Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases

1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5m-potassium chloride-1m-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-...

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Veröffentlicht in:	Biochemical journal 1971-02, Vol.121 (4), p.635-641
Hauptverfasser:	Louis, B G, Peterkin, P I, Fitt, P S
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylamides Centrifugation, Density Gradient Chromatography, Gel Cytochromes - analysis Dextrans DNA Halobacterium - enzymology Methods Molecular Weight Potassium Chloride Proteins - analysis RNA RNA Nucleotidyltransferases Sodium Chloride Spectrophotometry Ultracentrifugation
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container_title	Biochemical journal
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creator	Louis, B G Peterkin, P I Fitt, P S
description	1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5m-potassium chloride-1m-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-dependent RNA polymerase have been studied by these methods. 2. The RNA-dependent polymerase has also been studied by density-gradient centrifugation in the absence of salt. 3. All three proteins are of unusually low molecular weight compared with similar enzymes from non-halophilic bacteria.
doi_str_mv	10.1042/bj1210635
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The RNA-dependent polymerase has also been studied by density-gradient centrifugation in the absence of salt. 3. All three proteins are of unusually low molecular weight compared with similar enzymes from non-halophilic bacteria.</description><identifier>ISSN: 0264-6021</identifier><identifier>ISSN: 0306-3283</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj1210635</identifier><identifier>PMID: 5114975</identifier><language>eng</language><publisher>England</publisher><subject>Acrylamides ; Centrifugation, Density Gradient ; Chromatography, Gel ; Cytochromes - analysis ; Dextrans ; DNA ; Halobacterium - enzymology ; Methods ; Molecular Weight ; Potassium Chloride ; Proteins - analysis ; RNA ; RNA Nucleotidyltransferases ; Sodium Chloride ; Spectrophotometry ; Ultracentrifugation</subject><ispartof>Biochemical journal, 1971-02, Vol.121 (4), p.635-641</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2855-9477209b417c2c3b9b94f0e4258b24a4f9c093c67b316430a5d2f166c82a83c23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1176640/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1176640/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5114975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Louis, B G</creatorcontrib><creatorcontrib>Peterkin, P I</creatorcontrib><creatorcontrib>Fitt, P S</creatorcontrib><title>Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5m-potassium chloride-1m-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-dependent RNA polymerase have been studied by these methods. 2. The RNA-dependent polymerase has also been studied by density-gradient centrifugation in the absence of salt. 3. All three proteins are of unusually low molecular weight compared with similar enzymes from non-halophilic bacteria.</description><subject>Acrylamides</subject><subject>Centrifugation, Density Gradient</subject><subject>Chromatography, Gel</subject><subject>Cytochromes - analysis</subject><subject>Dextrans</subject><subject>DNA</subject><subject>Halobacterium - enzymology</subject><subject>Methods</subject><subject>Molecular Weight</subject><subject>Potassium Chloride</subject><subject>Proteins - analysis</subject><subject>RNA</subject><subject>RNA Nucleotidyltransferases</subject><subject>Sodium Chloride</subject><subject>Spectrophotometry</subject><subject>Ultracentrifugation</subject><issn>0264-6021</issn><issn>0306-3283</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplUk1v1DAUDAhUlsKBH1DJJyQOKbbjfF2QUAUtUgUXOEe285K4cuzgj9Lw6_HuRquiHmw_vRnNjO2XZe8IviSY0Y_ijlCCq6J8nu0Iq3He1LR5ke0wrVheYUpeZa-9v8OYMMzwWXZWEsLautw9u_gepQYlEZeqR2D-rrPVdlyRHRA8BAcz6BVNXNtlUjrxBJcBnOKX6Bp0PigdHA_KGsRNj3owXoU1Hx3vFZiQy7Q5NcTxyPEhpr7fi4cJULICGTV36A-ocQoH4CZ5bSZxRjIG5aJwqVysXs0-rQ2qB7RM1qflVs09bO72YXVKWPPoTvkBetLtYQGT4oYn0MFnBpdU_Zvs5cC1h7fbeZ79-vrl59VNfvvj-tvV59tc0qYs85bVNcWtYKSWVBaiFS0bMDBaNoIyzoZW4raQVS0KUrEC87KnA6kq2VDeFJIW59mno-4SxQz94dW47hanZu7WznLV_Y8YNXWjve8IqauK4STwfhNw9ncEH7pZeQlacwM2-q5J_10zsid-OBKls947GE4mBHf7WepOs5S4F49TnZjb8BT_AAXBzt0</recordid><startdate>19710201</startdate><enddate>19710201</enddate><creator>Louis, B G</creator><creator>Peterkin, P I</creator><creator>Fitt, P S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19710201</creationdate><title>Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases</title><author>Louis, B G ; Peterkin, P I ; Fitt, P S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2855-9477209b417c2c3b9b94f0e4258b24a4f9c093c67b316430a5d2f166c82a83c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Acrylamides</topic><topic>Centrifugation, Density Gradient</topic><topic>Chromatography, Gel</topic><topic>Cytochromes - analysis</topic><topic>Dextrans</topic><topic>DNA</topic><topic>Halobacterium - enzymology</topic><topic>Methods</topic><topic>Molecular Weight</topic><topic>Potassium Chloride</topic><topic>Proteins - analysis</topic><topic>RNA</topic><topic>RNA Nucleotidyltransferases</topic><topic>Sodium Chloride</topic><topic>Spectrophotometry</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Louis, B G</creatorcontrib><creatorcontrib>Peterkin, P I</creatorcontrib><creatorcontrib>Fitt, P S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Louis, B G</au><au>Peterkin, P I</au><au>Fitt, P S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1971-02-01</date><risdate>1971</risdate><volume>121</volume><issue>4</issue><spage>635</spage><epage>641</epage><pages>635-641</pages><issn>0264-6021</issn><issn>0306-3283</issn><eissn>1470-8728</eissn><abstract>1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5m-potassium chloride-1m-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-dependent RNA polymerase have been studied by these methods. 2. The RNA-dependent polymerase has also been studied by density-gradient centrifugation in the absence of salt. 3. All three proteins are of unusually low molecular weight compared with similar enzymes from non-halophilic bacteria.</abstract><cop>England</cop><pmid>5114975</pmid><doi>10.1042/bj1210635</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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source	MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection
subjects	Acrylamides Centrifugation, Density Gradient Chromatography, Gel Cytochromes - analysis Dextrans DNA Halobacterium - enzymology Methods Molecular Weight Potassium Chloride Proteins - analysis RNA RNA Nucleotidyltransferases Sodium Chloride Spectrophotometry Ultracentrifugation
title	Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases
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