A negative transcriptional control element located upstream of the murine c‐myc gene
We have investigated the nature of regulatory sequences within the vicinity of the murine c‐myc locus by analyzing the expression of myc‐chloramphenicol acetyl transferase (CAT) vectors transfected into a human lymphoblastoid cell line (BJAB) and a monkey fibroblast line (COS). CAT enzymatic assays...
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| Veröffentlicht in: | The EMBO journal 1986-05, Vol.5 (5), p.899-904 |
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| creator | Remmers, E.F. Yang, J.Q. Marcu, K.B. |
| description | We have investigated the nature of regulatory sequences within the vicinity of the murine c‐myc locus by analyzing the expression of myc‐chloramphenicol acetyl transferase (CAT) vectors transfected into a human lymphoblastoid cell line (BJAB) and a monkey fibroblast line (COS). CAT enzymatic assays and S1 nuclease protection experiments reveal that a negative element resides 428‐1188 bp 5′ of the first c‐myc promoter, P1. This 760‐bp segment of 5′‐flanking c‐myc DNA dramatically inhibits CAT gene expression in the pSV2CAT vector when placed in either orientation approximately 1.7 kb 3′ (and approximately 3.2 kb 5′ on the circular plasmid) from the SV40 promoter region. By employing this strategy, we were unable to identify an analogous DNA segment that is closer to or within the first c‐myc exon. We propose that this 5′ c‐myc region be termed a ‘dehancer’ since this negative element has the opposite properties of a transcriptional enhancer. |
| doi_str_mv | 10.1002/j.1460-2075.1986.tb04301.x |
| format | Article |
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CAT enzymatic assays and S1 nuclease protection experiments reveal that a negative element resides 428‐1188 bp 5′ of the first c‐myc promoter, P1. This 760‐bp segment of 5′‐flanking c‐myc DNA dramatically inhibits CAT gene expression in the pSV2CAT vector when placed in either orientation approximately 1.7 kb 3′ (and approximately 3.2 kb 5′ on the circular plasmid) from the SV40 promoter region. By employing this strategy, we were unable to identify an analogous DNA segment that is closer to or within the first c‐myc exon. We propose that this 5′ c‐myc region be termed a ‘dehancer’ since this negative element has the opposite properties of a transcriptional enhancer.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1986.tb04301.x</identifier><identifier>PMID: 3013622</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>London: Nature Publishing Group</publisher><subject>Animals ; Biological and medical sciences ; Cell Line ; Cloning, Molecular ; DNA Restriction Enzymes ; Embryo, Mammalian ; Enhancer Elements, Genetic ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genes, Regulator ; Mice ; Mice, Inbred BALB C ; Molecular and cellular biology ; Molecular genetics ; Oncogenes ; Plasmids ; Promoter Regions, Genetic ; Transcription, Genetic</subject><ispartof>The EMBO journal, 1986-05, Vol.5 (5), p.899-904</ispartof><rights>1986 European Molecular Biology Organization</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5391-b0aead2b24715661c55148ad8ae0c90c8d09d2ec91d8d811f2b8b06be4039ff63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1166880/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1166880/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8720921$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3013622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Remmers, E.F.</creatorcontrib><creatorcontrib>Yang, J.Q.</creatorcontrib><creatorcontrib>Marcu, K.B.</creatorcontrib><title>A negative transcriptional control element located upstream of the murine c‐myc gene</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>We have investigated the nature of regulatory sequences within the vicinity of the murine c‐myc locus by analyzing the expression of myc‐chloramphenicol acetyl transferase (CAT) vectors transfected into a human lymphoblastoid cell line (BJAB) and a monkey fibroblast line (COS). CAT enzymatic assays and S1 nuclease protection experiments reveal that a negative element resides 428‐1188 bp 5′ of the first c‐myc promoter, P1. This 760‐bp segment of 5′‐flanking c‐myc DNA dramatically inhibits CAT gene expression in the pSV2CAT vector when placed in either orientation approximately 1.7 kb 3′ (and approximately 3.2 kb 5′ on the circular plasmid) from the SV40 promoter region. By employing this strategy, we were unable to identify an analogous DNA segment that is closer to or within the first c‐myc exon. We propose that this 5′ c‐myc region be termed a ‘dehancer’ since this negative element has the opposite properties of a transcriptional enhancer.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>DNA Restriction Enzymes</subject><subject>Embryo, Mammalian</subject><subject>Enhancer Elements, Genetic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Genes, Regulator</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Oncogenes</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic</subject><subject>Transcription, Genetic</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkd2K1DAYhoso67h6CUIQ8az1S9qmiQfCuKx_rHiinoY0_TqbIW3GJLPunHkJXqNXYuuUQY_EowTeJy9veLLsCYWCArDn24JWHHIGTV1QKXiRWqhKoMXtnWx1iu5mK2Cc5hUV8n72IMYtANSioWfZ2QSXnLFV9mVNRtzoZG-QpKDHaILdJetH7YjxYwreEXQ44JiI80Yn7Mh-F1NAPRDfk3SNZNgHOyIxP7__GA6GbHDEh9m9XruIj5bzPPv8-vLTxdv86uObdxfrq9zUpaR5Cxp1x1pWNbTmnJq6ppXQndAIRoIRHciOoZG0E52gtGetaIG3WEEp-56X59nLY-9u3w7YmWlm0E7tgh10OCivrfo7Ge212vgbRSnnQsBU8GwpCP7rHmNSg40GndMj-n1UDZdQ0rr6JzjtrmRTzo0vjqAJPsaA_WkNBTXrU1s1O1KzIzXrU4s-dTs9fvznf05PF19T_nTJdTTa9ZMxY-MJEw0DyeiErY_YN-vw8B8D1OWHV-9_38tfXhO6jg</recordid><startdate>198605</startdate><enddate>198605</enddate><creator>Remmers, E.F.</creator><creator>Yang, J.Q.</creator><creator>Marcu, K.B.</creator><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>198605</creationdate><title>A negative transcriptional control element located upstream of the murine c‐myc gene</title><author>Remmers, E.F. ; Yang, J.Q. ; Marcu, K.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5391-b0aead2b24715661c55148ad8ae0c90c8d09d2ec91d8d811f2b8b06be4039ff63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>DNA Restriction Enzymes</topic><topic>Embryo, Mammalian</topic><topic>Enhancer Elements, Genetic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Genes, Regulator</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Oncogenes</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Remmers, E.F.</creatorcontrib><creatorcontrib>Yang, J.Q.</creatorcontrib><creatorcontrib>Marcu, K.B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Remmers, E.F.</au><au>Yang, J.Q.</au><au>Marcu, K.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A negative transcriptional control element located upstream of the murine c‐myc gene</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1986-05</date><risdate>1986</risdate><volume>5</volume><issue>5</issue><spage>899</spage><epage>904</epage><pages>899-904</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>We have investigated the nature of regulatory sequences within the vicinity of the murine c‐myc locus by analyzing the expression of myc‐chloramphenicol acetyl transferase (CAT) vectors transfected into a human lymphoblastoid cell line (BJAB) and a monkey fibroblast line (COS). CAT enzymatic assays and S1 nuclease protection experiments reveal that a negative element resides 428‐1188 bp 5′ of the first c‐myc promoter, P1. This 760‐bp segment of 5′‐flanking c‐myc DNA dramatically inhibits CAT gene expression in the pSV2CAT vector when placed in either orientation approximately 1.7 kb 3′ (and approximately 3.2 kb 5′ on the circular plasmid) from the SV40 promoter region. By employing this strategy, we were unable to identify an analogous DNA segment that is closer to or within the first c‐myc exon. We propose that this 5′ c‐myc region be termed a ‘dehancer’ since this negative element has the opposite properties of a transcriptional enhancer.</abstract><cop>London</cop><pub>Nature Publishing Group</pub><pmid>3013622</pmid><doi>10.1002/j.1460-2075.1986.tb04301.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Biological and medical sciences Cell Line Cloning, Molecular DNA Restriction Enzymes Embryo, Mammalian Enhancer Elements, Genetic Fundamental and applied biological sciences. Psychology Gene expression Genes, Regulator Mice Mice, Inbred BALB C Molecular and cellular biology Molecular genetics Oncogenes Plasmids Promoter Regions, Genetic Transcription, Genetic |
| title | A negative transcriptional control element located upstream of the murine c‐myc gene |
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