Differential susceptibility of fresh Trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus
The ability of complement in human menstrual blood and cervical mucus to kill Trichomonas vaginalis was compared with that of complement in serum, and 95 fresh trichomonal isolates obtained from vaginal wash material were evaluated for susceptibility to complement immediately after isolation. Only s...
Gespeichert in:
| Veröffentlicht in: | Genitourinary Medicine 1988-06, Vol.64 (3), p.176-179 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 179 |
|---|---|
| container_issue | 3 |
| container_start_page | 176 |
| container_title | Genitourinary Medicine |
| container_volume | 64 |
| creator | Demes, P Gombosová, A Valent, M Jánoska, A Fabusová, H Petrenko, M |
| description | The ability of complement in human menstrual blood and cervical mucus to kill Trichomonas vaginalis was compared with that of complement in serum, and 95 fresh trichomonal isolates obtained from vaginal wash material were evaluated for susceptibility to complement immediately after isolation. Only serum and menstrual blood with haemolytic activity produced total cytolysis of T vaginalis. The cytolytic abilities of these fluids were totally inactivated by treatment with heat or edetic acid (EDTA), which confirms the role of complement in cytolysis. Most cervical mucus samples had no detectable trichomonal cytotoxic properties. The cytotoxic activity in the remaining samples was not due to complement, as it was heat stable. Fresh isolates of T vaginalis and subpopulations of fresh isolates differed in their susceptibility or resistance to complement mediated lysis in serum. Resistance to complement did not remain stable after trichomonads were grown in vitro. |
| doi_str_mv | 10.1136/sti.64.3.176 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1194195</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>14966534</sourcerecordid><originalsourceid>FETCH-LOGICAL-b465t-ab7c847dfb203d5134d099ac202e4c7e5ec0155e995f1350db65ef3462b7c0c43</originalsourceid><addsrcrecordid>eNqFkc1vEzEQxS0EKmnhxhXJEhJcusFef60vSJACrShFQoEDF8vr9TYO3nWwvaH973GVKAIOcBrJ7zfPb2YAeILRHGPCX6bs5pzOyRwLfg_MMBV1RWpO7oMZqjmvKKHNQ3Cc0hohxAWTR-Co6FigZgZ-nrm-t9GO2WkP05SM3WTXOu_yLQw97KNNK7iMzqzCEEad4FZfu1F7l6BLwetsE8wBmjBsvB2KD3QjLDXlOBXH1ofQQT120Ni4daY8DZOZ0iPwoNc-2cf7egK-vHu7XJxXl5_eXyxeX1Yt5SxXuhWmoaLr2xqRjmFCOySlNjWqLTXCMmsQZsxKyXpMGOpazmxPKK9LIzKUnIBXO9_N1A62MyVg1F5toht0vFVBO_WnMrqVug5bhbGkWLJi8HxvEMOPyaasBleW5L0ebZiSEg1piBDyvyCmknNG7iI9-wtchymWlRZGCCQJJrgp1OmOMjGkFG1_yIyRuru7KndXnCpSunjBn_4-5wHeH7ro1U53Kdubg6zjd8UFEUxdfV2ozw3-9nH54Uq9KfyLHd8O63___AsQDMg4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770931318</pqid></control><display><type>article</type><title>Differential susceptibility of fresh Trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Demes, P ; Gombosová, A ; Valent, M ; Jánoska, A ; Fabusová, H ; Petrenko, M</creator><creatorcontrib>Demes, P ; Gombosová, A ; Valent, M ; Jánoska, A ; Fabusová, H ; Petrenko, M</creatorcontrib><description>The ability of complement in human menstrual blood and cervical mucus to kill Trichomonas vaginalis was compared with that of complement in serum, and 95 fresh trichomonal isolates obtained from vaginal wash material were evaluated for susceptibility to complement immediately after isolation. Only serum and menstrual blood with haemolytic activity produced total cytolysis of T vaginalis. The cytolytic abilities of these fluids were totally inactivated by treatment with heat or edetic acid (EDTA), which confirms the role of complement in cytolysis. Most cervical mucus samples had no detectable trichomonal cytotoxic properties. The cytotoxic activity in the remaining samples was not due to complement, as it was heat stable. Fresh isolates of T vaginalis and subpopulations of fresh isolates differed in their susceptibility or resistance to complement mediated lysis in serum. Resistance to complement did not remain stable after trichomonads were grown in vitro.</description><identifier>ISSN: 0266-4348</identifier><identifier>ISSN: 1368-4973</identifier><identifier>EISSN: 1472-3263</identifier><identifier>DOI: 10.1136/sti.64.3.176</identifier><identifier>PMID: 3261708</identifier><language>eng</language><publisher>England: BMJ Publishing Group Ltd</publisher><subject>Adolescent ; Adult ; Animals ; Cervix Mucus - immunology ; Complement System Proteins - physiology ; Cytotoxicity, Immunologic ; Female ; Humans ; Menstruation ; Middle Aged ; Trichomonas vaginalis</subject><ispartof>Genitourinary Medicine, 1988-06, Vol.64 (3), p.176-179</ispartof><rights>Copyright BMJ Publishing Group LTD Jun 1988</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-b465t-ab7c847dfb203d5134d099ac202e4c7e5ec0155e995f1350db65ef3462b7c0c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1194195/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1194195/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3261708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Demes, P</creatorcontrib><creatorcontrib>Gombosová, A</creatorcontrib><creatorcontrib>Valent, M</creatorcontrib><creatorcontrib>Jánoska, A</creatorcontrib><creatorcontrib>Fabusová, H</creatorcontrib><creatorcontrib>Petrenko, M</creatorcontrib><title>Differential susceptibility of fresh Trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus</title><title>Genitourinary Medicine</title><addtitle>Genitourin Med</addtitle><description>The ability of complement in human menstrual blood and cervical mucus to kill Trichomonas vaginalis was compared with that of complement in serum, and 95 fresh trichomonal isolates obtained from vaginal wash material were evaluated for susceptibility to complement immediately after isolation. Only serum and menstrual blood with haemolytic activity produced total cytolysis of T vaginalis. The cytolytic abilities of these fluids were totally inactivated by treatment with heat or edetic acid (EDTA), which confirms the role of complement in cytolysis. Most cervical mucus samples had no detectable trichomonal cytotoxic properties. The cytotoxic activity in the remaining samples was not due to complement, as it was heat stable. Fresh isolates of T vaginalis and subpopulations of fresh isolates differed in their susceptibility or resistance to complement mediated lysis in serum. Resistance to complement did not remain stable after trichomonads were grown in vitro.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Animals</subject><subject>Cervix Mucus - immunology</subject><subject>Complement System Proteins - physiology</subject><subject>Cytotoxicity, Immunologic</subject><subject>Female</subject><subject>Humans</subject><subject>Menstruation</subject><subject>Middle Aged</subject><subject>Trichomonas vaginalis</subject><issn>0266-4348</issn><issn>1368-4973</issn><issn>1472-3263</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1vEzEQxS0EKmnhxhXJEhJcusFef60vSJACrShFQoEDF8vr9TYO3nWwvaH973GVKAIOcBrJ7zfPb2YAeILRHGPCX6bs5pzOyRwLfg_MMBV1RWpO7oMZqjmvKKHNQ3Cc0hohxAWTR-Co6FigZgZ-nrm-t9GO2WkP05SM3WTXOu_yLQw97KNNK7iMzqzCEEad4FZfu1F7l6BLwetsE8wBmjBsvB2KD3QjLDXlOBXH1ofQQT120Ni4daY8DZOZ0iPwoNc-2cf7egK-vHu7XJxXl5_eXyxeX1Yt5SxXuhWmoaLr2xqRjmFCOySlNjWqLTXCMmsQZsxKyXpMGOpazmxPKK9LIzKUnIBXO9_N1A62MyVg1F5toht0vFVBO_WnMrqVug5bhbGkWLJi8HxvEMOPyaasBleW5L0ebZiSEg1piBDyvyCmknNG7iI9-wtchymWlRZGCCQJJrgp1OmOMjGkFG1_yIyRuru7KndXnCpSunjBn_4-5wHeH7ro1U53Kdubg6zjd8UFEUxdfV2ozw3-9nH54Uq9KfyLHd8O63___AsQDMg4</recordid><startdate>19880601</startdate><enddate>19880601</enddate><creator>Demes, P</creator><creator>Gombosová, A</creator><creator>Valent, M</creator><creator>Jánoska, A</creator><creator>Fabusová, H</creator><creator>Petrenko, M</creator><general>BMJ Publishing Group Ltd</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19880601</creationdate><title>Differential susceptibility of fresh Trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus</title><author>Demes, P ; Gombosová, A ; Valent, M ; Jánoska, A ; Fabusová, H ; Petrenko, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b465t-ab7c847dfb203d5134d099ac202e4c7e5ec0155e995f1350db65ef3462b7c0c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Animals</topic><topic>Cervix Mucus - immunology</topic><topic>Complement System Proteins - physiology</topic><topic>Cytotoxicity, Immunologic</topic><topic>Female</topic><topic>Humans</topic><topic>Menstruation</topic><topic>Middle Aged</topic><topic>Trichomonas vaginalis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demes, P</creatorcontrib><creatorcontrib>Gombosová, A</creatorcontrib><creatorcontrib>Valent, M</creatorcontrib><creatorcontrib>Jánoska, A</creatorcontrib><creatorcontrib>Fabusová, H</creatorcontrib><creatorcontrib>Petrenko, M</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genitourinary Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demes, P</au><au>Gombosová, A</au><au>Valent, M</au><au>Jánoska, A</au><au>Fabusová, H</au><au>Petrenko, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential susceptibility of fresh Trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus</atitle><jtitle>Genitourinary Medicine</jtitle><addtitle>Genitourin Med</addtitle><date>1988-06-01</date><risdate>1988</risdate><volume>64</volume><issue>3</issue><spage>176</spage><epage>179</epage><pages>176-179</pages><issn>0266-4348</issn><issn>1368-4973</issn><eissn>1472-3263</eissn><abstract>The ability of complement in human menstrual blood and cervical mucus to kill Trichomonas vaginalis was compared with that of complement in serum, and 95 fresh trichomonal isolates obtained from vaginal wash material were evaluated for susceptibility to complement immediately after isolation. Only serum and menstrual blood with haemolytic activity produced total cytolysis of T vaginalis. The cytolytic abilities of these fluids were totally inactivated by treatment with heat or edetic acid (EDTA), which confirms the role of complement in cytolysis. Most cervical mucus samples had no detectable trichomonal cytotoxic properties. The cytotoxic activity in the remaining samples was not due to complement, as it was heat stable. Fresh isolates of T vaginalis and subpopulations of fresh isolates differed in their susceptibility or resistance to complement mediated lysis in serum. Resistance to complement did not remain stable after trichomonads were grown in vitro.</abstract><cop>England</cop><pub>BMJ Publishing Group Ltd</pub><pmid>3261708</pmid><doi>10.1136/sti.64.3.176</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0266-4348 |
| ispartof | Genitourinary Medicine, 1988-06, Vol.64 (3), p.176-179 |
| issn | 0266-4348 1368-4973 1472-3263 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1194195 |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Adolescent Adult Animals Cervix Mucus - immunology Complement System Proteins - physiology Cytotoxicity, Immunologic Female Humans Menstruation Middle Aged Trichomonas vaginalis |
| title | Differential susceptibility of fresh Trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T00%3A39%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20susceptibility%20of%20fresh%20Trichomonas%20vaginalis%20isolates%20to%20complement%20in%20menstrual%20blood%20and%20cervical%20mucus&rft.jtitle=Genitourinary%20Medicine&rft.au=Demes,%20P&rft.date=1988-06-01&rft.volume=64&rft.issue=3&rft.spage=176&rft.epage=179&rft.pages=176-179&rft.issn=0266-4348&rft.eissn=1472-3263&rft_id=info:doi/10.1136/sti.64.3.176&rft_dat=%3Cproquest_pubme%3E14966534%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1770931318&rft_id=info:pmid/3261708&rfr_iscdi=true |