Cytokinesis mediated through the recruitment of cortexillins into the cleavage furrow
The fact that substrate‐anchored Dictyostelium cells undergo cytokinesis in the absence of myosin II underscores the importance of other proteins in enabling the cleavage furrow to constrict. Cortexillins, a pair of actin‐bundling proteins, are required for normal cleavage. They are targeted to the...
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| Veröffentlicht in: | The EMBO journal 1999-02, Vol.18 (3), p.586-594 |
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| creator | Weber, I Gerisch, G Heizer, C Murphy, J Badelt, K Stock, A Schwartz, J.M Faix, J |
| description | The fact that substrate‐anchored
Dictyostelium
cells undergo cytokinesis in the absence of myosin II underscores the importance of other proteins in enabling the cleavage furrow to constrict. Cortexillins, a pair of actin‐bundling proteins, are required for normal cleavage. They are targeted to the incipient furrow in wild‐type and, more prominently, in myosin II‐null cells. No other F‐actin bundling or cross‐linking protein tested is co‐localized. Green fluorescent protein fusions show that the N‐terminal actin‐binding domain of cortexillin I is dispensable and the C‐terminal region is sufficient for translocation to the furrow and the rescue of cytokinesis. Cortexillins are suggested to have a targeting signal for coupling to a myosin II‐independent system that directs transport of membrane proteins to the cleavage furrow. |
| doi_str_mv | 10.1093/emboj/18.3.586 |
| format | Article |
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Dictyostelium
cells undergo cytokinesis in the absence of myosin II underscores the importance of other proteins in enabling the cleavage furrow to constrict. Cortexillins, a pair of actin‐bundling proteins, are required for normal cleavage. They are targeted to the incipient furrow in wild‐type and, more prominently, in myosin II‐null cells. No other F‐actin bundling or cross‐linking protein tested is co‐localized. Green fluorescent protein fusions show that the N‐terminal actin‐binding domain of cortexillin I is dispensable and the C‐terminal region is sufficient for translocation to the furrow and the rescue of cytokinesis. Cortexillins are suggested to have a targeting signal for coupling to a myosin II‐independent system that directs transport of membrane proteins to the cleavage furrow.</description><identifier>ISSN: 0261-4189</identifier><identifier>ISSN: 1460-2075</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1093/emboj/18.3.586</identifier><identifier>PMID: 9927418</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>actin ; Actins - metabolism ; animal proteins ; Animals ; Base Sequence ; binding proteins ; Binding Sites ; Biological Transport, Active ; Carrier Proteins - metabolism ; cell division ; Cell Division - physiology ; cell structures ; cleavage furrow ; Concanavalin A ; cortexillin ; Cross-Linking Reagents ; cytochemistry ; cytokinesis ; Dictyostelium ; Dictyostelium - cytology ; Dictyostelium - genetics ; Dictyostelium - metabolism ; Dictyostelium discoideum ; DNA Primers - genetics ; Furrows ; green fluorescent protein ; Membrane Proteins - metabolism ; Microfilament Proteins - chemistry ; Microfilament Proteins - genetics ; Microfilament Proteins - metabolism ; Mutation ; Myosins - metabolism ; Peptide Fragments - chemistry ; Peptide Fragments - genetics ; Peptide Fragments - metabolism ; plasma membrane ; protein targeting ; protein transport ; Protozoan Proteins - chemistry ; Protozoan Proteins - genetics ; Protozoan Proteins - metabolism ; Recombinant Fusion Proteins - chemistry ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; recombinant proteins ; Scyphozoa ; Translocation</subject><ispartof>The EMBO journal, 1999-02, Vol.18 (3), p.586-594</ispartof><rights>European Molecular Biology Organization 1999</rights><rights>Copyright © 1999 European Molecular Biology Organization</rights><rights>Copyright Oxford University Press(England) Feb 01, 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6153-569b2bf83903e6cf8d375145b72e2d868191ed158464eeb4e7ca45c95455e86d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1171151/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1171151/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9927418$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weber, I</creatorcontrib><creatorcontrib>Gerisch, G</creatorcontrib><creatorcontrib>Heizer, C</creatorcontrib><creatorcontrib>Murphy, J</creatorcontrib><creatorcontrib>Badelt, K</creatorcontrib><creatorcontrib>Stock, A</creatorcontrib><creatorcontrib>Schwartz, J.M</creatorcontrib><creatorcontrib>Faix, J</creatorcontrib><title>Cytokinesis mediated through the recruitment of cortexillins into the cleavage furrow</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><addtitle>EMBO J</addtitle><description>The fact that substrate‐anchored
Dictyostelium
cells undergo cytokinesis in the absence of myosin II underscores the importance of other proteins in enabling the cleavage furrow to constrict. Cortexillins, a pair of actin‐bundling proteins, are required for normal cleavage. They are targeted to the incipient furrow in wild‐type and, more prominently, in myosin II‐null cells. No other F‐actin bundling or cross‐linking protein tested is co‐localized. Green fluorescent protein fusions show that the N‐terminal actin‐binding domain of cortexillin I is dispensable and the C‐terminal region is sufficient for translocation to the furrow and the rescue of cytokinesis. Cortexillins are suggested to have a targeting signal for coupling to a myosin II‐independent system that directs transport of membrane proteins to the cleavage furrow.</description><subject>actin</subject><subject>Actins - metabolism</subject><subject>animal proteins</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>binding proteins</subject><subject>Binding Sites</subject><subject>Biological Transport, Active</subject><subject>Carrier Proteins - metabolism</subject><subject>cell division</subject><subject>Cell Division - physiology</subject><subject>cell structures</subject><subject>cleavage furrow</subject><subject>Concanavalin A</subject><subject>cortexillin</subject><subject>Cross-Linking Reagents</subject><subject>cytochemistry</subject><subject>cytokinesis</subject><subject>Dictyostelium</subject><subject>Dictyostelium - cytology</subject><subject>Dictyostelium - genetics</subject><subject>Dictyostelium - metabolism</subject><subject>Dictyostelium discoideum</subject><subject>DNA Primers - genetics</subject><subject>Furrows</subject><subject>green fluorescent protein</subject><subject>Membrane Proteins - metabolism</subject><subject>Microfilament Proteins - chemistry</subject><subject>Microfilament Proteins - genetics</subject><subject>Microfilament Proteins - metabolism</subject><subject>Mutation</subject><subject>Myosins - metabolism</subject><subject>Peptide Fragments - chemistry</subject><subject>Peptide Fragments - genetics</subject><subject>Peptide Fragments - metabolism</subject><subject>plasma membrane</subject><subject>protein targeting</subject><subject>protein transport</subject><subject>Protozoan Proteins - chemistry</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - metabolism</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>recombinant proteins</subject><subject>Scyphozoa</subject><subject>Translocation</subject><issn>0261-4189</issn><issn>1460-2075</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkktv00AUhS0EKqGwZYewWLBzMg_Pa4PURG0BtSABEexGY_s6mdT2lBm7bf490zoKhU00iyvN-e7xsY-T5DVGU4wUnUFbuM0MyymdMsmfJBOcc5QRJNjTZIIIx1mOpXqevAhhgxBiUuCj5EgpIuL9JFkutr27sh0EG9IWKmt6qNJ-7d2wWscJqYfSD7ZvoetTV6el8z3c2aaxXUht17sHqGzA3JgVpPXgvbt9mTyrTRPg1W4eJ8uz0x-Lj9nF1_NPi5OLrOSY0YxxVZCillQhCrysZUUFwzkrBAFSSS6xwlBhJnOeAxQ5iNLkrFQsZwwkr-hx8mH0vR6KGL6MGb1p9LW3rfFb7YzV_yqdXeuVu9EYC4wZjgbvdwbe_R4g9Lq1oYSmMR24IWiumCA05wfB6Mc4RTSC7_4DN27wXfwKGitGGCecRGg6QqV3IXio95Ex0ve16odaNZaa6lhrXHjz-EX3-K7HqItRv7UNbA-46dPL-WfBFM7Vfd7ZuBniUrcC_yjvgSyd6QcP-4f9dcxG3Yb4p-xl4680F7Fg_fPLuZbffs3F4lLpeeTfjnxtnDYrb4NeficIU0SkojyeP7nj4zQ</recordid><startdate>19990201</startdate><enddate>19990201</enddate><creator>Weber, I</creator><creator>Gerisch, G</creator><creator>Heizer, C</creator><creator>Murphy, J</creator><creator>Badelt, K</creator><creator>Stock, A</creator><creator>Schwartz, J.M</creator><creator>Faix, J</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990201</creationdate><title>Cytokinesis mediated through the recruitment of cortexillins into the cleavage furrow</title><author>Weber, I ; Gerisch, G ; Heizer, C ; Murphy, J ; Badelt, K ; Stock, A ; Schwartz, J.M ; Faix, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6153-569b2bf83903e6cf8d375145b72e2d868191ed158464eeb4e7ca45c95455e86d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>actin</topic><topic>Actins - metabolism</topic><topic>animal proteins</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>binding proteins</topic><topic>Binding Sites</topic><topic>Biological Transport, Active</topic><topic>Carrier Proteins - metabolism</topic><topic>cell division</topic><topic>Cell Division - physiology</topic><topic>cell structures</topic><topic>cleavage furrow</topic><topic>Concanavalin A</topic><topic>cortexillin</topic><topic>Cross-Linking Reagents</topic><topic>cytochemistry</topic><topic>cytokinesis</topic><topic>Dictyostelium</topic><topic>Dictyostelium - cytology</topic><topic>Dictyostelium - genetics</topic><topic>Dictyostelium - metabolism</topic><topic>Dictyostelium discoideum</topic><topic>DNA Primers - genetics</topic><topic>Furrows</topic><topic>green fluorescent protein</topic><topic>Membrane Proteins - metabolism</topic><topic>Microfilament Proteins - chemistry</topic><topic>Microfilament Proteins - genetics</topic><topic>Microfilament Proteins - metabolism</topic><topic>Mutation</topic><topic>Myosins - metabolism</topic><topic>Peptide Fragments - chemistry</topic><topic>Peptide Fragments - genetics</topic><topic>Peptide Fragments - metabolism</topic><topic>plasma membrane</topic><topic>protein targeting</topic><topic>protein transport</topic><topic>Protozoan Proteins - chemistry</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - metabolism</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>recombinant proteins</topic><topic>Scyphozoa</topic><topic>Translocation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weber, I</creatorcontrib><creatorcontrib>Gerisch, G</creatorcontrib><creatorcontrib>Heizer, C</creatorcontrib><creatorcontrib>Murphy, J</creatorcontrib><creatorcontrib>Badelt, K</creatorcontrib><creatorcontrib>Stock, A</creatorcontrib><creatorcontrib>Schwartz, J.M</creatorcontrib><creatorcontrib>Faix, J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weber, I</au><au>Gerisch, G</au><au>Heizer, C</au><au>Murphy, J</au><au>Badelt, K</au><au>Stock, A</au><au>Schwartz, J.M</au><au>Faix, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytokinesis mediated through the recruitment of cortexillins into the cleavage furrow</atitle><jtitle>The EMBO journal</jtitle><stitle>EMBO J</stitle><addtitle>EMBO J</addtitle><date>1999-02-01</date><risdate>1999</risdate><volume>18</volume><issue>3</issue><spage>586</spage><epage>594</epage><pages>586-594</pages><issn>0261-4189</issn><issn>1460-2075</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>The fact that substrate‐anchored
Dictyostelium
cells undergo cytokinesis in the absence of myosin II underscores the importance of other proteins in enabling the cleavage furrow to constrict. Cortexillins, a pair of actin‐bundling proteins, are required for normal cleavage. They are targeted to the incipient furrow in wild‐type and, more prominently, in myosin II‐null cells. No other F‐actin bundling or cross‐linking protein tested is co‐localized. Green fluorescent protein fusions show that the N‐terminal actin‐binding domain of cortexillin I is dispensable and the C‐terminal region is sufficient for translocation to the furrow and the rescue of cytokinesis. Cortexillins are suggested to have a targeting signal for coupling to a myosin II‐independent system that directs transport of membrane proteins to the cleavage furrow.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>9927418</pmid><doi>10.1093/emboj/18.3.586</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | actin Actins - metabolism animal proteins Animals Base Sequence binding proteins Binding Sites Biological Transport, Active Carrier Proteins - metabolism cell division Cell Division - physiology cell structures cleavage furrow Concanavalin A cortexillin Cross-Linking Reagents cytochemistry cytokinesis Dictyostelium Dictyostelium - cytology Dictyostelium - genetics Dictyostelium - metabolism Dictyostelium discoideum DNA Primers - genetics Furrows green fluorescent protein Membrane Proteins - metabolism Microfilament Proteins - chemistry Microfilament Proteins - genetics Microfilament Proteins - metabolism Mutation Myosins - metabolism Peptide Fragments - chemistry Peptide Fragments - genetics Peptide Fragments - metabolism plasma membrane protein targeting protein transport Protozoan Proteins - chemistry Protozoan Proteins - genetics Protozoan Proteins - metabolism Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism recombinant proteins Scyphozoa Translocation |
| title | Cytokinesis mediated through the recruitment of cortexillins into the cleavage furrow |
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