Efficacy of a transmissible gastroenteritis coronavirus with an altered ORF-3 gene
Serial passage of virulent transmissible gastroenteritis virus through cell culture reduced its virulence in 3-day-old piglets. Intramuscular inoculation of pregnant gilts with 2 doses of this modified-live virus elicited a level of lactogenic immunity that protected their nursing piglets against a...
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| Veröffentlicht in: | Canadian journal of veterinary research 2001, Vol.65 (1), p.28-32 |
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| creator | Woods, R.D |
| description | Serial passage of virulent transmissible gastroenteritis virus through cell culture reduced its virulence in 3-day-old piglets. Intramuscular inoculation of pregnant gilts with 2 doses of this modified-live virus elicited a level of lactogenic immunity that protected their nursing piglets against a lethal dose of challenge virus. Sequence analysis of a 637-bp fragment of the spike gene containing most of the aminopeptidase receptor and the 4 major antigenic sites from the original and the serially passed viruses were nearly identical. Gel analysis revealed that the fragment from the ORF-3 gene of virulent virus was smaller than the corresponding fragment from the serially passed virus. Sequence analysis of the fragment from the passed virus revealed that the sequence between nt 5310 and nt 5434 was replaced by a 636-bp fragment from the polymerase 1A gene. This replacement resulted in the loss of the CTAAACTT leader RNA-binding site and ATG start codon for the ORF-3A gene but it did not affect the ORF-3B gene. |
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Intramuscular inoculation of pregnant gilts with 2 doses of this modified-live virus elicited a level of lactogenic immunity that protected their nursing piglets against a lethal dose of challenge virus. Sequence analysis of a 637-bp fragment of the spike gene containing most of the aminopeptidase receptor and the 4 major antigenic sites from the original and the serially passed viruses were nearly identical. Gel analysis revealed that the fragment from the ORF-3 gene of virulent virus was smaller than the corresponding fragment from the serially passed virus. Sequence analysis of the fragment from the passed virus revealed that the sequence between nt 5310 and nt 5434 was replaced by a 636-bp fragment from the polymerase 1A gene. This replacement resulted in the loss of the CTAAACTT leader RNA-binding site and ATG start codon for the ORF-3A gene but it did not affect the ORF-3B gene.</description><identifier>ISSN: 0830-9000</identifier><identifier>PMID: 11227191</identifier><language>eng</language><publisher>Canada</publisher><subject>Animals ; Animals, Newborn ; Animals, Suckling ; Antibodies, Viral - biosynthesis ; Antibodies, Viral - blood ; antibody formation ; Base Sequence ; DNA Primers ; Female ; Gastroenteritis, Transmissible, of Swine - immunology ; Gastroenteritis, Transmissible, of Swine - prevention & control ; Immunity, Maternally-Acquired - immunology ; live vaccines ; maternal immunity ; Milk - immunology ; Molecular Sequence Data ; Molecular Weight ; morbidity ; mortality ; nucleotide sequences ; piglets ; polymerase chain reaction ; Pregnancy ; Reverse Transcriptase Polymerase Chain Reaction ; Serial Passage - veterinary ; sows ; Swine ; Transmissible gastroenteritis virus ; Transmissible gastroenteritis virus - genetics ; Transmissible gastroenteritis virus - immunology ; Transmissible gastroenteritis virus - pathogenicity ; vaccination ; vaccine development ; Viral Vaccines - genetics ; Viral Vaccines - immunology ; Viral Vaccines - standards ; virulence ; Virulence - genetics ; Virulence - immunology</subject><ispartof>Canadian journal of veterinary research, 2001, Vol.65 (1), p.28-32</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1189638/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1189638/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,4025,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11227191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woods, R.D</creatorcontrib><title>Efficacy of a transmissible gastroenteritis coronavirus with an altered ORF-3 gene</title><title>Canadian journal of veterinary research</title><addtitle>Can J Vet Res</addtitle><description>Serial passage of virulent transmissible gastroenteritis virus through cell culture reduced its virulence in 3-day-old piglets. Intramuscular inoculation of pregnant gilts with 2 doses of this modified-live virus elicited a level of lactogenic immunity that protected their nursing piglets against a lethal dose of challenge virus. Sequence analysis of a 637-bp fragment of the spike gene containing most of the aminopeptidase receptor and the 4 major antigenic sites from the original and the serially passed viruses were nearly identical. Gel analysis revealed that the fragment from the ORF-3 gene of virulent virus was smaller than the corresponding fragment from the serially passed virus. Sequence analysis of the fragment from the passed virus revealed that the sequence between nt 5310 and nt 5434 was replaced by a 636-bp fragment from the polymerase 1A gene. This replacement resulted in the loss of the CTAAACTT leader RNA-binding site and ATG start codon for the ORF-3A gene but it did not affect the ORF-3B gene.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Animals, Suckling</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antibodies, Viral - blood</subject><subject>antibody formation</subject><subject>Base Sequence</subject><subject>DNA Primers</subject><subject>Female</subject><subject>Gastroenteritis, Transmissible, of Swine - immunology</subject><subject>Gastroenteritis, Transmissible, of Swine - prevention & control</subject><subject>Immunity, Maternally-Acquired - immunology</subject><subject>live vaccines</subject><subject>maternal immunity</subject><subject>Milk - immunology</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>morbidity</subject><subject>mortality</subject><subject>nucleotide sequences</subject><subject>piglets</subject><subject>polymerase chain reaction</subject><subject>Pregnancy</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Serial Passage - veterinary</subject><subject>sows</subject><subject>Swine</subject><subject>Transmissible gastroenteritis virus</subject><subject>Transmissible gastroenteritis virus - genetics</subject><subject>Transmissible gastroenteritis virus - immunology</subject><subject>Transmissible gastroenteritis virus - pathogenicity</subject><subject>vaccination</subject><subject>vaccine development</subject><subject>Viral Vaccines - genetics</subject><subject>Viral Vaccines - immunology</subject><subject>Viral Vaccines - standards</subject><subject>virulence</subject><subject>Virulence - genetics</subject><subject>Virulence - immunology</subject><issn>0830-9000</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1LAzEQhveg2Fr9C5qTt4V8dJPNRZDSqlAoVHsOk2yyjWw3Ndmt9N-70Cp6msP78Lwzc5GNcclwLjHGo-w6pQ-MqeCUXWUjQigVRJJxtp475w2YIwoOAeoitGnnU_K6saiG1MVg285G3_mETIihhYOPfUJfvtsiaBE0Q2ortFovcoZq29qb7NJBk-zteU6yzWL-PnvJl6vn19nTMne05F0OzjhqiikYpoXDWuiysNYUIKtpgTmXWuIpk06XQJiQEkprRKU5LjkV2hI2yR5P3n2vd7Yyw54RGrWPfgfxqAJ49T9p_VbV4aAIKSVn5SB4OAti-Oxt6tRwubFNA60NfVICc8pxUQzg3d-m34qfNw7A_QlwEBTU0Se1eaOYMEwl5WIgvgG2U3jx</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Woods, R.D</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2001</creationdate><title>Efficacy of a transmissible gastroenteritis coronavirus with an altered ORF-3 gene</title><author>Woods, R.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f286t-afcf2c54ac3b7f0b7b85eec5a9d450669b90439fb8a13799a8ec7db608627be13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Animals, Suckling</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antibodies, Viral - blood</topic><topic>antibody formation</topic><topic>Base Sequence</topic><topic>DNA Primers</topic><topic>Female</topic><topic>Gastroenteritis, Transmissible, of Swine - immunology</topic><topic>Gastroenteritis, Transmissible, of Swine - prevention & control</topic><topic>Immunity, Maternally-Acquired - immunology</topic><topic>live vaccines</topic><topic>maternal immunity</topic><topic>Milk - immunology</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>morbidity</topic><topic>mortality</topic><topic>nucleotide sequences</topic><topic>piglets</topic><topic>polymerase chain reaction</topic><topic>Pregnancy</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Serial Passage - veterinary</topic><topic>sows</topic><topic>Swine</topic><topic>Transmissible gastroenteritis virus</topic><topic>Transmissible gastroenteritis virus - genetics</topic><topic>Transmissible gastroenteritis virus - immunology</topic><topic>Transmissible gastroenteritis virus - pathogenicity</topic><topic>vaccination</topic><topic>vaccine development</topic><topic>Viral Vaccines - genetics</topic><topic>Viral Vaccines - immunology</topic><topic>Viral Vaccines - standards</topic><topic>virulence</topic><topic>Virulence - genetics</topic><topic>Virulence - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woods, R.D</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Canadian journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woods, R.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficacy of a transmissible gastroenteritis coronavirus with an altered ORF-3 gene</atitle><jtitle>Canadian journal of veterinary research</jtitle><addtitle>Can J Vet Res</addtitle><date>2001</date><risdate>2001</risdate><volume>65</volume><issue>1</issue><spage>28</spage><epage>32</epage><pages>28-32</pages><issn>0830-9000</issn><abstract>Serial passage of virulent transmissible gastroenteritis virus through cell culture reduced its virulence in 3-day-old piglets. Intramuscular inoculation of pregnant gilts with 2 doses of this modified-live virus elicited a level of lactogenic immunity that protected their nursing piglets against a lethal dose of challenge virus. Sequence analysis of a 637-bp fragment of the spike gene containing most of the aminopeptidase receptor and the 4 major antigenic sites from the original and the serially passed viruses were nearly identical. Gel analysis revealed that the fragment from the ORF-3 gene of virulent virus was smaller than the corresponding fragment from the serially passed virus. Sequence analysis of the fragment from the passed virus revealed that the sequence between nt 5310 and nt 5434 was replaced by a 636-bp fragment from the polymerase 1A gene. This replacement resulted in the loss of the CTAAACTT leader RNA-binding site and ATG start codon for the ORF-3A gene but it did not affect the ORF-3B gene.</abstract><cop>Canada</cop><pmid>11227191</pmid><tpages>5</tpages></addata></record> |
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| ispartof | Canadian journal of veterinary research, 2001, Vol.65 (1), p.28-32 |
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| subjects | Animals Animals, Newborn Animals, Suckling Antibodies, Viral - biosynthesis Antibodies, Viral - blood antibody formation Base Sequence DNA Primers Female Gastroenteritis, Transmissible, of Swine - immunology Gastroenteritis, Transmissible, of Swine - prevention & control Immunity, Maternally-Acquired - immunology live vaccines maternal immunity Milk - immunology Molecular Sequence Data Molecular Weight morbidity mortality nucleotide sequences piglets polymerase chain reaction Pregnancy Reverse Transcriptase Polymerase Chain Reaction Serial Passage - veterinary sows Swine Transmissible gastroenteritis virus Transmissible gastroenteritis virus - genetics Transmissible gastroenteritis virus - immunology Transmissible gastroenteritis virus - pathogenicity vaccination vaccine development Viral Vaccines - genetics Viral Vaccines - immunology Viral Vaccines - standards virulence Virulence - genetics Virulence - immunology |
| title | Efficacy of a transmissible gastroenteritis coronavirus with an altered ORF-3 gene |
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