A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface
Murine cytomegalovirus (MCMV) interferes with antigen presentation by means of retaining major histocompatibility complex (MHC) class I molecules in the endoplasmic reticulum (ER). Here we identify and characterize an MCMV‐encoded glycoprotein, gp34, which tightly associates with properly conformed...
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| Veröffentlicht in: | The EMBO journal 1997-02, Vol.16 (4), p.685-694 |
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| creator | Kleijnen, Maurits F. Huppa, Johannes B. Lucin, Pero Mukherjee, Siddhartha Farrell, Helen Campbell, Ann E. Koszinowski, Ulrich H. Hill, Ann B. Ploegh, Hidde L. |
| description | Murine cytomegalovirus (MCMV) interferes with antigen presentation by means of retaining major histocompatibility complex (MHC) class I molecules in the endoplasmic reticulum (ER). Here we identify and characterize an MCMV‐encoded glycoprotein, gp34, which tightly associates with properly conformed MHC class I molecules in the ER. Gp34 is synthesized in large quantities during MCMV infection and it leaves the ER only in association with MHC class I complexes. Many but not all class I molecules are retained in the ER during the early phase of MCMV infection, and we observe an inverse correlation between amounts of gp34 synthesized during the course of infection and class I retention. An MCMV deletion mutant lacking several genes, including the gene encoding gp34, shows increased class I retention. Thus, MCMV gp34 may counteract class I retention, perhaps to decrease susceptibility of infected cells to recognition by natural killer cells. |
| doi_str_mv | 10.1093/emboj/16.4.685 |
| format | Article |
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Here we identify and characterize an MCMV‐encoded glycoprotein, gp34, which tightly associates with properly conformed MHC class I molecules in the ER. Gp34 is synthesized in large quantities during MCMV infection and it leaves the ER only in association with MHC class I complexes. Many but not all class I molecules are retained in the ER during the early phase of MCMV infection, and we observe an inverse correlation between amounts of gp34 synthesized during the course of infection and class I retention. An MCMV deletion mutant lacking several genes, including the gene encoding gp34, shows increased class I retention. Thus, MCMV gp34 may counteract class I retention, perhaps to decrease susceptibility of infected cells to recognition by natural killer cells.</description><identifier>ISSN: 0261-4189</identifier><identifier>ISSN: 1460-2075</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1093/emboj/16.4.685</identifier><identifier>PMID: 9049298</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Viral ; antigen presentation ; Brefeldin A ; Carrier Proteins - biosynthesis ; Carrier Proteins - chemistry ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cells, Cultured ; Cloning, Molecular ; Cyclopentanes - pharmacology ; Endoplasmic Reticulum - metabolism ; Endoplasmic Reticulum - virology ; ER retention ; Fibroblasts - virology ; Genes, Viral - genetics ; Glycoproteins - biosynthesis ; Glycoproteins - chemistry ; Glycoproteins - genetics ; Glycoproteins - metabolism ; gp34 ; Hexosaminidases ; Histocompatibility Antigens Class I - chemistry ; Histocompatibility Antigens Class I - metabolism ; Membrane Glycoproteins - biosynthesis ; Membrane Glycoproteins - chemistry ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; MHC class I ; Mice ; Molecular Sequence Data ; Molecular Weight ; murine cytomegalovirus ; Muromegalovirus - genetics ; Muromegalovirus - immunology ; Protein Binding ; Protein Folding ; Protein Synthesis Inhibitors - pharmacology ; Sequence Deletion ; Viral Proteins</subject><ispartof>The EMBO journal, 1997-02, Vol.16 (4), p.685-694</ispartof><rights>European Molecular Biology Organization 1997</rights><rights>Copyright © 1997 European Molecular Biology Organization</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5713-e37946b232acccece10c5564c9fa07e0bd69d94c945fa2fd6a918c7cb7b38ce93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1169670/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1169670/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27903,27904,45553,45554,46387,46811,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9049298$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kleijnen, Maurits F.</creatorcontrib><creatorcontrib>Huppa, Johannes B.</creatorcontrib><creatorcontrib>Lucin, Pero</creatorcontrib><creatorcontrib>Mukherjee, Siddhartha</creatorcontrib><creatorcontrib>Farrell, Helen</creatorcontrib><creatorcontrib>Campbell, Ann E.</creatorcontrib><creatorcontrib>Koszinowski, Ulrich H.</creatorcontrib><creatorcontrib>Hill, Ann B.</creatorcontrib><creatorcontrib>Ploegh, Hidde L.</creatorcontrib><title>A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><addtitle>EMBO J</addtitle><description>Murine cytomegalovirus (MCMV) interferes with antigen presentation by means of retaining major histocompatibility complex (MHC) class I molecules in the endoplasmic reticulum (ER). Here we identify and characterize an MCMV‐encoded glycoprotein, gp34, which tightly associates with properly conformed MHC class I molecules in the ER. Gp34 is synthesized in large quantities during MCMV infection and it leaves the ER only in association with MHC class I complexes. Many but not all class I molecules are retained in the ER during the early phase of MCMV infection, and we observe an inverse correlation between amounts of gp34 synthesized during the course of infection and class I retention. An MCMV deletion mutant lacking several genes, including the gene encoding gp34, shows increased class I retention. Thus, MCMV gp34 may counteract class I retention, perhaps to decrease susceptibility of infected cells to recognition by natural killer cells.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Viral</subject><subject>antigen presentation</subject><subject>Brefeldin A</subject><subject>Carrier Proteins - biosynthesis</subject><subject>Carrier Proteins - chemistry</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>Cyclopentanes - pharmacology</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Endoplasmic Reticulum - virology</subject><subject>ER retention</subject><subject>Fibroblasts - virology</subject><subject>Genes, Viral - genetics</subject><subject>Glycoproteins - biosynthesis</subject><subject>Glycoproteins - chemistry</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - metabolism</subject><subject>gp34</subject><subject>Hexosaminidases</subject><subject>Histocompatibility Antigens Class I - chemistry</subject><subject>Histocompatibility Antigens Class I - metabolism</subject><subject>Membrane Glycoproteins - biosynthesis</subject><subject>Membrane Glycoproteins - chemistry</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>MHC class I</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>murine cytomegalovirus</subject><subject>Muromegalovirus - genetics</subject><subject>Muromegalovirus - immunology</subject><subject>Protein Binding</subject><subject>Protein Folding</subject><subject>Protein Synthesis Inhibitors - pharmacology</subject><subject>Sequence Deletion</subject><subject>Viral Proteins</subject><issn>0261-4189</issn><issn>1460-2075</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUktvEzEQXiFQKYUrNySfOHUTex_2-oJUopAWEpAQqNwsr3c2cfCug-1tmv_DD8VpoggOqCfL8z38jWeS5DXBI4J5PoautusxoaNiRKvySXJOCorTDLPyaXKOM0rSglT8efLC-zXGuKwYOUvOOC54xqvz5PcV6uzgAaldsB0spbF32g0eLc1O2Y2zAXR_iZabvLhErXWdRxIp220M3KOtDqtYNA00SBnpPbpBi-tJdDSgBgMe6R6FFaDpV7RdabVC2qPeBuQgSN1HVT2EfS042fuNdSGWgn2QKDAG-cG1UsHL5FkrjYdXx_Mi-f5h-m1ync6_zG4mV_NUlYzkKeSMF7TO8kwqpUABwaosaaF4KzEDXDeUNzxei7KVWdtQyUmlmKpZnVcKeH6RvDv4boa6g0ZBH4MZsXG6k24nrNTiX6TXK7G0d4IQyinD0eDt0cDZXwP4IDrt953IHuIvC1ZVBcNl_iiRxDnxilaRODoQlbPeO2hPaQgW-wUQDwsgCBWFiAsQBW_-7uFEP0484uyAb7WB3SNuYrp4_5GVHGO6zzw-KH0U9UtwYm0H18eB_D9LelBoH-D-9JZ0PwVlOSvF7eeZ-MHmt5_IbCEW-R_mDOF1</recordid><startdate>19970217</startdate><enddate>19970217</enddate><creator>Kleijnen, Maurits F.</creator><creator>Huppa, Johannes B.</creator><creator>Lucin, Pero</creator><creator>Mukherjee, Siddhartha</creator><creator>Farrell, Helen</creator><creator>Campbell, Ann E.</creator><creator>Koszinowski, Ulrich H.</creator><creator>Hill, Ann B.</creator><creator>Ploegh, Hidde L.</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970217</creationdate><title>A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface</title><author>Kleijnen, Maurits F. ; Huppa, Johannes B. ; Lucin, Pero ; Mukherjee, Siddhartha ; Farrell, Helen ; Campbell, Ann E. ; Koszinowski, Ulrich H. ; Hill, Ann B. ; Ploegh, Hidde L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5713-e37946b232acccece10c5564c9fa07e0bd69d94c945fa2fd6a918c7cb7b38ce93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Viral</topic><topic>antigen presentation</topic><topic>Brefeldin A</topic><topic>Carrier Proteins - biosynthesis</topic><topic>Carrier Proteins - chemistry</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>Cyclopentanes - pharmacology</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Endoplasmic Reticulum - virology</topic><topic>ER retention</topic><topic>Fibroblasts - virology</topic><topic>Genes, Viral - genetics</topic><topic>Glycoproteins - biosynthesis</topic><topic>Glycoproteins - chemistry</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - metabolism</topic><topic>gp34</topic><topic>Hexosaminidases</topic><topic>Histocompatibility Antigens Class I - chemistry</topic><topic>Histocompatibility Antigens Class I - metabolism</topic><topic>Membrane Glycoproteins - biosynthesis</topic><topic>Membrane Glycoproteins - chemistry</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>MHC class I</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>murine cytomegalovirus</topic><topic>Muromegalovirus - genetics</topic><topic>Muromegalovirus - immunology</topic><topic>Protein Binding</topic><topic>Protein Folding</topic><topic>Protein Synthesis Inhibitors - pharmacology</topic><topic>Sequence Deletion</topic><topic>Viral Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kleijnen, Maurits F.</creatorcontrib><creatorcontrib>Huppa, Johannes B.</creatorcontrib><creatorcontrib>Lucin, Pero</creatorcontrib><creatorcontrib>Mukherjee, Siddhartha</creatorcontrib><creatorcontrib>Farrell, Helen</creatorcontrib><creatorcontrib>Campbell, Ann E.</creatorcontrib><creatorcontrib>Koszinowski, Ulrich H.</creatorcontrib><creatorcontrib>Hill, Ann B.</creatorcontrib><creatorcontrib>Ploegh, Hidde L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kleijnen, Maurits F.</au><au>Huppa, Johannes B.</au><au>Lucin, Pero</au><au>Mukherjee, Siddhartha</au><au>Farrell, Helen</au><au>Campbell, Ann E.</au><au>Koszinowski, Ulrich H.</au><au>Hill, Ann B.</au><au>Ploegh, Hidde L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface</atitle><jtitle>The EMBO journal</jtitle><stitle>EMBO J</stitle><addtitle>EMBO J</addtitle><date>1997-02-17</date><risdate>1997</risdate><volume>16</volume><issue>4</issue><spage>685</spage><epage>694</epage><pages>685-694</pages><issn>0261-4189</issn><issn>1460-2075</issn><eissn>1460-2075</eissn><abstract>Murine cytomegalovirus (MCMV) interferes with antigen presentation by means of retaining major histocompatibility complex (MHC) class I molecules in the endoplasmic reticulum (ER). Here we identify and characterize an MCMV‐encoded glycoprotein, gp34, which tightly associates with properly conformed MHC class I molecules in the ER. Gp34 is synthesized in large quantities during MCMV infection and it leaves the ER only in association with MHC class I complexes. Many but not all class I molecules are retained in the ER during the early phase of MCMV infection, and we observe an inverse correlation between amounts of gp34 synthesized during the course of infection and class I retention. An MCMV deletion mutant lacking several genes, including the gene encoding gp34, shows increased class I retention. Thus, MCMV gp34 may counteract class I retention, perhaps to decrease susceptibility of infected cells to recognition by natural killer cells.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>9049298</pmid><doi>10.1093/emboj/16.4.685</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0261-4189 |
| ispartof | The EMBO journal, 1997-02, Vol.16 (4), p.685-694 |
| issn | 0261-4189 1460-2075 1460-2075 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Animals Antibodies, Viral antigen presentation Brefeldin A Carrier Proteins - biosynthesis Carrier Proteins - chemistry Carrier Proteins - genetics Carrier Proteins - metabolism Cells, Cultured Cloning, Molecular Cyclopentanes - pharmacology Endoplasmic Reticulum - metabolism Endoplasmic Reticulum - virology ER retention Fibroblasts - virology Genes, Viral - genetics Glycoproteins - biosynthesis Glycoproteins - chemistry Glycoproteins - genetics Glycoproteins - metabolism gp34 Hexosaminidases Histocompatibility Antigens Class I - chemistry Histocompatibility Antigens Class I - metabolism Membrane Glycoproteins - biosynthesis Membrane Glycoproteins - chemistry Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism MHC class I Mice Molecular Sequence Data Molecular Weight murine cytomegalovirus Muromegalovirus - genetics Muromegalovirus - immunology Protein Binding Protein Folding Protein Synthesis Inhibitors - pharmacology Sequence Deletion Viral Proteins |
| title | A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface |
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