Protease B of Saccharomyces cerevisiae: Isolation and Regulation of the PRB1 Structural Gene

We have isolated the structural gene, PRB1, for the vacuolar protease B of Saccharomyces cerevisiae from a genomic library by complementation of the prb1-1122 mutation. Deletion analysis localized the complementing activity to a 3.2-kilobase pair XhoI-HindIII restriction enzyme fragment. The fragmen...

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Veröffentlicht in:	Genetics (Austin) 1987-02, Vol.115 (2), p.255-263
Hauptverfasser:	Moehle, Charles M, Aynardi, Martha W, Kolodny, Michael R, Park, Frances J, Jones, Elizabeth W
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Endopeptidases - genetics Fundamental and applied biological sciences. Psychology Genes Genes, Fungal Genes. Genome Genetic Complementation Test Investigations Molecular and cellular biology Molecular genetics Mutation Nucleic Acid Hybridization RNA, Messenger - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Serine Endopeptidases Vacuoles - enzymology
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