Electrophysiological characterization of ionic transport by the retinal exchanger expressed in human embryonic kidney cells
The retinal Na+:Ca2+, K+exchanger cDNA was transiently expressed in human embryonic kidney (HEK 293) cells by transfection with plasmid DNA. The correct targeting of the expressed protein to the plasma membrane was confirmed by immunocytochemistry. The reverse exchange offrent (Ca2+ imported per Na+...
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| Veröffentlicht in: | Biophysical journal 1997-07, Vol.73 (1), p.45-51 |
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| creator | Navanglone, A. Rispoli, G. Gabellini, N. Carafoli, E. |
| description | The retinal Na+:Ca2+, K+exchanger cDNA was transiently expressed in human embryonic kidney (HEK 293) cells by transfection with plasmid DNA. The correct targeting of the expressed protein to the plasma membrane was confirmed by immunocytochemistry. The reverse exchange offrent (Ca2+ imported per Na+ extruded) was measured in whole-cell voltage-clamp experiments after intracellular perfusion with Na+ (Na+i, 128 mM) and extracellular perfusion with Ca2+ (Ca2o+, 1 mM) and Ko+ (20 mM). As expected, the exchange current was suppressed by removing Ca2o+. Surprisingly, however, it was also abolished by increasing Na+o to almost abolish the Na+ gradient, and it was almost unaffected by the removal of Ko+. Apparently, then, at variance with the exchanger in the rod outer segment, the retinal exchanger expressed in 293 cells acts essentially as a Na+:Ca2+ exchanger and does not require K+ for its electrogenic activity. |
| doi_str_mv | 10.1016/S0006-3495(97)78046-9 |
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Apparently, then, at variance with the exchanger in the rod outer segment, the retinal exchanger expressed in 293 cells acts essentially as a Na+:Ca2+ exchanger and does not require K+ for its electrogenic activity.</description><subject>Calcium - metabolism</subject><subject>Carrier Proteins - biosynthesis</subject><subject>Carrier Proteins - physiology</subject><subject>Cell Line</subject><subject>Cell Membrane - physiology</subject><subject>DNA, Complementary</subject><subject>Embryo, Mammalian</subject><subject>Humans</subject><subject>Kidney</subject><subject>Membrane Potentials</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium - metabolism</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Retina - physiology</subject><subject>Sodium - metabolism</subject><subject>Sodium-Calcium Exchanger</subject><subject>Time Factors</subject><subject>Transfection</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcmO1DAQtRBoaAY-YSSfEBwCZWdxfAGNRsMijcQBOFuOXekYEjvY6RGBn8e9qAUnTmXpLVXPj5ArBq8YsOb1ZwBoirKS9QspXooWqqaQD8iG1RUvANrmIdmcKY_Jk5S-ATBeA7sgF5JJKQRsyO_bEc0SwzysyYUxbJ3RIzWDjtosGN0vvbjgaehpHs7QJWqf5hAX2q10GZBGXJzPEvyZRX6LMb_miCmhpc7TYTdpT3Hq4nrQf3fW40oNjmN6Sh71ekz47DQvydd3t19uPhR3n95_vLm-K0zV8KXg1sq2s6bqLOtFJ2StZd9WBiRnNW91A6LtWQfCoABRl33Z81bKuhJNlfP35SV5c_Sdd92E1qDPKUY1RzfpuKqgnfoX8W5Q23CvGGtBgsgGz08GMfzYYVrU5NI-gvYYdkmJTBK85JlYH4kmhpQi9uclDNS-NXVoTe0rUVKoQ2tKZt3V3xeeVaeaMv72iGP-pnuHUSXj0Bu0Lub6lA3uPxv-AI7Aq3U</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>Navanglone, A.</creator><creator>Rispoli, G.</creator><creator>Gabellini, N.</creator><creator>Carafoli, E.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970701</creationdate><title>Electrophysiological characterization of ionic transport by the retinal exchanger expressed in human embryonic kidney cells</title><author>Navanglone, A. ; Rispoli, G. ; Gabellini, N. ; Carafoli, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-2dd98bdc4bd1f7b795a9f84c0921528a6078f1b07ce70753f3f289954764006f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Calcium - metabolism</topic><topic>Carrier Proteins - biosynthesis</topic><topic>Carrier Proteins - physiology</topic><topic>Cell Line</topic><topic>Cell Membrane - physiology</topic><topic>DNA, Complementary</topic><topic>Embryo, Mammalian</topic><topic>Humans</topic><topic>Kidney</topic><topic>Membrane Potentials</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium - metabolism</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Retina - physiology</topic><topic>Sodium - metabolism</topic><topic>Sodium-Calcium Exchanger</topic><topic>Time Factors</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Navanglone, A.</creatorcontrib><creatorcontrib>Rispoli, G.</creatorcontrib><creatorcontrib>Gabellini, N.</creatorcontrib><creatorcontrib>Carafoli, E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Navanglone, A.</au><au>Rispoli, G.</au><au>Gabellini, N.</au><au>Carafoli, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrophysiological characterization of ionic transport by the retinal exchanger expressed in human embryonic kidney cells</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>73</volume><issue>1</issue><spage>45</spage><epage>51</epage><pages>45-51</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>The retinal Na+:Ca2+, K+exchanger cDNA was transiently expressed in human embryonic kidney (HEK 293) cells by transfection with plasmid DNA. 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| ispartof | Biophysical journal, 1997-07, Vol.73 (1), p.45-51 |
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| source | MEDLINE; Cell Press Free Archives; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Calcium - metabolism Carrier Proteins - biosynthesis Carrier Proteins - physiology Cell Line Cell Membrane - physiology DNA, Complementary Embryo, Mammalian Humans Kidney Membrane Potentials Patch-Clamp Techniques Potassium - metabolism Recombinant Proteins - biosynthesis Retina - physiology Sodium - metabolism Sodium-Calcium Exchanger Time Factors Transfection |
| title | Electrophysiological characterization of ionic transport by the retinal exchanger expressed in human embryonic kidney cells |
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