T7 RNA polymerase directed expression of the Escherichia coli rrnB operon

Plasmids having Escherichia coli ribosomal DNA sequences under control of a promoter for T7 RNA polymerase have been constructed. Transcription of the rDNA sequences is dependent on T7 RNA polymerase because the tandem promoters for E. coli RNA polymerase, normally used to direct transcription of th...

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Veröffentlicht in:	The EMBO journal 1986-05, Vol.5 (5), p.1099-1103
Hauptverfasser:	Steen, R., Dahlberg, A.E., Lade, B.N., Studier, F.W., Dunn, J.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences DNA Restriction Enzymes DNA, Recombinant - metabolism DNA, Ribosomal - genetics DNA-Directed RNA Polymerases - metabolism Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Kinetics Molecular and cellular biology Molecular genetics Operon Plasmids Promoter Regions, Genetic RNA, Ribosomal - biosynthesis T-Phages - enzymology Transcription. Transcription factor. Splicing. Rna processing
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