The potato sugar transporter SWEET1g affects apoplasmic sugar ratio and phloem-mobile tuber- and flower-inducing signals
The main phloem loader in potato, sucrose transporter StSUT1, is coexpressed with 2 members of the SWEET gene family: StSWEET11b, a clade III member of SWEET carriers assumed to be involved in sucrose efflux, and StSWEET1g, a clade I member involved in glucose efflux into the apoplast, that physical...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2024-12, Vol.197 (1) |
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| creator | Lauschke, Angelique Maibaum, Leonie Engel, Mira Eisengräber, Luise Bayer, Sina Hackel, Aleksandra Kühn, Christina |
| description | The main phloem loader in potato, sucrose transporter StSUT1, is coexpressed with 2 members of the SWEET gene family: StSWEET11b, a clade III member of SWEET carriers assumed to be involved in sucrose efflux, and StSWEET1g, a clade I member involved in glucose efflux into the apoplast, that physically interacts with StSUT1. We investigated the functionality of SWEET carriers via uptake experiments with fluorescent glucose or sucrose analogs. Inhibition or overexpression of StSWEET1g/SlSWEET1e affected tuberization and flowering in transgenic potato plants. Isolation of the apoplasmic fluid by vacuum infiltration centrifugation revealed changes in the apoplasmic hexose composition and mono-to-disaccharide ratio, affecting sink strength. Downregulation of StSWEET1g expression affected the expression of SP6A, a tuberigen, and miR172 under long-day conditions, leading to early flowering and tuberization. A systematic screen for StSWEET1g-interacting protein partners revealed several proteins affecting cell wall integrity and strengthening. StSWEET1g and the main interaction partners were strongly downregulated during tuber development. We discuss whether StSWEET1g activity might be linked to cell wall remodeling during tuber development and the switch from apoplasmic to symplasmic phloem unloading. |
| doi_str_mv | 10.1093/plphys/kiae602 |
| format | Article |
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We investigated the functionality of SWEET carriers via uptake experiments with fluorescent glucose or sucrose analogs. Inhibition or overexpression of StSWEET1g/SlSWEET1e affected tuberization and flowering in transgenic potato plants. Isolation of the apoplasmic fluid by vacuum infiltration centrifugation revealed changes in the apoplasmic hexose composition and mono-to-disaccharide ratio, affecting sink strength. Downregulation of StSWEET1g expression affected the expression of SP6A, a tuberigen, and miR172 under long-day conditions, leading to early flowering and tuberization. A systematic screen for StSWEET1g-interacting protein partners revealed several proteins affecting cell wall integrity and strengthening. StSWEET1g and the main interaction partners were strongly downregulated during tuber development. We discuss whether StSWEET1g activity might be linked to cell wall remodeling during tuber development and the switch from apoplasmic to symplasmic phloem unloading.</description><identifier>ISSN: 0032-0889</identifier><identifier>ISSN: 1532-2548</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1093/plphys/kiae602</identifier><identifier>PMID: 39509335</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Cell Wall - metabolism ; Flowers - genetics ; Flowers - growth & development ; Flowers - metabolism ; Gene Expression Regulation, Plant ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Phloem - genetics ; Phloem - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Tubers - genetics ; Plant Tubers - growth & development ; Plant Tubers - metabolism ; Plants, Genetically Modified ; Signal Transduction ; Solanum tuberosum - genetics ; Solanum tuberosum - growth & development ; Solanum tuberosum - metabolism ; Sucrose - metabolism ; Sugars - metabolism</subject><ispartof>Plant physiology (Bethesda), 2024-12, Vol.197 (1)</ispartof><rights>The Author(s) 2024. 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We investigated the functionality of SWEET carriers via uptake experiments with fluorescent glucose or sucrose analogs. Inhibition or overexpression of StSWEET1g/SlSWEET1e affected tuberization and flowering in transgenic potato plants. Isolation of the apoplasmic fluid by vacuum infiltration centrifugation revealed changes in the apoplasmic hexose composition and mono-to-disaccharide ratio, affecting sink strength. Downregulation of StSWEET1g expression affected the expression of SP6A, a tuberigen, and miR172 under long-day conditions, leading to early flowering and tuberization. A systematic screen for StSWEET1g-interacting protein partners revealed several proteins affecting cell wall integrity and strengthening. StSWEET1g and the main interaction partners were strongly downregulated during tuber development. We discuss whether StSWEET1g activity might be linked to cell wall remodeling during tuber development and the switch from apoplasmic to symplasmic phloem unloading.</description><subject>Cell Wall - metabolism</subject><subject>Flowers - genetics</subject><subject>Flowers - growth & development</subject><subject>Flowers - metabolism</subject><subject>Gene Expression Regulation, Plant</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Phloem - genetics</subject><subject>Phloem - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Tubers - genetics</subject><subject>Plant Tubers - growth & development</subject><subject>Plant Tubers - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Signal Transduction</subject><subject>Solanum tuberosum - genetics</subject><subject>Solanum tuberosum - growth & development</subject><subject>Solanum tuberosum - metabolism</subject><subject>Sucrose - metabolism</subject><subject>Sugars - metabolism</subject><issn>0032-0889</issn><issn>1532-2548</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUTuP1DAQthCIWw5aSuSSJnd-JE5cIXRaHtJJFCyitMaOnTU4sbEd4P49gV1OUM0nfY-Z0YfQc0quKJH8OoV0vCvXXz1YQdgDtKMdZw3r2uEh2hGyYTIM8gI9KeULIYRy2j5GF1x2m5l3O_TzcLQ4xQo14rJOkHHNsJQUc7UZf_y83x_ohME5a2rBkGIKUGZvzuIM1UcMy4jTMUQ7N3PUPlhcV21z84dwIf7YsF_G1fhlwsVPC4TyFD1y27DPzvMSfXqzP9y8a24_vH1_8_q2MYyL2oheMsusJEwPwsnWcamlMD0fyGg16YF2BjR0ko_tqBlxILXpCBAnO9Ebxy_Rq1NuWvVsR2OX7cGgUvYz5DsVwav_mcUf1RS_K0qF4D3pt4SX54Qcv622VDX7YmwIsNi4FsUpGzgdSCs26dVJanIsJVt3v4cS9bsvdepLnfvaDC_-ve5e_rcg_gsSXZdT</recordid><startdate>20241223</startdate><enddate>20241223</enddate><creator>Lauschke, Angelique</creator><creator>Maibaum, Leonie</creator><creator>Engel, Mira</creator><creator>Eisengräber, Luise</creator><creator>Bayer, Sina</creator><creator>Hackel, Aleksandra</creator><creator>Kühn, Christina</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8370-9706</orcidid></search><sort><creationdate>20241223</creationdate><title>The potato sugar transporter SWEET1g affects apoplasmic sugar ratio and phloem-mobile tuber- and flower-inducing signals</title><author>Lauschke, Angelique ; 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We investigated the functionality of SWEET carriers via uptake experiments with fluorescent glucose or sucrose analogs. Inhibition or overexpression of StSWEET1g/SlSWEET1e affected tuberization and flowering in transgenic potato plants. Isolation of the apoplasmic fluid by vacuum infiltration centrifugation revealed changes in the apoplasmic hexose composition and mono-to-disaccharide ratio, affecting sink strength. Downregulation of StSWEET1g expression affected the expression of SP6A, a tuberigen, and miR172 under long-day conditions, leading to early flowering and tuberization. A systematic screen for StSWEET1g-interacting protein partners revealed several proteins affecting cell wall integrity and strengthening. StSWEET1g and the main interaction partners were strongly downregulated during tuber development. 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| subjects | Cell Wall - metabolism Flowers - genetics Flowers - growth & development Flowers - metabolism Gene Expression Regulation, Plant Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Phloem - genetics Phloem - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plant Tubers - genetics Plant Tubers - growth & development Plant Tubers - metabolism Plants, Genetically Modified Signal Transduction Solanum tuberosum - genetics Solanum tuberosum - growth & development Solanum tuberosum - metabolism Sucrose - metabolism Sugars - metabolism |
| title | The potato sugar transporter SWEET1g affects apoplasmic sugar ratio and phloem-mobile tuber- and flower-inducing signals |
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