An investigation by dielectric methods of hydration in myoglobin solutions
The permittivities of three solutions of sperm-whale myoglobin of different concentrations were measured in the frequency range 300-1300MHz at 20 degrees C by using a coaxial-line technique. These results were combined with those measured previously at frequencies below 10MHz. Two methods are descri...
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| Veröffentlicht in: | Biochemical journal 1974-05, Vol.139 (2), p.375-380 |
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| container_title | Biochemical journal |
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| creator | Grant, E H Mitton, B G South, G P Sheppard, R J |
| description | The permittivities of three solutions of sperm-whale myoglobin of different concentrations were measured in the frequency range 300-1300MHz at 20 degrees C by using a coaxial-line technique. These results were combined with those measured previously at frequencies below 10MHz. Two methods are described for calculating the extent of macromolecular hydration from the data. The more reliable method yields results of approx. 0.25g of H(2)O/g of protein, which is in satisfactory agreement with the theoretically calculated value. Agreement with the value found from the rotational motion of the molecule is not so close, which is probably caused by the different meanings that may be ascribed to the term hydration. |
| doi_str_mv | 10.1042/bj1390375 |
| format | Article |
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These results were combined with those measured previously at frequencies below 10MHz. Two methods are described for calculating the extent of macromolecular hydration from the data. The more reliable method yields results of approx. 0.25g of H(2)O/g of protein, which is in satisfactory agreement with the theoretically calculated value. 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These results were combined with those measured previously at frequencies below 10MHz. Two methods are described for calculating the extent of macromolecular hydration from the data. The more reliable method yields results of approx. 0.25g of H(2)O/g of protein, which is in satisfactory agreement with the theoretically calculated value. Agreement with the value found from the rotational motion of the molecule is not so close, which is probably caused by the different meanings that may be ascribed to the term hydration.</description><subject>Animals</subject><subject>Biophysical Phenomena</subject><subject>Biophysics</subject><subject>Dialysis</subject><subject>Electrochemistry - methods</subject><subject>Mathematics</subject><subject>Myoglobin - analysis</subject><subject>Proteins</subject><subject>Radio Waves</subject><subject>Solutions</subject><subject>Spectrophotometry</subject><subject>Water</subject><subject>Whales</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE9Lw0AQxRdRaq0e_ABCToKH6Gx2s5tcBCn-peBFz8tmd9JuSbI1mxTy7U1pKXqaYebHmzePkGsK9xR48lCsKcuByfSETCmXEGcyyU7JFBLBYwEJPScXIawBKAcOEzLhXKZpnkzJx1MTuWaLoXNL3TnfRMUQWYcVmq51JqqxW3kbIl9Gq8G2e8Q1UT34ZeWLsQu-6nfTcEnOSl0FvDrUGfl-ef6av8WLz9f3-dMiNkxCF2sodWFLJg3aIs0EFWmSCoFa5FCOvjgIoyUgIuU8FdJmDIQuZJYxK3iObEYe97qbvqjRGmy6Vldq07pat4Py2qn_m8at1NJvFaVCJDkbBW4PAq3_6cfXVe2CwarSDfo-qGxMjQHLR_BuD5rWh9BieTxCQe2CV8fgR_bmr6sjeUia_QLnS3-b</recordid><startdate>19740501</startdate><enddate>19740501</enddate><creator>Grant, E H</creator><creator>Mitton, B G</creator><creator>South, G P</creator><creator>Sheppard, R J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19740501</creationdate><title>An investigation by dielectric methods of hydration in myoglobin solutions</title><author>Grant, E H ; Mitton, B G ; South, G P ; Sheppard, R J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-a0fabdf37cedb5861652566ea690f447406ca70eee144567d8306ab7883d649e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Animals</topic><topic>Biophysical Phenomena</topic><topic>Biophysics</topic><topic>Dialysis</topic><topic>Electrochemistry - methods</topic><topic>Mathematics</topic><topic>Myoglobin - analysis</topic><topic>Proteins</topic><topic>Radio Waves</topic><topic>Solutions</topic><topic>Spectrophotometry</topic><topic>Water</topic><topic>Whales</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grant, E H</creatorcontrib><creatorcontrib>Mitton, B G</creatorcontrib><creatorcontrib>South, G P</creatorcontrib><creatorcontrib>Sheppard, R J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grant, E H</au><au>Mitton, B G</au><au>South, G P</au><au>Sheppard, R J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An investigation by dielectric methods of hydration in myoglobin solutions</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1974-05-01</date><risdate>1974</risdate><volume>139</volume><issue>2</issue><spage>375</spage><epage>380</epage><pages>375-380</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>The permittivities of three solutions of sperm-whale myoglobin of different concentrations were measured in the frequency range 300-1300MHz at 20 degrees C by using a coaxial-line technique. 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| ispartof | Biochemical journal, 1974-05, Vol.139 (2), p.375-380 |
| issn | 0264-6021 1470-8728 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Biophysical Phenomena Biophysics Dialysis Electrochemistry - methods Mathematics Myoglobin - analysis Proteins Radio Waves Solutions Spectrophotometry Water Whales |
| title | An investigation by dielectric methods of hydration in myoglobin solutions |
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