Evaluation of the effect of BioFire FilmArray nested multiplex polymerase chain reaction method on rapid pathogen identification and antimicrobial stewardship in sepsis
In this study, we aimed to assess the effect of the BioFire FilmArray Blood Culture Identification 2 panel on agent identification and antimicrobial stewardship in patients with a critical state of sepsis secondary to bloodstream infection. This study was designed as a prospective observational stud...
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| Veröffentlicht in: | Revista da Associacao Medica Brasileira (1992) 2024-01, Vol.70 (12), p.e20241038 |
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| creator | Kış, Tuba Tatlı Biçmen, Can Yıldırım, Suleyman Ediboğlu, Ozlem Yıldız, Fatma Sebnem Gunduz, Ayriz Tuba Demirci, Ferhat Kıraklı, Cenk |
| description | In this study, we aimed to assess the effect of the BioFire FilmArray Blood Culture Identification 2 panel on agent identification and antimicrobial stewardship in patients with a critical state of sepsis secondary to bloodstream infection.
This study was designed as a prospective observational study. Patients who developed sepsis and septic shock secondary to bloodstream infection in the intensive care unit were included in the study. Concordance in both monomicrobial and polymicrobial results of Blood Culture Identification 2 panel and conventional blood culture, test result times, and antibiotherapy changes according to Blood Culture Identification 2 panel results were evaluated.
In monomicrobial samples, sensitivity and specificity were 97.1% (95%CI 84.6-99.3) and 100% (95%CI 66.3-100), respectively, for gram-negative pathogens and 85.7% (95%CI 42.1-99.6) and 100% (95%CI 90.2-100), respectively, for gram-positive pathogens. In polymicrobial samples, Blood Culture Identification 2 panel results were 79% in concordance with conventional blood culture results. In this study, when the final turnaround time of the Blood Culture Identification 2 panel was compared with culture results, the Blood Culture Identification 2 panel was on average 1 day, 5 h, and 35 min faster than the culture (p |
| doi_str_mv | 10.1590/1806-9282.20241038 |
| format | Magazinearticle |
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This study was designed as a prospective observational study. Patients who developed sepsis and septic shock secondary to bloodstream infection in the intensive care unit were included in the study. Concordance in both monomicrobial and polymicrobial results of Blood Culture Identification 2 panel and conventional blood culture, test result times, and antibiotherapy changes according to Blood Culture Identification 2 panel results were evaluated.
In monomicrobial samples, sensitivity and specificity were 97.1% (95%CI 84.6-99.3) and 100% (95%CI 66.3-100), respectively, for gram-negative pathogens and 85.7% (95%CI 42.1-99.6) and 100% (95%CI 90.2-100), respectively, for gram-positive pathogens. In polymicrobial samples, Blood Culture Identification 2 panel results were 79% in concordance with conventional blood culture results. In this study, when the final turnaround time of the Blood Culture Identification 2 panel was compared with culture results, the Blood Culture Identification 2 panel was on average 1 day, 5 h, and 35 min faster than the culture (p<0.01).
Blood Culture Identification 2 testing is a reliable tool for rapid pathogen and antimicrobial susceptibility detection in critically ill sepsis patients. The use of the Blood Culture Identification 2 panel in patients with sepsis and/or septic shock, where the transition to targeted antibiotherapy is critical, may improve patient outcomes.</description><identifier>ISSN: 0104-4230</identifier><identifier>EISSN: 1806-9282</identifier><identifier>DOI: 10.1590/1806-9282.20241038</identifier><identifier>PMID: 39699483</identifier><language>eng</language><publisher>Brazil: Associação Médica Brasileira</publisher><subject>Adult ; Aged ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - therapeutic use ; Antimicrobial Stewardship ; Blood Culture - methods ; Female ; Gram-Negative Bacteria - drug effects ; Gram-Negative Bacteria - isolation & purification ; Gram-Positive Bacteria - drug effects ; Gram-Positive Bacteria - isolation & purification ; Humans ; Intensive Care Units ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Multiplex Polymerase Chain Reaction - methods ; Original ; Prospective Studies ; Sensitivity and Specificity ; Sepsis - drug therapy ; Sepsis - microbiology</subject><ispartof>Revista da Associacao Medica Brasileira (1992), 2024-01, Vol.70 (12), p.e20241038</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1911-a0c51cd44e4c225ca4e085dc83a4d3c82d6d20e5798ea5f75f61ce83bb70f3bf3</cites><orcidid>0000-0002-5999-3399 ; 0000-0001-5392-570X ; 0000-0001-6952-3748 ; 0000-0002-4543-7228 ; 0000-0001-6013-7330 ; 0000-0001-9856-3431 ; 0000-0001-5133-7250 ; 0000-0003-2365-9863</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656528/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656528/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,727,780,784,885,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39699483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kış, Tuba Tatlı</creatorcontrib><creatorcontrib>Biçmen, Can</creatorcontrib><creatorcontrib>Yıldırım, Suleyman</creatorcontrib><creatorcontrib>Ediboğlu, Ozlem</creatorcontrib><creatorcontrib>Yıldız, Fatma Sebnem</creatorcontrib><creatorcontrib>Gunduz, Ayriz Tuba</creatorcontrib><creatorcontrib>Demirci, Ferhat</creatorcontrib><creatorcontrib>Kıraklı, Cenk</creatorcontrib><title>Evaluation of the effect of BioFire FilmArray nested multiplex polymerase chain reaction method on rapid pathogen identification and antimicrobial stewardship in sepsis</title><title>Revista da Associacao Medica Brasileira (1992)</title><addtitle>Rev Assoc Med Bras (1992)</addtitle><description>In this study, we aimed to assess the effect of the BioFire FilmArray Blood Culture Identification 2 panel on agent identification and antimicrobial stewardship in patients with a critical state of sepsis secondary to bloodstream infection.
This study was designed as a prospective observational study. Patients who developed sepsis and septic shock secondary to bloodstream infection in the intensive care unit were included in the study. Concordance in both monomicrobial and polymicrobial results of Blood Culture Identification 2 panel and conventional blood culture, test result times, and antibiotherapy changes according to Blood Culture Identification 2 panel results were evaluated.
In monomicrobial samples, sensitivity and specificity were 97.1% (95%CI 84.6-99.3) and 100% (95%CI 66.3-100), respectively, for gram-negative pathogens and 85.7% (95%CI 42.1-99.6) and 100% (95%CI 90.2-100), respectively, for gram-positive pathogens. In polymicrobial samples, Blood Culture Identification 2 panel results were 79% in concordance with conventional blood culture results. In this study, when the final turnaround time of the Blood Culture Identification 2 panel was compared with culture results, the Blood Culture Identification 2 panel was on average 1 day, 5 h, and 35 min faster than the culture (p<0.01).
Blood Culture Identification 2 testing is a reliable tool for rapid pathogen and antimicrobial susceptibility detection in critically ill sepsis patients. The use of the Blood Culture Identification 2 panel in patients with sepsis and/or septic shock, where the transition to targeted antibiotherapy is critical, may improve patient outcomes.</description><subject>Adult</subject><subject>Aged</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Antimicrobial Stewardship</subject><subject>Blood Culture - methods</subject><subject>Female</subject><subject>Gram-Negative Bacteria - drug effects</subject><subject>Gram-Negative Bacteria - isolation & purification</subject><subject>Gram-Positive Bacteria - drug effects</subject><subject>Gram-Positive Bacteria - isolation & purification</subject><subject>Humans</subject><subject>Intensive Care Units</subject><subject>Male</subject><subject>Microbial Sensitivity Tests</subject><subject>Middle Aged</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Original</subject><subject>Prospective Studies</subject><subject>Sensitivity and Specificity</subject><subject>Sepsis - drug therapy</subject><subject>Sepsis - microbiology</subject><issn>0104-4230</issn><issn>1806-9282</issn><fulltext>true</fulltext><rsrctype>magazinearticle</rsrctype><creationdate>2024</creationdate><recordtype>magazinearticle</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1uFDEQhS0EIqOQC7BAvkAH__a4VyhEGUCKlA2sW9V2OV1S_8nuCcyNOCYehozIwipXWe97ZT3G3ktxLW0jPkon6qpRTl0roYwU2r1im_PwNdsIKUxllBYX7Cpn6oQSW2uVdG_ZhW7qpjFOb9jvuycY9rDSPPE58rVHjjGiX4_dZ5p3lJDvaBhvUoIDnzCvGPi4H1ZaBvzFl3k4jJggI_c90MQTgv9LG3Ht58DLLcFCgS9Q-kecOAWcVorkT64whXJWGsmnuSMYeLH4CSnknhZeiBmXTPkdexNhyHj1r16yH7u777dfq_uHL99ub-4rLxspKxDeSh-MQeOVsh4MCmeDdxpM0N6pUAcl0G4bh2Dj1sZaenS667Yi6i7qS_bpxF323YjBl10TDO2SaIR0aGeg9uXLRH37OD-1Uta2tsoVgjoRyn9yThjPYinaY3btMab2GFP7nF0Rffjf9ix5Tkr_Abl7mvo</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Kış, Tuba Tatlı</creator><creator>Biçmen, Can</creator><creator>Yıldırım, Suleyman</creator><creator>Ediboğlu, Ozlem</creator><creator>Yıldız, Fatma Sebnem</creator><creator>Gunduz, Ayriz Tuba</creator><creator>Demirci, Ferhat</creator><creator>Kıraklı, Cenk</creator><general>Associação Médica Brasileira</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5999-3399</orcidid><orcidid>https://orcid.org/0000-0001-5392-570X</orcidid><orcidid>https://orcid.org/0000-0001-6952-3748</orcidid><orcidid>https://orcid.org/0000-0002-4543-7228</orcidid><orcidid>https://orcid.org/0000-0001-6013-7330</orcidid><orcidid>https://orcid.org/0000-0001-9856-3431</orcidid><orcidid>https://orcid.org/0000-0001-5133-7250</orcidid><orcidid>https://orcid.org/0000-0003-2365-9863</orcidid></search><sort><creationdate>20240101</creationdate><title>Evaluation of the effect of BioFire FilmArray nested multiplex polymerase chain reaction method on rapid pathogen identification and antimicrobial stewardship in sepsis</title><author>Kış, Tuba Tatlı ; Biçmen, Can ; Yıldırım, Suleyman ; Ediboğlu, Ozlem ; Yıldız, Fatma Sebnem ; Gunduz, Ayriz Tuba ; Demirci, Ferhat ; Kıraklı, Cenk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1911-a0c51cd44e4c225ca4e085dc83a4d3c82d6d20e5798ea5f75f61ce83bb70f3bf3</frbrgroupid><rsrctype>magazinearticle</rsrctype><prefilter>magazinearticle</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Antimicrobial Stewardship</topic><topic>Blood Culture - methods</topic><topic>Female</topic><topic>Gram-Negative Bacteria - drug effects</topic><topic>Gram-Negative Bacteria - isolation & purification</topic><topic>Gram-Positive Bacteria - drug effects</topic><topic>Gram-Positive Bacteria - isolation & purification</topic><topic>Humans</topic><topic>Intensive Care Units</topic><topic>Male</topic><topic>Microbial Sensitivity Tests</topic><topic>Middle Aged</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Original</topic><topic>Prospective Studies</topic><topic>Sensitivity and Specificity</topic><topic>Sepsis - drug therapy</topic><topic>Sepsis - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kış, Tuba Tatlı</creatorcontrib><creatorcontrib>Biçmen, Can</creatorcontrib><creatorcontrib>Yıldırım, Suleyman</creatorcontrib><creatorcontrib>Ediboğlu, Ozlem</creatorcontrib><creatorcontrib>Yıldız, Fatma Sebnem</creatorcontrib><creatorcontrib>Gunduz, Ayriz Tuba</creatorcontrib><creatorcontrib>Demirci, Ferhat</creatorcontrib><creatorcontrib>Kıraklı, Cenk</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Revista da Associacao Medica Brasileira (1992)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kış, Tuba Tatlı</au><au>Biçmen, Can</au><au>Yıldırım, Suleyman</au><au>Ediboğlu, Ozlem</au><au>Yıldız, Fatma Sebnem</au><au>Gunduz, Ayriz Tuba</au><au>Demirci, Ferhat</au><au>Kıraklı, Cenk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the effect of BioFire FilmArray nested multiplex polymerase chain reaction method on rapid pathogen identification and antimicrobial stewardship in sepsis</atitle><jtitle>Revista da Associacao Medica Brasileira (1992)</jtitle><addtitle>Rev Assoc Med Bras (1992)</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>70</volume><issue>12</issue><spage>e20241038</spage><pages>e20241038-</pages><issn>0104-4230</issn><eissn>1806-9282</eissn><abstract>In this study, we aimed to assess the effect of the BioFire FilmArray Blood Culture Identification 2 panel on agent identification and antimicrobial stewardship in patients with a critical state of sepsis secondary to bloodstream infection.
This study was designed as a prospective observational study. Patients who developed sepsis and septic shock secondary to bloodstream infection in the intensive care unit were included in the study. Concordance in both monomicrobial and polymicrobial results of Blood Culture Identification 2 panel and conventional blood culture, test result times, and antibiotherapy changes according to Blood Culture Identification 2 panel results were evaluated.
In monomicrobial samples, sensitivity and specificity were 97.1% (95%CI 84.6-99.3) and 100% (95%CI 66.3-100), respectively, for gram-negative pathogens and 85.7% (95%CI 42.1-99.6) and 100% (95%CI 90.2-100), respectively, for gram-positive pathogens. In polymicrobial samples, Blood Culture Identification 2 panel results were 79% in concordance with conventional blood culture results. In this study, when the final turnaround time of the Blood Culture Identification 2 panel was compared with culture results, the Blood Culture Identification 2 panel was on average 1 day, 5 h, and 35 min faster than the culture (p<0.01).
Blood Culture Identification 2 testing is a reliable tool for rapid pathogen and antimicrobial susceptibility detection in critically ill sepsis patients. The use of the Blood Culture Identification 2 panel in patients with sepsis and/or septic shock, where the transition to targeted antibiotherapy is critical, may improve patient outcomes.</abstract><cop>Brazil</cop><pub>Associação Médica Brasileira</pub><pmid>39699483</pmid><doi>10.1590/1806-9282.20241038</doi><orcidid>https://orcid.org/0000-0002-5999-3399</orcidid><orcidid>https://orcid.org/0000-0001-5392-570X</orcidid><orcidid>https://orcid.org/0000-0001-6952-3748</orcidid><orcidid>https://orcid.org/0000-0002-4543-7228</orcidid><orcidid>https://orcid.org/0000-0001-6013-7330</orcidid><orcidid>https://orcid.org/0000-0001-9856-3431</orcidid><orcidid>https://orcid.org/0000-0001-5133-7250</orcidid><orcidid>https://orcid.org/0000-0003-2365-9863</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adult Aged Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Antimicrobial Stewardship Blood Culture - methods Female Gram-Negative Bacteria - drug effects Gram-Negative Bacteria - isolation & purification Gram-Positive Bacteria - drug effects Gram-Positive Bacteria - isolation & purification Humans Intensive Care Units Male Microbial Sensitivity Tests Middle Aged Multiplex Polymerase Chain Reaction - methods Original Prospective Studies Sensitivity and Specificity Sepsis - drug therapy Sepsis - microbiology |
| title | Evaluation of the effect of BioFire FilmArray nested multiplex polymerase chain reaction method on rapid pathogen identification and antimicrobial stewardship in sepsis |
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