Effect of Ag+ on membrane permeability of perfused Helix pomatia neurons
1. Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. 2. Ions of Ag+ (1-100 microM) introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible when the concentration of Ag+ was less than...
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creator | Györi, J Kiss, T Shcherbatko, A D Belan, P V Tepikin, A V Osipenko, O N Salánki, J |
description | 1. Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. 2. Ions of Ag+ (1-100 microM)
introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible
when the concentration of Ag+ was less than 75 microM or the time of application was shorter than 10 min. 3. IAg was observed
both in the presence and absence of Na+ ions in the extracellular saline. It could also be activated when Cs+ ions were substituted
for Na+ ions. 4. The current-voltage characteristics were linear in the voltage range -100 to 0 mV. The reversal potential
in control saline was an average of 1.19 +/- 5.1 mV. 5. The application of Ag+ ions induces an elevation of intracellular
free Ca2+ concentration by 10-20 times in both Ca(2+)-containing and Ca(2+)-free extracellular salines, as revealed by Fura-2
measurements. 6. Agents that increase the intracellular free Ca2+ concentration ([Ca2+]i), like thymol, caffeine and dinitrophenol,
increased the amplitude of IAg. The effect was additive. Ruthenium Red, which blocks the release of Ca2+ from intracellular
stores, decreased the Ag+ effect. 7. It is concluded that extracellularly applied Ag+ ions increase the cytoplasmic free Ca2+
concentration, which in turn activates non-specific cationic channels. 8. Ag+ ions in 1-10 microM concentration were able
to decrease the voltage-activated Ca2+ current amplitude. This decrease, however, was due to the increase of [Ca2+]i which
caused Ca(2+)-dependent inactivation. |
doi_str_mv | 10.1113/jphysiol.1991.sp018778 |
format | Article |
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introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible
when the concentration of Ag+ was less than 75 microM or the time of application was shorter than 10 min. 3. IAg was observed
both in the presence and absence of Na+ ions in the extracellular saline. It could also be activated when Cs+ ions were substituted
for Na+ ions. 4. The current-voltage characteristics were linear in the voltage range -100 to 0 mV. The reversal potential
in control saline was an average of 1.19 +/- 5.1 mV. 5. The application of Ag+ ions induces an elevation of intracellular
free Ca2+ concentration by 10-20 times in both Ca(2+)-containing and Ca(2+)-free extracellular salines, as revealed by Fura-2
measurements. 6. Agents that increase the intracellular free Ca2+ concentration ([Ca2+]i), like thymol, caffeine and dinitrophenol,
increased the amplitude of IAg. The effect was additive. Ruthenium Red, which blocks the release of Ca2+ from intracellular
stores, decreased the Ag+ effect. 7. It is concluded that extracellularly applied Ag+ ions increase the cytoplasmic free Ca2+
concentration, which in turn activates non-specific cationic channels. 8. Ag+ ions in 1-10 microM concentration were able
to decrease the voltage-activated Ca2+ current amplitude. This decrease, however, was due to the increase of [Ca2+]i which
caused Ca(2+)-dependent inactivation.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1991.sp018778</identifier><identifier>PMID: 1665853</identifier><identifier>CODEN: JPHYA7</identifier><language>eng</language><publisher>Oxford: The Physiological Society</publisher><subject>Animals ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; Calcium - metabolism ; Calcium Channels - drug effects ; Cell Membrane Permeability - drug effects ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. Psychology ; Helix (Snails) ; Helix pomatia ; Invertebrates ; Mollusca ; Neural Conduction - drug effects ; Neurons - drug effects ; Neurons - metabolism ; Physiology. Development ; Silver - metabolism</subject><ispartof>The Journal of physiology, 1991-10, Vol.442 (1), p.1-13</ispartof><rights>1991 The Physiological Society</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5581-aa01ffd70bf2cc87cff3ca7699deec1e6f63c085b47d4a4a36c0b736848dca203</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1179874/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1179874/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,27924,27925,45574,45575,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5009065$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1665853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Györi, J</creatorcontrib><creatorcontrib>Kiss, T</creatorcontrib><creatorcontrib>Shcherbatko, A D</creatorcontrib><creatorcontrib>Belan, P V</creatorcontrib><creatorcontrib>Tepikin, A V</creatorcontrib><creatorcontrib>Osipenko, O N</creatorcontrib><creatorcontrib>Salánki, J</creatorcontrib><title>Effect of Ag+ on membrane permeability of perfused Helix pomatia neurons</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. 2. Ions of Ag+ (1-100 microM)
introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible
when the concentration of Ag+ was less than 75 microM or the time of application was shorter than 10 min. 3. IAg was observed
both in the presence and absence of Na+ ions in the extracellular saline. It could also be activated when Cs+ ions were substituted
for Na+ ions. 4. The current-voltage characteristics were linear in the voltage range -100 to 0 mV. The reversal potential
in control saline was an average of 1.19 +/- 5.1 mV. 5. The application of Ag+ ions induces an elevation of intracellular
free Ca2+ concentration by 10-20 times in both Ca(2+)-containing and Ca(2+)-free extracellular salines, as revealed by Fura-2
measurements. 6. Agents that increase the intracellular free Ca2+ concentration ([Ca2+]i), like thymol, caffeine and dinitrophenol,
increased the amplitude of IAg. The effect was additive. Ruthenium Red, which blocks the release of Ca2+ from intracellular
stores, decreased the Ag+ effect. 7. It is concluded that extracellularly applied Ag+ ions increase the cytoplasmic free Ca2+
concentration, which in turn activates non-specific cationic channels. 8. Ag+ ions in 1-10 microM concentration were able
to decrease the voltage-activated Ca2+ current amplitude. This decrease, however, was due to the increase of [Ca2+]i which
caused Ca(2+)-dependent inactivation.</description><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Calcium Channels - drug effects</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Helix (Snails)</subject><subject>Helix pomatia</subject><subject>Invertebrates</subject><subject>Mollusca</subject><subject>Neural Conduction - drug effects</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Physiology. Development</subject><subject>Silver - metabolism</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EKkPhEUBZAEJCGXzjxD8bRFUVBlQJFmVtOY494yqJUzuh5O1xlGmBDWJl2ee75_roIPQC8BYAyLvr4TBH59stCAHbOGDgjPEHaAMlFTljgjxEG4yLIiesgsfoSYzXGAPBQpygE6C04hXZoN2FtUaPmbfZ2f5t5vusM10dVG-ywYTOqNq1bpwXPd3tFE2T7UzrfmaD79ToVNabKfg-PkWPrGqjeXY8T9H3jxdX57v88uunz-dnl7muKg65UhisbRiubaE1Z9paohWjQjTGaDDUUqIxr-qSNaUqFaEa14xQXvJGqwKTU_R-9R2mujONNv0YVCuH4DoVZumVk38rvTvIvf8hAZjgrEwGr48Gwd9MJo6yc1Gbtk2Z_RQlKyjloigS-OafIDBS8EJUJSSUrqgOPsZg7P1_AMulLnlXl1zqknd1pcHnf6b5Pbb2k_SXR11FrVqbitEu3mMVxgLTKmEfVuzWtWb-z-Xy6su35aEsC1givFotDm5_uHXByHUoeu3MOMtESZBAfgHTBsQf</recordid><startdate>19911001</startdate><enddate>19911001</enddate><creator>Györi, J</creator><creator>Kiss, T</creator><creator>Shcherbatko, A D</creator><creator>Belan, P V</creator><creator>Tepikin, A V</creator><creator>Osipenko, O N</creator><creator>Salánki, J</creator><general>The Physiological Society</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19911001</creationdate><title>Effect of Ag+ on membrane permeability of perfused Helix pomatia neurons</title><author>Györi, J ; Kiss, T ; Shcherbatko, A D ; Belan, P V ; Tepikin, A V ; Osipenko, O N ; Salánki, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5581-aa01ffd70bf2cc87cff3ca7699deec1e6f63c085b47d4a4a36c0b736848dca203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Calcium Channels - drug effects</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Helix (Snails)</topic><topic>Helix pomatia</topic><topic>Invertebrates</topic><topic>Mollusca</topic><topic>Neural Conduction - drug effects</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Physiology. Development</topic><topic>Silver - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Györi, J</creatorcontrib><creatorcontrib>Kiss, T</creatorcontrib><creatorcontrib>Shcherbatko, A D</creatorcontrib><creatorcontrib>Belan, P V</creatorcontrib><creatorcontrib>Tepikin, A V</creatorcontrib><creatorcontrib>Osipenko, O N</creatorcontrib><creatorcontrib>Salánki, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Györi, J</au><au>Kiss, T</au><au>Shcherbatko, A D</au><au>Belan, P V</au><au>Tepikin, A V</au><au>Osipenko, O N</au><au>Salánki, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Ag+ on membrane permeability of perfused Helix pomatia neurons</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1991-10-01</date><risdate>1991</risdate><volume>442</volume><issue>1</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. 2. Ions of Ag+ (1-100 microM)
introduced into the bathing solution activated a steady-state inward current (IAg) in the soma. The effect of Ag+ was reversible
when the concentration of Ag+ was less than 75 microM or the time of application was shorter than 10 min. 3. IAg was observed
both in the presence and absence of Na+ ions in the extracellular saline. It could also be activated when Cs+ ions were substituted
for Na+ ions. 4. The current-voltage characteristics were linear in the voltage range -100 to 0 mV. The reversal potential
in control saline was an average of 1.19 +/- 5.1 mV. 5. The application of Ag+ ions induces an elevation of intracellular
free Ca2+ concentration by 10-20 times in both Ca(2+)-containing and Ca(2+)-free extracellular salines, as revealed by Fura-2
measurements. 6. Agents that increase the intracellular free Ca2+ concentration ([Ca2+]i), like thymol, caffeine and dinitrophenol,
increased the amplitude of IAg. The effect was additive. Ruthenium Red, which blocks the release of Ca2+ from intracellular
stores, decreased the Ag+ effect. 7. It is concluded that extracellularly applied Ag+ ions increase the cytoplasmic free Ca2+
concentration, which in turn activates non-specific cationic channels. 8. Ag+ ions in 1-10 microM concentration were able
to decrease the voltage-activated Ca2+ current amplitude. This decrease, however, was due to the increase of [Ca2+]i which
caused Ca(2+)-dependent inactivation.</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>1665853</pmid><doi>10.1113/jphysiol.1991.sp018778</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; PubMed Central; Alma/SFX Local Collection |
subjects | Animals Biochemistry. Physiology. Immunology Biological and medical sciences Calcium - metabolism Calcium Channels - drug effects Cell Membrane Permeability - drug effects Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Helix (Snails) Helix pomatia Invertebrates Mollusca Neural Conduction - drug effects Neurons - drug effects Neurons - metabolism Physiology. Development Silver - metabolism |
title | Effect of Ag+ on membrane permeability of perfused Helix pomatia neurons |
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