Development of desensitization during repetitive end-plate activity and single end-plate currents in frog muscle
1. The amplitudes of end-plate currents (EPCs) in short trains of fifteen to seventeen EPCs at 10 Hz were depressed in the presence of 10 microM-proadifen when acetylcholinesterase (AChE) was inhibited. 2. The proadifen-induced EPC depression was voltage-dependent and the effect was more pronounced...
Gespeichert in:
| Veröffentlicht in: | The Journal of physiology 1989-05, Vol.412 (1), p.113-122 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 122 |
|---|---|
| container_issue | 1 |
| container_start_page | 113 |
| container_title | The Journal of physiology |
| container_volume | 412 |
| creator | Giniatullin, R A Khamitov, G Khazipov, R Magazanik, L G Nikolsky, E E Snetkov, V A Vyskocil, F |
| description | 1. The amplitudes of end-plate currents (EPCs) in short trains of fifteen to seventeen EPCs at 10 Hz were depressed in the
presence of 10 microM-proadifen when acetylcholinesterase (AChE) was inhibited. 2. The proadifen-induced EPC depression was
voltage-dependent and the effect was more pronounced at negative membrane potentials. 3. In the presence of proadifen, the
mean amplitude of miniature end-plate currents (MEPCs) was reduced by 36% 5 s after the EPC train as compared with MEPCs before
the train. 4. Without proadifen, but with inhibited AChE, an increase of temperature from 20 to 26 degrees C and elevation
of external Ca2+ from 1.8 to 2.5 mM led to EPC amplitude depression in the train, which was also potential-dependent. 5. After
AChE inhibition, proadifen (10 microM) progressively shortened MEPC decay without significant reduction of amplitude up to
40 min of exposition. MEPCs were not affected by proadifen when AChE was active. 6. It is concluded that these postsynaptic
effects of proadifen can be explained neither by its action on the resting acetylcholine receptors (AChR) nor on open ion
channels but are due to its desensitization-promoting action. |
| doi_str_mv | 10.1113/jphysiol.1989.sp017606 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1190566</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1732828533</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5093-8819227450faf363ddc09edb849d3226547af2508a5fbc0f12bd361e60c278463</originalsourceid><addsrcrecordid>eNqNkUtv1DAURiMEKkPhJ4C84bHJ4Efs2BskKG9VgkVZWx7nZsaVJ07tZKrw63GUaSkbYGXp3nOPr_0VxTOC14QQ9vqy303JBb8mSqp16jGpBRb3ihWphCrrWrH7xQpjSktWc_KweJTSJcaEYaVOihMqMJZUror-PRzAh34P3YBCixpI0CU3uJ9mcKFDzRhdt0URehhy9QAIuqbsvRkAGZsLbpiQ6RqUMubvdu0YY5Ym5DrUxrBF-zFZD4-LB63xCZ4cz9Pix8cPF2efy_Nvn76cvT0vLceKlVISRWldcdyalgnWNBYraDayUg2jVPCqNi3lWBrebixuCd00TBAQ2NJaVoKdFm8Wbz9u9tDYvEo0XvfR7U2cdDBO_9np3E5vw0ETojAXs-DFURDD1Qhp0HuXLHhvOghj0vmHlVIVz-Crv4KkZjT_NWfsn07CmZJc0gyKBbQxpBShvV2cYD3nr2_y13P--ib_PPj07rNvx46B5_7zY98ka3wbTWdd-m1XTJJaqsy9W7hr52H6z9v1xdfvc6EidKay5OUi2bnt7tpF0MtYCtbBMOnMaaJn8hc1ouEk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15398582</pqid></control><display><type>article</type><title>Development of desensitization during repetitive end-plate activity and single end-plate currents in frog muscle</title><source>MEDLINE</source><source>IngentaConnect Free/Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Giniatullin, R A ; Khamitov, G ; Khazipov, R ; Magazanik, L G ; Nikolsky, E E ; Snetkov, V A ; Vyskocil, F</creator><creatorcontrib>Giniatullin, R A ; Khamitov, G ; Khazipov, R ; Magazanik, L G ; Nikolsky, E E ; Snetkov, V A ; Vyskocil, F</creatorcontrib><description>1. The amplitudes of end-plate currents (EPCs) in short trains of fifteen to seventeen EPCs at 10 Hz were depressed in the
presence of 10 microM-proadifen when acetylcholinesterase (AChE) was inhibited. 2. The proadifen-induced EPC depression was
voltage-dependent and the effect was more pronounced at negative membrane potentials. 3. In the presence of proadifen, the
mean amplitude of miniature end-plate currents (MEPCs) was reduced by 36% 5 s after the EPC train as compared with MEPCs before
the train. 4. Without proadifen, but with inhibited AChE, an increase of temperature from 20 to 26 degrees C and elevation
of external Ca2+ from 1.8 to 2.5 mM led to EPC amplitude depression in the train, which was also potential-dependent. 5. After
AChE inhibition, proadifen (10 microM) progressively shortened MEPC decay without significant reduction of amplitude up to
40 min of exposition. MEPCs were not affected by proadifen when AChE was active. 6. It is concluded that these postsynaptic
effects of proadifen can be explained neither by its action on the resting acetylcholine receptors (AChR) nor on open ion
channels but are due to its desensitization-promoting action.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1989.sp017606</identifier><identifier>PMID: 2600828</identifier><identifier>CODEN: JPHYA7</identifier><language>eng</language><publisher>Oxford: The Physiological Society</publisher><subject>Animals ; Anura ; Biological and medical sciences ; Cholinesterase Inhibitors - pharmacology ; Electrophysiology ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Motor Endplate - drug effects ; Motor Endplate - physiology ; Neuromuscular Junction - physiology ; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ ; proadifen ; Proadifen - pharmacology ; Rana ; Rana ridibunda ; Rana temporaria ; Receptors, Cholinergic - drug effects ; Receptors, Cholinergic - physiology ; skeletal muscle ; Vertebrates: nervous system and sense organs</subject><ispartof>The Journal of physiology, 1989-05, Vol.412 (1), p.113-122</ispartof><rights>1989 The Physiological Society</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5093-8819227450faf363ddc09edb849d3226547af2508a5fbc0f12bd361e60c278463</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1190566/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1190566/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,27924,27925,45574,45575,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19381789$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2600828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Giniatullin, R A</creatorcontrib><creatorcontrib>Khamitov, G</creatorcontrib><creatorcontrib>Khazipov, R</creatorcontrib><creatorcontrib>Magazanik, L G</creatorcontrib><creatorcontrib>Nikolsky, E E</creatorcontrib><creatorcontrib>Snetkov, V A</creatorcontrib><creatorcontrib>Vyskocil, F</creatorcontrib><title>Development of desensitization during repetitive end-plate activity and single end-plate currents in frog muscle</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. The amplitudes of end-plate currents (EPCs) in short trains of fifteen to seventeen EPCs at 10 Hz were depressed in the
presence of 10 microM-proadifen when acetylcholinesterase (AChE) was inhibited. 2. The proadifen-induced EPC depression was
voltage-dependent and the effect was more pronounced at negative membrane potentials. 3. In the presence of proadifen, the
mean amplitude of miniature end-plate currents (MEPCs) was reduced by 36% 5 s after the EPC train as compared with MEPCs before
the train. 4. Without proadifen, but with inhibited AChE, an increase of temperature from 20 to 26 degrees C and elevation
of external Ca2+ from 1.8 to 2.5 mM led to EPC amplitude depression in the train, which was also potential-dependent. 5. After
AChE inhibition, proadifen (10 microM) progressively shortened MEPC decay without significant reduction of amplitude up to
40 min of exposition. MEPCs were not affected by proadifen when AChE was active. 6. It is concluded that these postsynaptic
effects of proadifen can be explained neither by its action on the resting acetylcholine receptors (AChR) nor on open ion
channels but are due to its desensitization-promoting action.</description><subject>Animals</subject><subject>Anura</subject><subject>Biological and medical sciences</subject><subject>Cholinesterase Inhibitors - pharmacology</subject><subject>Electrophysiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Motor Endplate - drug effects</subject><subject>Motor Endplate - physiology</subject><subject>Neuromuscular Junction - physiology</subject><subject>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</subject><subject>proadifen</subject><subject>Proadifen - pharmacology</subject><subject>Rana</subject><subject>Rana ridibunda</subject><subject>Rana temporaria</subject><subject>Receptors, Cholinergic - drug effects</subject><subject>Receptors, Cholinergic - physiology</subject><subject>skeletal muscle</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAURiMEKkPhJ4C84bHJ4Efs2BskKG9VgkVZWx7nZsaVJ07tZKrw63GUaSkbYGXp3nOPr_0VxTOC14QQ9vqy303JBb8mSqp16jGpBRb3ihWphCrrWrH7xQpjSktWc_KweJTSJcaEYaVOihMqMJZUror-PRzAh34P3YBCixpI0CU3uJ9mcKFDzRhdt0URehhy9QAIuqbsvRkAGZsLbpiQ6RqUMubvdu0YY5Ym5DrUxrBF-zFZD4-LB63xCZ4cz9Pix8cPF2efy_Nvn76cvT0vLceKlVISRWldcdyalgnWNBYraDayUg2jVPCqNi3lWBrebixuCd00TBAQ2NJaVoKdFm8Wbz9u9tDYvEo0XvfR7U2cdDBO_9np3E5vw0ETojAXs-DFURDD1Qhp0HuXLHhvOghj0vmHlVIVz-Crv4KkZjT_NWfsn07CmZJc0gyKBbQxpBShvV2cYD3nr2_y13P--ib_PPj07rNvx46B5_7zY98ka3wbTWdd-m1XTJJaqsy9W7hr52H6z9v1xdfvc6EidKay5OUi2bnt7tpF0MtYCtbBMOnMaaJn8hc1ouEk</recordid><startdate>19890501</startdate><enddate>19890501</enddate><creator>Giniatullin, R A</creator><creator>Khamitov, G</creator><creator>Khazipov, R</creator><creator>Magazanik, L G</creator><creator>Nikolsky, E E</creator><creator>Snetkov, V A</creator><creator>Vyskocil, F</creator><general>The Physiological Society</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19890501</creationdate><title>Development of desensitization during repetitive end-plate activity and single end-plate currents in frog muscle</title><author>Giniatullin, R A ; Khamitov, G ; Khazipov, R ; Magazanik, L G ; Nikolsky, E E ; Snetkov, V A ; Vyskocil, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5093-8819227450faf363ddc09edb849d3226547af2508a5fbc0f12bd361e60c278463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Anura</topic><topic>Biological and medical sciences</topic><topic>Cholinesterase Inhibitors - pharmacology</topic><topic>Electrophysiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Motor Endplate - drug effects</topic><topic>Motor Endplate - physiology</topic><topic>Neuromuscular Junction - physiology</topic><topic>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</topic><topic>proadifen</topic><topic>Proadifen - pharmacology</topic><topic>Rana</topic><topic>Rana ridibunda</topic><topic>Rana temporaria</topic><topic>Receptors, Cholinergic - drug effects</topic><topic>Receptors, Cholinergic - physiology</topic><topic>skeletal muscle</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giniatullin, R A</creatorcontrib><creatorcontrib>Khamitov, G</creatorcontrib><creatorcontrib>Khazipov, R</creatorcontrib><creatorcontrib>Magazanik, L G</creatorcontrib><creatorcontrib>Nikolsky, E E</creatorcontrib><creatorcontrib>Snetkov, V A</creatorcontrib><creatorcontrib>Vyskocil, F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giniatullin, R A</au><au>Khamitov, G</au><au>Khazipov, R</au><au>Magazanik, L G</au><au>Nikolsky, E E</au><au>Snetkov, V A</au><au>Vyskocil, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of desensitization during repetitive end-plate activity and single end-plate currents in frog muscle</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1989-05-01</date><risdate>1989</risdate><volume>412</volume><issue>1</issue><spage>113</spage><epage>122</epage><pages>113-122</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. The amplitudes of end-plate currents (EPCs) in short trains of fifteen to seventeen EPCs at 10 Hz were depressed in the
presence of 10 microM-proadifen when acetylcholinesterase (AChE) was inhibited. 2. The proadifen-induced EPC depression was
voltage-dependent and the effect was more pronounced at negative membrane potentials. 3. In the presence of proadifen, the
mean amplitude of miniature end-plate currents (MEPCs) was reduced by 36% 5 s after the EPC train as compared with MEPCs before
the train. 4. Without proadifen, but with inhibited AChE, an increase of temperature from 20 to 26 degrees C and elevation
of external Ca2+ from 1.8 to 2.5 mM led to EPC amplitude depression in the train, which was also potential-dependent. 5. After
AChE inhibition, proadifen (10 microM) progressively shortened MEPC decay without significant reduction of amplitude up to
40 min of exposition. MEPCs were not affected by proadifen when AChE was active. 6. It is concluded that these postsynaptic
effects of proadifen can be explained neither by its action on the resting acetylcholine receptors (AChR) nor on open ion
channels but are due to its desensitization-promoting action.</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>2600828</pmid><doi>10.1113/jphysiol.1989.sp017606</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3751 |
| ispartof | The Journal of physiology, 1989-05, Vol.412 (1), p.113-122 |
| issn | 0022-3751 1469-7793 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1190566 |
| source | MEDLINE; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Anura Biological and medical sciences Cholinesterase Inhibitors - pharmacology Electrophysiology Fundamental and applied biological sciences. Psychology In Vitro Techniques Motor Endplate - drug effects Motor Endplate - physiology Neuromuscular Junction - physiology Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ proadifen Proadifen - pharmacology Rana Rana ridibunda Rana temporaria Receptors, Cholinergic - drug effects Receptors, Cholinergic - physiology skeletal muscle Vertebrates: nervous system and sense organs |
| title | Development of desensitization during repetitive end-plate activity and single end-plate currents in frog muscle |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T14%3A49%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20desensitization%20during%20repetitive%20end-plate%20activity%20and%20single%20end-plate%20currents%20in%20frog%20muscle&rft.jtitle=The%20Journal%20of%20physiology&rft.au=Giniatullin,%20R%20A&rft.date=1989-05-01&rft.volume=412&rft.issue=1&rft.spage=113&rft.epage=122&rft.pages=113-122&rft.issn=0022-3751&rft.eissn=1469-7793&rft.coden=JPHYA7&rft_id=info:doi/10.1113/jphysiol.1989.sp017606&rft_dat=%3Cproquest_pubme%3E1732828533%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15398582&rft_id=info:pmid/2600828&rfr_iscdi=true |