Effect of Ag+ on membrane permeability of perfused Helix pomatia neurons

1. Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. 2. Ions of Ag+ (1-100 microM) introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible when the concentration of Ag+ was less than...

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Veröffentlicht in:The Journal of physiology 1991-10, Vol.442 (1), p.1-13
Hauptverfasser: Györi, J, Kiss, T, Shcherbatko, A D, Belan, P V, Tepikin, A V, Osipenko, O N, Salánki, J
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container_end_page 13
container_issue 1
container_start_page 1
container_title The Journal of physiology
container_volume 442
creator Györi, J
Kiss, T
Shcherbatko, A D
Belan, P V
Tepikin, A V
Osipenko, O N
Salánki, J
description 1. Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. 2. Ions of Ag+ (1-100 microM) introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible when the concentration of Ag+ was less than 75 microM or the time of application was shorter than 10 min. 3. IAg was observed both in the presence and absence of Na+ ions in the extracellular saline. It could also be activated when Cs+ ions were substituted for Na+ ions. 4. The current-voltage characteristics were linear in the voltage range -100 to 0 mV. The reversal potential in control saline was an average of 1.19 +/- 5.1 mV. 5. The application of Ag+ ions induces an elevation of intracellular free Ca2+ concentration by 10-20 times in both Ca(2+)-containing and Ca(2+)-free extracellular salines, as revealed by Fura-2 measurements. 6. Agents that increase the intracellular free Ca2+ concentration ([Ca2+]i), like thymol, caffeine and dinitrophenol, increased the amplitude of IAg. The effect was additive. Ruthenium Red, which blocks the release of Ca2+ from intracellular stores, decreased the Ag+ effect. 7. It is concluded that extracellularly applied Ag+ ions increase the cytoplasmic free Ca2+ concentration, which in turn activates non-specific cationic channels. 8. Ag+ ions in 1-10 microM concentration were able to decrease the voltage-activated Ca2+ current amplitude. This decrease, however, was due to the increase of [Ca2+]i which caused Ca(2+)-dependent inactivation.
doi_str_mv 10.1113/jphysiol.1991.sp018778
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Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. 2. Ions of Ag+ (1-100 microM) introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible when the concentration of Ag+ was less than 75 microM or the time of application was shorter than 10 min. 3. IAg was observed both in the presence and absence of Na+ ions in the extracellular saline. It could also be activated when Cs+ ions were substituted for Na+ ions. 4. The current-voltage characteristics were linear in the voltage range -100 to 0 mV. The reversal potential in control saline was an average of 1.19 +/- 5.1 mV. 5. The application of Ag+ ions induces an elevation of intracellular free Ca2+ concentration by 10-20 times in both Ca(2+)-containing and Ca(2+)-free extracellular salines, as revealed by Fura-2 measurements. 6. 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Physiology. Immunology ; Biological and medical sciences ; Calcium - metabolism ; Calcium Channels - drug effects ; Cell Membrane Permeability - drug effects ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. Psychology ; Helix (Snails) ; Helix pomatia ; Invertebrates ; Mollusca ; Neural Conduction - drug effects ; Neurons - drug effects ; Neurons - metabolism ; Physiology. 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Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. 2. Ions of Ag+ (1-100 microM) introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible when the concentration of Ag+ was less than 75 microM or the time of application was shorter than 10 min. 3. IAg was observed both in the presence and absence of Na+ ions in the extracellular saline. It could also be activated when Cs+ ions were substituted for Na+ ions. 4. The current-voltage characteristics were linear in the voltage range -100 to 0 mV. The reversal potential in control saline was an average of 1.19 +/- 5.1 mV. 5. The application of Ag+ ions induces an elevation of intracellular free Ca2+ concentration by 10-20 times in both Ca(2+)-containing and Ca(2+)-free extracellular salines, as revealed by Fura-2 measurements. 6. Agents that increase the intracellular free Ca2+ concentration ([Ca2+]i), like thymol, caffeine and dinitrophenol, increased the amplitude of IAg. The effect was additive. Ruthenium Red, which blocks the release of Ca2+ from intracellular stores, decreased the Ag+ effect. 7. It is concluded that extracellularly applied Ag+ ions increase the cytoplasmic free Ca2+ concentration, which in turn activates non-specific cationic channels. 8. Ag+ ions in 1-10 microM concentration were able to decrease the voltage-activated Ca2+ current amplitude. This decrease, however, was due to the increase of [Ca2+]i which caused Ca(2+)-dependent inactivation.</description><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Calcium Channels - drug effects</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Helix (Snails)</subject><subject>Helix pomatia</subject><subject>Invertebrates</subject><subject>Mollusca</subject><subject>Neural Conduction - drug effects</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Physiology. 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Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Calcium Channels - drug effects</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Helix (Snails)</topic><topic>Helix pomatia</topic><topic>Invertebrates</topic><topic>Mollusca</topic><topic>Neural Conduction - drug effects</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Physiology. Development</topic><topic>Silver - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Györi, J</creatorcontrib><creatorcontrib>Kiss, T</creatorcontrib><creatorcontrib>Shcherbatko, A D</creatorcontrib><creatorcontrib>Belan, P V</creatorcontrib><creatorcontrib>Tepikin, A V</creatorcontrib><creatorcontrib>Osipenko, O N</creatorcontrib><creatorcontrib>Salánki, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Györi, J</au><au>Kiss, T</au><au>Shcherbatko, A D</au><au>Belan, P V</au><au>Tepikin, A V</au><au>Osipenko, O N</au><au>Salánki, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Ag+ on membrane permeability of perfused Helix pomatia neurons</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1991-10-01</date><risdate>1991</risdate><volume>442</volume><issue>1</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. 2. Ions of Ag+ (1-100 microM) introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible when the concentration of Ag+ was less than 75 microM or the time of application was shorter than 10 min. 3. IAg was observed both in the presence and absence of Na+ ions in the extracellular saline. It could also be activated when Cs+ ions were substituted for Na+ ions. 4. The current-voltage characteristics were linear in the voltage range -100 to 0 mV. The reversal potential in control saline was an average of 1.19 +/- 5.1 mV. 5. The application of Ag+ ions induces an elevation of intracellular free Ca2+ concentration by 10-20 times in both Ca(2+)-containing and Ca(2+)-free extracellular salines, as revealed by Fura-2 measurements. 6. Agents that increase the intracellular free Ca2+ concentration ([Ca2+]i), like thymol, caffeine and dinitrophenol, increased the amplitude of IAg. The effect was additive. Ruthenium Red, which blocks the release of Ca2+ from intracellular stores, decreased the Ag+ effect. 7. It is concluded that extracellularly applied Ag+ ions increase the cytoplasmic free Ca2+ concentration, which in turn activates non-specific cationic channels. 8. Ag+ ions in 1-10 microM concentration were able to decrease the voltage-activated Ca2+ current amplitude. This decrease, however, was due to the increase of [Ca2+]i which caused Ca(2+)-dependent inactivation.</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>1665853</pmid><doi>10.1113/jphysiol.1991.sp018778</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; PubMed Central; Alma/SFX Local Collection
subjects Animals
Biochemistry. Physiology. Immunology
Biological and medical sciences
Calcium - metabolism
Calcium Channels - drug effects
Cell Membrane Permeability - drug effects
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
Helix (Snails)
Helix pomatia
Invertebrates
Mollusca
Neural Conduction - drug effects
Neurons - drug effects
Neurons - metabolism
Physiology. Development
Silver - metabolism
title Effect of Ag+ on membrane permeability of perfused Helix pomatia neurons
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