Full‐spectrum cannabidiol reduces UVB damage through the inhibition of TGF‐β1 and the NLRP3 inflammasome
The thermodynamic characteristics, antioxidant potential, and photoprotective benefits of full‐spectrum cannabidiol (FS‐CBD) against UVB‐induced cellular death were examined in this study. In silico analysis of CBD showed antioxidant capacity via proton donation and UV absorption at 209.09, 254.73,...
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| creator | Urrutia‐Ortega, I. M. Valencia, I. Ispanixtlahuatl‐Meraz, O. Benítez‐Flores, J. C. Espinosa‐González, A. M. Estrella‐Parra, E. A. Flores‐Ortiz, C. M. Chirino, Y. I. Avila‐Acevedo, J. G. |
| description | The thermodynamic characteristics, antioxidant potential, and photoprotective benefits of full‐spectrum cannabidiol (FS‐CBD) against UVB‐induced cellular death were examined in this study. In silico analysis of CBD showed antioxidant capacity via proton donation and UV absorption at 209.09, 254.73, and 276.95 nm, according to the HAT and SPLET methodologies. FS‐CBD protected against UVB‐induced bacterial death for 30 min. FS‐CBD protected against UVB‐induced cell death by 42% (1.5 μg/mL) and 35% (3.5 μg/mL) in an in vitro keratinocyte cell model. An in vivo acute irradiated CD‐1et/et mouse model (UVB‐irradiated for 5 min) presented very low photoprotection when FS‐CBD was applied cutaneously, as determined by histological analyses. In vivo skin samples showed that FS‐CBD regulated inflammatory responses by inhibiting the inflammatory markers TGF‐β1 and NLRP3. The docking analysis showed that the CBD molecule had a high affinity for TGF‐β1 and NLRP3, indicating that protection against inflammation might be mediated by blocking these proinflammatory molecules. This result was corroborated by the docking interactions between CBD and TGF‐β1 and NLRP3, which resulted in a high affinity and inhibition of both proteins The present work suggested a FS‐CBD moderate photoprotective agent against UVB light‐induced skin damage and that this effect is partially mediated by its anti‐inflammatory activity.
Photoprotective and antiinflammatory benefits of full‐spectrum cannabidiol (FS‐CBD) against UVB‐induced cellular death were examined in this study. FS‐CBD protected against UVB‐induced bacterial death for 30 min. FS‐CBD protected against UVB‐induced cells, in an in vitro keratinocyte cell model. An in vivo acute irradiated CD‐1et/et mouse model presented very low photoprotection when FS‐CBD was applied cutaneously, as determined by histological analysis. In vivo skin samples showed that FS‐CBD regulated inflammatory responses by repressing the inflammatory markers TGF‐β1 and NLRP3. In silico analyses of FS‐CBD showed inhibitory capacity on TGF‐β1, TGF‐βR1, and NLRP3 reducing inflammatory UVB‐induced damage. |
| doi_str_mv | 10.1111/php.13993 |
| format | Article |
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Photoprotective and antiinflammatory benefits of full‐spectrum cannabidiol (FS‐CBD) against UVB‐induced cellular death were examined in this study. FS‐CBD protected against UVB‐induced bacterial death for 30 min. FS‐CBD protected against UVB‐induced cells, in an in vitro keratinocyte cell model. An in vivo acute irradiated CD‐1et/et mouse model presented very low photoprotection when FS‐CBD was applied cutaneously, as determined by histological analysis. In vivo skin samples showed that FS‐CBD regulated inflammatory responses by repressing the inflammatory markers TGF‐β1 and NLRP3. In silico analyses of FS‐CBD showed inhibitory capacity on TGF‐β1, TGF‐βR1, and NLRP3 reducing inflammatory UVB‐induced damage.</description><identifier>ISSN: 0031-8655</identifier><identifier>ISSN: 1751-1097</identifier><identifier>EISSN: 1751-1097</identifier><identifier>DOI: 10.1111/php.13993</identifier><identifier>PMID: 38958000</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Affinity ; Animals ; Anti-inflammatory agents ; Antioxidants ; Antioxidants - chemistry ; Antioxidants - pharmacology ; cannabichromene ; Cannabidiol ; Cannabidiol - chemistry ; Cannabidiol - pharmacology ; Cannabinoids ; CD‐1et/et mice ; Cell death ; Damage ; E. coli ; full spectrum ; Humans ; In vivo methods and tests ; inflammasome ; Inflammasomes ; Inflammasomes - metabolism ; Inflammation ; keratinocytes ; Keratinocytes - drug effects ; Keratinocytes - radiation effects ; Mice ; Molecular Docking Simulation ; Mortality ; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism ; NLRP3 ; photoprotective ; Radiation-Protective Agents - chemistry ; Radiation-Protective Agents - pharmacology ; Skin - drug effects ; Skin - metabolism ; Skin - pathology ; Skin - radiation effects ; TGF‐β1 ; Transforming Growth Factor beta1 - metabolism ; Ultraviolet Rays ; UVB</subject><ispartof>Photochemistry and photobiology, 2025-01, Vol.101 (1), p.83-105</ispartof><rights>2024 The Author(s). published by Wiley Periodicals LLC on behalf of American Society for Photobiology.</rights><rights>2024 The Author(s). Photochemistry and Photobiology published by Wiley Periodicals LLC on behalf of American Society for Photobiology.</rights><rights>2024. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4043-3305932e243e1325d2b87be509ffc85a7786aac23042ae2c4e012d8ac768ab0a3</cites><orcidid>0000-0002-0552-5792 ; 0009-0002-7963-2707 ; 0000-0002-2831-1981</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fphp.13993$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fphp.13993$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38958000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Urrutia‐Ortega, I. M.</creatorcontrib><creatorcontrib>Valencia, I.</creatorcontrib><creatorcontrib>Ispanixtlahuatl‐Meraz, O.</creatorcontrib><creatorcontrib>Benítez‐Flores, J. C.</creatorcontrib><creatorcontrib>Espinosa‐González, A. M.</creatorcontrib><creatorcontrib>Estrella‐Parra, E. A.</creatorcontrib><creatorcontrib>Flores‐Ortiz, C. M.</creatorcontrib><creatorcontrib>Chirino, Y. I.</creatorcontrib><creatorcontrib>Avila‐Acevedo, J. G.</creatorcontrib><title>Full‐spectrum cannabidiol reduces UVB damage through the inhibition of TGF‐β1 and the NLRP3 inflammasome</title><title>Photochemistry and photobiology</title><addtitle>Photochem Photobiol</addtitle><description>The thermodynamic characteristics, antioxidant potential, and photoprotective benefits of full‐spectrum cannabidiol (FS‐CBD) against UVB‐induced cellular death were examined in this study. In silico analysis of CBD showed antioxidant capacity via proton donation and UV absorption at 209.09, 254.73, and 276.95 nm, according to the HAT and SPLET methodologies. FS‐CBD protected against UVB‐induced bacterial death for 30 min. FS‐CBD protected against UVB‐induced cell death by 42% (1.5 μg/mL) and 35% (3.5 μg/mL) in an in vitro keratinocyte cell model. An in vivo acute irradiated CD‐1et/et mouse model (UVB‐irradiated for 5 min) presented very low photoprotection when FS‐CBD was applied cutaneously, as determined by histological analyses. In vivo skin samples showed that FS‐CBD regulated inflammatory responses by inhibiting the inflammatory markers TGF‐β1 and NLRP3. The docking analysis showed that the CBD molecule had a high affinity for TGF‐β1 and NLRP3, indicating that protection against inflammation might be mediated by blocking these proinflammatory molecules. This result was corroborated by the docking interactions between CBD and TGF‐β1 and NLRP3, which resulted in a high affinity and inhibition of both proteins The present work suggested a FS‐CBD moderate photoprotective agent against UVB light‐induced skin damage and that this effect is partially mediated by its anti‐inflammatory activity.
Photoprotective and antiinflammatory benefits of full‐spectrum cannabidiol (FS‐CBD) against UVB‐induced cellular death were examined in this study. FS‐CBD protected against UVB‐induced bacterial death for 30 min. FS‐CBD protected against UVB‐induced cells, in an in vitro keratinocyte cell model. An in vivo acute irradiated CD‐1et/et mouse model presented very low photoprotection when FS‐CBD was applied cutaneously, as determined by histological analysis. In vivo skin samples showed that FS‐CBD regulated inflammatory responses by repressing the inflammatory markers TGF‐β1 and NLRP3. In silico analyses of FS‐CBD showed inhibitory capacity on TGF‐β1, TGF‐βR1, and NLRP3 reducing inflammatory UVB‐induced damage.</description><subject>Affinity</subject><subject>Animals</subject><subject>Anti-inflammatory agents</subject><subject>Antioxidants</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>cannabichromene</subject><subject>Cannabidiol</subject><subject>Cannabidiol - chemistry</subject><subject>Cannabidiol - pharmacology</subject><subject>Cannabinoids</subject><subject>CD‐1et/et mice</subject><subject>Cell death</subject><subject>Damage</subject><subject>E. coli</subject><subject>full spectrum</subject><subject>Humans</subject><subject>In vivo methods and tests</subject><subject>inflammasome</subject><subject>Inflammasomes</subject><subject>Inflammasomes - metabolism</subject><subject>Inflammation</subject><subject>keratinocytes</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - radiation effects</subject><subject>Mice</subject><subject>Molecular Docking Simulation</subject><subject>Mortality</subject><subject>NLR Family, Pyrin Domain-Containing 3 Protein - metabolism</subject><subject>NLRP3</subject><subject>photoprotective</subject><subject>Radiation-Protective Agents - chemistry</subject><subject>Radiation-Protective Agents - pharmacology</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><subject>Skin - pathology</subject><subject>Skin - radiation effects</subject><subject>TGF‐β1</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>Ultraviolet Rays</subject><subject>UVB</subject><issn>0031-8655</issn><issn>1751-1097</issn><issn>1751-1097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAQhi0EosvCgRdAkbjQQ9pxHK-TE4Kq2yKtYIVartbEmWxcJXGwE1BvPALPwoPwEDwJplsqQGIuc5hPn_7Rz9hTDkc8zvHYjkdclKW4xxZcSZ5yKNV9tgAQPC1WUh6wRyFcAfC8VPwhOxBFKQsAWLB-PXfdjy9fw0hm8nOfGBwGrGxtXZd4qmdDIbn88DqpsccdJVPr3bxr46bEDq2t7GTdkLgmuThbR8_3bzzBob65v92834pINR32PQbX02P2oMEu0JPbvWSX69OLk_N08-7szcmrTWpyyEUqBMhSZJTlgrjIZJ1VhapIQtk0ppCoVLFCNJmAPEPKTE7As7pAo1YFVoBiyV7uveNc9VQbGiaPnR697dFfa4dW_30ZbKt37pPmXAkFvIyGF7cG7z7OFCbd22Co63AgNwctQEmhijxGXLLn_6BXbvZD_E8LLlfApcggUod7yngXgqfmLg0H_atFHVvUNy1G9tmf8e_I37VF4HgPfLYdXf_fpLfn273yJw-DqPQ</recordid><startdate>202501</startdate><enddate>202501</enddate><creator>Urrutia‐Ortega, I. 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M.</au><au>Valencia, I.</au><au>Ispanixtlahuatl‐Meraz, O.</au><au>Benítez‐Flores, J. C.</au><au>Espinosa‐González, A. M.</au><au>Estrella‐Parra, E. A.</au><au>Flores‐Ortiz, C. M.</au><au>Chirino, Y. I.</au><au>Avila‐Acevedo, J. G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Full‐spectrum cannabidiol reduces UVB damage through the inhibition of TGF‐β1 and the NLRP3 inflammasome</atitle><jtitle>Photochemistry and photobiology</jtitle><addtitle>Photochem Photobiol</addtitle><date>2025-01</date><risdate>2025</risdate><volume>101</volume><issue>1</issue><spage>83</spage><epage>105</epage><pages>83-105</pages><issn>0031-8655</issn><issn>1751-1097</issn><eissn>1751-1097</eissn><abstract>The thermodynamic characteristics, antioxidant potential, and photoprotective benefits of full‐spectrum cannabidiol (FS‐CBD) against UVB‐induced cellular death were examined in this study. In silico analysis of CBD showed antioxidant capacity via proton donation and UV absorption at 209.09, 254.73, and 276.95 nm, according to the HAT and SPLET methodologies. FS‐CBD protected against UVB‐induced bacterial death for 30 min. FS‐CBD protected against UVB‐induced cell death by 42% (1.5 μg/mL) and 35% (3.5 μg/mL) in an in vitro keratinocyte cell model. An in vivo acute irradiated CD‐1et/et mouse model (UVB‐irradiated for 5 min) presented very low photoprotection when FS‐CBD was applied cutaneously, as determined by histological analyses. In vivo skin samples showed that FS‐CBD regulated inflammatory responses by inhibiting the inflammatory markers TGF‐β1 and NLRP3. The docking analysis showed that the CBD molecule had a high affinity for TGF‐β1 and NLRP3, indicating that protection against inflammation might be mediated by blocking these proinflammatory molecules. This result was corroborated by the docking interactions between CBD and TGF‐β1 and NLRP3, which resulted in a high affinity and inhibition of both proteins The present work suggested a FS‐CBD moderate photoprotective agent against UVB light‐induced skin damage and that this effect is partially mediated by its anti‐inflammatory activity.
Photoprotective and antiinflammatory benefits of full‐spectrum cannabidiol (FS‐CBD) against UVB‐induced cellular death were examined in this study. FS‐CBD protected against UVB‐induced bacterial death for 30 min. FS‐CBD protected against UVB‐induced cells, in an in vitro keratinocyte cell model. An in vivo acute irradiated CD‐1et/et mouse model presented very low photoprotection when FS‐CBD was applied cutaneously, as determined by histological analysis. In vivo skin samples showed that FS‐CBD regulated inflammatory responses by repressing the inflammatory markers TGF‐β1 and NLRP3. In silico analyses of FS‐CBD showed inhibitory capacity on TGF‐β1, TGF‐βR1, and NLRP3 reducing inflammatory UVB‐induced damage.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>38958000</pmid><doi>10.1111/php.13993</doi><tpages>23</tpages><orcidid>https://orcid.org/0000-0002-0552-5792</orcidid><orcidid>https://orcid.org/0009-0002-7963-2707</orcidid><orcidid>https://orcid.org/0000-0002-2831-1981</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Affinity Animals Anti-inflammatory agents Antioxidants Antioxidants - chemistry Antioxidants - pharmacology cannabichromene Cannabidiol Cannabidiol - chemistry Cannabidiol - pharmacology Cannabinoids CD‐1et/et mice Cell death Damage E. coli full spectrum Humans In vivo methods and tests inflammasome Inflammasomes Inflammasomes - metabolism Inflammation keratinocytes Keratinocytes - drug effects Keratinocytes - radiation effects Mice Molecular Docking Simulation Mortality NLR Family, Pyrin Domain-Containing 3 Protein - metabolism NLRP3 photoprotective Radiation-Protective Agents - chemistry Radiation-Protective Agents - pharmacology Skin - drug effects Skin - metabolism Skin - pathology Skin - radiation effects TGF‐β1 Transforming Growth Factor beta1 - metabolism Ultraviolet Rays UVB |
| title | Full‐spectrum cannabidiol reduces UVB damage through the inhibition of TGF‐β1 and the NLRP3 inflammasome |
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