Development of the lung liquid reabsorptive mechanism in fetal sheep: synergism of triiodothyronine and hydrocortisone
1. Thyroidectomy was performed on twelve fetal sheep between 111 and 115 days gestation. Measurement of fetal lung liquid secretion and absorption rates (Jv) were made at rest and during short (45 min) and long (5 h) infusions of adrenaline (0.5 micrograms/min) in a total of thirty-seven experiments...
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| Veröffentlicht in: | The Journal of physiology 1991-02, Vol.433 (1), p.435-449 |
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| creator | Barker, P M Walters, D V Markiewicz, M Strang, L B |
| description | 1. Thyroidectomy was performed on twelve fetal sheep between 111 and 115 days gestation. Measurement of fetal lung liquid
secretion and absorption rates (Jv) were made at rest and during short (45 min) and long (5 h) infusions of adrenaline (0.5
micrograms/min) in a total of thirty-seven experiments, some in the absence of triiodothyronine (T3) and hydrocortisone and
some at set times after the administration of the two hormones. 2. T3 was given either as an I.V. infusion (60 micrograms/24
h) or as a bolus of 30 micrograms; hydrocortisone was given as an infusion of 10 mg/24 h. Both hormones were administered
together. 3. Before T3 and hydrocortisone were given short infusions of adrenaline had no effect on Jv but 4 h after exposure
to the hormones secretion rate was reduced to near zero (Jv = -0.5 +/- 1.6 ml/h, n = 4) by adrenaline; after 24 h of hormone
exposure, absorption of fetal lung liquid was produced by adrenaline (Jv = -3.6 +/- 2.2 ml/h, n = 4) which was even greater
after 72 h, (Jv = -11.2 +/- 2.2 ml/h, n = 4). 4. During long infusions of adrenaline when T3 and hydrocortisone were given
at the start of the experiment, an effect on lung liquid secretion was evident at 2 h and absorption was produced at 4 h (Jv
= -4.2 +/- 2.5 ml/h, n = 3). The effect was significantly different from control long infusions of adrenaline performed the
previous day in the absence of hormones. 5. After 24 or 48 h of stopping T3 and hydrocortisone administration, adrenaline
no longer produced absorption of lung liquid, indicating that the effect of the two hormones was reversible within 24-48 h.
6. The protein synthesis inhibitor cycloheximide put into lung liquid (4 x 10(-5) to 3 x 10(-4) M) blunted the effect of the
hormones at 4 h and prevented absorption of lung liquid at 24 h. Jv during adrenaline was -3.6 +/- 1.5 ml/h in control experiments
but was +3.3 +/- 0.9 ml/h after cycloheximide, n = 4, P < 0.01. This indicated that the two hormones produced their effect
through protein synthesis. |
| doi_str_mv | 10.1113/jphysiol.1991.sp018436 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1181381</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>TJP19914331435</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5305-28cd1e94b6d8465a18b057291714208a621295a3bda6add8ad73a832053a2033</originalsourceid><addsrcrecordid>eNqNkc1u1DAUhS0EKsPAI4C8Qawy-MZxErNAKuWnrSrBYvaWE99MXCV2sDNT5e1JlJbSHZItSz7fOVf2IeQdsB0A8I-3QztF67sdSAm7ODAoM54_IxvIcpkUheTPyYaxNE14IeAleRXjLWPAmZRn5GyGQQrYkNNXPGHnhx7dSH1DxxZpd3QH2tnfR2toQF1FH4bRnpD2WLfa2dhT62iDo-5obBGHTzRODsNhUZaMYK03fmyn4J11SLUztJ1M8LUPo43e4WvyotFdxDf355bsv3_bX1wmNz9_XF2c3yS14EwkaVkbQJlVuSmzXGgoKyaKVEIBWcpKnaeQSqF5ZXSujSm1KbguecoE1ynjfEs-r7HDserR1PMjg-7UEGyvw6S8tuqp4myrDv6kAErg89qSfA2og48xYPPXC0wtPaiHHtTSg3roYTa-_Xfyo239-Fl_f6_rWOuuCdrVNj5isshzIYuZ-7Jyd7bD6T-nq_31r-Ui43zeYg75sIa09tDe2YBqtUVfWxwnNXMK1EL-AehWupk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Development of the lung liquid reabsorptive mechanism in fetal sheep: synergism of triiodothyronine and hydrocortisone</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>IngentaConnect Free/Open Access Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Barker, P M ; Walters, D V ; Markiewicz, M ; Strang, L B</creator><creatorcontrib>Barker, P M ; Walters, D V ; Markiewicz, M ; Strang, L B</creatorcontrib><description>1. Thyroidectomy was performed on twelve fetal sheep between 111 and 115 days gestation. Measurement of fetal lung liquid
secretion and absorption rates (Jv) were made at rest and during short (45 min) and long (5 h) infusions of adrenaline (0.5
micrograms/min) in a total of thirty-seven experiments, some in the absence of triiodothyronine (T3) and hydrocortisone and
some at set times after the administration of the two hormones. 2. T3 was given either as an I.V. infusion (60 micrograms/24
h) or as a bolus of 30 micrograms; hydrocortisone was given as an infusion of 10 mg/24 h. Both hormones were administered
together. 3. Before T3 and hydrocortisone were given short infusions of adrenaline had no effect on Jv but 4 h after exposure
to the hormones secretion rate was reduced to near zero (Jv = -0.5 +/- 1.6 ml/h, n = 4) by adrenaline; after 24 h of hormone
exposure, absorption of fetal lung liquid was produced by adrenaline (Jv = -3.6 +/- 2.2 ml/h, n = 4) which was even greater
after 72 h, (Jv = -11.2 +/- 2.2 ml/h, n = 4). 4. During long infusions of adrenaline when T3 and hydrocortisone were given
at the start of the experiment, an effect on lung liquid secretion was evident at 2 h and absorption was produced at 4 h (Jv
= -4.2 +/- 2.5 ml/h, n = 3). The effect was significantly different from control long infusions of adrenaline performed the
previous day in the absence of hormones. 5. After 24 or 48 h of stopping T3 and hydrocortisone administration, adrenaline
no longer produced absorption of lung liquid, indicating that the effect of the two hormones was reversible within 24-48 h.
6. The protein synthesis inhibitor cycloheximide put into lung liquid (4 x 10(-5) to 3 x 10(-4) M) blunted the effect of the
hormones at 4 h and prevented absorption of lung liquid at 24 h. Jv during adrenaline was -3.6 +/- 1.5 ml/h in control experiments
but was +3.3 +/- 0.9 ml/h after cycloheximide, n = 4, P < 0.01. This indicated that the two hormones produced their effect
through protein synthesis.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1991.sp018436</identifier><identifier>PMID: 1841951</identifier><identifier>CODEN: JPHYA7</identifier><language>eng</language><publisher>Oxford: The Physiological Society</publisher><subject>Absorption - drug effects ; Animals ; Biological and medical sciences ; Body Fluids - physiology ; Cycloheximide - pharmacology ; Drug Synergism ; Embryology: invertebrates and vertebrates. Teratology ; Epinephrine - administration & dosage ; Female ; Fetal Organ Maturity - drug effects ; Fetal Organ Maturity - physiology ; Fetus - drug effects ; Fetus - physiology ; Fundamental and applied biological sciences. Psychology ; Hydrocortisone - administration & dosage ; Hydrocortisone - blood ; Ion Transport - drug effects ; Lung - drug effects ; Lung - embryology ; Lung - physiology ; Organogenesis. Fetal development ; Organogenesis. Physiological fonctions ; Pregnancy ; Sheep ; Thyroidectomy ; Triiodothyronine - administration & dosage ; Triiodothyronine - blood</subject><ispartof>The Journal of physiology, 1991-02, Vol.433 (1), p.435-449</ispartof><rights>1991 The Physiological Society</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5305-28cd1e94b6d8465a18b057291714208a621295a3bda6add8ad73a832053a2033</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1181381/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1181381/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,1412,27905,27906,45555,45556,53772,53774</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19766597$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1841951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barker, P M</creatorcontrib><creatorcontrib>Walters, D V</creatorcontrib><creatorcontrib>Markiewicz, M</creatorcontrib><creatorcontrib>Strang, L B</creatorcontrib><title>Development of the lung liquid reabsorptive mechanism in fetal sheep: synergism of triiodothyronine and hydrocortisone</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. Thyroidectomy was performed on twelve fetal sheep between 111 and 115 days gestation. Measurement of fetal lung liquid
secretion and absorption rates (Jv) were made at rest and during short (45 min) and long (5 h) infusions of adrenaline (0.5
micrograms/min) in a total of thirty-seven experiments, some in the absence of triiodothyronine (T3) and hydrocortisone and
some at set times after the administration of the two hormones. 2. T3 was given either as an I.V. infusion (60 micrograms/24
h) or as a bolus of 30 micrograms; hydrocortisone was given as an infusion of 10 mg/24 h. Both hormones were administered
together. 3. Before T3 and hydrocortisone were given short infusions of adrenaline had no effect on Jv but 4 h after exposure
to the hormones secretion rate was reduced to near zero (Jv = -0.5 +/- 1.6 ml/h, n = 4) by adrenaline; after 24 h of hormone
exposure, absorption of fetal lung liquid was produced by adrenaline (Jv = -3.6 +/- 2.2 ml/h, n = 4) which was even greater
after 72 h, (Jv = -11.2 +/- 2.2 ml/h, n = 4). 4. During long infusions of adrenaline when T3 and hydrocortisone were given
at the start of the experiment, an effect on lung liquid secretion was evident at 2 h and absorption was produced at 4 h (Jv
= -4.2 +/- 2.5 ml/h, n = 3). The effect was significantly different from control long infusions of adrenaline performed the
previous day in the absence of hormones. 5. After 24 or 48 h of stopping T3 and hydrocortisone administration, adrenaline
no longer produced absorption of lung liquid, indicating that the effect of the two hormones was reversible within 24-48 h.
6. The protein synthesis inhibitor cycloheximide put into lung liquid (4 x 10(-5) to 3 x 10(-4) M) blunted the effect of the
hormones at 4 h and prevented absorption of lung liquid at 24 h. Jv during adrenaline was -3.6 +/- 1.5 ml/h in control experiments
but was +3.3 +/- 0.9 ml/h after cycloheximide, n = 4, P < 0.01. This indicated that the two hormones produced their effect
through protein synthesis.</description><subject>Absorption - drug effects</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Body Fluids - physiology</subject><subject>Cycloheximide - pharmacology</subject><subject>Drug Synergism</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Epinephrine - administration & dosage</subject><subject>Female</subject><subject>Fetal Organ Maturity - drug effects</subject><subject>Fetal Organ Maturity - physiology</subject><subject>Fetus - drug effects</subject><subject>Fetus - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrocortisone - administration & dosage</subject><subject>Hydrocortisone - blood</subject><subject>Ion Transport - drug effects</subject><subject>Lung - drug effects</subject><subject>Lung - embryology</subject><subject>Lung - physiology</subject><subject>Organogenesis. Fetal development</subject><subject>Organogenesis. Physiological fonctions</subject><subject>Pregnancy</subject><subject>Sheep</subject><subject>Thyroidectomy</subject><subject>Triiodothyronine - administration & dosage</subject><subject>Triiodothyronine - blood</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EKsPAI4C8Qawy-MZxErNAKuWnrSrBYvaWE99MXCV2sDNT5e1JlJbSHZItSz7fOVf2IeQdsB0A8I-3QztF67sdSAm7ODAoM54_IxvIcpkUheTPyYaxNE14IeAleRXjLWPAmZRn5GyGQQrYkNNXPGHnhx7dSH1DxxZpd3QH2tnfR2toQF1FH4bRnpD2WLfa2dhT62iDo-5obBGHTzRODsNhUZaMYK03fmyn4J11SLUztJ1M8LUPo43e4WvyotFdxDf355bsv3_bX1wmNz9_XF2c3yS14EwkaVkbQJlVuSmzXGgoKyaKVEIBWcpKnaeQSqF5ZXSujSm1KbguecoE1ynjfEs-r7HDserR1PMjg-7UEGyvw6S8tuqp4myrDv6kAErg89qSfA2og48xYPPXC0wtPaiHHtTSg3roYTa-_Xfyo239-Fl_f6_rWOuuCdrVNj5isshzIYuZ-7Jyd7bD6T-nq_31r-Ui43zeYg75sIa09tDe2YBqtUVfWxwnNXMK1EL-AehWupk</recordid><startdate>19910201</startdate><enddate>19910201</enddate><creator>Barker, P M</creator><creator>Walters, D V</creator><creator>Markiewicz, M</creator><creator>Strang, L B</creator><general>The Physiological Society</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19910201</creationdate><title>Development of the lung liquid reabsorptive mechanism in fetal sheep: synergism of triiodothyronine and hydrocortisone</title><author>Barker, P M ; Walters, D V ; Markiewicz, M ; Strang, L B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5305-28cd1e94b6d8465a18b057291714208a621295a3bda6add8ad73a832053a2033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Absorption - drug effects</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Body Fluids - physiology</topic><topic>Cycloheximide - pharmacology</topic><topic>Drug Synergism</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Epinephrine - administration & dosage</topic><topic>Female</topic><topic>Fetal Organ Maturity - drug effects</topic><topic>Fetal Organ Maturity - physiology</topic><topic>Fetus - drug effects</topic><topic>Fetus - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrocortisone - administration & dosage</topic><topic>Hydrocortisone - blood</topic><topic>Ion Transport - drug effects</topic><topic>Lung - drug effects</topic><topic>Lung - embryology</topic><topic>Lung - physiology</topic><topic>Organogenesis. Fetal development</topic><topic>Organogenesis. Physiological fonctions</topic><topic>Pregnancy</topic><topic>Sheep</topic><topic>Thyroidectomy</topic><topic>Triiodothyronine - administration & dosage</topic><topic>Triiodothyronine - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barker, P M</creatorcontrib><creatorcontrib>Walters, D V</creatorcontrib><creatorcontrib>Markiewicz, M</creatorcontrib><creatorcontrib>Strang, L B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barker, P M</au><au>Walters, D V</au><au>Markiewicz, M</au><au>Strang, L B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of the lung liquid reabsorptive mechanism in fetal sheep: synergism of triiodothyronine and hydrocortisone</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1991-02-01</date><risdate>1991</risdate><volume>433</volume><issue>1</issue><spage>435</spage><epage>449</epage><pages>435-449</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. Thyroidectomy was performed on twelve fetal sheep between 111 and 115 days gestation. Measurement of fetal lung liquid
secretion and absorption rates (Jv) were made at rest and during short (45 min) and long (5 h) infusions of adrenaline (0.5
micrograms/min) in a total of thirty-seven experiments, some in the absence of triiodothyronine (T3) and hydrocortisone and
some at set times after the administration of the two hormones. 2. T3 was given either as an I.V. infusion (60 micrograms/24
h) or as a bolus of 30 micrograms; hydrocortisone was given as an infusion of 10 mg/24 h. Both hormones were administered
together. 3. Before T3 and hydrocortisone were given short infusions of adrenaline had no effect on Jv but 4 h after exposure
to the hormones secretion rate was reduced to near zero (Jv = -0.5 +/- 1.6 ml/h, n = 4) by adrenaline; after 24 h of hormone
exposure, absorption of fetal lung liquid was produced by adrenaline (Jv = -3.6 +/- 2.2 ml/h, n = 4) which was even greater
after 72 h, (Jv = -11.2 +/- 2.2 ml/h, n = 4). 4. During long infusions of adrenaline when T3 and hydrocortisone were given
at the start of the experiment, an effect on lung liquid secretion was evident at 2 h and absorption was produced at 4 h (Jv
= -4.2 +/- 2.5 ml/h, n = 3). The effect was significantly different from control long infusions of adrenaline performed the
previous day in the absence of hormones. 5. After 24 or 48 h of stopping T3 and hydrocortisone administration, adrenaline
no longer produced absorption of lung liquid, indicating that the effect of the two hormones was reversible within 24-48 h.
6. The protein synthesis inhibitor cycloheximide put into lung liquid (4 x 10(-5) to 3 x 10(-4) M) blunted the effect of the
hormones at 4 h and prevented absorption of lung liquid at 24 h. Jv during adrenaline was -3.6 +/- 1.5 ml/h in control experiments
but was +3.3 +/- 0.9 ml/h after cycloheximide, n = 4, P < 0.01. This indicated that the two hormones produced their effect
through protein synthesis.</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>1841951</pmid><doi>10.1113/jphysiol.1991.sp018436</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of physiology, 1991-02, Vol.433 (1), p.435-449 |
| issn | 0022-3751 1469-7793 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1181381 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; IngentaConnect Free/Open Access Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Absorption - drug effects Animals Biological and medical sciences Body Fluids - physiology Cycloheximide - pharmacology Drug Synergism Embryology: invertebrates and vertebrates. Teratology Epinephrine - administration & dosage Female Fetal Organ Maturity - drug effects Fetal Organ Maturity - physiology Fetus - drug effects Fetus - physiology Fundamental and applied biological sciences. Psychology Hydrocortisone - administration & dosage Hydrocortisone - blood Ion Transport - drug effects Lung - drug effects Lung - embryology Lung - physiology Organogenesis. Fetal development Organogenesis. Physiological fonctions Pregnancy Sheep Thyroidectomy Triiodothyronine - administration & dosage Triiodothyronine - blood |
| title | Development of the lung liquid reabsorptive mechanism in fetal sheep: synergism of triiodothyronine and hydrocortisone |
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