A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner
An insecticidal exotoxin from Bacillus thuringiensis var. thuringiensis (Berliner) has been purified. The efficiency of each stage of the purification has been ascertained and the yield of toxic material estimated by means of a quantitative bioassay. It is shown that the exotoxin is an adenine deriv...
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| Veröffentlicht in: | Biochemical journal 1969-09, Vol.114 (3), p.477-488 |
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| container_title | Biochemical journal |
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| creator | Bond, R P Boyce, C B French, S J |
| description | An insecticidal exotoxin from Bacillus thuringiensis var. thuringiensis (Berliner) has been purified. The efficiency of each stage of the purification has been ascertained and the yield of toxic material estimated by means of a quantitative bioassay. It is shown that the exotoxin is an adenine derivative substituted at position 9 and having a molecular weight of approximately 825. It can be dephosphorylated enzymically or chemically under conditions that define the exotoxin as a phosphomonoester. This results in loss of toxicity, both to insects and to mice. Spectroscopic and kinetic data are presented which suggest that a beta-ribofuranosyl moiety may be attached to the adenine. Glucose and allomucic acid have been positively identified as hydrolysis fragments from the exotoxin. These results are discussed and compared with the results of others on similar (or possibly identical) compounds. |
| doi_str_mv | 10.1042/bj1140477 |
| format | Article |
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The efficiency of each stage of the purification has been ascertained and the yield of toxic material estimated by means of a quantitative bioassay. It is shown that the exotoxin is an adenine derivative substituted at position 9 and having a molecular weight of approximately 825. It can be dephosphorylated enzymically or chemically under conditions that define the exotoxin as a phosphomonoester. This results in loss of toxicity, both to insects and to mice. Spectroscopic and kinetic data are presented which suggest that a beta-ribofuranosyl moiety may be attached to the adenine. Glucose and allomucic acid have been positively identified as hydrolysis fragments from the exotoxin. These results are discussed and compared with the results of others on similar (or possibly identical) compounds.</description><identifier>ISSN: 0264-6021</identifier><identifier>ISSN: 0306-3283</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj1140477</identifier><identifier>PMID: 5820635</identifier><language>eng</language><publisher>England</publisher><subject>Adenine - analysis ; Bacillus - analysis ; Biological Assay ; Insecta ; Kinetics ; Molecular Weight ; Phosphates - analysis ; Ribose - analysis ; Spectrum Analysis ; Toxins, Biological - analysis ; Toxins, Biological - isolation & purification</subject><ispartof>Biochemical journal, 1969-09, Vol.114 (3), p.477-488</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-3411aeaaa533159510adba59c68724ba179a9349c65fdb042a97757b44f111e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1184919/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1184919/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5820635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bond, R P</creatorcontrib><creatorcontrib>Boyce, C B</creatorcontrib><creatorcontrib>French, S J</creatorcontrib><title>A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>An insecticidal exotoxin from Bacillus thuringiensis var. thuringiensis (Berliner) has been purified. The efficiency of each stage of the purification has been ascertained and the yield of toxic material estimated by means of a quantitative bioassay. It is shown that the exotoxin is an adenine derivative substituted at position 9 and having a molecular weight of approximately 825. It can be dephosphorylated enzymically or chemically under conditions that define the exotoxin as a phosphomonoester. This results in loss of toxicity, both to insects and to mice. Spectroscopic and kinetic data are presented which suggest that a beta-ribofuranosyl moiety may be attached to the adenine. Glucose and allomucic acid have been positively identified as hydrolysis fragments from the exotoxin. These results are discussed and compared with the results of others on similar (or possibly identical) compounds.</description><subject>Adenine - analysis</subject><subject>Bacillus - analysis</subject><subject>Biological Assay</subject><subject>Insecta</subject><subject>Kinetics</subject><subject>Molecular Weight</subject><subject>Phosphates - analysis</subject><subject>Ribose - analysis</subject><subject>Spectrum Analysis</subject><subject>Toxins, Biological - analysis</subject><subject>Toxins, Biological - isolation & purification</subject><issn>0264-6021</issn><issn>0306-3283</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1969</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE9rwzAMxc3Y6Lpuh32Aga87ZLNiO24ug7bsHxR26T0ojtO6JHaw09F9-6W0lO0kJD39xHuE3AN7AibS53ILIJhQ6oKMQSiWTFU6vSRjlmYiyVgK1-Qmxi1jBxUbkZGcpizjckz0jHa7YGursbfeUXQVjb41tAu-M6G3JlJfD2NqXTS6t9pW2FCz973fW0fr4Fs6R22bZhdpvxlYbm2NizbSuQmNdSbckqsam2juTnVCVm-vq8VHsvx6_1zMlokWPOsTLgDQIKLkHGQugWFVosx1NrgRJYLKMedi6GVdlYNtzJWSqhSiBgDDJ-TliO12ZWsqbVwfsCm6YFsMP4VHW_zfOLsp1v67AJiKHPIB8HgE6OBjDKY-3wIrDjkX55wH7cPfZ2flKVj-C6fQetc</recordid><startdate>19690901</startdate><enddate>19690901</enddate><creator>Bond, R P</creator><creator>Boyce, C B</creator><creator>French, S J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19690901</creationdate><title>A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner</title><author>Bond, R P ; Boyce, C B ; French, S J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-3411aeaaa533159510adba59c68724ba179a9349c65fdb042a97757b44f111e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1969</creationdate><topic>Adenine - analysis</topic><topic>Bacillus - analysis</topic><topic>Biological Assay</topic><topic>Insecta</topic><topic>Kinetics</topic><topic>Molecular Weight</topic><topic>Phosphates - analysis</topic><topic>Ribose - analysis</topic><topic>Spectrum Analysis</topic><topic>Toxins, Biological - analysis</topic><topic>Toxins, Biological - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bond, R P</creatorcontrib><creatorcontrib>Boyce, C B</creatorcontrib><creatorcontrib>French, S J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bond, R P</au><au>Boyce, C B</au><au>French, S J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1969-09-01</date><risdate>1969</risdate><volume>114</volume><issue>3</issue><spage>477</spage><epage>488</epage><pages>477-488</pages><issn>0264-6021</issn><issn>0306-3283</issn><eissn>1470-8728</eissn><abstract>An insecticidal exotoxin from Bacillus thuringiensis var. thuringiensis (Berliner) has been purified. The efficiency of each stage of the purification has been ascertained and the yield of toxic material estimated by means of a quantitative bioassay. It is shown that the exotoxin is an adenine derivative substituted at position 9 and having a molecular weight of approximately 825. It can be dephosphorylated enzymically or chemically under conditions that define the exotoxin as a phosphomonoester. This results in loss of toxicity, both to insects and to mice. Spectroscopic and kinetic data are presented which suggest that a beta-ribofuranosyl moiety may be attached to the adenine. Glucose and allomucic acid have been positively identified as hydrolysis fragments from the exotoxin. These results are discussed and compared with the results of others on similar (or possibly identical) compounds.</abstract><cop>England</cop><pmid>5820635</pmid><doi>10.1042/bj1140477</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical journal, 1969-09, Vol.114 (3), p.477-488 |
| issn | 0264-6021 0306-3283 1470-8728 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adenine - analysis Bacillus - analysis Biological Assay Insecta Kinetics Molecular Weight Phosphates - analysis Ribose - analysis Spectrum Analysis Toxins, Biological - analysis Toxins, Biological - isolation & purification |
| title | A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner |
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