Volume-dependent K+ and Cl- fluxes in rat thymocytes
1. Hypotonic stress unmasked inward and outward K+ and Cl- movements in rat thymocytes. This KCl flux stimulation was reduced by DIOA (dihydroindenyl-oxy-alkanoic acid), but not by DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonate), quinidine, DPAC 144 (5-nitro-2-(2-phenylethyl-amino)-be...
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| Veröffentlicht in: | The Journal of physiology 1993-06, Vol.465 (1), p.387-401 |
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| creator | A Soler R Rota P Hannaert E J Cragoe, Jr R P Garay |
| description | 1. Hypotonic stress unmasked inward and outward K+ and Cl- movements in rat thymocytes. This KCl flux stimulation was reduced
by DIOA (dihydroindenyl-oxy-alkanoic acid), but not by DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonate), quinidine, DPAC
144 (5-nitro-2-(2-phenylethyl-amino)-benzoic acid), bumetanide or ouabain. 2. In isotonic media (308 +/- 5 mosmol kg-1), the
cells exhibited the following DIOA-sensitive fluxes: (i) a K+ efflux of 42.7 +/- 17.1 mmol (l cells.h)-1 (mean +/- S.D., n
= 7), (ii) a Cl- efflux of 68 +/- 21 mmol (l cells.h)-1 (n = 3), (iii) a Rb+ influx of 9.7 +/- 3.9 mmol (l cells.h)-1 (n =
6) and (iv) a Cl- influx of 9.4 +/- 4.1 mmol (l cells.h)-1 (n = 6). 3. Hypotonic shock (183-200 mosmol kg-1) induced a sevenfold
stimulation of DIOA-sensitive K+ and Cl- effluxes and a twofold stimulation of DIOA-sensitive Rb+ and Cl- influxes (with a
Rb+ to Cl- stoichiometry of 1.04 +/- 0.31; mean +/- S.D., n = 6). 4. The DIOA-sensitive membrane carrier catalysed net outward
KCl extrusion (the outward/inward flux ratio was 5-7 in isotonic media and 20 in hypotonic media at 189 mosmol kg-1). Inhibition
of DIOA-sensitive 36Cl- efflux by cell K+ depletion suggested coupling of outward K+ and Cl- fluxes. Conversely, inward K+
and Cl- fluxes were found to be uncoupled in NO3- media and in K(+)-free media. 5. The results clearly show that rat thymocyte
membranes possess a 1:1 K(+)-Cl- co-transport system which is strongly activated by hypotonic shock and catalyses net KCl
extrusion. |
| doi_str_mv | 10.1113/jphysiol.1993.sp019682 |
| format | Article |
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by DIOA (dihydroindenyl-oxy-alkanoic acid), but not by DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonate), quinidine, DPAC
144 (5-nitro-2-(2-phenylethyl-amino)-benzoic acid), bumetanide or ouabain. 2. In isotonic media (308 +/- 5 mosmol kg-1), the
cells exhibited the following DIOA-sensitive fluxes: (i) a K+ efflux of 42.7 +/- 17.1 mmol (l cells.h)-1 (mean +/- S.D., n
= 7), (ii) a Cl- efflux of 68 +/- 21 mmol (l cells.h)-1 (n = 3), (iii) a Rb+ influx of 9.7 +/- 3.9 mmol (l cells.h)-1 (n =
6) and (iv) a Cl- influx of 9.4 +/- 4.1 mmol (l cells.h)-1 (n = 6). 3. Hypotonic shock (183-200 mosmol kg-1) induced a sevenfold
stimulation of DIOA-sensitive K+ and Cl- effluxes and a twofold stimulation of DIOA-sensitive Rb+ and Cl- influxes (with a
Rb+ to Cl- stoichiometry of 1.04 +/- 0.31; mean +/- S.D., n = 6). 4. The DIOA-sensitive membrane carrier catalysed net outward
KCl extrusion (the outward/inward flux ratio was 5-7 in isotonic media and 20 in hypotonic media at 189 mosmol kg-1). Inhibition
of DIOA-sensitive 36Cl- efflux by cell K+ depletion suggested coupling of outward K+ and Cl- fluxes. Conversely, inward K+
and Cl- fluxes were found to be uncoupled in NO3- media and in K(+)-free media. 5. The results clearly show that rat thymocyte
membranes possess a 1:1 K(+)-Cl- co-transport system which is strongly activated by hypotonic shock and catalyses net KCl
extrusion.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1993.sp019682</identifier><identifier>PMID: 8229841</identifier><identifier>CODEN: JPHYA7</identifier><language>eng</language><publisher>Oxford: The Physiological Society</publisher><subject>Animals ; Biological and medical sciences ; Calcium - metabolism ; Carboxylic Acids - pharmacology ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cell physiology ; Chlorides - metabolism ; Chlorine ; Cytosol - metabolism ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Hypotonic Solutions ; In Vitro Techniques ; Indenes - pharmacology ; Male ; Membrane and intracellular transports ; Membrane Potentials - drug effects ; Membrane Potentials - physiology ; Molecular and cellular biology ; Potassium - metabolism ; Potassium Channels - drug effects ; Potassium Channels - metabolism ; Radioisotopes ; Rats ; Rats, Wistar ; Rubidium - metabolism ; Thymus Gland - cytology ; Thymus Gland - drug effects ; Thymus Gland - metabolism</subject><ispartof>The Journal of physiology, 1993-06, Vol.465 (1), p.387-401</ispartof><rights>1993 The Physiological Society</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5297-daab023bf32a30b6d02a50f618504e78833c3d425275aac2c9342cc45935fb0e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175435/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175435/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,27901,27902,45550,45551,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4774901$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8229841$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>A Soler</creatorcontrib><creatorcontrib>R Rota</creatorcontrib><creatorcontrib>P Hannaert</creatorcontrib><creatorcontrib>E J Cragoe, Jr</creatorcontrib><creatorcontrib>R P Garay</creatorcontrib><title>Volume-dependent K+ and Cl- fluxes in rat thymocytes</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. Hypotonic stress unmasked inward and outward K+ and Cl- movements in rat thymocytes. This KCl flux stimulation was reduced
by DIOA (dihydroindenyl-oxy-alkanoic acid), but not by DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonate), quinidine, DPAC
144 (5-nitro-2-(2-phenylethyl-amino)-benzoic acid), bumetanide or ouabain. 2. In isotonic media (308 +/- 5 mosmol kg-1), the
cells exhibited the following DIOA-sensitive fluxes: (i) a K+ efflux of 42.7 +/- 17.1 mmol (l cells.h)-1 (mean +/- S.D., n
= 7), (ii) a Cl- efflux of 68 +/- 21 mmol (l cells.h)-1 (n = 3), (iii) a Rb+ influx of 9.7 +/- 3.9 mmol (l cells.h)-1 (n =
6) and (iv) a Cl- influx of 9.4 +/- 4.1 mmol (l cells.h)-1 (n = 6). 3. Hypotonic shock (183-200 mosmol kg-1) induced a sevenfold
stimulation of DIOA-sensitive K+ and Cl- effluxes and a twofold stimulation of DIOA-sensitive Rb+ and Cl- influxes (with a
Rb+ to Cl- stoichiometry of 1.04 +/- 0.31; mean +/- S.D., n = 6). 4. The DIOA-sensitive membrane carrier catalysed net outward
KCl extrusion (the outward/inward flux ratio was 5-7 in isotonic media and 20 in hypotonic media at 189 mosmol kg-1). Inhibition
of DIOA-sensitive 36Cl- efflux by cell K+ depletion suggested coupling of outward K+ and Cl- fluxes. Conversely, inward K+
and Cl- fluxes were found to be uncoupled in NO3- media and in K(+)-free media. 5. The results clearly show that rat thymocyte
membranes possess a 1:1 K(+)-Cl- co-transport system which is strongly activated by hypotonic shock and catalyses net KCl
extrusion.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Carboxylic Acids - pharmacology</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cell physiology</subject><subject>Chlorides - metabolism</subject><subject>Chlorine</subject><subject>Cytosol - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hypotonic Solutions</subject><subject>In Vitro Techniques</subject><subject>Indenes - pharmacology</subject><subject>Male</subject><subject>Membrane and intracellular transports</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - physiology</subject><subject>Molecular and cellular biology</subject><subject>Potassium - metabolism</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium Channels - metabolism</subject><subject>Radioisotopes</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rubidium - metabolism</subject><subject>Thymus Gland - cytology</subject><subject>Thymus Gland - drug effects</subject><subject>Thymus Gland - metabolism</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtLwzAcx4Moc07_BKUHwYN05tk0F0GH74EepteQpuka6Yumc_a_t6Xb0JunQL6vHx8AzhCcIoTI1WeVts6W2RQJQaaugkgEId4DY0QD4XMuyD4YQ4ixTzhDh-DIuU8IEYFCjMAoxFiEFI0B_SizVW782FSmiE3ReC-Xnipib5b5XpKtvo3zbOHVqvGatM1L3TbGHYODRGXOnGzeCXi_v1vMHv3568PT7Gbua4YF92OlIohJlBCsCIyCGGLFYBKgkEFqeBgSoklMMcOcKaWxFoRirSkThCURNGQCrofeahXlJtbdebXKZFXbXNWtLJWVf5XCpnJZfkmEOKOEdQXBUKDr0rnaJLssgrLHKLcYZY9RbjF2wdPfy7vYhlunn2905bTKkloV2rqdjXJOBextt4NtbTPT_nNcLp7f-g8aMERC3pVcDCWpXaZrWxs5xFyprWm6sYBJJHvnD1rqoLg</recordid><startdate>19930601</startdate><enddate>19930601</enddate><creator>A Soler</creator><creator>R Rota</creator><creator>P Hannaert</creator><creator>E J Cragoe, Jr</creator><creator>R P Garay</creator><general>The Physiological Society</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19930601</creationdate><title>Volume-dependent K+ and Cl- fluxes in rat thymocytes</title><author>A Soler ; R Rota ; P Hannaert ; E J Cragoe, Jr ; R P Garay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5297-daab023bf32a30b6d02a50f618504e78833c3d425275aac2c9342cc45935fb0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Carboxylic Acids - pharmacology</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Cell physiology</topic><topic>Chlorides - metabolism</topic><topic>Chlorine</topic><topic>Cytosol - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hypotonic Solutions</topic><topic>In Vitro Techniques</topic><topic>Indenes - pharmacology</topic><topic>Male</topic><topic>Membrane and intracellular transports</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - physiology</topic><topic>Molecular and cellular biology</topic><topic>Potassium - metabolism</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - metabolism</topic><topic>Radioisotopes</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Rubidium - metabolism</topic><topic>Thymus Gland - cytology</topic><topic>Thymus Gland - drug effects</topic><topic>Thymus Gland - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>A Soler</creatorcontrib><creatorcontrib>R Rota</creatorcontrib><creatorcontrib>P Hannaert</creatorcontrib><creatorcontrib>E J Cragoe, Jr</creatorcontrib><creatorcontrib>R P Garay</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>A Soler</au><au>R Rota</au><au>P Hannaert</au><au>E J Cragoe, Jr</au><au>R P Garay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Volume-dependent K+ and Cl- fluxes in rat thymocytes</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1993-06-01</date><risdate>1993</risdate><volume>465</volume><issue>1</issue><spage>387</spage><epage>401</epage><pages>387-401</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. Hypotonic stress unmasked inward and outward K+ and Cl- movements in rat thymocytes. This KCl flux stimulation was reduced
by DIOA (dihydroindenyl-oxy-alkanoic acid), but not by DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonate), quinidine, DPAC
144 (5-nitro-2-(2-phenylethyl-amino)-benzoic acid), bumetanide or ouabain. 2. In isotonic media (308 +/- 5 mosmol kg-1), the
cells exhibited the following DIOA-sensitive fluxes: (i) a K+ efflux of 42.7 +/- 17.1 mmol (l cells.h)-1 (mean +/- S.D., n
= 7), (ii) a Cl- efflux of 68 +/- 21 mmol (l cells.h)-1 (n = 3), (iii) a Rb+ influx of 9.7 +/- 3.9 mmol (l cells.h)-1 (n =
6) and (iv) a Cl- influx of 9.4 +/- 4.1 mmol (l cells.h)-1 (n = 6). 3. Hypotonic shock (183-200 mosmol kg-1) induced a sevenfold
stimulation of DIOA-sensitive K+ and Cl- effluxes and a twofold stimulation of DIOA-sensitive Rb+ and Cl- influxes (with a
Rb+ to Cl- stoichiometry of 1.04 +/- 0.31; mean +/- S.D., n = 6). 4. The DIOA-sensitive membrane carrier catalysed net outward
KCl extrusion (the outward/inward flux ratio was 5-7 in isotonic media and 20 in hypotonic media at 189 mosmol kg-1). Inhibition
of DIOA-sensitive 36Cl- efflux by cell K+ depletion suggested coupling of outward K+ and Cl- fluxes. Conversely, inward K+
and Cl- fluxes were found to be uncoupled in NO3- media and in K(+)-free media. 5. The results clearly show that rat thymocyte
membranes possess a 1:1 K(+)-Cl- co-transport system which is strongly activated by hypotonic shock and catalyses net KCl
extrusion.</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>8229841</pmid><doi>10.1113/jphysiol.1993.sp019682</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Calcium - metabolism Carboxylic Acids - pharmacology Cell Membrane - drug effects Cell Membrane - metabolism Cell physiology Chlorides - metabolism Chlorine Cytosol - metabolism Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hypotonic Solutions In Vitro Techniques Indenes - pharmacology Male Membrane and intracellular transports Membrane Potentials - drug effects Membrane Potentials - physiology Molecular and cellular biology Potassium - metabolism Potassium Channels - drug effects Potassium Channels - metabolism Radioisotopes Rats Rats, Wistar Rubidium - metabolism Thymus Gland - cytology Thymus Gland - drug effects Thymus Gland - metabolism |
| title | Volume-dependent K+ and Cl- fluxes in rat thymocytes |
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