A simple sensitive method, applicable to small polypeptides, for the determination of proteins in polyacrylamide gels after isoelectric focusing

A simple method is described for the determination of polypeptides and proteins in polyacrylamide gels after isoelectric focusing. Precipitates formed in trichloroacetic acid, under controlled conditions, are quantified densitometrically by measuring the proportion of light scattered. The procedure...

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Veröffentlicht in:	Biochemical journal 1978-04, Vol.171 (1), p.79-82
Hauptverfasser:	Storring, P L, Tiplady, R J
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemical Precipitation Densitometry Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Methods Peptides - analysis Proteins - analysis Trichloroacetic Acid
Online-Zugang:	Volltext
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