Termination of cytosolic Ca2+ signals: Ca2+ reuptake into intracellular stores is regulated by the free Ca2+ concentration in the store lumen
The mechanism by which agonist‐evoked cytosolic Ca 2+ signals are terminated has been investigated. We measured the Ca 2+ concentration inside the endoplasmic reticulum store of pancreatic acinar cells and monitored the cytoplasmic Ca 2+ concentration by whole‐cell patch‐clamp recording of the Ca 2+...
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| Veröffentlicht in: | The EMBO journal 1998-01, Vol.17 (2), p.435-442 |
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| creator | Mogami, Hideo Tepikin, Alexei V. Petersen, Ole H. |
| description | The mechanism by which agonist‐evoked cytosolic Ca
2+
signals are terminated has been investigated. We measured the Ca
2+
concentration inside the endoplasmic reticulum store of pancreatic acinar cells and monitored the cytoplasmic Ca
2+
concentration by whole‐cell patch‐clamp recording of the Ca
2+
‐sensitive currents. When the cytosolic Ca
2+
concentration was clamped at the resting level by a high concentration of a selective Ca
2+
buffer, acetylcholine evoked the usual depletion of intracellular Ca
2+
stores, but without increasing the Ca
2+
‐sensitive currents. Removal of acetylcholine allowed thapsigargin‐sensitive Ca
2+
reuptake into the stores, and this process stopped when the stores had been loaded to the pre‐stimulation level. The apparent rate of Ca
2+
reuptake decreased steeply with an increase in the Ca
2+
concentration in the store lumen and it is this negative feedback on the Ca
2+
pump that controls the Ca
2+
store content. In the absence of a cytoplasmic Ca
2+
clamp, acetylcholine removal resulted in a rapid return of the elevated cytoplasmic Ca
2+
concentration to the pre‐stimulation resting level, which was attained long before the endoplasmic reticulum Ca
2+
store had been completely refilled. We conclude that control of Ca
2+
reuptake by the Ca
2+
concentration inside the intracellular store allows precise Ca
2+
signal termination without interfering with store refilling. |
| doi_str_mv | 10.1093/emboj/17.2.435 |
| format | Article |
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2+
signals are terminated has been investigated. We measured the Ca
2+
concentration inside the endoplasmic reticulum store of pancreatic acinar cells and monitored the cytoplasmic Ca
2+
concentration by whole‐cell patch‐clamp recording of the Ca
2+
‐sensitive currents. When the cytosolic Ca
2+
concentration was clamped at the resting level by a high concentration of a selective Ca
2+
buffer, acetylcholine evoked the usual depletion of intracellular Ca
2+
stores, but without increasing the Ca
2+
‐sensitive currents. Removal of acetylcholine allowed thapsigargin‐sensitive Ca
2+
reuptake into the stores, and this process stopped when the stores had been loaded to the pre‐stimulation level. The apparent rate of Ca
2+
reuptake decreased steeply with an increase in the Ca
2+
concentration in the store lumen and it is this negative feedback on the Ca
2+
pump that controls the Ca
2+
store content. In the absence of a cytoplasmic Ca
2+
clamp, acetylcholine removal resulted in a rapid return of the elevated cytoplasmic Ca
2+
concentration to the pre‐stimulation resting level, which was attained long before the endoplasmic reticulum Ca
2+
store had been completely refilled. We conclude that control of Ca
2+
reuptake by the Ca
2+
concentration inside the intracellular store allows precise Ca
2+
signal termination without interfering with store refilling.</description><identifier>ISSN: 0261-4189</identifier><identifier>ISSN: 1460-2075</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1093/emboj/17.2.435</identifier><identifier>PMID: 9430635</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Acetylcholine - pharmacology ; Animals ; Calcium - metabolism ; Calcium - physiology ; calcium pump ; Calcium-Transporting ATPases - drug effects ; Calcium-Transporting ATPases - metabolism ; Cell Separation ; cytoplasm ; Cytosol - drug effects ; Cytosol - enzymology ; Cytosol - metabolism ; endoplasmic reticulum ; Intracellular Fluid - drug effects ; Intracellular Fluid - enzymology ; Intracellular Fluid - metabolism ; Mice ; Pancreas - cytology ; Pancreas - enzymology ; Pancreas - metabolism ; Patch-Clamp Techniques ; signal termination ; Signal Transduction - drug effects ; Thapsigargin - pharmacology</subject><ispartof>The EMBO journal, 1998-01, Vol.17 (2), p.435-442</ispartof><rights>European Molecular Biology Organization 1998</rights><rights>Copyright © 1998 European Molecular Biology Organization</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4431-b918c8c6c2364bc4e9d1fec9e0421b9423813256acac7e1113c99901f77ada663</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170394/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170394/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9430635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mogami, Hideo</creatorcontrib><creatorcontrib>Tepikin, Alexei V.</creatorcontrib><creatorcontrib>Petersen, Ole H.</creatorcontrib><title>Termination of cytosolic Ca2+ signals: Ca2+ reuptake into intracellular stores is regulated by the free Ca2+ concentration in the store lumen</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><addtitle>EMBO J</addtitle><description>The mechanism by which agonist‐evoked cytosolic Ca
2+
signals are terminated has been investigated. We measured the Ca
2+
concentration inside the endoplasmic reticulum store of pancreatic acinar cells and monitored the cytoplasmic Ca
2+
concentration by whole‐cell patch‐clamp recording of the Ca
2+
‐sensitive currents. When the cytosolic Ca
2+
concentration was clamped at the resting level by a high concentration of a selective Ca
2+
buffer, acetylcholine evoked the usual depletion of intracellular Ca
2+
stores, but without increasing the Ca
2+
‐sensitive currents. Removal of acetylcholine allowed thapsigargin‐sensitive Ca
2+
reuptake into the stores, and this process stopped when the stores had been loaded to the pre‐stimulation level. The apparent rate of Ca
2+
reuptake decreased steeply with an increase in the Ca
2+
concentration in the store lumen and it is this negative feedback on the Ca
2+
pump that controls the Ca
2+
store content. In the absence of a cytoplasmic Ca
2+
clamp, acetylcholine removal resulted in a rapid return of the elevated cytoplasmic Ca
2+
concentration to the pre‐stimulation resting level, which was attained long before the endoplasmic reticulum Ca
2+
store had been completely refilled. We conclude that control of Ca
2+
reuptake by the Ca
2+
concentration inside the intracellular store allows precise Ca
2+
signal termination without interfering with store refilling.</description><subject>Acetylcholine - pharmacology</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Calcium - physiology</subject><subject>calcium pump</subject><subject>Calcium-Transporting ATPases - drug effects</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Cell Separation</subject><subject>cytoplasm</subject><subject>Cytosol - drug effects</subject><subject>Cytosol - enzymology</subject><subject>Cytosol - metabolism</subject><subject>endoplasmic reticulum</subject><subject>Intracellular Fluid - drug effects</subject><subject>Intracellular Fluid - enzymology</subject><subject>Intracellular Fluid - metabolism</subject><subject>Mice</subject><subject>Pancreas - cytology</subject><subject>Pancreas - enzymology</subject><subject>Pancreas - metabolism</subject><subject>Patch-Clamp Techniques</subject><subject>signal termination</subject><subject>Signal Transduction - drug effects</subject><subject>Thapsigargin - pharmacology</subject><issn>0261-4189</issn><issn>1460-2075</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcuO0zAUtRBoKIUtOySv2KC0fiR2zAJpqGaGxzCwGB47y3FvOu4kcbGTgX4E_4zbVBUsEBtb9nld3YPQU0pmlCg-h7by6zmVMzbLeXEPTWguSMaILO6jCWGCZjkt1UP0KMY1IaQoJT1BJyrnRPBign5dQ2hdZ3rnO-xrbLe9j75xFi8Me4GjW3WmiS_HV4Bh05tbwK7r_e4IxkLTDI0JOPY-QMQuJtYq_fSwxNUW9zeA6wAwGljfWdjJ9nGu28N7JW6GFrrH6EGd4uDJ4Z6iz-dn14s32eXHi7eL08vM5jmnWaVoaUsrLOMir2wOaklrsApIzmilcsZLylkhjDVWAqWUW6UUobWUZmmE4FP0avTdDFULy3GmRm-Ca03Yam-c_hvp3I1e-TtNqSQ8LW-Knh8Mgv8-QOx16-JuF6YDP0QtlRCkZCQRZyPRBh9jgPoYQoneFaj3BWoqNdOpwCR49udoR_qhsYTLEf_hGtj-x02ffXj9ThaKSEGTcj4qYxJ1Kwh67Yewq_ffs2SjwsUefh6zTLjVQnJZ6K9XF1pdvf_G2Selv_DfpQDKWA</recordid><startdate>19980115</startdate><enddate>19980115</enddate><creator>Mogami, Hideo</creator><creator>Tepikin, Alexei V.</creator><creator>Petersen, Ole H.</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980115</creationdate><title>Termination of cytosolic Ca2+ signals: Ca2+ reuptake into intracellular stores is regulated by the free Ca2+ concentration in the store lumen</title><author>Mogami, Hideo ; Tepikin, Alexei V. ; Petersen, Ole H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4431-b918c8c6c2364bc4e9d1fec9e0421b9423813256acac7e1113c99901f77ada663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Acetylcholine - pharmacology</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Calcium - physiology</topic><topic>calcium pump</topic><topic>Calcium-Transporting ATPases - drug effects</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Cell Separation</topic><topic>cytoplasm</topic><topic>Cytosol - drug effects</topic><topic>Cytosol - enzymology</topic><topic>Cytosol - metabolism</topic><topic>endoplasmic reticulum</topic><topic>Intracellular Fluid - drug effects</topic><topic>Intracellular Fluid - enzymology</topic><topic>Intracellular Fluid - metabolism</topic><topic>Mice</topic><topic>Pancreas - cytology</topic><topic>Pancreas - enzymology</topic><topic>Pancreas - metabolism</topic><topic>Patch-Clamp Techniques</topic><topic>signal termination</topic><topic>Signal Transduction - drug effects</topic><topic>Thapsigargin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mogami, Hideo</creatorcontrib><creatorcontrib>Tepikin, Alexei V.</creatorcontrib><creatorcontrib>Petersen, Ole H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mogami, Hideo</au><au>Tepikin, Alexei V.</au><au>Petersen, Ole H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Termination of cytosolic Ca2+ signals: Ca2+ reuptake into intracellular stores is regulated by the free Ca2+ concentration in the store lumen</atitle><jtitle>The EMBO journal</jtitle><stitle>EMBO J</stitle><addtitle>EMBO J</addtitle><date>1998-01-15</date><risdate>1998</risdate><volume>17</volume><issue>2</issue><spage>435</spage><epage>442</epage><pages>435-442</pages><issn>0261-4189</issn><issn>1460-2075</issn><eissn>1460-2075</eissn><abstract>The mechanism by which agonist‐evoked cytosolic Ca
2+
signals are terminated has been investigated. We measured the Ca
2+
concentration inside the endoplasmic reticulum store of pancreatic acinar cells and monitored the cytoplasmic Ca
2+
concentration by whole‐cell patch‐clamp recording of the Ca
2+
‐sensitive currents. When the cytosolic Ca
2+
concentration was clamped at the resting level by a high concentration of a selective Ca
2+
buffer, acetylcholine evoked the usual depletion of intracellular Ca
2+
stores, but without increasing the Ca
2+
‐sensitive currents. Removal of acetylcholine allowed thapsigargin‐sensitive Ca
2+
reuptake into the stores, and this process stopped when the stores had been loaded to the pre‐stimulation level. The apparent rate of Ca
2+
reuptake decreased steeply with an increase in the Ca
2+
concentration in the store lumen and it is this negative feedback on the Ca
2+
pump that controls the Ca
2+
store content. In the absence of a cytoplasmic Ca
2+
clamp, acetylcholine removal resulted in a rapid return of the elevated cytoplasmic Ca
2+
concentration to the pre‐stimulation resting level, which was attained long before the endoplasmic reticulum Ca
2+
store had been completely refilled. We conclude that control of Ca
2+
reuptake by the Ca
2+
concentration inside the intracellular store allows precise Ca
2+
signal termination without interfering with store refilling.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>9430635</pmid><doi>10.1093/emboj/17.2.435</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0261-4189 |
| ispartof | The EMBO journal, 1998-01, Vol.17 (2), p.435-442 |
| issn | 0261-4189 1460-2075 1460-2075 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1170394 |
| source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acetylcholine - pharmacology Animals Calcium - metabolism Calcium - physiology calcium pump Calcium-Transporting ATPases - drug effects Calcium-Transporting ATPases - metabolism Cell Separation cytoplasm Cytosol - drug effects Cytosol - enzymology Cytosol - metabolism endoplasmic reticulum Intracellular Fluid - drug effects Intracellular Fluid - enzymology Intracellular Fluid - metabolism Mice Pancreas - cytology Pancreas - enzymology Pancreas - metabolism Patch-Clamp Techniques signal termination Signal Transduction - drug effects Thapsigargin - pharmacology |
| title | Termination of cytosolic Ca2+ signals: Ca2+ reuptake into intracellular stores is regulated by the free Ca2+ concentration in the store lumen |
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