Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite
Spinal fusion surgery remains a significant challenge due to limitations in current bone graft materials, particularly in terms of bioactivity, integration, and safety. This study presents an innovative approach using an injectable hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) hydrogel combined w...
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| Veröffentlicht in: | Materials today bio 2025-02, Vol.30, p.101379, Article 101379 |
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| creator | Lee, Hye Yeong An, Seong Bae Hwang, Sae Yeon Hwang, Gwang Yong Lee, Hye-Lan Park, Hyun Jung Shin, Joongkyum Kim, Keung Nyun Wee, Sung Won Yoon, Sol Lip Ha, Yoon |
| description | Spinal fusion surgery remains a significant challenge due to limitations in current bone graft materials, particularly in terms of bioactivity, integration, and safety. This study presents an innovative approach using an injectable hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) hydrogel combined with stromal vascular fraction (SVF) and low-dose recombinant human BMP-2 (rhBMP-2) to enhance osteodifferentiation and angiogenesis. Through a series of in vitro studies and preclinical models involving rats and minipigs, we demonstrated that the hydrogel system enables the sustained release of rhBMP-2, resulting in significantly improved bone density and integration, alongside reduced inflammatory responses. The combination of rhBMP-2 and SVF in this injectable formulation yielded superior spinal fusion outcomes, with enhanced mechanical properties and increased bone mass in both small and large animal models. These findings suggest that this strategy offers a promising and safer alternative for spinal fusion, with strong potential for clinical application.
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| doi_str_mv | 10.1016/j.mtbio.2024.101379 |
| format | Article |
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[Display omitted]</description><identifier>ISSN: 2590-0064</identifier><identifier>EISSN: 2590-0064</identifier><identifier>DOI: 10.1016/j.mtbio.2024.101379</identifier><identifier>PMID: 39759847</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Full Length ; Injectable putty ; Low-dose recombinant human BMP-2 ; Osteodifferentiation and angiogenesis ; Preclinical models ; Spinal fusion surgery ; Stromal vascular fraction ; Synergy</subject><ispartof>Materials today bio, 2025-02, Vol.30, p.101379, Article 101379</ispartof><rights>2024</rights><rights>2024 Published by Elsevier Ltd.</rights><rights>2024 Published by Elsevier Ltd. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c406t-c3378f3a79a928e1fc90dc20fffe662d1e16e2c19e03f8e2949a46bea9f20ca03</cites><orcidid>0000-0002-6246-8042 ; 0000-0001-7274-391X ; 0000-0002-1907-3766 ; 0000-0002-7756-4379 ; 0000-0002-2935-4975</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699625/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699625/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39759847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Hye Yeong</creatorcontrib><creatorcontrib>An, Seong Bae</creatorcontrib><creatorcontrib>Hwang, Sae Yeon</creatorcontrib><creatorcontrib>Hwang, Gwang Yong</creatorcontrib><creatorcontrib>Lee, Hye-Lan</creatorcontrib><creatorcontrib>Park, Hyun Jung</creatorcontrib><creatorcontrib>Shin, Joongkyum</creatorcontrib><creatorcontrib>Kim, Keung Nyun</creatorcontrib><creatorcontrib>Wee, Sung Won</creatorcontrib><creatorcontrib>Yoon, Sol Lip</creatorcontrib><creatorcontrib>Ha, Yoon</creatorcontrib><title>Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite</title><title>Materials today bio</title><addtitle>Mater Today Bio</addtitle><description>Spinal fusion surgery remains a significant challenge due to limitations in current bone graft materials, particularly in terms of bioactivity, integration, and safety. This study presents an innovative approach using an injectable hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) hydrogel combined with stromal vascular fraction (SVF) and low-dose recombinant human BMP-2 (rhBMP-2) to enhance osteodifferentiation and angiogenesis. Through a series of in vitro studies and preclinical models involving rats and minipigs, we demonstrated that the hydrogel system enables the sustained release of rhBMP-2, resulting in significantly improved bone density and integration, alongside reduced inflammatory responses. The combination of rhBMP-2 and SVF in this injectable formulation yielded superior spinal fusion outcomes, with enhanced mechanical properties and increased bone mass in both small and large animal models. These findings suggest that this strategy offers a promising and safer alternative for spinal fusion, with strong potential for clinical application.
[Display omitted]</description><subject>Full Length</subject><subject>Injectable putty</subject><subject>Low-dose recombinant human BMP-2</subject><subject>Osteodifferentiation and angiogenesis</subject><subject>Preclinical models</subject><subject>Spinal fusion surgery</subject><subject>Stromal vascular fraction</subject><subject>Synergy</subject><issn>2590-0064</issn><issn>2590-0064</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9kstuFDEQRVsIRKKQL0BCXrLpwY9-eYEQRDwiBYEErK1qd3nGI7c92N0TzZfwu3jSQ5Rs2NhW1a1TVtUtipeMrhhlzZvtapx6G1ac8uoYEa18UpzzWtKS0qZ6-uB9VlymtKWU8lZWlMrnxZmQbS27qj0v_vw4eIxrmyarCfoNeI0j-okEQ9LOenDEzMkGT6wnu4jaWW91jo5hQJdIzvk1ceG2HEJCEjcfvn4vOQE_kDTFMGblHpKeHURiIujphILjuUU9Qe-QbA5DDGt0RIdxF5Kd8EXxzIBLeHm6L4pfnz7-vPpS3nz7fH31_qbUFW2mUgvRdkZAK0HyDpnRkg6aU2MMNg0fGLIGuWYSqTAdcllJqJoeQRpONVBxUVwv3CHAVu2iHSEeVACr7gIhrhXEPBuHqm8HI7SWrDG64sg7LkXNDDMcoRUgM-vdwtrN_YiDzmOM4B5BH2e83ah12CvGGikbXmfC6xMhht8zpkmNNml0DjyGOSnBatZ1lNc8S8Ui1TGkFNHc92FUHS2iturOIupoEbVYJFe9evjF-5p_hsiCt4sgLxf3FqNK2mI2xWDz8qc8FfvfBn8BHkzSWw</recordid><startdate>20250201</startdate><enddate>20250201</enddate><creator>Lee, Hye Yeong</creator><creator>An, Seong Bae</creator><creator>Hwang, Sae Yeon</creator><creator>Hwang, Gwang Yong</creator><creator>Lee, Hye-Lan</creator><creator>Park, Hyun Jung</creator><creator>Shin, Joongkyum</creator><creator>Kim, Keung Nyun</creator><creator>Wee, Sung Won</creator><creator>Yoon, Sol Lip</creator><creator>Ha, Yoon</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6246-8042</orcidid><orcidid>https://orcid.org/0000-0001-7274-391X</orcidid><orcidid>https://orcid.org/0000-0002-1907-3766</orcidid><orcidid>https://orcid.org/0000-0002-7756-4379</orcidid><orcidid>https://orcid.org/0000-0002-2935-4975</orcidid></search><sort><creationdate>20250201</creationdate><title>Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite</title><author>Lee, Hye Yeong ; An, Seong Bae ; Hwang, Sae Yeon ; Hwang, Gwang Yong ; Lee, Hye-Lan ; Park, Hyun Jung ; Shin, Joongkyum ; Kim, Keung Nyun ; Wee, Sung Won ; Yoon, Sol Lip ; Ha, Yoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-c3378f3a79a928e1fc90dc20fffe662d1e16e2c19e03f8e2949a46bea9f20ca03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Full Length</topic><topic>Injectable putty</topic><topic>Low-dose recombinant human BMP-2</topic><topic>Osteodifferentiation and angiogenesis</topic><topic>Preclinical models</topic><topic>Spinal fusion surgery</topic><topic>Stromal vascular fraction</topic><topic>Synergy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Hye Yeong</creatorcontrib><creatorcontrib>An, Seong Bae</creatorcontrib><creatorcontrib>Hwang, Sae Yeon</creatorcontrib><creatorcontrib>Hwang, Gwang Yong</creatorcontrib><creatorcontrib>Lee, Hye-Lan</creatorcontrib><creatorcontrib>Park, Hyun Jung</creatorcontrib><creatorcontrib>Shin, Joongkyum</creatorcontrib><creatorcontrib>Kim, Keung Nyun</creatorcontrib><creatorcontrib>Wee, Sung Won</creatorcontrib><creatorcontrib>Yoon, Sol Lip</creatorcontrib><creatorcontrib>Ha, Yoon</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Materials today bio</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Hye Yeong</au><au>An, Seong Bae</au><au>Hwang, Sae Yeon</au><au>Hwang, Gwang Yong</au><au>Lee, Hye-Lan</au><au>Park, Hyun Jung</au><au>Shin, Joongkyum</au><au>Kim, Keung Nyun</au><au>Wee, Sung Won</au><au>Yoon, Sol Lip</au><au>Ha, Yoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite</atitle><jtitle>Materials today bio</jtitle><addtitle>Mater Today Bio</addtitle><date>2025-02-01</date><risdate>2025</risdate><volume>30</volume><spage>101379</spage><pages>101379-</pages><artnum>101379</artnum><issn>2590-0064</issn><eissn>2590-0064</eissn><abstract>Spinal fusion surgery remains a significant challenge due to limitations in current bone graft materials, particularly in terms of bioactivity, integration, and safety. This study presents an innovative approach using an injectable hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) hydrogel combined with stromal vascular fraction (SVF) and low-dose recombinant human BMP-2 (rhBMP-2) to enhance osteodifferentiation and angiogenesis. Through a series of in vitro studies and preclinical models involving rats and minipigs, we demonstrated that the hydrogel system enables the sustained release of rhBMP-2, resulting in significantly improved bone density and integration, alongside reduced inflammatory responses. The combination of rhBMP-2 and SVF in this injectable formulation yielded superior spinal fusion outcomes, with enhanced mechanical properties and increased bone mass in both small and large animal models. These findings suggest that this strategy offers a promising and safer alternative for spinal fusion, with strong potential for clinical application.
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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Full Length Injectable putty Low-dose recombinant human BMP-2 Osteodifferentiation and angiogenesis Preclinical models Spinal fusion surgery Stromal vascular fraction Synergy |
| title | Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite |
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