The Na+-specific interaction between the LysR-type regulator, NhaR, and the nhaA gene encoding the Na+/H+ antiporter of Escherichia coli

We used partially purified NhaR and a highly purified His‐tagged NhaR derivative to identify the cis ‐regulatory sequences of nha A recognized by NhaR and to study the specific effect of Na + on this interaction. Gel retardation assay with DNase I footprinting analysis showed that NhaR binds a regio...

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Veröffentlicht in:	The EMBO journal 1997-10, Vol.16 (19), p.5922-5929
Hauptverfasser:	Carmel, O., Rahav-Manor, O., Dover, N., Shaanan, B., Padan, E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Binding Sites DNA Footprinting DNA Methylation DNA, Bacterial - chemistry DNA, Bacterial - metabolism DNA-Binding Proteins Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins Gene Deletion H+ antiporters Histidine - metabolism Molecular Sequence Data Na+ Na+-specific transcription regulation nhaA-Na+-specific footprint NhaR Promoter Regions, Genetic Sodium - metabolism Sodium-Hydrogen Exchangers - genetics Sodium-Hydrogen Exchangers - metabolism Transcription Factors - chemistry Transcription Factors - metabolism
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