M2 muscarinic receptor-mediated inhibition of the Ca2+ current in rat magnocellular cholinergic basal forebrain neurones
1. The actions of muscarinic agonists and antagonists upon the Ca2+ current (ICa) in acutely dissociated magnocellular cholinergic basal forebrain neurones from 11 to 14-day-old postnatal rats were studied using the whole-cell patch-clamp technique. 2. In all cells studied, muscarinic agonists inhib...
Gespeichert in:
| Veröffentlicht in: | The Journal of physiology 1993-07, Vol.466 (1), p.173-189 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 189 |
|---|---|
| container_issue | 1 |
| container_start_page | 173 |
| container_title | The Journal of physiology |
| container_volume | 466 |
| creator | Allen, T G Brown, D A |
| description | 1. The actions of muscarinic agonists and antagonists upon the Ca2+ current (ICa) in acutely dissociated magnocellular cholinergic
basal forebrain neurones from 11 to 14-day-old postnatal rats were studied using the whole-cell patch-clamp technique. 2.
In all cells studied, muscarinic agonists inhibited a transient component of high-voltage-activated (HVA) current, but had
no effect upon the low-voltage-activated (LVA) current. The mean IC50 values for ACh and oxotremorine methiodide (oxo-M),
obtained from non-cumulative dose-response curves, were 204 and 363 nM respectively. Superfusion with the K+ channel blocker,
tetraethylammonium chloride (TEA; 30 mM) shifted the ACh dose-response curve to the right giving an IC50 value of 22:9 microM.
3. Pirenzepine (0.1-1 microM) and methoctramine (0.03-0.3 microM) produced parallel shifts to the right in the agonist dose-response
curves. Schild analysis of the agonist dose ratios yielded pA2 (negative log of the apparent dissociation constant KB) values
for pirenzepine and methoctramine of 6.8 and 8.2 respectively, indicating the involvement of an M2 receptor subtype. 4. In
the presence of GTP-gamma-S (10-500 microM) in the patch pipette, the agonist-induced inhibition of ICa became irreversible.
Dialysis with GDP-beta-S (1 mM) abolished all agonist-induced inhibition of the Ca2+ current. The agonist-induced inhibition
of ICa was totally blocked by pretreatment with pertussis toxin (500 ng ml-1) but unaffected by preincubation with cholera
toxin (500 ng ml-1). Superfusion with 8-bromo cAMP (0.5-1 mM) did not mimic or prevent the effect of agonist application.
5. Inhibition of the Ca2+ current by muscarinic agonists was only partially blocked by omega-conotoxin GVIA (omega-CgTX GVIA),
with approximately 46% of the agonist-sensitive current being resistant to omega-CgTX GVIA. Both the agonist- and omega-CgTX
GVIA-sensitive components of the current were abolished following maximal inhibition of ICa by GTP-gamma-S. 6. These results
indicate that inhibition of the Ca2+ current by muscarinic agonists is mediated via an M2 muscarinic receptor coupled to a
pertussis toxin-sensitive G-protein. The possible modulation of multiple HVA Ca2+ channels by muscarinic agonists and the
role that these receptors may play in presynaptic modulation of neurotransmitter release are discussed. |
| doi_str_mv | 10.1113/jphysiol.1993.sp019715 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1175473</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1709186296</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4953-6fc4f8c93789ebc133d3a4c9e6099888896a25f292281e8fca5daf0b7747f6693</originalsourceid><addsrcrecordid>eNqNkV-P1CAUxYnRrOPqR9DwYKKJ6QilhfJiohP_Zo0-rM-EMpcpmxYqtK7z7aXp7ETf5IWE87vncnIQekbJllLKXt-M3TG50G-plGybRkKloPU9tKEVl4UQkt1HG0LKsmCipg_Ro5RuCKGMSHmBLpqKEi7JBv3-WuJhTkZH553BEQyMU4jFAHunJ9hj5zvXuskFj4PFUwd4p8tX2Mwxgp-yjKOe8KAPPhjo-7nXEZsu9M5DPGTHVifdYxsitFFn2sMcg4f0GD2wuk_w5HRfoh8f3l_vPhVX3z5-3r29Kkwla1ZwayrbGMlEI6E1lLE905WRwHOQJh_JdVnbUpZlQ6GxRtd7bUkrRCUs55Jdojer7zi3OZTJn466V2N0g45HFbRT_yredeoQfilKRV0Jlg1engxi-DlDmtTg0hJVewhzUlQQSRteSp5RvqImhpQi2PMaStTSmrprTS2tqbvW8uDTvz95HjvVlPXnJ13nqnobtTcunbFKyMw1GXu3Yreuh-N_LlfXX74vDxXnOfKS98Vq0rlDd-siqHUsBeNgyss4V1Qt5B8rRci7</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1709186296</pqid></control><display><type>article</type><title>M2 muscarinic receptor-mediated inhibition of the Ca2+ current in rat magnocellular cholinergic basal forebrain neurones</title><source>MEDLINE</source><source>IngentaConnect Free/Open Access Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Allen, T G ; Brown, D A</creator><creatorcontrib>Allen, T G ; Brown, D A</creatorcontrib><description>1. The actions of muscarinic agonists and antagonists upon the Ca2+ current (ICa) in acutely dissociated magnocellular cholinergic
basal forebrain neurones from 11 to 14-day-old postnatal rats were studied using the whole-cell patch-clamp technique. 2.
In all cells studied, muscarinic agonists inhibited a transient component of high-voltage-activated (HVA) current, but had
no effect upon the low-voltage-activated (LVA) current. The mean IC50 values for ACh and oxotremorine methiodide (oxo-M),
obtained from non-cumulative dose-response curves, were 204 and 363 nM respectively. Superfusion with the K+ channel blocker,
tetraethylammonium chloride (TEA; 30 mM) shifted the ACh dose-response curve to the right giving an IC50 value of 22:9 microM.
3. Pirenzepine (0.1-1 microM) and methoctramine (0.03-0.3 microM) produced parallel shifts to the right in the agonist dose-response
curves. Schild analysis of the agonist dose ratios yielded pA2 (negative log of the apparent dissociation constant KB) values
for pirenzepine and methoctramine of 6.8 and 8.2 respectively, indicating the involvement of an M2 receptor subtype. 4. In
the presence of GTP-gamma-S (10-500 microM) in the patch pipette, the agonist-induced inhibition of ICa became irreversible.
Dialysis with GDP-beta-S (1 mM) abolished all agonist-induced inhibition of the Ca2+ current. The agonist-induced inhibition
of ICa was totally blocked by pretreatment with pertussis toxin (500 ng ml-1) but unaffected by preincubation with cholera
toxin (500 ng ml-1). Superfusion with 8-bromo cAMP (0.5-1 mM) did not mimic or prevent the effect of agonist application.
5. Inhibition of the Ca2+ current by muscarinic agonists was only partially blocked by omega-conotoxin GVIA (omega-CgTX GVIA),
with approximately 46% of the agonist-sensitive current being resistant to omega-CgTX GVIA. Both the agonist- and omega-CgTX
GVIA-sensitive components of the current were abolished following maximal inhibition of ICa by GTP-gamma-S. 6. These results
indicate that inhibition of the Ca2+ current by muscarinic agonists is mediated via an M2 muscarinic receptor coupled to a
pertussis toxin-sensitive G-protein. The possible modulation of multiple HVA Ca2+ channels by muscarinic agonists and the
role that these receptors may play in presynaptic modulation of neurotransmitter release are discussed.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1993.sp019715</identifier><identifier>PMID: 8410690</identifier><identifier>CODEN: JPHYA7</identifier><language>eng</language><publisher>Oxford: The Physiological Society</publisher><subject>Acetylcholine - pharmacology ; Animals ; Biological and medical sciences ; Calcium Channel Blockers ; Calcium Channels - drug effects ; Calcium Channels - metabolism ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Cholera Toxin - pharmacology ; Cholinergic Fibers - drug effects ; Cholinergic Fibers - metabolism ; Diamines - pharmacology ; Fundamental and applied biological sciences. Psychology ; GTP-Binding Proteins - metabolism ; Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology ; In Vitro Techniques ; Neurons - drug effects ; Neurons - metabolism ; omega-Conotoxin GVIA ; Oxotremorine - pharmacology ; Parasympatholytics - pharmacology ; Peptides - pharmacology ; Pertussis Toxin ; Pirenzepine - pharmacology ; Prosencephalon - metabolism ; Rats ; Receptors, Muscarinic - classification ; Receptors, Muscarinic - drug effects ; Receptors, Muscarinic - metabolism ; Vertebrates: nervous system and sense organs ; Virulence Factors, Bordetella - pharmacology</subject><ispartof>The Journal of physiology, 1993-07, Vol.466 (1), p.173-189</ispartof><rights>1993 The Physiological Society</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4953-6fc4f8c93789ebc133d3a4c9e6099888896a25f292281e8fca5daf0b7747f6693</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175473/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175473/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,27901,27902,45550,45551,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4791068$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8410690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Allen, T G</creatorcontrib><creatorcontrib>Brown, D A</creatorcontrib><title>M2 muscarinic receptor-mediated inhibition of the Ca2+ current in rat magnocellular cholinergic basal forebrain neurones</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. The actions of muscarinic agonists and antagonists upon the Ca2+ current (ICa) in acutely dissociated magnocellular cholinergic
basal forebrain neurones from 11 to 14-day-old postnatal rats were studied using the whole-cell patch-clamp technique. 2.
In all cells studied, muscarinic agonists inhibited a transient component of high-voltage-activated (HVA) current, but had
no effect upon the low-voltage-activated (LVA) current. The mean IC50 values for ACh and oxotremorine methiodide (oxo-M),
obtained from non-cumulative dose-response curves, were 204 and 363 nM respectively. Superfusion with the K+ channel blocker,
tetraethylammonium chloride (TEA; 30 mM) shifted the ACh dose-response curve to the right giving an IC50 value of 22:9 microM.
3. Pirenzepine (0.1-1 microM) and methoctramine (0.03-0.3 microM) produced parallel shifts to the right in the agonist dose-response
curves. Schild analysis of the agonist dose ratios yielded pA2 (negative log of the apparent dissociation constant KB) values
for pirenzepine and methoctramine of 6.8 and 8.2 respectively, indicating the involvement of an M2 receptor subtype. 4. In
the presence of GTP-gamma-S (10-500 microM) in the patch pipette, the agonist-induced inhibition of ICa became irreversible.
Dialysis with GDP-beta-S (1 mM) abolished all agonist-induced inhibition of the Ca2+ current. The agonist-induced inhibition
of ICa was totally blocked by pretreatment with pertussis toxin (500 ng ml-1) but unaffected by preincubation with cholera
toxin (500 ng ml-1). Superfusion with 8-bromo cAMP (0.5-1 mM) did not mimic or prevent the effect of agonist application.
5. Inhibition of the Ca2+ current by muscarinic agonists was only partially blocked by omega-conotoxin GVIA (omega-CgTX GVIA),
with approximately 46% of the agonist-sensitive current being resistant to omega-CgTX GVIA. Both the agonist- and omega-CgTX
GVIA-sensitive components of the current were abolished following maximal inhibition of ICa by GTP-gamma-S. 6. These results
indicate that inhibition of the Ca2+ current by muscarinic agonists is mediated via an M2 muscarinic receptor coupled to a
pertussis toxin-sensitive G-protein. The possible modulation of multiple HVA Ca2+ channels by muscarinic agonists and the
role that these receptors may play in presynaptic modulation of neurotransmitter release are discussed.</description><subject>Acetylcholine - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium Channel Blockers</subject><subject>Calcium Channels - drug effects</subject><subject>Calcium Channels - metabolism</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Cholera Toxin - pharmacology</subject><subject>Cholinergic Fibers - drug effects</subject><subject>Cholinergic Fibers - metabolism</subject><subject>Diamines - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GTP-Binding Proteins - metabolism</subject><subject>Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>omega-Conotoxin GVIA</subject><subject>Oxotremorine - pharmacology</subject><subject>Parasympatholytics - pharmacology</subject><subject>Peptides - pharmacology</subject><subject>Pertussis Toxin</subject><subject>Pirenzepine - pharmacology</subject><subject>Prosencephalon - metabolism</subject><subject>Rats</subject><subject>Receptors, Muscarinic - classification</subject><subject>Receptors, Muscarinic - drug effects</subject><subject>Receptors, Muscarinic - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Virulence Factors, Bordetella - pharmacology</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV-P1CAUxYnRrOPqR9DwYKKJ6QilhfJiohP_Zo0-rM-EMpcpmxYqtK7z7aXp7ETf5IWE87vncnIQekbJllLKXt-M3TG50G-plGybRkKloPU9tKEVl4UQkt1HG0LKsmCipg_Ro5RuCKGMSHmBLpqKEi7JBv3-WuJhTkZH553BEQyMU4jFAHunJ9hj5zvXuskFj4PFUwd4p8tX2Mwxgp-yjKOe8KAPPhjo-7nXEZsu9M5DPGTHVifdYxsitFFn2sMcg4f0GD2wuk_w5HRfoh8f3l_vPhVX3z5-3r29Kkwla1ZwayrbGMlEI6E1lLE905WRwHOQJh_JdVnbUpZlQ6GxRtd7bUkrRCUs55Jdojer7zi3OZTJn466V2N0g45HFbRT_yredeoQfilKRV0Jlg1engxi-DlDmtTg0hJVewhzUlQQSRteSp5RvqImhpQi2PMaStTSmrprTS2tqbvW8uDTvz95HjvVlPXnJ13nqnobtTcunbFKyMw1GXu3Yreuh-N_LlfXX74vDxXnOfKS98Vq0rlDd-siqHUsBeNgyss4V1Qt5B8rRci7</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>Allen, T G</creator><creator>Brown, D A</creator><general>The Physiological Society</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>19930701</creationdate><title>M2 muscarinic receptor-mediated inhibition of the Ca2+ current in rat magnocellular cholinergic basal forebrain neurones</title><author>Allen, T G ; Brown, D A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4953-6fc4f8c93789ebc133d3a4c9e6099888896a25f292281e8fca5daf0b7747f6693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acetylcholine - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium Channel Blockers</topic><topic>Calcium Channels - drug effects</topic><topic>Calcium Channels - metabolism</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Cholera Toxin - pharmacology</topic><topic>Cholinergic Fibers - drug effects</topic><topic>Cholinergic Fibers - metabolism</topic><topic>Diamines - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GTP-Binding Proteins - metabolism</topic><topic>Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>omega-Conotoxin GVIA</topic><topic>Oxotremorine - pharmacology</topic><topic>Parasympatholytics - pharmacology</topic><topic>Peptides - pharmacology</topic><topic>Pertussis Toxin</topic><topic>Pirenzepine - pharmacology</topic><topic>Prosencephalon - metabolism</topic><topic>Rats</topic><topic>Receptors, Muscarinic - classification</topic><topic>Receptors, Muscarinic - drug effects</topic><topic>Receptors, Muscarinic - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Virulence Factors, Bordetella - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Allen, T G</creatorcontrib><creatorcontrib>Brown, D A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Allen, T G</au><au>Brown, D A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>M2 muscarinic receptor-mediated inhibition of the Ca2+ current in rat magnocellular cholinergic basal forebrain neurones</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>466</volume><issue>1</issue><spage>173</spage><epage>189</epage><pages>173-189</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. The actions of muscarinic agonists and antagonists upon the Ca2+ current (ICa) in acutely dissociated magnocellular cholinergic
basal forebrain neurones from 11 to 14-day-old postnatal rats were studied using the whole-cell patch-clamp technique. 2.
In all cells studied, muscarinic agonists inhibited a transient component of high-voltage-activated (HVA) current, but had
no effect upon the low-voltage-activated (LVA) current. The mean IC50 values for ACh and oxotremorine methiodide (oxo-M),
obtained from non-cumulative dose-response curves, were 204 and 363 nM respectively. Superfusion with the K+ channel blocker,
tetraethylammonium chloride (TEA; 30 mM) shifted the ACh dose-response curve to the right giving an IC50 value of 22:9 microM.
3. Pirenzepine (0.1-1 microM) and methoctramine (0.03-0.3 microM) produced parallel shifts to the right in the agonist dose-response
curves. Schild analysis of the agonist dose ratios yielded pA2 (negative log of the apparent dissociation constant KB) values
for pirenzepine and methoctramine of 6.8 and 8.2 respectively, indicating the involvement of an M2 receptor subtype. 4. In
the presence of GTP-gamma-S (10-500 microM) in the patch pipette, the agonist-induced inhibition of ICa became irreversible.
Dialysis with GDP-beta-S (1 mM) abolished all agonist-induced inhibition of the Ca2+ current. The agonist-induced inhibition
of ICa was totally blocked by pretreatment with pertussis toxin (500 ng ml-1) but unaffected by preincubation with cholera
toxin (500 ng ml-1). Superfusion with 8-bromo cAMP (0.5-1 mM) did not mimic or prevent the effect of agonist application.
5. Inhibition of the Ca2+ current by muscarinic agonists was only partially blocked by omega-conotoxin GVIA (omega-CgTX GVIA),
with approximately 46% of the agonist-sensitive current being resistant to omega-CgTX GVIA. Both the agonist- and omega-CgTX
GVIA-sensitive components of the current were abolished following maximal inhibition of ICa by GTP-gamma-S. 6. These results
indicate that inhibition of the Ca2+ current by muscarinic agonists is mediated via an M2 muscarinic receptor coupled to a
pertussis toxin-sensitive G-protein. The possible modulation of multiple HVA Ca2+ channels by muscarinic agonists and the
role that these receptors may play in presynaptic modulation of neurotransmitter release are discussed.</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>8410690</pmid><doi>10.1113/jphysiol.1993.sp019715</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3751 |
| ispartof | The Journal of physiology, 1993-07, Vol.466 (1), p.173-189 |
| issn | 0022-3751 1469-7793 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1175473 |
| source | MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Acetylcholine - pharmacology Animals Biological and medical sciences Calcium Channel Blockers Calcium Channels - drug effects Calcium Channels - metabolism Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Cholera Toxin - pharmacology Cholinergic Fibers - drug effects Cholinergic Fibers - metabolism Diamines - pharmacology Fundamental and applied biological sciences. Psychology GTP-Binding Proteins - metabolism Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology In Vitro Techniques Neurons - drug effects Neurons - metabolism omega-Conotoxin GVIA Oxotremorine - pharmacology Parasympatholytics - pharmacology Peptides - pharmacology Pertussis Toxin Pirenzepine - pharmacology Prosencephalon - metabolism Rats Receptors, Muscarinic - classification Receptors, Muscarinic - drug effects Receptors, Muscarinic - metabolism Vertebrates: nervous system and sense organs Virulence Factors, Bordetella - pharmacology |
| title | M2 muscarinic receptor-mediated inhibition of the Ca2+ current in rat magnocellular cholinergic basal forebrain neurones |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T16%3A20%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=M2%20muscarinic%20receptor-mediated%20inhibition%20of%20the%20Ca2+%20current%20in%20rat%20magnocellular%20cholinergic%20basal%20forebrain%20neurones&rft.jtitle=The%20Journal%20of%20physiology&rft.au=Allen,%20T%20G&rft.date=1993-07-01&rft.volume=466&rft.issue=1&rft.spage=173&rft.epage=189&rft.pages=173-189&rft.issn=0022-3751&rft.eissn=1469-7793&rft.coden=JPHYA7&rft_id=info:doi/10.1113/jphysiol.1993.sp019715&rft_dat=%3Cproquest_pubme%3E1709186296%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1709186296&rft_id=info:pmid/8410690&rfr_iscdi=true |