Molecular Immunolabeling with Recombinant Single-Chain Variable Fragment (scFv) Antibodies Designed with Metal-Binding Domains

To study the molecular structure and function of gene products in situ, we developed a molecular immunolabeling technology. Starting with cDNA from hybridomas producing monoclonal antibodies against biotin, catalase, and superoxide dismutase, we bioengineered recombinant single-chain variable fragme...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2002-01, Vol.99 (1), p.213-218
Hauptverfasser:	Malecki, Marek, Hsu, Annie, Truong, Lynn, Sanchez, Sylvia
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antibodies - chemistry Antigen-Antibody Reactions - immunology Antigens Bioengineering Biological Sciences Biotinylation Cell Line Chloramphenicol O-Acetyltransferase - metabolism DNA, Complementary - metabolism Enzyme linked immunosorbent assay Hybridomas - immunology Imaging Immunoglobulin Fragments - chemistry Immunoglobulin Fragments - immunology Immunoglobulin G - chemistry Immunoglobulin G - metabolism Immunohistochemistry Kinetics Magnetic resonance imaging Mice Mice, Inbred BALB C Molecular imaging Molecules Neurons - metabolism Peroxisomes Peroxisomes - metabolism Plasmids - metabolism Product labeling Protein Binding Protein Structure, Tertiary Radioimmunoassay Superoxide Dismutase - metabolism Surface Plasmon Resonance
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