Defoliation Induces Fructan 1-Exohydrolase II in Witloof Chicory Roots. Cloning and Purification of Two Isoforms, Fructan 1-Exohydrolase IIa and Fructan 1-Exohydrolase IIb. Mass Fingerprint of the Fructan 1-Exohydrolase II Enzymes
The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be purified from forced chicory roots as well as from the etiolated "Belgian endive" leaves where the 1-FEH IIa...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2001-07, Vol.126 (3), p.1186-1195 |
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| creator | Van den Ende, Wim An Michiels Dominik Van Wonterghem Stefan P. Clerens Joke De Roover André J. Van Laere |
| description | The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be purified from forced chicory roots as well as from the etiolated "Belgian endive" leaves where the 1-FEH IIa isoform is present in higher concentrations. Full-length cDNAs were obtained by a combination of reverse transcriptase-polymerase chain reaction (PCR), PCR and 5′- and 3′-rapid amplification of cDNA ends using primers based on N-terminal and conserved amino acid sequences. 1-FEH IIa and 1-FEH IIb cDNA-derived amino acid sequences are most homologous to a new group of plant glycosyl hydrolases harboring cell wall-type enzymes with acid isoelectric points. Unlike the observed expression profiles of chicory 1-FEH I, northern analysis revealed that 1-FEH II is expressed when young chicory plants are defoliated, suggesting that this enzyme can be induced at any developmental stage when large energy supplies are necessary (regrowth after defoliation). |
| doi_str_mv | 10.1104/pp.126.3.1186 |
| format | Article |
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Cloning and Purification of Two Isoforms, Fructan 1-Exohydrolase IIa and Fructan 1-Exohydrolase IIb. Mass Fingerprint of the Fructan 1-Exohydrolase II Enzymes</title><source>Jstor Complete Legacy</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Van den Ende, Wim ; An Michiels ; Dominik Van Wonterghem ; Stefan P. Clerens ; Joke De Roover ; André J. Van Laere</creator><creatorcontrib>Van den Ende, Wim ; An Michiels ; Dominik Van Wonterghem ; Stefan P. Clerens ; Joke De Roover ; André J. Van Laere</creatorcontrib><description>The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be purified from forced chicory roots as well as from the etiolated "Belgian endive" leaves where the 1-FEH IIa isoform is present in higher concentrations. Full-length cDNAs were obtained by a combination of reverse transcriptase-polymerase chain reaction (PCR), PCR and 5′- and 3′-rapid amplification of cDNA ends using primers based on N-terminal and conserved amino acid sequences. 1-FEH IIa and 1-FEH IIb cDNA-derived amino acid sequences are most homologous to a new group of plant glycosyl hydrolases harboring cell wall-type enzymes with acid isoelectric points. Unlike the observed expression profiles of chicory 1-FEH I, northern analysis revealed that 1-FEH II is expressed when young chicory plants are defoliated, suggesting that this enzyme can be induced at any developmental stage when large energy supplies are necessary (regrowth after defoliation).</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.126.3.1186</identifier><identifier>PMID: 11457968</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>Agronomy. Soil science and plant productions ; Amino Acid Sequence ; Amino acids ; beta-Fructofuranosidase ; Biological and medical sciences ; Cell walls ; Cichorium intybus ; Cichorium intybus - enzymology ; Cichorium intybus - genetics ; Cloning, Molecular ; Complementary DNA ; Economic plant physiology ; Environmental Stress and Adaptation ; Enzymes ; Exons ; fructan 1-exohydrolase ; Fructans ; Fundamental and applied biological sciences. Psychology ; Glycoside Hydrolases - genetics ; Glycoside Hydrolases - isolation & purification ; Isoenzymes - genetics ; Isoenzymes - isolation & purification ; Metabolism ; Molecular Sequence Data ; Nutrition. Photosynthesis. Respiration. Metabolism ; Peptide Fragments - metabolism ; Plant Leaves - physiology ; Plant physiology and development ; Plant roots ; Plant Roots - enzymology ; Plants ; Polymerase chain reaction ; Protein isoforms ; RNA ; Sequence Homology, Amino Acid</subject><ispartof>Plant physiology (Bethesda), 2001-07, Vol.126 (3), p.1186-1195</ispartof><rights>Copyright 2001 American Society of Plant Biologists</rights><rights>2001 INIST-CNRS</rights><rights>Copyright © 2001, American Society of Plant Physiologists 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-e38ee494fe1ada247a77a643077f26a2e4009728fd2760a8c6f514cf0345598d3</citedby><cites>FETCH-LOGICAL-c566t-e38ee494fe1ada247a77a643077f26a2e4009728fd2760a8c6f514cf0345598d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4279985$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4279985$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1051127$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11457968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van den Ende, Wim</creatorcontrib><creatorcontrib>An Michiels</creatorcontrib><creatorcontrib>Dominik Van Wonterghem</creatorcontrib><creatorcontrib>Stefan P. Clerens</creatorcontrib><creatorcontrib>Joke De Roover</creatorcontrib><creatorcontrib>André J. Van Laere</creatorcontrib><title>Defoliation Induces Fructan 1-Exohydrolase II in Witloof Chicory Roots. Cloning and Purification of Two Isoforms, Fructan 1-Exohydrolase IIa and Fructan 1-Exohydrolase IIb. Mass Fingerprint of the Fructan 1-Exohydrolase II Enzymes</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be purified from forced chicory roots as well as from the etiolated "Belgian endive" leaves where the 1-FEH IIa isoform is present in higher concentrations. Full-length cDNAs were obtained by a combination of reverse transcriptase-polymerase chain reaction (PCR), PCR and 5′- and 3′-rapid amplification of cDNA ends using primers based on N-terminal and conserved amino acid sequences. 1-FEH IIa and 1-FEH IIb cDNA-derived amino acid sequences are most homologous to a new group of plant glycosyl hydrolases harboring cell wall-type enzymes with acid isoelectric points. Unlike the observed expression profiles of chicory 1-FEH I, northern analysis revealed that 1-FEH II is expressed when young chicory plants are defoliated, suggesting that this enzyme can be induced at any developmental stage when large energy supplies are necessary (regrowth after defoliation).</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>beta-Fructofuranosidase</subject><subject>Biological and medical sciences</subject><subject>Cell walls</subject><subject>Cichorium intybus</subject><subject>Cichorium intybus - enzymology</subject><subject>Cichorium intybus - genetics</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>Economic plant physiology</subject><subject>Environmental Stress and Adaptation</subject><subject>Enzymes</subject><subject>Exons</subject><subject>fructan 1-exohydrolase</subject><subject>Fructans</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoside Hydrolases - genetics</subject><subject>Glycoside Hydrolases - isolation & purification</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - isolation & purification</subject><subject>Metabolism</subject><subject>Molecular Sequence Data</subject><subject>Nutrition. Photosynthesis. Respiration. Metabolism</subject><subject>Peptide Fragments - metabolism</subject><subject>Plant Leaves - physiology</subject><subject>Plant physiology and development</subject><subject>Plant roots</subject><subject>Plant Roots - enzymology</subject><subject>Plants</subject><subject>Polymerase chain reaction</subject><subject>Protein isoforms</subject><subject>RNA</subject><subject>Sequence Homology, Amino Acid</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1DAUhSMEokNhyQ4hLxArEnwT_2XBAg1TiFQEQkUsLdexO64ydmonwPDAPAceZlTKorCyrfPdc4-lUxSPAVcAmLwcxwpqVjX5JdidYgG0qcuaEnG3WGCc71iI9qh4kNIlxhgaIPeLIwBCecvEovj5xtgwODW54FHn-1mbhE7irCflEZSr72G97WMYVDKo65Dz6IubhhAsWq6dDnGLPoUwpQoth-Cdv0DK9-jjHJ11em-a0bNvAXUp2BA36cXt7ur38K3yeYXeq5TT5TUmjtH5aWc-rc0_Aq_8j-3GpIfFPauGZB4dzuPi88nqbPmuPP3wtlu-Pi01ZWwqTSOMIS2xBlSvasIV54qRBnNua6ZqQzBueS1sX3OGldDMUiDa4oZQ2oq-OS5e7X3H-Xxjem38FNUgc9iNilsZlJN_K96t5UX4KgEY4STPPz_Mx3A1mzTJjUvaDIPyJsxJcsBAQcB_QeAtpo3AGSz3oI4hpWjsdRjActcgOY4yN0g2ctegzD-9-YM_9KEyGXh2AFTSarBRee3SDVcKUPOMPdljl2kK8VomNW9bQZtfqbLbOA</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Van den Ende, Wim</creator><creator>An Michiels</creator><creator>Dominik Van Wonterghem</creator><creator>Stefan P. Clerens</creator><creator>Joke De Roover</creator><creator>André J. Van Laere</creator><general>American Society of Plant Biologists</general><general>American Society of Plant Physiologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010701</creationdate><title>Defoliation Induces Fructan 1-Exohydrolase II in Witloof Chicory Roots. Cloning and Purification of Two Isoforms, Fructan 1-Exohydrolase IIa and Fructan 1-Exohydrolase IIb. Mass Fingerprint of the Fructan 1-Exohydrolase II Enzymes</title><author>Van den Ende, Wim ; An Michiels ; Dominik Van Wonterghem ; Stefan P. Clerens ; Joke De Roover ; André J. Van Laere</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-e38ee494fe1ada247a77a643077f26a2e4009728fd2760a8c6f514cf0345598d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>beta-Fructofuranosidase</topic><topic>Biological and medical sciences</topic><topic>Cell walls</topic><topic>Cichorium intybus</topic><topic>Cichorium intybus - enzymology</topic><topic>Cichorium intybus - genetics</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>Economic plant physiology</topic><topic>Environmental Stress and Adaptation</topic><topic>Enzymes</topic><topic>Exons</topic><topic>fructan 1-exohydrolase</topic><topic>Fructans</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoside Hydrolases - genetics</topic><topic>Glycoside Hydrolases - isolation & purification</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - isolation & purification</topic><topic>Metabolism</topic><topic>Molecular Sequence Data</topic><topic>Nutrition. Photosynthesis. Respiration. Metabolism</topic><topic>Peptide Fragments - metabolism</topic><topic>Plant Leaves - physiology</topic><topic>Plant physiology and development</topic><topic>Plant roots</topic><topic>Plant Roots - enzymology</topic><topic>Plants</topic><topic>Polymerase chain reaction</topic><topic>Protein isoforms</topic><topic>RNA</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van den Ende, Wim</creatorcontrib><creatorcontrib>An Michiels</creatorcontrib><creatorcontrib>Dominik Van Wonterghem</creatorcontrib><creatorcontrib>Stefan P. Clerens</creatorcontrib><creatorcontrib>Joke De Roover</creatorcontrib><creatorcontrib>André J. Van Laere</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van den Ende, Wim</au><au>An Michiels</au><au>Dominik Van Wonterghem</au><au>Stefan P. Clerens</au><au>Joke De Roover</au><au>André J. Van Laere</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Defoliation Induces Fructan 1-Exohydrolase II in Witloof Chicory Roots. Cloning and Purification of Two Isoforms, Fructan 1-Exohydrolase IIa and Fructan 1-Exohydrolase IIb. Mass Fingerprint of the Fructan 1-Exohydrolase II Enzymes</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>126</volume><issue>3</issue><spage>1186</spage><epage>1195</epage><pages>1186-1195</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be purified from forced chicory roots as well as from the etiolated "Belgian endive" leaves where the 1-FEH IIa isoform is present in higher concentrations. Full-length cDNAs were obtained by a combination of reverse transcriptase-polymerase chain reaction (PCR), PCR and 5′- and 3′-rapid amplification of cDNA ends using primers based on N-terminal and conserved amino acid sequences. 1-FEH IIa and 1-FEH IIb cDNA-derived amino acid sequences are most homologous to a new group of plant glycosyl hydrolases harboring cell wall-type enzymes with acid isoelectric points. Unlike the observed expression profiles of chicory 1-FEH I, northern analysis revealed that 1-FEH II is expressed when young chicory plants are defoliated, suggesting that this enzyme can be induced at any developmental stage when large energy supplies are necessary (regrowth after defoliation).</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>11457968</pmid><doi>10.1104/pp.126.3.1186</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 2001-07, Vol.126 (3), p.1186-1195 |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Agronomy. Soil science and plant productions Amino Acid Sequence Amino acids beta-Fructofuranosidase Biological and medical sciences Cell walls Cichorium intybus Cichorium intybus - enzymology Cichorium intybus - genetics Cloning, Molecular Complementary DNA Economic plant physiology Environmental Stress and Adaptation Enzymes Exons fructan 1-exohydrolase Fructans Fundamental and applied biological sciences. Psychology Glycoside Hydrolases - genetics Glycoside Hydrolases - isolation & purification Isoenzymes - genetics Isoenzymes - isolation & purification Metabolism Molecular Sequence Data Nutrition. Photosynthesis. Respiration. Metabolism Peptide Fragments - metabolism Plant Leaves - physiology Plant physiology and development Plant roots Plant Roots - enzymology Plants Polymerase chain reaction Protein isoforms RNA Sequence Homology, Amino Acid |
| title | Defoliation Induces Fructan 1-Exohydrolase II in Witloof Chicory Roots. Cloning and Purification of Two Isoforms, Fructan 1-Exohydrolase IIa and Fructan 1-Exohydrolase IIb. Mass Fingerprint of the Fructan 1-Exohydrolase II Enzymes |
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