Acetylcholine regulation of nicotinic receptor channels through a putative G protein in chick myotubes
1. Single-channel currents induced by acetylcholine (ACh) were recorded from unstriated and non-innervated embryonic chick myotubes using the cell-attached patch-clamp technique. 2. ACh applied to the non-patched membrane decreased both channel opening probability and conductance. These ACh-induced...
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| Veröffentlicht in: | The Journal of physiology 1987-12, Vol.393 (1), p.635-645 |
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| creator | Eusebi, F Grassi, F Molinaro, M Zani, B M |
| description | 1. Single-channel currents induced by acetylcholine (ACh) were recorded from unstriated and non-innervated embryonic chick
myotubes using the cell-attached patch-clamp technique. 2. ACh applied to the non-patched membrane decreased both channel
opening probability and conductance. These ACh-induced effects occurred also when the non-patched membrane was exposed to
nominally Ca2+-free extracellular medium, but were absent when it was treated with curare. 3. ACh-induced membrane current
recorded under whole-cell patch-clamp conditions decreased in amplitude and time course when myotubes were intracellularly
loaded with guanosine-5'-O-(3-thiotriphosphate) GTP gamma S), but not with guanosine-5'-O-(2-thiodiphosphate) (GDP beta S)
or cyclic adenosine-5'-monophosphate (cyclic AMP). Internal perfusion of GTP gamma S affected the ACh-induced openings in
a similar manner to the non-patch ACh application. 4. These results suggest that ACh, in addition to its direct effect, acts
indirectly on the nicotinic receptor channels by delivering an intracellular messenger and through the activation of a putative
G protein. |
| doi_str_mv | 10.1113/jphysiol.1987.sp016845 |
| format | Article |
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myotubes using the cell-attached patch-clamp technique. 2. ACh applied to the non-patched membrane decreased both channel
opening probability and conductance. These ACh-induced effects occurred also when the non-patched membrane was exposed to
nominally Ca2+-free extracellular medium, but were absent when it was treated with curare. 3. ACh-induced membrane current
recorded under whole-cell patch-clamp conditions decreased in amplitude and time course when myotubes were intracellularly
loaded with guanosine-5'-O-(3-thiotriphosphate) GTP gamma S), but not with guanosine-5'-O-(2-thiodiphosphate) (GDP beta S)
or cyclic adenosine-5'-monophosphate (cyclic AMP). Internal perfusion of GTP gamma S affected the ACh-induced openings in
a similar manner to the non-patch ACh application. 4. These results suggest that ACh, in addition to its direct effect, acts
indirectly on the nicotinic receptor channels by delivering an intracellular messenger and through the activation of a putative
G protein.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1987.sp016845</identifier><identifier>PMID: 2451747</identifier><identifier>CODEN: JPHYA7</identifier><language>eng</language><publisher>Oxford: The Physiological Society</publisher><subject>acetylcholine ; Acetylcholine - pharmacology ; Action Potentials - drug effects ; Animals ; Biological and medical sciences ; Calcium - pharmacology ; Cells, Cultured ; Chick Embryo ; Cyclic AMP - pharmacology ; Embryology: invertebrates and vertebrates. Teratology ; Experimental organogenesis ; Fundamental and applied biological sciences. Psychology ; guanine nucleotide-binding proteins ; Guanosine 5'-O-(3-Thiotriphosphate) ; Guanosine Diphosphate - analogs & derivatives ; Guanosine Diphosphate - pharmacology ; Guanosine Triphosphate - analogs & derivatives ; Guanosine Triphosphate - pharmacology ; Ion Channels - drug effects ; membrane currents ; Muscles - embryology ; Muscles - physiology ; Nerve Tissue Proteins - physiology ; Organogenesis. Physiological fonctions ; Receptors, Nicotinic - physiology ; skeletal muscle ; Thionucleotides - pharmacology</subject><ispartof>The Journal of physiology, 1987-12, Vol.393 (1), p.635-645</ispartof><rights>1987 The Physiological Society</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5935-7bdda6c6daefb40bf260b65b624bc9ae3fd7b235db75d34c1af202e143c4f55b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1192415/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1192415/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1416,27923,27924,45573,45574,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7632094$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2451747$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eusebi, F</creatorcontrib><creatorcontrib>Grassi, F</creatorcontrib><creatorcontrib>Molinaro, M</creatorcontrib><creatorcontrib>Zani, B M</creatorcontrib><title>Acetylcholine regulation of nicotinic receptor channels through a putative G protein in chick myotubes</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. Single-channel currents induced by acetylcholine (ACh) were recorded from unstriated and non-innervated embryonic chick
myotubes using the cell-attached patch-clamp technique. 2. ACh applied to the non-patched membrane decreased both channel
opening probability and conductance. These ACh-induced effects occurred also when the non-patched membrane was exposed to
nominally Ca2+-free extracellular medium, but were absent when it was treated with curare. 3. ACh-induced membrane current
recorded under whole-cell patch-clamp conditions decreased in amplitude and time course when myotubes were intracellularly
loaded with guanosine-5'-O-(3-thiotriphosphate) GTP gamma S), but not with guanosine-5'-O-(2-thiodiphosphate) (GDP beta S)
or cyclic adenosine-5'-monophosphate (cyclic AMP). Internal perfusion of GTP gamma S affected the ACh-induced openings in
a similar manner to the non-patch ACh application. 4. These results suggest that ACh, in addition to its direct effect, acts
indirectly on the nicotinic receptor channels by delivering an intracellular messenger and through the activation of a putative
G protein.</description><subject>acetylcholine</subject><subject>Acetylcholine - pharmacology</subject><subject>Action Potentials - drug effects</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - pharmacology</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Cyclic AMP - pharmacology</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Experimental organogenesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>guanine nucleotide-binding proteins</subject><subject>Guanosine 5'-O-(3-Thiotriphosphate)</subject><subject>Guanosine Diphosphate - analogs & derivatives</subject><subject>Guanosine Diphosphate - pharmacology</subject><subject>Guanosine Triphosphate - analogs & derivatives</subject><subject>Guanosine Triphosphate - pharmacology</subject><subject>Ion Channels - drug effects</subject><subject>membrane currents</subject><subject>Muscles - embryology</subject><subject>Muscles - physiology</subject><subject>Nerve Tissue Proteins - physiology</subject><subject>Organogenesis. Physiological fonctions</subject><subject>Receptors, Nicotinic - physiology</subject><subject>skeletal muscle</subject><subject>Thionucleotides - pharmacology</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkl9vFCEUxSdGU2v1I2h4MOrLrlz-Li8mtdGqaaIP9ZkAw-xQ2WGEmTb77WWz242-qAmB5N7fOVxyaJoXgJcAQN_ejP22hBSXoFZyWUYMYsX4g-YUmFALKRV92JxiTMiCSg6Pmyel3GAMFCt10pwQxkEyedp0585P2-j6FMPgUfbrOZoppAGlDg3BpSnUvdadH6eUkevNMPhY0NTnNK97ZNA4T1Vx69ElGnOafBhQXa4P7gfabNM0W1-eNo86E4t_djjPmu8fP1xffFpcfb38fHF-tXBcUb6Qtm2NcKI1vrMM244IbAW3gjDrlPG0a6UllLdW8pYyB6YjmHhg1LGOc0vPmnd733G2G986P0zZRD3msDF5q5MJ-s_OEHq9TrcaQBEGvBq8Ohjk9HP2ZdKbUJyP0Qw-zUXLFcZ8RUQF3_wVBIkVSEWE_KcnsGpIJFRQ7EGXUynZd8fBAetd6vo-db1LXd-nXoXPf3_2UXaIufZfHvqmOBO7bAYXyhGTghKsWMXe77G7EP32Py_X11--7QpUURB0N8vrvUkf1v1dyF7vZSW5UL-arpwGvSN_ARR03sw</recordid><startdate>19871201</startdate><enddate>19871201</enddate><creator>Eusebi, F</creator><creator>Grassi, F</creator><creator>Molinaro, M</creator><creator>Zani, B M</creator><general>The Physiological Society</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19871201</creationdate><title>Acetylcholine regulation of nicotinic receptor channels through a putative G protein in chick myotubes</title><author>Eusebi, F ; Grassi, F ; Molinaro, M ; Zani, B M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5935-7bdda6c6daefb40bf260b65b624bc9ae3fd7b235db75d34c1af202e143c4f55b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>acetylcholine</topic><topic>Acetylcholine - pharmacology</topic><topic>Action Potentials - drug effects</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - pharmacology</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>Cyclic AMP - pharmacology</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Experimental organogenesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>guanine nucleotide-binding proteins</topic><topic>Guanosine 5'-O-(3-Thiotriphosphate)</topic><topic>Guanosine Diphosphate - analogs & derivatives</topic><topic>Guanosine Diphosphate - pharmacology</topic><topic>Guanosine Triphosphate - analogs & derivatives</topic><topic>Guanosine Triphosphate - pharmacology</topic><topic>Ion Channels - drug effects</topic><topic>membrane currents</topic><topic>Muscles - embryology</topic><topic>Muscles - physiology</topic><topic>Nerve Tissue Proteins - physiology</topic><topic>Organogenesis. Physiological fonctions</topic><topic>Receptors, Nicotinic - physiology</topic><topic>skeletal muscle</topic><topic>Thionucleotides - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eusebi, F</creatorcontrib><creatorcontrib>Grassi, F</creatorcontrib><creatorcontrib>Molinaro, M</creatorcontrib><creatorcontrib>Zani, B M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eusebi, F</au><au>Grassi, F</au><au>Molinaro, M</au><au>Zani, B M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acetylcholine regulation of nicotinic receptor channels through a putative G protein in chick myotubes</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1987-12-01</date><risdate>1987</risdate><volume>393</volume><issue>1</issue><spage>635</spage><epage>645</epage><pages>635-645</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. Single-channel currents induced by acetylcholine (ACh) were recorded from unstriated and non-innervated embryonic chick
myotubes using the cell-attached patch-clamp technique. 2. ACh applied to the non-patched membrane decreased both channel
opening probability and conductance. These ACh-induced effects occurred also when the non-patched membrane was exposed to
nominally Ca2+-free extracellular medium, but were absent when it was treated with curare. 3. ACh-induced membrane current
recorded under whole-cell patch-clamp conditions decreased in amplitude and time course when myotubes were intracellularly
loaded with guanosine-5'-O-(3-thiotriphosphate) GTP gamma S), but not with guanosine-5'-O-(2-thiodiphosphate) (GDP beta S)
or cyclic adenosine-5'-monophosphate (cyclic AMP). Internal perfusion of GTP gamma S affected the ACh-induced openings in
a similar manner to the non-patch ACh application. 4. These results suggest that ACh, in addition to its direct effect, acts
indirectly on the nicotinic receptor channels by delivering an intracellular messenger and through the activation of a putative
G protein.</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>2451747</pmid><doi>10.1113/jphysiol.1987.sp016845</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of physiology, 1987-12, Vol.393 (1), p.635-645 |
| issn | 0022-3751 1469-7793 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; IngentaConnect Free/Open Access Journals; Wiley Online Library All Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | acetylcholine Acetylcholine - pharmacology Action Potentials - drug effects Animals Biological and medical sciences Calcium - pharmacology Cells, Cultured Chick Embryo Cyclic AMP - pharmacology Embryology: invertebrates and vertebrates. Teratology Experimental organogenesis Fundamental and applied biological sciences. Psychology guanine nucleotide-binding proteins Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Diphosphate - analogs & derivatives Guanosine Diphosphate - pharmacology Guanosine Triphosphate - analogs & derivatives Guanosine Triphosphate - pharmacology Ion Channels - drug effects membrane currents Muscles - embryology Muscles - physiology Nerve Tissue Proteins - physiology Organogenesis. Physiological fonctions Receptors, Nicotinic - physiology skeletal muscle Thionucleotides - pharmacology |
| title | Acetylcholine regulation of nicotinic receptor channels through a putative G protein in chick myotubes |
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