of chickens from seven well-defined genetic groups, a calibration curve was established relating the degree of inbreeding with the average band frequency, allelic frequency and band sharing. The probe used was bacteriophage M13 DNA and digestion of the genomic DNA was carried out with the MspI restriction enzyme. The analysis also provided an estimate of the average allelic frequency at a hypervariable locus and the average mutation frequency per locus and generation. The values of 0.24 and 1.7 x 10-3, respectively, are similar to the estimates for humans using other probes and hybridization protocols. It is suggested that the calibration curve established can be used for determining inbreeding not only in chickens, but also in other species |
| doi_str_mv | 10.1093/genetics/125.1.161 |
| format | Article |
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(Macdonald College of McGill University, Ste. Anne de Bellevue, Quebec, Canada)</creatorcontrib><creatorcontrib>Zadworny, D</creatorcontrib><creatorcontrib>Dawe, Y</creatorcontrib><creatorcontrib>Fairfull, R.W</creatorcontrib><creatorcontrib>Gavora, J.S</creatorcontrib><title>Assessment of inbreeding by DNA fingerprinting: development of a calibration curve using defined strains of chickens</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>By analyzing DNA fingerprints of chickens from seven well-defined genetic groups, a calibration curve was established relating the degree of inbreeding with the average band frequency, allelic frequency and band sharing. The probe used was bacteriophage M13 DNA and digestion of the genomic DNA was carried out with the MspI restriction enzyme. The analysis also provided an estimate of the average allelic frequency at a hypervariable locus and the average mutation frequency per locus and generation. The values of 0.24 and 1.7 x 10-3, respectively, are similar to the estimates for humans using other probes and hybridization protocols. It is suggested that the calibration curve established can be used for determining inbreeding not only in chickens, but also in other species</description><subject>ADN</subject><subject>Alleles</subject><subject>ANALISIS</subject><subject>ANALYSE</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Chickens - genetics</subject><subject>DNA - genetics</subject><subject>ENDOGAMIA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>INBREEDING</subject><subject>Invertebrata</subject><subject>Investigations</subject><subject>LIGNEE CONSANGUINE</subject><subject>LINEAS CONSANGUINEAS</subject><subject>LOCI</subject><subject>LOCUS</subject><subject>MUTACION</subject><subject>MUTATION</subject><subject>Nucleotide Mapping</subject><subject>POLLO</subject><subject>Population genetics, reproduction patterns</subject><subject>POULET</subject><subject>RACE</subject><subject>RAZAS</subject><subject>Reference Values</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU2P0zAQtRBoKQt_YCUkHxC3dP0ROzEHpGr5lFZwgD1bjj1ODalT7KTV_ntctVuW04w07715Mw-hK0qWlCh-3UOEKdh8TZlY0iWV9AlaUFXziklOn6IFIVRWsuH0OXqR8y9CiFSivUAXjNeUsHaBplXOkPMG4oRHj0PsEoALscfdPf7wbYV96SFtU4hT6d5hBzsYxu0DwWBrhtAlM4UxYjunHeA5H_gOChUczlMyIeYD2K6D_Q0xv0TPvBkyvDrVS3T36ePPmy_V7ffPX29Wt5UVNZuqYtUAc8zZthzoBTWEW9FYAMsbKoVXljTEW-686hqgwhgpwNUtd4LXNeOX6P1Rdzt3G3C2eE5m0OWYjUn3ejRB_z-JYa37cacpI1yptgi8PQmk8c8MedKbkC0Mg4kwzlk3qmmkpKQA2RFo05hzAn9eQok-ZKUfsiraQlNdsiqk14_tnSmncMr8zWlucvmyTybakM8w2SrC6uafyXXo1_uQQOeNGYYiSvV-v3-87-oI9GbUpk9F6-6HInV5Zcv_AvpCt3I</recordid><startdate>19900501</startdate><enddate>19900501</enddate><creator>Kuhnlein, U. (Macdonald College of McGill University, Ste. Anne de Bellevue, Quebec, Canada)</creator><creator>Zadworny, D</creator><creator>Dawe, Y</creator><creator>Fairfull, R.W</creator><creator>Gavora, J.S</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900501</creationdate><title>Assessment of inbreeding by DNA fingerprinting: development of a calibration curve using defined strains of chickens</title><author>Kuhnlein, U. (Macdonald College of McGill University, Ste. Anne de Bellevue, Quebec, Canada) ; Zadworny, D ; Dawe, Y ; Fairfull, R.W ; Gavora, J.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-958ae2d2dc8125f51a03c57ceec37165f9c070fc3df9b7e15aa65ed483d534423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>ADN</topic><topic>Alleles</topic><topic>ANALISIS</topic><topic>ANALYSE</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Chickens - genetics</topic><topic>DNA - genetics</topic><topic>ENDOGAMIA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genetics of eukaryotes. 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The analysis also provided an estimate of the average allelic frequency at a hypervariable locus and the average mutation frequency per locus and generation. The values of 0.24 and 1.7 x 10-3, respectively, are similar to the estimates for humans using other probes and hybridization protocols. It is suggested that the calibration curve established can be used for determining inbreeding not only in chickens, but also in other species</abstract><cop>Bethesda, MD</cop><pub>Genetics Soc America</pub><pmid>2341028</pmid><doi>10.1093/genetics/125.1.161</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genetics (Austin), 1990-05, Vol.125 (1), p.161-165 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1203998 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ADN Alleles ANALISIS ANALYSE Animals Biological and medical sciences Calibration Chickens - genetics DNA - genetics ENDOGAMIA Fundamental and applied biological sciences. Psychology GENE GENES Genetics of eukaryotes. Biological and molecular evolution INBREEDING Invertebrata Investigations LIGNEE CONSANGUINE LINEAS CONSANGUINEAS LOCI LOCUS MUTACION MUTATION Nucleotide Mapping POLLO Population genetics, reproduction patterns POULET RACE RAZAS Reference Values |
| title | Assessment of inbreeding by DNA fingerprinting: development of a calibration curve using defined strains of chickens |
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