Sodium channels in cultured cardiac cells
Primary cardiac cell cultures were prepared from the hearts of neonatal rats. The patch-clamp method (Hamill, Marty, Neher, Sakmann & Sigworth, 1981) was applied for studying whole-cell Na+ currents and single-channel Na+ currents, respectively. Whole-cell recordings yielded voltage- and time-de...
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| Veröffentlicht in: | The Journal of physiology 1983-07, Vol.340 (1), p.389-401 |
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| creator | Cachelin, A B De Peyer, J E Kokubun, S Reuter, H |
| description | Primary cardiac cell cultures were prepared from the hearts of neonatal rats. The patch-clamp method (Hamill, Marty, Neher,
Sakmann & Sigworth, 1981) was applied for studying whole-cell Na+ currents and single-channel Na+ currents, respectively.
Whole-cell recordings yielded voltage- and time-dependent Na+ currents which could be blocked by tetrodotoxin. Single-channel
Na+ currents were directly compared in cell-attached patches and in inside-out patches. In cell-attached patches the elementary
current was about -1 pA at -10 mV and the slope conductance over a 50 mV voltage range was 15.1 +/- 1.6 pS (mean +/- S.D.).
Inactivation during depolarization and after conditioning clamp steps, in the steady state, resulted from a reduced opening
probability of Na+ channels. In inside-out patches, with identical solutions at both membrane surfaces, there was a large
(40-50 mV) shift of channel opening and inactivation kinetics towards more negative potentials. However, for levels of comparable
opening probabilities, mean open times of Na+ channels were similar in cell-attached and inside-out patches. Tetrodotoxin
(10-20 microM) had no effect on Na+ channels when applied from the inside, but blocked them completely after application to
the outside membrane surface. |
| doi_str_mv | 10.1113/jphysiol.1983.sp014768 |
| format | Article |
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Sakmann & Sigworth, 1981) was applied for studying whole-cell Na+ currents and single-channel Na+ currents, respectively.
Whole-cell recordings yielded voltage- and time-dependent Na+ currents which could be blocked by tetrodotoxin. Single-channel
Na+ currents were directly compared in cell-attached patches and in inside-out patches. In cell-attached patches the elementary
current was about -1 pA at -10 mV and the slope conductance over a 50 mV voltage range was 15.1 +/- 1.6 pS (mean +/- S.D.).
Inactivation during depolarization and after conditioning clamp steps, in the steady state, resulted from a reduced opening
probability of Na+ channels. In inside-out patches, with identical solutions at both membrane surfaces, there was a large
(40-50 mV) shift of channel opening and inactivation kinetics towards more negative potentials. However, for levels of comparable
opening probabilities, mean open times of Na+ channels were similar in cell-attached and inside-out patches. Tetrodotoxin
(10-20 microM) had no effect on Na+ channels when applied from the inside, but blocked them completely after application to
the outside membrane surface.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1983.sp014768</identifier><identifier>PMID: 6310098</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Action Potentials - drug effects ; Animals ; Cells, Cultured ; Ion Channels - drug effects ; Membrane Potentials - drug effects ; Myocardium - cytology ; Rats ; Sodium - physiology ; Tetrodotoxin - pharmacology</subject><ispartof>The Journal of physiology, 1983-07, Vol.340 (1), p.389-401</ispartof><rights>1983 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5669-3e74d7dfaa5c745dc474a219e46322029f4da02b6a39b3bab8db822fb870067a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1199215/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1199215/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1416,27923,27924,45573,45574,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6310098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cachelin, A B</creatorcontrib><creatorcontrib>De Peyer, J E</creatorcontrib><creatorcontrib>Kokubun, S</creatorcontrib><creatorcontrib>Reuter, H</creatorcontrib><title>Sodium channels in cultured cardiac cells</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>Primary cardiac cell cultures were prepared from the hearts of neonatal rats. The patch-clamp method (Hamill, Marty, Neher,
Sakmann & Sigworth, 1981) was applied for studying whole-cell Na+ currents and single-channel Na+ currents, respectively.
Whole-cell recordings yielded voltage- and time-dependent Na+ currents which could be blocked by tetrodotoxin. Single-channel
Na+ currents were directly compared in cell-attached patches and in inside-out patches. In cell-attached patches the elementary
current was about -1 pA at -10 mV and the slope conductance over a 50 mV voltage range was 15.1 +/- 1.6 pS (mean +/- S.D.).
Inactivation during depolarization and after conditioning clamp steps, in the steady state, resulted from a reduced opening
probability of Na+ channels. In inside-out patches, with identical solutions at both membrane surfaces, there was a large
(40-50 mV) shift of channel opening and inactivation kinetics towards more negative potentials. However, for levels of comparable
opening probabilities, mean open times of Na+ channels were similar in cell-attached and inside-out patches. Tetrodotoxin
(10-20 microM) had no effect on Na+ channels when applied from the inside, but blocked them completely after application to
the outside membrane surface.</description><subject>Action Potentials - drug effects</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Ion Channels - drug effects</subject><subject>Membrane Potentials - drug effects</subject><subject>Myocardium - cytology</subject><subject>Rats</subject><subject>Sodium - physiology</subject><subject>Tetrodotoxin - pharmacology</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1LwzAUhoMoc05_gtI78aIzX02aG0GHnwwUnNchTdI1o2tLszr6723pNvTOqwPnPe9z4AHgCsEpQojcrqqs9a7Mp0jEZOoriChn8REYI8pEyLkgx2AMIcYh4RE6BWferyBEBAoxAiNGEIQiHoObz9K4Zh3oTBWFzX3gikA3-aaprQm0qo1TOtA2z_05OElV7u3Fbk7A19PjYvYSzt-fX2f381BHrPtMLKeGm1SpSHMaGU05VRgJSxnBGGKRUqMgTpgiIiGJSmKTxBinScwhZFyRCbgbuFWTrK3RttjUKpdV7daqbmWpnPybFC6Ty_JbIiQERlEHYANA16X3tU0PXQRl707u3cnendy764qXvz8fajtZXf4w5FuX2_afVLl4--gXhHbyY9FBrgdI5pbZ1tVWDjVfamc3rezuJJL95Q_fW5Et</recordid><startdate>19830701</startdate><enddate>19830701</enddate><creator>Cachelin, A B</creator><creator>De Peyer, J E</creator><creator>Kokubun, S</creator><creator>Reuter, H</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19830701</creationdate><title>Sodium channels in cultured cardiac cells</title><author>Cachelin, A B ; De Peyer, J E ; Kokubun, S ; Reuter, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5669-3e74d7dfaa5c745dc474a219e46322029f4da02b6a39b3bab8db822fb870067a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Action Potentials - drug effects</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Ion Channels - drug effects</topic><topic>Membrane Potentials - drug effects</topic><topic>Myocardium - cytology</topic><topic>Rats</topic><topic>Sodium - physiology</topic><topic>Tetrodotoxin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cachelin, A B</creatorcontrib><creatorcontrib>De Peyer, J E</creatorcontrib><creatorcontrib>Kokubun, S</creatorcontrib><creatorcontrib>Reuter, H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cachelin, A B</au><au>De Peyer, J E</au><au>Kokubun, S</au><au>Reuter, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sodium channels in cultured cardiac cells</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1983-07-01</date><risdate>1983</risdate><volume>340</volume><issue>1</issue><spage>389</spage><epage>401</epage><pages>389-401</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>Primary cardiac cell cultures were prepared from the hearts of neonatal rats. The patch-clamp method (Hamill, Marty, Neher,
Sakmann & Sigworth, 1981) was applied for studying whole-cell Na+ currents and single-channel Na+ currents, respectively.
Whole-cell recordings yielded voltage- and time-dependent Na+ currents which could be blocked by tetrodotoxin. Single-channel
Na+ currents were directly compared in cell-attached patches and in inside-out patches. In cell-attached patches the elementary
current was about -1 pA at -10 mV and the slope conductance over a 50 mV voltage range was 15.1 +/- 1.6 pS (mean +/- S.D.).
Inactivation during depolarization and after conditioning clamp steps, in the steady state, resulted from a reduced opening
probability of Na+ channels. In inside-out patches, with identical solutions at both membrane surfaces, there was a large
(40-50 mV) shift of channel opening and inactivation kinetics towards more negative potentials. However, for levels of comparable
opening probabilities, mean open times of Na+ channels were similar in cell-attached and inside-out patches. Tetrodotoxin
(10-20 microM) had no effect on Na+ channels when applied from the inside, but blocked them completely after application to
the outside membrane surface.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>6310098</pmid><doi>10.1113/jphysiol.1983.sp014768</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3751 |
| ispartof | The Journal of physiology, 1983-07, Vol.340 (1), p.389-401 |
| issn | 0022-3751 1469-7793 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1199215 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Online Library All Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Action Potentials - drug effects Animals Cells, Cultured Ion Channels - drug effects Membrane Potentials - drug effects Myocardium - cytology Rats Sodium - physiology Tetrodotoxin - pharmacology |
| title | Sodium channels in cultured cardiac cells |
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