Production of a baculovirus-derived gp50 protein and utilization in a competitive enzyme-linked immunosorbent assay for the serodiagnosis of pseudorabies virus

The pseudorabies virus (PRV) gp50 envelope glycoprotein gene was cloned and expressed in a recombinant baculovirus. An anti-gp50 Mab (1842) recognized a protein of approximately 40 kDa in immunoblotting assays from infected insect cell lysates, while this product was not present in cells infected wi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Canadian journal of veterinary research 1997-10, Vol.61 (4), p.286-291
Hauptverfasser:	Prud'Homme, I, Zhou, E.M, Traykova, M, Trotter, H, Chan, M, Afshar, A, Harding, M.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - blood Antibodies, Viral - immunology Antibody Specificity Antigens, Viral - immunology Baculoviridae - metabolism Cell Line Cercopithecus aethiops Chromatography, Affinity - methods Chromatography, Affinity - veterinary Electrophoresis, Polyacrylamide Gel - methods Electrophoresis, Polyacrylamide Gel - veterinary enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Genetic Vectors Herpesvirus 1, Suid - immunology immunoblotting Immunoblotting - methods Immunoblotting - veterinary Kidney - cytology Kidney - virology Moths - cytology Moths - virology neutralization tests Neutralization Tests - methods Neutralization Tests - veterinary plasmid vectors polyacrylamide gel electrophoresis Pseudorabies - blood Pseudorabies - diagnosis Pseudorabies - immunology purification recombinant antigens Recombinant Proteins - metabolism Sensitivity and Specificity Suid herpesvirus 1 Swine Swine Diseases - blood Swine Diseases - diagnosis Swine Diseases - immunology Vero Cells Viral Envelope Proteins - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!