Fractionation of nuclear and cytoplasmic ribonucleic acids from rat tissues on the methylated albumin--kieselguhr column

1. The conformation of RNA was found to affect its behaviour on methylated albumin-kieselguhr chromatography. The less regular the secondary structure of RNA, the more tightly it binds to the methylated albumin-kieselguhr column. 2. The presence of various denaturing agents (such as urea or perchlor...

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Veröffentlicht in:	Biochemical journal 1971-11, Vol.125 (1), p.225-234
Hauptverfasser:	Lichtenstein, A V, Shapot, V S
Format:	Artikel
Sprache:	eng
Schlagworte:	Albumins Animals Cell Nucleus - analysis Centrifugation, Density Gradient Chromatography Cytoplasm - analysis Dactinomycin Female Kidney Liver Male Methods Nucleic Acid Conformation Perchlorates Protein Binding Rats RNA - isolation & purification RNA, Ribosomal - isolation & purification Silicon Dioxide Spleen Temperature Thymus Gland Ultracentrifugation Urea
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