Enzyme synthesis in the regulation of hepatic "malic" enzyme activity
A homogeneous preparation of ;malic' enzyme (EC 1.1.1.40) from livers of thyroxine-treated rats was used to prepare in rabbits an antiserum to the enzyme that reacts monospecifically with the ;malic' enzyme in livers of rats in several physiological states. Changes in enzyme activity resul...
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| Veröffentlicht in: | Biochemical journal 1974-10, Vol.144 (1), p.149-160 |
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| creator | Murphy, G Walker, D G |
| description | A homogeneous preparation of ;malic' enzyme (EC 1.1.1.40) from livers of thyroxine-treated rats was used to prepare in rabbits an antiserum to the enzyme that reacts monospecifically with the ;malic' enzyme in livers of rats in several physiological states. Changes in enzyme activity resulting from modification of the state of the animal are hence due to an altered amount of enzyme protein. The antiserum has been used to precipitate out ;malic' enzyme from heat-treated supernatant preparations of livers from both adult and neonatal rats, in a number of physiological conditions, that had been injected 30min earlier with l-[4,5-(3)H]leucine. The low incorporations of radioactivity into the immunoprecipitable enzyme have permitted the qualitative conclusion that changed enzyme activity in adult rats arises mainly from alterations in the rate of enzyme synthesis. The marked increase in ;malic' enzyme activity that occurs naturally or as a result of thyroxine treatment of the weanling rat is likewise due to a marked increase in the rate of enzyme synthesis possibly associated with a concurrent diminished rate of enzyme degradation. |
| doi_str_mv | 10.1042/bj1440149 |
| format | Article |
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Changes in enzyme activity resulting from modification of the state of the animal are hence due to an altered amount of enzyme protein. The antiserum has been used to precipitate out ;malic' enzyme from heat-treated supernatant preparations of livers from both adult and neonatal rats, in a number of physiological conditions, that had been injected 30min earlier with l-[4,5-(3)H]leucine. The low incorporations of radioactivity into the immunoprecipitable enzyme have permitted the qualitative conclusion that changed enzyme activity in adult rats arises mainly from alterations in the rate of enzyme synthesis. The marked increase in ;malic' enzyme activity that occurs naturally or as a result of thyroxine treatment of the weanling rat is likewise due to a marked increase in the rate of enzyme synthesis possibly associated with a concurrent diminished rate of enzyme degradation.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj1440149</identifier><identifier>PMID: 4462568</identifier><language>eng</language><publisher>England</publisher><subject>Age Factors ; Animals ; Cellular Interactions and Control Processes ; Chemical Precipitation ; Female ; Immune Sera ; Immunoassay ; Leucine - administration & dosage ; Leucine - metabolism ; Liver - analysis ; Liver - enzymology ; Malate Dehydrogenase - biosynthesis ; Malate Dehydrogenase - isolation & purification ; Malate Dehydrogenase - metabolism ; Rabbits - immunology ; Rats ; Thyroxine - pharmacology ; Tritium ; Weaning</subject><ispartof>Biochemical journal, 1974-10, Vol.144 (1), p.149-160</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-71815966ed3be1a1dd9706c36d3ef8e2bc02a35c3bbdacc9ffa6f436651e87c03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1168475/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1168475/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4462568$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murphy, G</creatorcontrib><creatorcontrib>Walker, D G</creatorcontrib><title>Enzyme synthesis in the regulation of hepatic "malic" enzyme activity</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>A homogeneous preparation of ;malic' enzyme (EC 1.1.1.40) from livers of thyroxine-treated rats was used to prepare in rabbits an antiserum to the enzyme that reacts monospecifically with the ;malic' enzyme in livers of rats in several physiological states. Changes in enzyme activity resulting from modification of the state of the animal are hence due to an altered amount of enzyme protein. The antiserum has been used to precipitate out ;malic' enzyme from heat-treated supernatant preparations of livers from both adult and neonatal rats, in a number of physiological conditions, that had been injected 30min earlier with l-[4,5-(3)H]leucine. The low incorporations of radioactivity into the immunoprecipitable enzyme have permitted the qualitative conclusion that changed enzyme activity in adult rats arises mainly from alterations in the rate of enzyme synthesis. The marked increase in ;malic' enzyme activity that occurs naturally or as a result of thyroxine treatment of the weanling rat is likewise due to a marked increase in the rate of enzyme synthesis possibly associated with a concurrent diminished rate of enzyme degradation.</description><subject>Age Factors</subject><subject>Animals</subject><subject>Cellular Interactions and Control Processes</subject><subject>Chemical Precipitation</subject><subject>Female</subject><subject>Immune Sera</subject><subject>Immunoassay</subject><subject>Leucine - administration & dosage</subject><subject>Leucine - metabolism</subject><subject>Liver - analysis</subject><subject>Liver - enzymology</subject><subject>Malate Dehydrogenase - biosynthesis</subject><subject>Malate Dehydrogenase - isolation & purification</subject><subject>Malate Dehydrogenase - metabolism</subject><subject>Rabbits - immunology</subject><subject>Rats</subject><subject>Thyroxine - pharmacology</subject><subject>Tritium</subject><subject>Weaning</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtLw0AUhQdRaq0u_AHC0IXgIjqvzEw2gpT6gIIbXQ-TyU07JY-aSQrx1xtJKbq6B-53zz0chK4puadEsId0S4UgVCQnaEqFIpFWTJ-iKWFSRJIweo4uQtiSASGCTNBECMliqadouay--xJw6Kt2A8EH7Cs8KNzAuits6-sK1znewG7QDs9LW3g3xzBeWdf6vW_7S3SW2yLA1WHO0Ofz8mPxGq3eX94WT6vIcUXaSFFN40RKyHgK1NIsSxSRjsuMQ66BpY4wy2PH0zSzziV5bmUuuJQxBa0c4TP0OPruurSEzEHVNrYwu8aXtulNbb35v6n8xqzrvaFUaqHiweD2YNDUXx2E1pQ-OCgKW0HdBaOZ1FpSNYB3I-iaOoQG8uMTSsxv5-bY-cDe_E11JA8l8x-53H3V</recordid><startdate>19741001</startdate><enddate>19741001</enddate><creator>Murphy, G</creator><creator>Walker, D G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19741001</creationdate><title>Enzyme synthesis in the regulation of hepatic "malic" enzyme activity</title><author>Murphy, G ; Walker, D G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-71815966ed3be1a1dd9706c36d3ef8e2bc02a35c3bbdacc9ffa6f436651e87c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Age Factors</topic><topic>Animals</topic><topic>Cellular Interactions and Control Processes</topic><topic>Chemical Precipitation</topic><topic>Female</topic><topic>Immune Sera</topic><topic>Immunoassay</topic><topic>Leucine - administration & dosage</topic><topic>Leucine - metabolism</topic><topic>Liver - analysis</topic><topic>Liver - enzymology</topic><topic>Malate Dehydrogenase - biosynthesis</topic><topic>Malate Dehydrogenase - isolation & purification</topic><topic>Malate Dehydrogenase - metabolism</topic><topic>Rabbits - immunology</topic><topic>Rats</topic><topic>Thyroxine - pharmacology</topic><topic>Tritium</topic><topic>Weaning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murphy, G</creatorcontrib><creatorcontrib>Walker, D G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murphy, G</au><au>Walker, D G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme synthesis in the regulation of hepatic "malic" enzyme activity</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1974-10-01</date><risdate>1974</risdate><volume>144</volume><issue>1</issue><spage>149</spage><epage>160</epage><pages>149-160</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>A homogeneous preparation of ;malic' enzyme (EC 1.1.1.40) from livers of thyroxine-treated rats was used to prepare in rabbits an antiserum to the enzyme that reacts monospecifically with the ;malic' enzyme in livers of rats in several physiological states. Changes in enzyme activity resulting from modification of the state of the animal are hence due to an altered amount of enzyme protein. The antiserum has been used to precipitate out ;malic' enzyme from heat-treated supernatant preparations of livers from both adult and neonatal rats, in a number of physiological conditions, that had been injected 30min earlier with l-[4,5-(3)H]leucine. The low incorporations of radioactivity into the immunoprecipitable enzyme have permitted the qualitative conclusion that changed enzyme activity in adult rats arises mainly from alterations in the rate of enzyme synthesis. The marked increase in ;malic' enzyme activity that occurs naturally or as a result of thyroxine treatment of the weanling rat is likewise due to a marked increase in the rate of enzyme synthesis possibly associated with a concurrent diminished rate of enzyme degradation.</abstract><cop>England</cop><pmid>4462568</pmid><doi>10.1042/bj1440149</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0264-6021 |
| ispartof | Biochemical journal, 1974-10, Vol.144 (1), p.149-160 |
| issn | 0264-6021 1470-8728 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1168475 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Age Factors Animals Cellular Interactions and Control Processes Chemical Precipitation Female Immune Sera Immunoassay Leucine - administration & dosage Leucine - metabolism Liver - analysis Liver - enzymology Malate Dehydrogenase - biosynthesis Malate Dehydrogenase - isolation & purification Malate Dehydrogenase - metabolism Rabbits - immunology Rats Thyroxine - pharmacology Tritium Weaning |
| title | Enzyme synthesis in the regulation of hepatic "malic" enzyme activity |
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