Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars1
We demonstrate the use of laser photoacoustic detection (LPAD) as a highly sensitive method to detect in planta nitric oxide ((*)NO) production from tobacco (Nicotiana tabacum). LPAD calibration against (*)NO gas demonstrated a linear relationship over 2 orders of magnitude with a detection threshol...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2005-07, Vol.138 (3), p.1247-1258 |
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| creator | Mur, Luis A J I Edi Santosa Laarhoven, Lucas J J Holton, Nicholas J |
| description | We demonstrate the use of laser photoacoustic detection (LPAD) as a highly sensitive method to detect in planta nitric oxide ((*)NO) production from tobacco (Nicotiana tabacum). LPAD calibration against (*)NO gas demonstrated a linear relationship over 2 orders of magnitude with a detection threshold of NO(2) + O(2)). The utility of the LPAD method was shown by examination of a nonhost hypersensitive response and a disease induced by Pseudomonas syringae (P. s.) pv phaseolicola and P. s. pv tabaci in tobacco. (*)NO was detected within 40 min of challenge with P. s. pv phaseolicola, some 5 h before the initiation of visible tissue collapse. The wildfire tobacco pathogen P. s. pv tabaci initiated (*)NO generation at 2 h postinfection. The use of (*)NO donors, the scavenger CPTIO ([4-carboxyphenyl]-4,5-dihydro-4,4,5,5-tetramethyl-3-oxide), and the mammalian nitric oxide synthase inhibitor l-NMMA (N(G)-monomethyl-l-arginine) indicated that (*)NO influenced the kinetics of cell death and resistance to both avirulent and virulent bacteria in tobacco. These observations suggest that (*)NO is integral to the elicitation of cell death associated with these two bacterial pathogens in tobacco. |
| doi_str_mv | 10.1104/pp.104.055772 |
| format | Article |
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LPAD calibration against (*)NO gas demonstrated a linear relationship over 2 orders of magnitude with a detection threshold of <20 pmol h(-1) (1 part per billion volume [ppbv]). The specificity of the photoacoustic signal for (*)NO when adding gas or the (*)NO donor, sodium nitroprusside, on injection into plant leaves, was demonstrated by its abolition with O(3) ((*)NO + O(3) --> NO(2) + O(2)). The utility of the LPAD method was shown by examination of a nonhost hypersensitive response and a disease induced by Pseudomonas syringae (P. s.) pv phaseolicola and P. s. pv tabaci in tobacco. (*)NO was detected within 40 min of challenge with P. s. pv phaseolicola, some 5 h before the initiation of visible tissue collapse. The wildfire tobacco pathogen P. s. pv tabaci initiated (*)NO generation at 2 h postinfection. The use of (*)NO donors, the scavenger CPTIO ([4-carboxyphenyl]-4,5-dihydro-4,4,5,5-tetramethyl-3-oxide), and the mammalian nitric oxide synthase inhibitor l-NMMA (N(G)-monomethyl-l-arginine) indicated that (*)NO influenced the kinetics of cell death and resistance to both avirulent and virulent bacteria in tobacco. These observations suggest that (*)NO is integral to the elicitation of cell death associated with these two bacterial pathogens in tobacco.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.104.055772</identifier><identifier>PMID: 16009999</identifier><language>eng</language><publisher>Rockville: American Society of Plant Biologists</publisher><subject>Breakthrough Technologies ; Nitric oxide ; Pathogens ; Tobacco ; Wildfires</subject><ispartof>Plant physiology (Bethesda), 2005-07, Vol.138 (3), p.1247-1258</ispartof><rights>Copyright American Society of Plant Physiologists Jul 2005</rights><rights>Copyright © 2005, American Society of Plant Biologists 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids></links><search><creatorcontrib>Mur, Luis A J</creatorcontrib><creatorcontrib>I Edi Santosa</creatorcontrib><creatorcontrib>Laarhoven, Lucas J J</creatorcontrib><creatorcontrib>Holton, Nicholas J</creatorcontrib><title>Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars1</title><title>Plant physiology (Bethesda)</title><description>We demonstrate the use of laser photoacoustic detection (LPAD) as a highly sensitive method to detect in planta nitric oxide ((*)NO) production from tobacco (Nicotiana tabacum). 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The use of (*)NO donors, the scavenger CPTIO ([4-carboxyphenyl]-4,5-dihydro-4,4,5,5-tetramethyl-3-oxide), and the mammalian nitric oxide synthase inhibitor l-NMMA (N(G)-monomethyl-l-arginine) indicated that (*)NO influenced the kinetics of cell death and resistance to both avirulent and virulent bacteria in tobacco. These observations suggest that (*)NO is integral to the elicitation of cell death associated with these two bacterial pathogens in tobacco.</description><subject>Breakthrough Technologies</subject><subject>Nitric oxide</subject><subject>Pathogens</subject><subject>Tobacco</subject><subject>Wildfires</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpVTstu2zAQJIoGjZv02DuRu12uKFrUpYDh5lHASHwwchVWFGkxkEmFpJzke_KjZdAUSPcyO5gHhpDvwBYArPwxjosMCyZEVRWfyAwEL-aFKOVnMmMs_0zK-pR8jfGBMQYcyi_kFJaM1flm5HWDUQe67X3yqPwUk1X0l05aJesdXQ2Df4rUOrod0CX8IHlDb20K2X73bDv95tn5FpXy1Pi3mHV7uu5xGLTba_pkU09XRxumzBNF19H7f2Qb9dT5g3cYaXwJOYiabjH1_oghwjk5MThE_e0dz8ju6nK3vplv7q5_r1eb-SgLOedGKgDOJQpplm2LLZiq5QJZiQa14HVbYMu6EjjWAo2qjQJWiUIw1TGD_Iz8_Fs7Tu1BdyovCzg0Y7AHDC-NR9v8rzjbN3t_bACqJa9lLrh4Lwj-cdIxNQ9-Ci5PbgqQS4ASav4HMziJEw</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>Mur, Luis A J</creator><creator>I Edi Santosa</creator><creator>Laarhoven, Lucas J J</creator><creator>Holton, Nicholas J</creator><general>American Society of Plant Biologists</general><scope>3V.</scope><scope>4T-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>5PM</scope></search><sort><creationdate>20050701</creationdate><title>Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars1</title><author>Mur, Luis A J ; I Edi Santosa ; Laarhoven, Lucas J J ; Holton, Nicholas J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p828-3f8c11338a58f6bbab1f7b35a04afae539b2ab0d413a95afc9fc1075250cd0fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Breakthrough Technologies</topic><topic>Nitric oxide</topic><topic>Pathogens</topic><topic>Tobacco</topic><topic>Wildfires</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mur, Luis A J</creatorcontrib><creatorcontrib>I Edi Santosa</creatorcontrib><creatorcontrib>Laarhoven, Lucas J J</creatorcontrib><creatorcontrib>Holton, Nicholas J</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mur, Luis A J</au><au>I Edi Santosa</au><au>Laarhoven, Lucas J J</au><au>Holton, Nicholas J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars1</atitle><jtitle>Plant physiology (Bethesda)</jtitle><date>2005-07-01</date><risdate>2005</risdate><volume>138</volume><issue>3</issue><spage>1247</spage><epage>1258</epage><pages>1247-1258</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>We demonstrate the use of laser photoacoustic detection (LPAD) as a highly sensitive method to detect in planta nitric oxide ((*)NO) production from tobacco (Nicotiana tabacum). LPAD calibration against (*)NO gas demonstrated a linear relationship over 2 orders of magnitude with a detection threshold of <20 pmol h(-1) (1 part per billion volume [ppbv]). The specificity of the photoacoustic signal for (*)NO when adding gas or the (*)NO donor, sodium nitroprusside, on injection into plant leaves, was demonstrated by its abolition with O(3) ((*)NO + O(3) --> NO(2) + O(2)). The utility of the LPAD method was shown by examination of a nonhost hypersensitive response and a disease induced by Pseudomonas syringae (P. s.) pv phaseolicola and P. s. pv tabaci in tobacco. (*)NO was detected within 40 min of challenge with P. s. pv phaseolicola, some 5 h before the initiation of visible tissue collapse. The wildfire tobacco pathogen P. s. pv tabaci initiated (*)NO generation at 2 h postinfection. The use of (*)NO donors, the scavenger CPTIO ([4-carboxyphenyl]-4,5-dihydro-4,4,5,5-tetramethyl-3-oxide), and the mammalian nitric oxide synthase inhibitor l-NMMA (N(G)-monomethyl-l-arginine) indicated that (*)NO influenced the kinetics of cell death and resistance to both avirulent and virulent bacteria in tobacco. These observations suggest that (*)NO is integral to the elicitation of cell death associated with these two bacterial pathogens in tobacco.</abstract><cop>Rockville</cop><pub>American Society of Plant Biologists</pub><pmid>16009999</pmid><doi>10.1104/pp.104.055772</doi><tpages>12</tpages></addata></record> |
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| ispartof | Plant physiology (Bethesda), 2005-07, Vol.138 (3), p.1247-1258 |
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| source | JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Breakthrough Technologies Nitric oxide Pathogens Tobacco Wildfires |
| title | Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars1 |
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