A novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system

There is an increasing demand for easy, high-throughput (HTP) methods for protein engineering to support advances in the development of structural biology, bioinformatics and drug design. Here, we describe an N- and C-terminal cloning method utilizing Gateway cloning technology that we have adopted...

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Veröffentlicht in:	Nucleic acids research 2005-01, Vol.33 (12), p.e109-e109
Hauptverfasser:	Suzuki, Yasuhiro, Kagawa, Naoko, Fujino, Toru, Sumiya, Tsuyoshi, Andoh, Taichi, Ishikawa, Kumiko, Kimura, Rie, Kemmochi, Kiyokazu, Ohta, Tsutomu, Tanaka, Shigeo
Format:	Artikel
Sprache:	eng
Schlagworte:	Cloning, Molecular - methods DNA Shuffling DNA, Circular - chemistry Escherichia coli - genetics Genetic Vectors Methods Online Mutagenesis, Site-Directed Plasmids - chemistry Polymerase Chain Reaction Recombinant Fusion Proteins - genetics Recombination, Genetic Sequence Homology, Nucleic Acid Transformation, Bacterial
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