Electrophysiological analysis of the inactivation of sympathetic transmitter in the guinea-pig vas deferens
1. The properties of junction potentials evoked by nerve stimulation and by local application of drugs, and currents evoked by nerve stimulation, in the smooth muscle cells of the guinea-pig vas deferens have been investigated. The effects of temperature on these responses have been studied using in...
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| Veröffentlicht in: | The Journal of physiology 1988-10, Vol.404 (1), p.349-364 |
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| description | 1. The properties of junction potentials evoked by nerve stimulation and by local application of drugs, and currents evoked
by nerve stimulation, in the smooth muscle cells of the guinea-pig vas deferens have been investigated. The effects of temperature
on these responses have been studied using intracellular and extracellular recording. 2. Local, brief (5-15 ms) application
of 10(-4) M-adenosine-5'-triphosphate (ATP) from glass micropipettes onto the surface of the vas deferens, using pressure
pulses (103-206 kPa), elicited a depolarization of the smooth muscle cell membranes which closely resembled the nerve stimulation-evoked
excitatory junction potential (EJP). 3. Local application of 10(-4) M-noradrenaline (NA) failed to produce any detectable
membrane potential response. Junction potentials elicited by a mixture of 10(-4) M-ATP and 10(-4) M-NA (ratio by volume 1:50)
in the drug ejection micropipette were similar in shape to those evoked by ATP alone. 4. Cooling the tissue from 35 to 25
degrees C did not significantly alter resting membrane potentials but resulted in a significant prolongation of the rising
and decaying phases of the EJPs. Fifty per cent decay times for EJPs at 35 and 25 degrees C were (mean +/- S.D.) 236 +/- 20
and 434 +/- 30 ms respectively (P less than 0.01). 5. Extracellularly recorded excitatory junction currents (EJCs) elicited
by nerve stimulation, believed to reflect the transmembrane current underlying the EJPs, were prolonged in parallel at low
temperatures (50% decay times of EJCs at 35 and 25 degrees C: 11.73 +/- 3.94 and 26.15 +/- 8.4 ms, respectively, P less than
0.01). 6. Junction potentials evoked by locally applied, exogenous ATP were also significantly prolonged by cooling (50% decay
times: 663 +/- 88 ms at 35 degrees C and 1955 +/- 79 ms at 25 degrees C, P less than 0.01). 7. Bath application of 10(-6)
M-alpha,beta-methylene ATP, the enzymatically stable, desensitizing analogue of ATP, reversibly abolished nerve-evoked EJPs.
Local application of 10(-6) M-alpha,beta-methylene ATP led to a prolonged depolarization of the smooth muscle cells lasting
between 20 and 60 s. 8. Junction potentials elicited by locally applied alpha,beta-methylene ATP were not prolonged or otherwise
significantly altered on cooling. The durations of the depolarizations were 46.0 +/- 12.1 s at 35 degrees C and 43.4 +/- 10.6
s at 25 degrees C (P greater than 0.1). |
| doi_str_mv | 10.1113/jphysiol.1988.sp017293 |
| format | Article |
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by nerve stimulation, in the smooth muscle cells of the guinea-pig vas deferens have been investigated. The effects of temperature
on these responses have been studied using intracellular and extracellular recording. 2. Local, brief (5-15 ms) application
of 10(-4) M-adenosine-5'-triphosphate (ATP) from glass micropipettes onto the surface of the vas deferens, using pressure
pulses (103-206 kPa), elicited a depolarization of the smooth muscle cell membranes which closely resembled the nerve stimulation-evoked
excitatory junction potential (EJP). 3. Local application of 10(-4) M-noradrenaline (NA) failed to produce any detectable
membrane potential response. Junction potentials elicited by a mixture of 10(-4) M-ATP and 10(-4) M-NA (ratio by volume 1:50)
in the drug ejection micropipette were similar in shape to those evoked by ATP alone. 4. Cooling the tissue from 35 to 25
degrees C did not significantly alter resting membrane potentials but resulted in a significant prolongation of the rising
and decaying phases of the EJPs. Fifty per cent decay times for EJPs at 35 and 25 degrees C were (mean +/- S.D.) 236 +/- 20
and 434 +/- 30 ms respectively (P less than 0.01). 5. Extracellularly recorded excitatory junction currents (EJCs) elicited
by nerve stimulation, believed to reflect the transmembrane current underlying the EJPs, were prolonged in parallel at low
temperatures (50% decay times of EJCs at 35 and 25 degrees C: 11.73 +/- 3.94 and 26.15 +/- 8.4 ms, respectively, P less than
0.01). 6. Junction potentials evoked by locally applied, exogenous ATP were also significantly prolonged by cooling (50% decay
times: 663 +/- 88 ms at 35 degrees C and 1955 +/- 79 ms at 25 degrees C, P less than 0.01). 7. Bath application of 10(-6)
M-alpha,beta-methylene ATP, the enzymatically stable, desensitizing analogue of ATP, reversibly abolished nerve-evoked EJPs.
Local application of 10(-6) M-alpha,beta-methylene ATP led to a prolonged depolarization of the smooth muscle cells lasting
between 20 and 60 s. 8. Junction potentials elicited by locally applied alpha,beta-methylene ATP were not prolonged or otherwise
significantly altered on cooling. The durations of the depolarizations were 46.0 +/- 12.1 s at 35 degrees C and 43.4 +/- 10.6
s at 25 degrees C (P greater than 0.1).</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1988.sp017293</identifier><identifier>PMID: 2908123</identifier><identifier>CODEN: JPHYA7</identifier><language>eng</language><publisher>Oxford: The Physiological Society</publisher><subject>Adenosine Triphosphate - analogs & derivatives ; Adenosine Triphosphate - pharmacology ; Animals ; ATP ; Biological and medical sciences ; Cold Temperature ; electrical stimulation ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Male ; membrane potential ; Membrane Potentials - drug effects ; Muscle, Smooth - physiology ; Neuroeffector Junction - physiology ; neuromuscular junctions ; Neurotransmitter Agents - physiology ; neurotransmitters ; Norepinephrine - pharmacology ; Sympathetic Nervous System - physiology ; Time Factors ; vas deferens ; Vas Deferens - innervation ; Vertebrates: urinary system</subject><ispartof>The Journal of physiology, 1988-10, Vol.404 (1), p.349-364</ispartof><rights>1988 The Physiological Society</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5609-f7ee7344fc63188e218cc81974bb2b220f47cc0af18ba0588f45a8b3281cffb13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1190829/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1190829/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,27901,27902,45550,45551,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6703316$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2908123$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cunnane, T C</creatorcontrib><creatorcontrib>Manchanda, R</creatorcontrib><title>Electrophysiological analysis of the inactivation of sympathetic transmitter in the guinea-pig vas deferens</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. The properties of junction potentials evoked by nerve stimulation and by local application of drugs, and currents evoked
by nerve stimulation, in the smooth muscle cells of the guinea-pig vas deferens have been investigated. The effects of temperature
on these responses have been studied using intracellular and extracellular recording. 2. Local, brief (5-15 ms) application
of 10(-4) M-adenosine-5'-triphosphate (ATP) from glass micropipettes onto the surface of the vas deferens, using pressure
pulses (103-206 kPa), elicited a depolarization of the smooth muscle cell membranes which closely resembled the nerve stimulation-evoked
excitatory junction potential (EJP). 3. Local application of 10(-4) M-noradrenaline (NA) failed to produce any detectable
membrane potential response. Junction potentials elicited by a mixture of 10(-4) M-ATP and 10(-4) M-NA (ratio by volume 1:50)
in the drug ejection micropipette were similar in shape to those evoked by ATP alone. 4. Cooling the tissue from 35 to 25
degrees C did not significantly alter resting membrane potentials but resulted in a significant prolongation of the rising
and decaying phases of the EJPs. Fifty per cent decay times for EJPs at 35 and 25 degrees C were (mean +/- S.D.) 236 +/- 20
and 434 +/- 30 ms respectively (P less than 0.01). 5. Extracellularly recorded excitatory junction currents (EJCs) elicited
by nerve stimulation, believed to reflect the transmembrane current underlying the EJPs, were prolonged in parallel at low
temperatures (50% decay times of EJCs at 35 and 25 degrees C: 11.73 +/- 3.94 and 26.15 +/- 8.4 ms, respectively, P less than
0.01). 6. Junction potentials evoked by locally applied, exogenous ATP were also significantly prolonged by cooling (50% decay
times: 663 +/- 88 ms at 35 degrees C and 1955 +/- 79 ms at 25 degrees C, P less than 0.01). 7. Bath application of 10(-6)
M-alpha,beta-methylene ATP, the enzymatically stable, desensitizing analogue of ATP, reversibly abolished nerve-evoked EJPs.
Local application of 10(-6) M-alpha,beta-methylene ATP led to a prolonged depolarization of the smooth muscle cells lasting
between 20 and 60 s. 8. Junction potentials elicited by locally applied alpha,beta-methylene ATP were not prolonged or otherwise
significantly altered on cooling. The durations of the depolarizations were 46.0 +/- 12.1 s at 35 degrees C and 43.4 +/- 10.6
s at 25 degrees C (P greater than 0.1).</description><subject>Adenosine Triphosphate - analogs & derivatives</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>ATP</subject><subject>Biological and medical sciences</subject><subject>Cold Temperature</subject><subject>electrical stimulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Male</subject><subject>membrane potential</subject><subject>Membrane Potentials - drug effects</subject><subject>Muscle, Smooth - physiology</subject><subject>Neuroeffector Junction - physiology</subject><subject>neuromuscular junctions</subject><subject>Neurotransmitter Agents - physiology</subject><subject>neurotransmitters</subject><subject>Norepinephrine - pharmacology</subject><subject>Sympathetic Nervous System - physiology</subject><subject>Time Factors</subject><subject>vas deferens</subject><subject>Vas Deferens - innervation</subject><subject>Vertebrates: urinary system</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxS0EKsvCRwDlgOCUxWMnsXNBgqrljyrBoZwtx7UTl2wcbO9W-fY4TbqCE5ws-_3mzXgeQq8A7wCAvrsduylY1--g5nwXRgyM1PQR2kBR1TljNX2MNhgTklNWwlP0LIRbjIHiuj5DZ6TGHAjdoJ8XvVbRu9XNtVbJPpOD7NM9ZM5ksdOZHaSK9iijdcP8Fqb9KJMQrcqil0PY2xi1T9w93h7soGU-2jY7ypDdaKO9HsJz9MTIPugX67lFPy4vrs8_51ffPn05_3CVq7LCdW6Y1owWhVEVBc41Aa4Uh5oVTUMaQrApmFJYGuCNxCXnpiglbyjhoIxpgG7R-8V3PDR7faP0kGbsxejtXvpJOGnF38pgO9G6owBIa0lb3KI3q4F3vw46RLG3Qem-l4N2hyAYZ6wsGf8nCCWmnNJ5pGoBlXcheG1O0wAWc57iIU8x5yke8kyFL__8y6lsDTDpr1ddhpScSWEoG05YxXBqXyXs44Ld2V5P_9lcXH_9Pj8UuABazGt5u5h0tu3urNdiKQtOWR0nkTgBYiZ_A2Vq0kg</recordid><startdate>19881001</startdate><enddate>19881001</enddate><creator>Cunnane, T C</creator><creator>Manchanda, R</creator><general>The Physiological Society</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19881001</creationdate><title>Electrophysiological analysis of the inactivation of sympathetic transmitter in the guinea-pig vas deferens</title><author>Cunnane, T C ; Manchanda, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5609-f7ee7344fc63188e218cc81974bb2b220f47cc0af18ba0588f45a8b3281cffb13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adenosine Triphosphate - analogs & derivatives</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>ATP</topic><topic>Biological and medical sciences</topic><topic>Cold Temperature</topic><topic>electrical stimulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Male</topic><topic>membrane potential</topic><topic>Membrane Potentials - drug effects</topic><topic>Muscle, Smooth - physiology</topic><topic>Neuroeffector Junction - physiology</topic><topic>neuromuscular junctions</topic><topic>Neurotransmitter Agents - physiology</topic><topic>neurotransmitters</topic><topic>Norepinephrine - pharmacology</topic><topic>Sympathetic Nervous System - physiology</topic><topic>Time Factors</topic><topic>vas deferens</topic><topic>Vas Deferens - innervation</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cunnane, T C</creatorcontrib><creatorcontrib>Manchanda, R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cunnane, T C</au><au>Manchanda, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrophysiological analysis of the inactivation of sympathetic transmitter in the guinea-pig vas deferens</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1988-10-01</date><risdate>1988</risdate><volume>404</volume><issue>1</issue><spage>349</spage><epage>364</epage><pages>349-364</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. The properties of junction potentials evoked by nerve stimulation and by local application of drugs, and currents evoked
by nerve stimulation, in the smooth muscle cells of the guinea-pig vas deferens have been investigated. The effects of temperature
on these responses have been studied using intracellular and extracellular recording. 2. Local, brief (5-15 ms) application
of 10(-4) M-adenosine-5'-triphosphate (ATP) from glass micropipettes onto the surface of the vas deferens, using pressure
pulses (103-206 kPa), elicited a depolarization of the smooth muscle cell membranes which closely resembled the nerve stimulation-evoked
excitatory junction potential (EJP). 3. Local application of 10(-4) M-noradrenaline (NA) failed to produce any detectable
membrane potential response. Junction potentials elicited by a mixture of 10(-4) M-ATP and 10(-4) M-NA (ratio by volume 1:50)
in the drug ejection micropipette were similar in shape to those evoked by ATP alone. 4. Cooling the tissue from 35 to 25
degrees C did not significantly alter resting membrane potentials but resulted in a significant prolongation of the rising
and decaying phases of the EJPs. Fifty per cent decay times for EJPs at 35 and 25 degrees C were (mean +/- S.D.) 236 +/- 20
and 434 +/- 30 ms respectively (P less than 0.01). 5. Extracellularly recorded excitatory junction currents (EJCs) elicited
by nerve stimulation, believed to reflect the transmembrane current underlying the EJPs, were prolonged in parallel at low
temperatures (50% decay times of EJCs at 35 and 25 degrees C: 11.73 +/- 3.94 and 26.15 +/- 8.4 ms, respectively, P less than
0.01). 6. Junction potentials evoked by locally applied, exogenous ATP were also significantly prolonged by cooling (50% decay
times: 663 +/- 88 ms at 35 degrees C and 1955 +/- 79 ms at 25 degrees C, P less than 0.01). 7. Bath application of 10(-6)
M-alpha,beta-methylene ATP, the enzymatically stable, desensitizing analogue of ATP, reversibly abolished nerve-evoked EJPs.
Local application of 10(-6) M-alpha,beta-methylene ATP led to a prolonged depolarization of the smooth muscle cells lasting
between 20 and 60 s. 8. Junction potentials elicited by locally applied alpha,beta-methylene ATP were not prolonged or otherwise
significantly altered on cooling. The durations of the depolarizations were 46.0 +/- 12.1 s at 35 degrees C and 43.4 +/- 10.6
s at 25 degrees C (P greater than 0.1).</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>2908123</pmid><doi>10.1113/jphysiol.1988.sp017293</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - pharmacology Animals ATP Biological and medical sciences Cold Temperature electrical stimulation Fundamental and applied biological sciences. Psychology Guinea Pigs Male membrane potential Membrane Potentials - drug effects Muscle, Smooth - physiology Neuroeffector Junction - physiology neuromuscular junctions Neurotransmitter Agents - physiology neurotransmitters Norepinephrine - pharmacology Sympathetic Nervous System - physiology Time Factors vas deferens Vas Deferens - innervation Vertebrates: urinary system |
| title | Electrophysiological analysis of the inactivation of sympathetic transmitter in the guinea-pig vas deferens |
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