Cloning of the human cDNA for the U1 RNA‐associated 70K protein

Anti‐RNP sera were used to isolate a cDNA clone for the largest polypeptide of the U1 snRNP, a protein of mol. wt 70 kd designated 70K, from a human liver cDNA library constructed in the expression vector pEX1. The cro‐beta‐galactosidase‐70K fusion protein reacted with various anti‐RNP patient sera,...

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Veröffentlicht in:	The EMBO journal 1986-12, Vol.5 (12), p.3209-3217
Hauptverfasser:	Theissen, H., Etzerodt, M., Reuter, R., Schneider, C., Lottspeich, F., Argos, P., Lührmann, R., Philipson, L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antibodies, Monoclonal Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular DNA - metabolism DNA Restriction Enzymes Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Humans Immune Sera Liver - metabolism Methods. Procedures. Technologies Molecular cloning Molecular Weight Nucleic Acid Hybridization Ribonucleoproteins - genetics Ribonucleoproteins, Small Nuclear RNA, Small Nuclear - genetics
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