A novel capacitative calcium entry channel expressed in excitable cells
In addition to voltage‐gated calcium influx, capacitative calcium entry (CCE) represents a major pathway for calcium entry into the cell. Here we report the structure, expression and functional properties of a novel CCE channel, TRP5. This channel is a member of a new subfamily of mammalian homologu...
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| Veröffentlicht in: | The EMBO journal 1998-08, Vol.17 (15), p.4274-4282 |
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| creator | Philipp, Stephan Hambrecht, Joerg Braslavski, Leonid Schroth, Gregor Freichel, Marc Murakami, Manabu Cavalié, Adolfo Flockerzi, Veit |
| description | In addition to voltage‐gated calcium influx, capacitative calcium entry (CCE) represents a major pathway for calcium entry into the cell. Here we report the structure, expression and functional properties of a novel CCE channel, TRP5. This channel is a member of a new subfamily of mammalian homologues of the
Drosophila
transient receptor potential (TRP) protein, now comprising TRP5 (also CCE2) and the structurally related CCE1 (also TRP4). Like TRP4, TRP5 forms ion channels mainly permeable for Ca
2+
which are not active under resting conditions but can be activated by manoeuvres known to deplete intracellular calcium stores. Accordingly, dialysis of TRP5‐expressing cells with inositol‐(1,4,5)‐trisphosphate evokes inward rectifying currents which reversed polarity at potentials more positive than +30 mV. Ca
2+
store depletion with thapsigargin induced TRP5‐mediated calcium entry dependent on the concentration of extracellular calcium, as seen by dual wavelength fura‐2 fluorescence ratio measurements. TRP5 transcripts are expressed almost exclusively in brain, where they are present in mitral cells of the olfactory bulb, in lateral cerebellar nuclei and, together with TRP4 transcripts, in CA1 pyramidal neurons of the hippocampus, indicating the presence of CCE channels in excitable cells and their participation in neuronal calcium homeostasis. |
| doi_str_mv | 10.1093/emboj/17.15.4274 |
| format | Article |
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Drosophila
transient receptor potential (TRP) protein, now comprising TRP5 (also CCE2) and the structurally related CCE1 (also TRP4). Like TRP4, TRP5 forms ion channels mainly permeable for Ca
2+
which are not active under resting conditions but can be activated by manoeuvres known to deplete intracellular calcium stores. Accordingly, dialysis of TRP5‐expressing cells with inositol‐(1,4,5)‐trisphosphate evokes inward rectifying currents which reversed polarity at potentials more positive than +30 mV. Ca
2+
store depletion with thapsigargin induced TRP5‐mediated calcium entry dependent on the concentration of extracellular calcium, as seen by dual wavelength fura‐2 fluorescence ratio measurements. TRP5 transcripts are expressed almost exclusively in brain, where they are present in mitral cells of the olfactory bulb, in lateral cerebellar nuclei and, together with TRP4 transcripts, in CA1 pyramidal neurons of the hippocampus, indicating the presence of CCE channels in excitable cells and their participation in neuronal calcium homeostasis.</description><identifier>ISSN: 0261-4189</identifier><identifier>ISSN: 1460-2075</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1093/emboj/17.15.4274</identifier><identifier>PMID: 9687496</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Brain - cytology ; Brain - metabolism ; Calcium Channels - biosynthesis ; Calcium Channels - chemistry ; Calcium Channels - genetics ; capacitative calcium entry ; Cation Transport Proteins ; Cattle ; Cloning, Molecular ; DNA, Complementary - isolation & purification ; InsP3 ; Mice ; Molecular Sequence Data ; Organ Specificity - genetics ; Rabbits ; RNA, Messenger - biosynthesis ; store depletion ; store-operated calcium channel ; TRP5 ; TRPC Cation Channels</subject><ispartof>The EMBO journal, 1998-08, Vol.17 (15), p.4274-4282</ispartof><rights>European Molecular Biology Organization 1998</rights><rights>Copyright © 1998 European Molecular Biology Organization</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5528-fd3665ed15bf20b764aa677e7001ab860f89ebe72a94583f70fa1ff0795d6b153</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170761/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170761/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27901,27902,45550,45551,46384,46808,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9687496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Philipp, Stephan</creatorcontrib><creatorcontrib>Hambrecht, Joerg</creatorcontrib><creatorcontrib>Braslavski, Leonid</creatorcontrib><creatorcontrib>Schroth, Gregor</creatorcontrib><creatorcontrib>Freichel, Marc</creatorcontrib><creatorcontrib>Murakami, Manabu</creatorcontrib><creatorcontrib>Cavalié, Adolfo</creatorcontrib><creatorcontrib>Flockerzi, Veit</creatorcontrib><title>A novel capacitative calcium entry channel expressed in excitable cells</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><addtitle>EMBO J</addtitle><description>In addition to voltage‐gated calcium influx, capacitative calcium entry (CCE) represents a major pathway for calcium entry into the cell. Here we report the structure, expression and functional properties of a novel CCE channel, TRP5. This channel is a member of a new subfamily of mammalian homologues of the
Drosophila
transient receptor potential (TRP) protein, now comprising TRP5 (also CCE2) and the structurally related CCE1 (also TRP4). Like TRP4, TRP5 forms ion channels mainly permeable for Ca
2+
which are not active under resting conditions but can be activated by manoeuvres known to deplete intracellular calcium stores. Accordingly, dialysis of TRP5‐expressing cells with inositol‐(1,4,5)‐trisphosphate evokes inward rectifying currents which reversed polarity at potentials more positive than +30 mV. Ca
2+
store depletion with thapsigargin induced TRP5‐mediated calcium entry dependent on the concentration of extracellular calcium, as seen by dual wavelength fura‐2 fluorescence ratio measurements. TRP5 transcripts are expressed almost exclusively in brain, where they are present in mitral cells of the olfactory bulb, in lateral cerebellar nuclei and, together with TRP4 transcripts, in CA1 pyramidal neurons of the hippocampus, indicating the presence of CCE channels in excitable cells and their participation in neuronal calcium homeostasis.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Brain - cytology</subject><subject>Brain - metabolism</subject><subject>Calcium Channels - biosynthesis</subject><subject>Calcium Channels - chemistry</subject><subject>Calcium Channels - genetics</subject><subject>capacitative calcium entry</subject><subject>Cation Transport Proteins</subject><subject>Cattle</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - isolation & purification</subject><subject>InsP3</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity - genetics</subject><subject>Rabbits</subject><subject>RNA, Messenger - biosynthesis</subject><subject>store depletion</subject><subject>store-operated calcium channel</subject><subject>TRP5</subject><subject>TRPC Cation Channels</subject><issn>0261-4189</issn><issn>1460-2075</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKkvhzgUpJ27ZepLYY1-QSinLRykSAnG0nGTSeskmi50s3f8eh6xWcKEnazS_9_xGj7HnwJfAdX5Gm7JfnwEuQSyLDIsHbAGF5GnGUTxkC55JSAtQ-jF7EsKacy4Uwgk70VJhoeWCrc6Trt9Rm1R2ays32MHtKA5t5cZNQt3g90l1a7suInS39RQC1Ynr4jDRZRthatvwlD1qbBvo2eE9Zd_eXn69eJdefV69vzi_SishMpU2dS6loBpE2WS8RFlYKxEJOQdbKskbpakkzKwuhMob5I2FpuGoRS1LEPkpezX7bsdyQ3U1JbSt2Xq3sX5veuvMv5vO3ZqbfmcAkKOEaPDyYOD7nyOFwWxcmE6wHfVjMIrzAnMl7wVBigIhLyLIZ7DyfQiemmMa4GZqyfxpyQAaEGZqKUpe_H3FUXCoJe71vP_lWtrf62cuP73-gEIDZCpqYdaGKOtuyJt1P_oulvK_POmscWGgu-N_1v8wEnMU5vv1yqiPb76A1Giu898PjMDD</recordid><startdate>19980803</startdate><enddate>19980803</enddate><creator>Philipp, Stephan</creator><creator>Hambrecht, Joerg</creator><creator>Braslavski, Leonid</creator><creator>Schroth, Gregor</creator><creator>Freichel, Marc</creator><creator>Murakami, Manabu</creator><creator>Cavalié, Adolfo</creator><creator>Flockerzi, Veit</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980803</creationdate><title>A novel capacitative calcium entry channel expressed in excitable cells</title><author>Philipp, Stephan ; Hambrecht, Joerg ; Braslavski, Leonid ; Schroth, Gregor ; Freichel, Marc ; Murakami, Manabu ; Cavalié, Adolfo ; Flockerzi, Veit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5528-fd3665ed15bf20b764aa677e7001ab860f89ebe72a94583f70fa1ff0795d6b153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Brain - cytology</topic><topic>Brain - metabolism</topic><topic>Calcium Channels - biosynthesis</topic><topic>Calcium Channels - chemistry</topic><topic>Calcium Channels - genetics</topic><topic>capacitative calcium entry</topic><topic>Cation Transport Proteins</topic><topic>Cattle</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - isolation & purification</topic><topic>InsP3</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity - genetics</topic><topic>Rabbits</topic><topic>RNA, Messenger - biosynthesis</topic><topic>store depletion</topic><topic>store-operated calcium channel</topic><topic>TRP5</topic><topic>TRPC Cation Channels</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Philipp, Stephan</creatorcontrib><creatorcontrib>Hambrecht, Joerg</creatorcontrib><creatorcontrib>Braslavski, Leonid</creatorcontrib><creatorcontrib>Schroth, Gregor</creatorcontrib><creatorcontrib>Freichel, Marc</creatorcontrib><creatorcontrib>Murakami, Manabu</creatorcontrib><creatorcontrib>Cavalié, Adolfo</creatorcontrib><creatorcontrib>Flockerzi, Veit</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Philipp, Stephan</au><au>Hambrecht, Joerg</au><au>Braslavski, Leonid</au><au>Schroth, Gregor</au><au>Freichel, Marc</au><au>Murakami, Manabu</au><au>Cavalié, Adolfo</au><au>Flockerzi, Veit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel capacitative calcium entry channel expressed in excitable cells</atitle><jtitle>The EMBO journal</jtitle><stitle>EMBO J</stitle><addtitle>EMBO J</addtitle><date>1998-08-03</date><risdate>1998</risdate><volume>17</volume><issue>15</issue><spage>4274</spage><epage>4282</epage><pages>4274-4282</pages><issn>0261-4189</issn><issn>1460-2075</issn><eissn>1460-2075</eissn><abstract>In addition to voltage‐gated calcium influx, capacitative calcium entry (CCE) represents a major pathway for calcium entry into the cell. Here we report the structure, expression and functional properties of a novel CCE channel, TRP5. This channel is a member of a new subfamily of mammalian homologues of the
Drosophila
transient receptor potential (TRP) protein, now comprising TRP5 (also CCE2) and the structurally related CCE1 (also TRP4). Like TRP4, TRP5 forms ion channels mainly permeable for Ca
2+
which are not active under resting conditions but can be activated by manoeuvres known to deplete intracellular calcium stores. Accordingly, dialysis of TRP5‐expressing cells with inositol‐(1,4,5)‐trisphosphate evokes inward rectifying currents which reversed polarity at potentials more positive than +30 mV. Ca
2+
store depletion with thapsigargin induced TRP5‐mediated calcium entry dependent on the concentration of extracellular calcium, as seen by dual wavelength fura‐2 fluorescence ratio measurements. TRP5 transcripts are expressed almost exclusively in brain, where they are present in mitral cells of the olfactory bulb, in lateral cerebellar nuclei and, together with TRP4 transcripts, in CA1 pyramidal neurons of the hippocampus, indicating the presence of CCE channels in excitable cells and their participation in neuronal calcium homeostasis.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>9687496</pmid><doi>10.1093/emboj/17.15.4274</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Animals Brain - cytology Brain - metabolism Calcium Channels - biosynthesis Calcium Channels - chemistry Calcium Channels - genetics capacitative calcium entry Cation Transport Proteins Cattle Cloning, Molecular DNA, Complementary - isolation & purification InsP3 Mice Molecular Sequence Data Organ Specificity - genetics Rabbits RNA, Messenger - biosynthesis store depletion store-operated calcium channel TRP5 TRPC Cation Channels |
| title | A novel capacitative calcium entry channel expressed in excitable cells |
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