Occupational exposure to diesel engine exhaust and serum levels of microRNAs in a cross‐sectional molecular epidemiology study in China
Diesel engine exhaust (DEE) is an established lung carcinogen, but the biological mechanisms of diesel‐induced lung carcinogenesis are not well understood. MicroRNAs (miRNAs) are small noncoding RNAs that play a potentially important role in regulating gene expression related to lung cancer. We cond...
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| Veröffentlicht in: | Environmental and molecular mutagenesis 2023-03, Vol.64 (3), p.159-166 |
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| creator | Hu, Wei Wong, Jason Y. Y. Dai, Yufei Ren, Dianzhi Blechter, Batel Duan, Huawei Niu, Yong Xu, Jun Fu, Wei Meliefste, Kees Zhou, Baosen Yang, Jufang Ye, Meng Jia, Xiaowei Meng, Tao Bin, Ping Rahman, Mohammad L. Dean Hosgood, H. Vermeulen, Roel C. Silverman, Debra T. Zheng, Yuxin Lan, Qing Rothman, Nathaniel |
| description | Diesel engine exhaust (DEE) is an established lung carcinogen, but the biological mechanisms of diesel‐induced lung carcinogenesis are not well understood. MicroRNAs (miRNAs) are small noncoding RNAs that play a potentially important role in regulating gene expression related to lung cancer. We conducted a cross‐sectional molecular epidemiology study to evaluate whether serum levels of miRNAs are altered in healthy workers occupationally exposed to DEE compared to unexposed controls. We conducted a two‐stage study, first measuring 405 miRNAs in a pilot study of six DEE‐exposed workers exposed and six controls. In the second stage, 44 selected miRNAs were measured using the Fireplex circulating miRNA assay that profiles miRNAs directly from biofluids of 45 workers exposed to a range of DEE (Elemental Carbon (EC), median, range: 47.7, 6.1–79.7 μg/m3) and 46 controls. The relationship between exposure to DEE and EC with miRNA levels was analyzed using linear regression adjusted for potential confounders. Serum levels of four miRNAs were significantly lower (miR‐191‐5p, miR‐93‐5p, miR‐423‐3p, miR‐122‐5p) and one miRNA was significantly higher (miR‐92a‐3p) in DEE exposed workers compared to controls. Of these miRNAs, miR‐191‐5p (ptrend = .001, FDR = 0.04) and miR‐93‐5p (ptrend = .009, FDR = 0.18) showed evidence of an inverse exposure–response with increasing EC levels. Our findings suggest that occupational exposure to DEE may affect circulating miRNAs implicated in biological processes related to carcinogenesis, including immune function. |
| doi_str_mv | 10.1002/em.22533 |
| format | Article |
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Y. ; Dai, Yufei ; Ren, Dianzhi ; Blechter, Batel ; Duan, Huawei ; Niu, Yong ; Xu, Jun ; Fu, Wei ; Meliefste, Kees ; Zhou, Baosen ; Yang, Jufang ; Ye, Meng ; Jia, Xiaowei ; Meng, Tao ; Bin, Ping ; Rahman, Mohammad L. ; Dean Hosgood, H. ; Vermeulen, Roel C. ; Silverman, Debra T. ; Zheng, Yuxin ; Lan, Qing ; Rothman, Nathaniel</creator><creatorcontrib>Hu, Wei ; Wong, Jason Y. Y. ; Dai, Yufei ; Ren, Dianzhi ; Blechter, Batel ; Duan, Huawei ; Niu, Yong ; Xu, Jun ; Fu, Wei ; Meliefste, Kees ; Zhou, Baosen ; Yang, Jufang ; Ye, Meng ; Jia, Xiaowei ; Meng, Tao ; Bin, Ping ; Rahman, Mohammad L. ; Dean Hosgood, H. ; Vermeulen, Roel C. ; Silverman, Debra T. ; Zheng, Yuxin ; Lan, Qing ; Rothman, Nathaniel</creatorcontrib><description>Diesel engine exhaust (DEE) is an established lung carcinogen, but the biological mechanisms of diesel‐induced lung carcinogenesis are not well understood. MicroRNAs (miRNAs) are small noncoding RNAs that play a potentially important role in regulating gene expression related to lung cancer. We conducted a cross‐sectional molecular epidemiology study to evaluate whether serum levels of miRNAs are altered in healthy workers occupationally exposed to DEE compared to unexposed controls. We conducted a two‐stage study, first measuring 405 miRNAs in a pilot study of six DEE‐exposed workers exposed and six controls. In the second stage, 44 selected miRNAs were measured using the Fireplex circulating miRNA assay that profiles miRNAs directly from biofluids of 45 workers exposed to a range of DEE (Elemental Carbon (EC), median, range: 47.7, 6.1–79.7 μg/m3) and 46 controls. The relationship between exposure to DEE and EC with miRNA levels was analyzed using linear regression adjusted for potential confounders. Serum levels of four miRNAs were significantly lower (miR‐191‐5p, miR‐93‐5p, miR‐423‐3p, miR‐122‐5p) and one miRNA was significantly higher (miR‐92a‐3p) in DEE exposed workers compared to controls. Of these miRNAs, miR‐191‐5p (ptrend = .001, FDR = 0.04) and miR‐93‐5p (ptrend = .009, FDR = 0.18) showed evidence of an inverse exposure–response with increasing EC levels. Our findings suggest that occupational exposure to DEE may affect circulating miRNAs implicated in biological processes related to carcinogenesis, including immune function.</description><identifier>ISSN: 0893-6692</identifier><identifier>EISSN: 1098-2280</identifier><identifier>DOI: 10.1002/em.22533</identifier><identifier>PMID: 36762959</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Air Pollutants, Occupational - analysis ; Air Pollutants, Occupational - toxicity ; Biological activity ; blood serum ; carbon ; Carcinogenesis ; Carcinogens ; China ; Cross-Sectional Studies ; Diesel ; diesel engine exhaust ; Diesel engines ; Epidemiology ; Exhaust emissions ; Exposure ; Gene expression ; Humans ; Immune response ; Lung cancer ; lung neoplasms ; lungs ; microRNA ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; Molecular Epidemiology ; mutagenesis ; Occupational exposure ; Occupational Exposure - adverse effects ; Occupational Exposure - analysis ; Occupational health ; Pilot Projects ; regression analysis ; Serum levels ; Vehicle Emissions - analysis ; Vehicle Emissions - toxicity</subject><ispartof>Environmental and molecular mutagenesis, 2023-03, Vol.64 (3), p.159-166</ispartof><rights>2023 Environmental Mutagen Society. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.</rights><rights>2023 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4383-55a01e8c1e41e3f25c4b65f38730fe9cfc1ce35650578a2af4935a265a6d05163</citedby><cites>FETCH-LOGICAL-c4383-55a01e8c1e41e3f25c4b65f38730fe9cfc1ce35650578a2af4935a265a6d05163</cites><orcidid>0000-0002-4138-7905 ; 0000-0003-2820-2133</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fem.22533$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fem.22533$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36762959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Wei</creatorcontrib><creatorcontrib>Wong, Jason Y. Y.</creatorcontrib><creatorcontrib>Dai, Yufei</creatorcontrib><creatorcontrib>Ren, Dianzhi</creatorcontrib><creatorcontrib>Blechter, Batel</creatorcontrib><creatorcontrib>Duan, Huawei</creatorcontrib><creatorcontrib>Niu, Yong</creatorcontrib><creatorcontrib>Xu, Jun</creatorcontrib><creatorcontrib>Fu, Wei</creatorcontrib><creatorcontrib>Meliefste, Kees</creatorcontrib><creatorcontrib>Zhou, Baosen</creatorcontrib><creatorcontrib>Yang, Jufang</creatorcontrib><creatorcontrib>Ye, Meng</creatorcontrib><creatorcontrib>Jia, Xiaowei</creatorcontrib><creatorcontrib>Meng, Tao</creatorcontrib><creatorcontrib>Bin, Ping</creatorcontrib><creatorcontrib>Rahman, Mohammad L.</creatorcontrib><creatorcontrib>Dean Hosgood, H.</creatorcontrib><creatorcontrib>Vermeulen, Roel C.</creatorcontrib><creatorcontrib>Silverman, Debra T.</creatorcontrib><creatorcontrib>Zheng, Yuxin</creatorcontrib><creatorcontrib>Lan, Qing</creatorcontrib><creatorcontrib>Rothman, Nathaniel</creatorcontrib><title>Occupational exposure to diesel engine exhaust and serum levels of microRNAs in a cross‐sectional molecular epidemiology study in China</title><title>Environmental and molecular mutagenesis</title><addtitle>Environ Mol Mutagen</addtitle><description>Diesel engine exhaust (DEE) is an established lung carcinogen, but the biological mechanisms of diesel‐induced lung carcinogenesis are not well understood. MicroRNAs (miRNAs) are small noncoding RNAs that play a potentially important role in regulating gene expression related to lung cancer. We conducted a cross‐sectional molecular epidemiology study to evaluate whether serum levels of miRNAs are altered in healthy workers occupationally exposed to DEE compared to unexposed controls. We conducted a two‐stage study, first measuring 405 miRNAs in a pilot study of six DEE‐exposed workers exposed and six controls. In the second stage, 44 selected miRNAs were measured using the Fireplex circulating miRNA assay that profiles miRNAs directly from biofluids of 45 workers exposed to a range of DEE (Elemental Carbon (EC), median, range: 47.7, 6.1–79.7 μg/m3) and 46 controls. The relationship between exposure to DEE and EC with miRNA levels was analyzed using linear regression adjusted for potential confounders. Serum levels of four miRNAs were significantly lower (miR‐191‐5p, miR‐93‐5p, miR‐423‐3p, miR‐122‐5p) and one miRNA was significantly higher (miR‐92a‐3p) in DEE exposed workers compared to controls. Of these miRNAs, miR‐191‐5p (ptrend = .001, FDR = 0.04) and miR‐93‐5p (ptrend = .009, FDR = 0.18) showed evidence of an inverse exposure–response with increasing EC levels. Our findings suggest that occupational exposure to DEE may affect circulating miRNAs implicated in biological processes related to carcinogenesis, including immune function.</description><subject>Air Pollutants, Occupational - analysis</subject><subject>Air Pollutants, Occupational - toxicity</subject><subject>Biological activity</subject><subject>blood serum</subject><subject>carbon</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>China</subject><subject>Cross-Sectional Studies</subject><subject>Diesel</subject><subject>diesel engine exhaust</subject><subject>Diesel engines</subject><subject>Epidemiology</subject><subject>Exhaust emissions</subject><subject>Exposure</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Immune response</subject><subject>Lung cancer</subject><subject>lung neoplasms</subject><subject>lungs</subject><subject>microRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>Molecular Epidemiology</subject><subject>mutagenesis</subject><subject>Occupational exposure</subject><subject>Occupational Exposure - adverse effects</subject><subject>Occupational Exposure - analysis</subject><subject>Occupational health</subject><subject>Pilot Projects</subject><subject>regression analysis</subject><subject>Serum levels</subject><subject>Vehicle Emissions - analysis</subject><subject>Vehicle Emissions - toxicity</subject><issn>0893-6692</issn><issn>1098-2280</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1DAQgC0EoktB4gmQJS5cUvwTO_EJVasWkAqVEJwt15nsurLjYMcte-uVG8_Ik5DtLuVHQpys8XzzaTwehJ5SckQJYS8hHDEmOL-HFpSotmKsJffRgrSKV1IqdoAe5XxJCKW1Yg_RAZeNZEqoBfp6bm0ZzeTiYDyGL2PMJQGeIu4cZJivhpUbYM6sTckTNkOHM6QSsIcr8BnHHgdnU_zw_jhjN2CD5yDn7zffMti9NkQPtniTMIyug-Cij6sNzlPpNtua5doN5jF60Buf4cn-PESfTk8-Lt9UZ-ev3y6Pzypb85ZXQhhCobUUagq8Z8LWF1L0vG046UHZ3lILXEhBRNMaZvpacWGYFEZ2RFDJD9GrnXcsFwE6C8OUjNdjcsGkjY7G6T8zg1vrVbzSlDaE0XpreLE3pPi5QJ50cNmC92aAWLJmLaWqoZLQ_6NNIyTjqmUz-vwv9DKWNI9vS6nZKBoqfglvp5ygv2ucEr3dBQ1B3-7CjD77_aF34M_Pn4FqB1w7D5t_ivTJu53wB5pvv4w</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Hu, Wei</creator><creator>Wong, Jason Y. 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Y. ; Dai, Yufei ; Ren, Dianzhi ; Blechter, Batel ; Duan, Huawei ; Niu, Yong ; Xu, Jun ; Fu, Wei ; Meliefste, Kees ; Zhou, Baosen ; Yang, Jufang ; Ye, Meng ; Jia, Xiaowei ; Meng, Tao ; Bin, Ping ; Rahman, Mohammad L. ; Dean Hosgood, H. ; Vermeulen, Roel C. ; Silverman, Debra T. ; Zheng, Yuxin ; Lan, Qing ; Rothman, Nathaniel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4383-55a01e8c1e41e3f25c4b65f38730fe9cfc1ce35650578a2af4935a265a6d05163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Air Pollutants, Occupational - analysis</topic><topic>Air Pollutants, Occupational - toxicity</topic><topic>Biological activity</topic><topic>blood serum</topic><topic>carbon</topic><topic>Carcinogenesis</topic><topic>Carcinogens</topic><topic>China</topic><topic>Cross-Sectional Studies</topic><topic>Diesel</topic><topic>diesel engine exhaust</topic><topic>Diesel engines</topic><topic>Epidemiology</topic><topic>Exhaust emissions</topic><topic>Exposure</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Immune response</topic><topic>Lung cancer</topic><topic>lung neoplasms</topic><topic>lungs</topic><topic>microRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>Molecular Epidemiology</topic><topic>mutagenesis</topic><topic>Occupational exposure</topic><topic>Occupational Exposure - adverse effects</topic><topic>Occupational Exposure - analysis</topic><topic>Occupational health</topic><topic>Pilot Projects</topic><topic>regression analysis</topic><topic>Serum levels</topic><topic>Vehicle Emissions - analysis</topic><topic>Vehicle Emissions - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Wei</creatorcontrib><creatorcontrib>Wong, Jason Y. 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Y.</au><au>Dai, Yufei</au><au>Ren, Dianzhi</au><au>Blechter, Batel</au><au>Duan, Huawei</au><au>Niu, Yong</au><au>Xu, Jun</au><au>Fu, Wei</au><au>Meliefste, Kees</au><au>Zhou, Baosen</au><au>Yang, Jufang</au><au>Ye, Meng</au><au>Jia, Xiaowei</au><au>Meng, Tao</au><au>Bin, Ping</au><au>Rahman, Mohammad L.</au><au>Dean Hosgood, H.</au><au>Vermeulen, Roel C.</au><au>Silverman, Debra T.</au><au>Zheng, Yuxin</au><au>Lan, Qing</au><au>Rothman, Nathaniel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occupational exposure to diesel engine exhaust and serum levels of microRNAs in a cross‐sectional molecular epidemiology study in China</atitle><jtitle>Environmental and molecular mutagenesis</jtitle><addtitle>Environ Mol Mutagen</addtitle><date>2023-03</date><risdate>2023</risdate><volume>64</volume><issue>3</issue><spage>159</spage><epage>166</epage><pages>159-166</pages><issn>0893-6692</issn><eissn>1098-2280</eissn><abstract>Diesel engine exhaust (DEE) is an established lung carcinogen, but the biological mechanisms of diesel‐induced lung carcinogenesis are not well understood. MicroRNAs (miRNAs) are small noncoding RNAs that play a potentially important role in regulating gene expression related to lung cancer. We conducted a cross‐sectional molecular epidemiology study to evaluate whether serum levels of miRNAs are altered in healthy workers occupationally exposed to DEE compared to unexposed controls. We conducted a two‐stage study, first measuring 405 miRNAs in a pilot study of six DEE‐exposed workers exposed and six controls. In the second stage, 44 selected miRNAs were measured using the Fireplex circulating miRNA assay that profiles miRNAs directly from biofluids of 45 workers exposed to a range of DEE (Elemental Carbon (EC), median, range: 47.7, 6.1–79.7 μg/m3) and 46 controls. The relationship between exposure to DEE and EC with miRNA levels was analyzed using linear regression adjusted for potential confounders. Serum levels of four miRNAs were significantly lower (miR‐191‐5p, miR‐93‐5p, miR‐423‐3p, miR‐122‐5p) and one miRNA was significantly higher (miR‐92a‐3p) in DEE exposed workers compared to controls. Of these miRNAs, miR‐191‐5p (ptrend = .001, FDR = 0.04) and miR‐93‐5p (ptrend = .009, FDR = 0.18) showed evidence of an inverse exposure–response with increasing EC levels. Our findings suggest that occupational exposure to DEE may affect circulating miRNAs implicated in biological processes related to carcinogenesis, including immune function.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>36762959</pmid><doi>10.1002/em.22533</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4138-7905</orcidid><orcidid>https://orcid.org/0000-0003-2820-2133</orcidid></addata></record> |
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| ispartof | Environmental and molecular mutagenesis, 2023-03, Vol.64 (3), p.159-166 |
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| subjects | Air Pollutants, Occupational - analysis Air Pollutants, Occupational - toxicity Biological activity blood serum carbon Carcinogenesis Carcinogens China Cross-Sectional Studies Diesel diesel engine exhaust Diesel engines Epidemiology Exhaust emissions Exposure Gene expression Humans Immune response Lung cancer lung neoplasms lungs microRNA MicroRNAs MicroRNAs - genetics miRNA Molecular Epidemiology mutagenesis Occupational exposure Occupational Exposure - adverse effects Occupational Exposure - analysis Occupational health Pilot Projects regression analysis Serum levels Vehicle Emissions - analysis Vehicle Emissions - toxicity |
| title | Occupational exposure to diesel engine exhaust and serum levels of microRNAs in a cross‐sectional molecular epidemiology study in China |
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