GTP hydrolysis by arf-1 mediates sorting and concentration of Golgi resident enzymes into functional COP I vesicles

Upon addition of GTPγS to in vitro budding reactions, COP I vesicles form but retain their coat, making them easy to isolate and analyze. We have developed an in vitro budding assay that reconstitutes the formation of COP I‐derived vesicles under conditions where GTP hydrolysis can occur. Once forme...

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Veröffentlicht in:	The EMBO journal 1999-09, Vol.18 (18), p.4935-4948
Hauptverfasser:	Lanoix, Joel, Ouwendijk, Joke, Lin, Chung-Chih, Stark, Annika, Love, Harold D., Ostermann, Joachim, Nilsson, Tommy
Format:	Artikel
Sprache:	eng
Schlagworte:	ADP-Ribosylation Factor 1 - metabolism Animals arf-1 Cattle Coat Protein Complex I - metabolism Coatomer Protein - metabolism COP I vesicles Golgi Apparatus - drug effects Golgi Apparatus - metabolism Golgi Apparatus - ultrastructure Golgi resident enzymes GTP hydrolysis Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology Guanosine Triphosphate - metabolism HeLa Cells Humans Hydrolysis In Vitro Techniques Liver - drug effects Liver - metabolism Liver - ultrastructure Mannosidases - metabolism Membranes Microscopy, Electron Models, Biological Organelles - drug effects Organelles - metabolism Organelles - ultrastructure Rats sorting
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