Molecular characterization of synaptophysin, a major calcium‐binding protein of the synaptic vesicle membrane

Synaptophysin, a mol. wt 38 000 glycopolypeptide of the synaptic vesicle membrane, was solubilized using Triton X‐100 and purified by immunoaffinity or ion‐exchange chromatography. From gel permeation and sucrose‐density centrifugation in H2O/D2O, a Stokes radius of 7.3 nm, a partial specific volume...

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