DNA SEQUENCE ANALYSIS OF MUTAGENICITY AND SITE SPECIFICITY OF ETHYL METHANESULFONATE IN Uvr+ AND UvrB - STRAINS OF ESCHERICHIA COLI
EMS-induced mutations within a 180 base pair region of the lacI gene of E. coli were cloned and sequenced. In total, 105 and 79 EMS-induced mutations from a Uvr+ and a UvrB- strain, respectively, were sequenced. The specificity of EMS-induced mutagenesis was very similar in the two strains; G:C---A:...
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| Veröffentlicht in: | Genetics (Austin) 1986-08, Vol.113 (4), p.811-819 |
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| creator | Burns, Philip A Allen, Frances L Glickman, Barry W |
| description | EMS-induced mutations within a 180 base pair region of the lacI gene of E. coli were cloned and sequenced. In total, 105 and 79 EMS-induced mutations from a Uvr+ and a UvrB- strain, respectively, were sequenced. The specificity of EMS-induced mutagenesis was very similar in the two strains; G:C---A:T transitions accounted for all but three of the mutants. The overall frequency of induced mutation was fivefold higher in the UvrB- strain compared to the Uvr+ strain. This demonstrates, at the DNA sequence level, that the presumed premutagenic lesion, O6-ethylguanine, is subject to repair by the uvrABC excision repair system of E. coli. An analysis of mutation frequencies with respect to neighboring base sequence, in the two strains, shows that O6-ethylguanine lesions adjacent to A:T base pairs present better targets for the excision repair machinery than those not adjacent to A:T base pairs. |
| doi_str_mv | 10.1093/genetics/113.4.811 |
| format | Article |
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An analysis of mutation frequencies with respect to neighboring base sequence, in the two strains, shows that O6-ethylguanine lesions adjacent to A:T base pairs present better targets for the excision repair machinery than those not adjacent to A:T base pairs.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/113.4.811</identifier><identifier>PMID: 3527868</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>Base Sequence ; Cloning, Molecular ; DNA, Bacterial - genetics ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Escherichia coli - radiation effects ; Ethyl Methanesulfonate - pharmacology ; Genes, Bacterial - drug effects ; Investigations ; Mutation ; Species Specificity ; Ultraviolet Rays</subject><ispartof>Genetics (Austin), 1986-08, Vol.113 (4), p.811-819</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-8b907c08fa913753ffe4841813c75a20f5674922a1a57c4b605435205afd828c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3527868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burns, Philip A</creatorcontrib><creatorcontrib>Allen, Frances L</creatorcontrib><creatorcontrib>Glickman, Barry W</creatorcontrib><title>DNA SEQUENCE ANALYSIS OF MUTAGENICITY AND SITE SPECIFICITY OF ETHYL METHANESULFONATE IN Uvr+ AND UvrB - STRAINS OF ESCHERICHIA COLI</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>EMS-induced mutations within a 180 base pair region of the lacI gene of E. coli were cloned and sequenced. In total, 105 and 79 EMS-induced mutations from a Uvr+ and a UvrB- strain, respectively, were sequenced. The specificity of EMS-induced mutagenesis was very similar in the two strains; G:C---A:T transitions accounted for all but three of the mutants. The overall frequency of induced mutation was fivefold higher in the UvrB- strain compared to the Uvr+ strain. This demonstrates, at the DNA sequence level, that the presumed premutagenic lesion, O6-ethylguanine, is subject to repair by the uvrABC excision repair system of E. coli. An analysis of mutation frequencies with respect to neighboring base sequence, in the two strains, shows that O6-ethylguanine lesions adjacent to A:T base pairs present better targets for the excision repair machinery than those not adjacent to A:T base pairs.</description><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>DNA, Bacterial - genetics</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - radiation effects</subject><subject>Ethyl Methanesulfonate - pharmacology</subject><subject>Genes, Bacterial - drug effects</subject><subject>Investigations</subject><subject>Mutation</subject><subject>Species Specificity</subject><subject>Ultraviolet Rays</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtP4zAUhS00iCmPP4A0klezQSm-tpM4GyQTXGoppECSRVeWG5I2qA-UFCrW_HEM5bm6V_ec81nWQegYSB9IxE6n1bJaN2V3CsD6vC8AdlAPIs48GjD4g3qEQOAFIYO_aL_r7gkhQeSLPbTHfBqKQPTQy0UqcaZuCpXGCstUJuNMZ3g0wFdFLi9VqmOdj51wgTOdK5xdq1gPtkdnUvlwnOArN2SqsiIZjFLpXDrFxVN78h5zyzn2cJbfSp2-k1UWD9Wtjoda4niU6EO0W9t5Vx19zANUDFQeD71kdKljmXglj_jaE5OIhCURtY2AhT6r64oLDgJYGfqWktoPQh5RasH6YcknAfG5-yfxbX0nqCjZATrbch8eJ4vqrqyW69bOzUPbLGz7bFa2Mb-VZTMz09WTAUpoBNwB6BZQtquua6v6KwvEvDViPhsxrhHDjWvEhf79fPUr8lGB0_9v9VkznW2atjLdws7nzg1ms9l8g14B_7-MpA</recordid><startdate>19860801</startdate><enddate>19860801</enddate><creator>Burns, Philip A</creator><creator>Allen, Frances L</creator><creator>Glickman, Barry W</creator><general>Genetics Soc America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19860801</creationdate><title>DNA SEQUENCE ANALYSIS OF MUTAGENICITY AND SITE SPECIFICITY OF ETHYL METHANESULFONATE IN Uvr+ AND UvrB - STRAINS OF ESCHERICHIA COLI</title><author>Burns, Philip A ; Allen, Frances L ; Glickman, Barry W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-8b907c08fa913753ffe4841813c75a20f5674922a1a57c4b605435205afd828c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>DNA, Bacterial - genetics</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - radiation effects</topic><topic>Ethyl Methanesulfonate - pharmacology</topic><topic>Genes, Bacterial - drug effects</topic><topic>Investigations</topic><topic>Mutation</topic><topic>Species Specificity</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burns, Philip A</creatorcontrib><creatorcontrib>Allen, Frances L</creatorcontrib><creatorcontrib>Glickman, Barry W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burns, Philip A</au><au>Allen, Frances L</au><au>Glickman, Barry W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA SEQUENCE ANALYSIS OF MUTAGENICITY AND SITE SPECIFICITY OF ETHYL METHANESULFONATE IN Uvr+ AND UvrB - STRAINS OF ESCHERICHIA COLI</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1986-08-01</date><risdate>1986</risdate><volume>113</volume><issue>4</issue><spage>811</spage><epage>819</epage><pages>811-819</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><abstract>EMS-induced mutations within a 180 base pair region of the lacI gene of E. coli were cloned and sequenced. In total, 105 and 79 EMS-induced mutations from a Uvr+ and a UvrB- strain, respectively, were sequenced. The specificity of EMS-induced mutagenesis was very similar in the two strains; G:C---A:T transitions accounted for all but three of the mutants. The overall frequency of induced mutation was fivefold higher in the UvrB- strain compared to the Uvr+ strain. This demonstrates, at the DNA sequence level, that the presumed premutagenic lesion, O6-ethylguanine, is subject to repair by the uvrABC excision repair system of E. coli. An analysis of mutation frequencies with respect to neighboring base sequence, in the two strains, shows that O6-ethylguanine lesions adjacent to A:T base pairs present better targets for the excision repair machinery than those not adjacent to A:T base pairs.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>3527868</pmid><doi>10.1093/genetics/113.4.811</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0016-6731 |
| ispartof | Genetics (Austin), 1986-08, Vol.113 (4), p.811-819 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1202914 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Base Sequence Cloning, Molecular DNA, Bacterial - genetics Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - radiation effects Ethyl Methanesulfonate - pharmacology Genes, Bacterial - drug effects Investigations Mutation Species Specificity Ultraviolet Rays |
| title | DNA SEQUENCE ANALYSIS OF MUTAGENICITY AND SITE SPECIFICITY OF ETHYL METHANESULFONATE IN Uvr+ AND UvrB - STRAINS OF ESCHERICHIA COLI |
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