Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix
The orientation and function of smooth muscle in the cervix may contribute to the important biomechanical properties that change during pregnancy. Thus, this study examined the three-dimensional structure, smooth muscle phenotype, and mechanical and contractile functions of the upper and lower cervi...
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| Veröffentlicht in: | Science advances 2024-12, Vol.10 (51), p.eadr3530 |
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| creator | Hansen, Christopher J Rogers, Jackson H Brown, Alexus J Boatwright, Naoko Siricilla, Shajila O'Brien, Christine M Panja, Sourav Nichols, Cameron M Devanathan, Kanchana Hardy, Benjamin M Does, Mark D Anderson, Adam W Paria, Bibhash C Mahadevan-Jansen, Anita Reese, Jeff Herington, Jennifer L |
| description | The orientation and function of smooth muscle in the cervix may contribute to the important biomechanical properties that change during pregnancy. Thus, this study examined the three-dimensional structure, smooth muscle phenotype, and mechanical and contractile functions of the upper and lower cervix of nongravid (not pregnant) and gravid (pregnant) mice. In gravid cervix, we uncovered region-specific changes in the structure and organization of fiber tracts. We also detected a greater proportion of contractile smooth muscle cells (SMCs), but an equal proportion of synthetic SMCs, in the upper versus lower cervix. Furthermore, we revealed that the lower cervix had infrequent spontaneous contractions, distension had a minimal effect on contractility, and the upper cervix had forceful contractions in response to labor-inducing agents (oxytocin and prostaglandin E
). These findings identify regional differences in cervix contractility related to contractile SMC content and fiber organization, which could be targeted with diagnostic technologies and for therapeutic intervention. |
| doi_str_mv | 10.1126/sciadv.adr3530 |
| format | Article |
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). These findings identify regional differences in cervix contractility related to contractile SMC content and fiber organization, which could be targeted with diagnostic technologies and for therapeutic intervention.</description><subject>Animals</subject><subject>Biomedicine and Life Sciences</subject><subject>Cervix Uteri - cytology</subject><subject>Cervix Uteri - physiology</subject><subject>Engineering</subject><subject>Female</subject><subject>Mice</subject><subject>Muscle Contraction - physiology</subject><subject>Myocytes, Smooth Muscle - cytology</subject><subject>Myocytes, Smooth Muscle - metabolism</subject><subject>Myocytes, Smooth Muscle - physiology</subject><subject>Phenotype</subject><subject>Physiology</subject><subject>Pregnancy</subject><subject>SciAdv r-articles</subject><subject>Uterine Contraction - physiology</subject><issn>2375-2548</issn><issn>2375-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1v1DAQtRCIVqVXjshHDs0Sf8TZnBCqoEWqhITaszW2J7tGiR1s74rtT-ivJqssVTnN15s38_QIec_qFWNcfcrWg9uvwCXRiPoVOeeibSreyPXrF_kZucz5V13XTCrVsO4tOROd6oTk4pw8_cSNjwEG6nzfY8JgMVMfaNkmxMr5EUNeAL03mGhMGwj-EcrcvKJ5jLFs6bjLdkBqcRjotMUQy2HCKwrBURtDSWCLH3w5LMRIp4SbAKHQMe7ycS_t_Z935E0PQ8bLU7wgD9--3l_fVnc_br5ff7mrLBeqVAKsc43DplsjUwDQcsfqdg2GccNRutoII3vbC9PZhiNX0jFp58qsQRkmLsjnhXfamRGdxeODg56SHyEddASv_58Ev9WbuNeMqUaqtpsZPp4YUvy9w1z06PNRPAScBWnBZMt4JwWfoasFalPMOWH_fIfV-miiXkzUJxPnhQ8vv3uG_7NM_AUzs5_J</recordid><startdate>20241220</startdate><enddate>20241220</enddate><creator>Hansen, Christopher J</creator><creator>Rogers, Jackson H</creator><creator>Brown, Alexus J</creator><creator>Boatwright, Naoko</creator><creator>Siricilla, Shajila</creator><creator>O'Brien, Christine M</creator><creator>Panja, Sourav</creator><creator>Nichols, Cameron M</creator><creator>Devanathan, Kanchana</creator><creator>Hardy, Benjamin M</creator><creator>Does, Mark D</creator><creator>Anderson, Adam W</creator><creator>Paria, Bibhash C</creator><creator>Mahadevan-Jansen, Anita</creator><creator>Reese, Jeff</creator><creator>Herington, Jennifer L</creator><general>American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0009-0005-3138-5307</orcidid><orcidid>https://orcid.org/0000-0002-0491-3769</orcidid><orcidid>https://orcid.org/0000-0001-7084-184X</orcidid><orcidid>https://orcid.org/0000-0001-5353-9163</orcidid><orcidid>https://orcid.org/0009-0007-4589-916X</orcidid><orcidid>https://orcid.org/0000-0002-1183-8648</orcidid><orcidid>https://orcid.org/0000-0002-7105-0529</orcidid><orcidid>https://orcid.org/0000-0003-3213-775X</orcidid><orcidid>https://orcid.org/0000-0001-8751-6146</orcidid><orcidid>https://orcid.org/0009-0002-7421-4741</orcidid><orcidid>https://orcid.org/0000-0001-8540-5266</orcidid></search><sort><creationdate>20241220</creationdate><title>Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix</title><author>Hansen, Christopher J ; 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Thus, this study examined the three-dimensional structure, smooth muscle phenotype, and mechanical and contractile functions of the upper and lower cervix of nongravid (not pregnant) and gravid (pregnant) mice. In gravid cervix, we uncovered region-specific changes in the structure and organization of fiber tracts. We also detected a greater proportion of contractile smooth muscle cells (SMCs), but an equal proportion of synthetic SMCs, in the upper versus lower cervix. Furthermore, we revealed that the lower cervix had infrequent spontaneous contractions, distension had a minimal effect on contractility, and the upper cervix had forceful contractions in response to labor-inducing agents (oxytocin and prostaglandin E
). These findings identify regional differences in cervix contractility related to contractile SMC content and fiber organization, which could be targeted with diagnostic technologies and for therapeutic intervention.</abstract><cop>United States</cop><pub>American Association for the Advancement of Science</pub><pmid>39693423</pmid><doi>10.1126/sciadv.adr3530</doi><orcidid>https://orcid.org/0009-0005-3138-5307</orcidid><orcidid>https://orcid.org/0000-0002-0491-3769</orcidid><orcidid>https://orcid.org/0000-0001-7084-184X</orcidid><orcidid>https://orcid.org/0000-0001-5353-9163</orcidid><orcidid>https://orcid.org/0009-0007-4589-916X</orcidid><orcidid>https://orcid.org/0000-0002-1183-8648</orcidid><orcidid>https://orcid.org/0000-0002-7105-0529</orcidid><orcidid>https://orcid.org/0000-0003-3213-775X</orcidid><orcidid>https://orcid.org/0000-0001-8751-6146</orcidid><orcidid>https://orcid.org/0009-0002-7421-4741</orcidid><orcidid>https://orcid.org/0000-0001-8540-5266</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biomedicine and Life Sciences Cervix Uteri - cytology Cervix Uteri - physiology Engineering Female Mice Muscle Contraction - physiology Myocytes, Smooth Muscle - cytology Myocytes, Smooth Muscle - metabolism Myocytes, Smooth Muscle - physiology Phenotype Physiology Pregnancy SciAdv r-articles Uterine Contraction - physiology |
| title | Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix |
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