The relationship between controlled ovarian stimulation protocol, meiotic spindle visibility, position of the meiotic spindle relative to the polar body in the human oocyte, and clinical outcomes following ICSI
Purpose To investigate the effects of different controlled ovarian stimulation (COS) protocols, including the progestin‐primed ovarian stimulation (PPOS), long, short, and the gonadotropin‐releasing hormone antagonist protocols, on meiotic spindle visibility and position within the oocyte and clinic...
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| Veröffentlicht in: | Reproductive medicine and biology 2024-01, Vol.23 (1), p.e12601-n/a |
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| creator | Inoue, Taketo Matsuo, Yuki Taguchi, Sayumi Tsujimoto, Yoshiko Uemura, Mikiko Yamashita, Yoshiki |
| description | Purpose
To investigate the effects of different controlled ovarian stimulation (COS) protocols, including the progestin‐primed ovarian stimulation (PPOS), long, short, and the gonadotropin‐releasing hormone antagonist protocols, on meiotic spindle visibility and position within the oocyte and clinical outcomes following ICSI.
Methods
Before ICSI, spindle position (θ) just below the polar body (PB) was defined as 0° and categorized as follows: θ = 0°, 0° |
| doi_str_mv | 10.1002/rmb2.12601 |
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To investigate the effects of different controlled ovarian stimulation (COS) protocols, including the progestin‐primed ovarian stimulation (PPOS), long, short, and the gonadotropin‐releasing hormone antagonist protocols, on meiotic spindle visibility and position within the oocyte and clinical outcomes following ICSI.
Methods
Before ICSI, spindle position (θ) just below the polar body (PB) was defined as 0° and categorized as follows: θ = 0°, 0° < θ ≤ 30°, 30° < θ ≤ 60°, 60° < θ ≤ 90°, 90° < θ ≤ 180°, between the PB and the oolemma, and nonvisible. The clinical outcomes after ICSI were retrospectively analyzed.
Results
The normal fertilization rate was significantly higher in oocytes with visible spindles than in oocytes with nonvisible spindles after each COS protocol, but did not differ based on spindle positioning (0° ≤ θ ≤ 180°). The rates of pregnancy, live birth/ongoing pregnancy, and miscarriage did not differ based on spindle visibility or positioning. In multinominal logistic regression analysis, female age was associated with spindle position, and the odds of a spindle located at 30° < θ ≤ 60°, at 60° < θ ≤ 90°, or at 90° < θ ≤ 180° were increased relative to θ = 0° in older women (odds ratio; 1.020, 1.030, and 1.060, respectively; p < 0.05).
Conclusion
Meiotic spindle positioning in the oocyte does not affect normal fertilization, blastulation, pregnancy, live birth/ongoing pregnancy, and miscarriage after ICSI, independent of the COS protocol used.
The visibility of the meiotic spindle in oocytes impacted the normal fertilization rate, independent of controlled ovarian stimulation protocol. However, the spindle position within the ooplasm did not influence clinical outcomes, such as normal fertilization, blastulation, pregnancy, live birth, and miscarriage after controlled ovarian stimulation. Furthermore, female age did not affect spindle visibility; whereas the spindle position was associated with female age and moved away from directly beneath the polar body as female age increased.]]></description><identifier>ISSN: 1445-5781</identifier><identifier>EISSN: 1447-0578</identifier><identifier>DOI: 10.1002/rmb2.12601</identifier><identifier>PMID: 39677331</identifier><language>eng</language><publisher>Japan: John Wiley & Sons, Inc</publisher><subject>Age ; Clinical outcomes ; controlled ovarian stimulation ; female age ; Females ; Fertilization ; Gonadotropin ; Gonadotropins ; Hormone antagonists ; Hydroxyprogesterone ; intracytoplasmic sperm injection ; Meiosis ; Miscarriage ; Neurohormones ; Oocytes ; Original ; Ovaries ; Patients ; Pituitary (anterior) ; Pregnancy ; Progestin ; Regression analysis ; Sperm ; spindle ; Spindles ; Statistical analysis ; visibility</subject><ispartof>Reproductive medicine and biology, 2024-01, Vol.23 (1), p.e12601-n/a</ispartof><rights>2024 The Author(s). published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.</rights><rights>2024 The Author(s). Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.</rights><rights>COPYRIGHT 2024 John Wiley & Sons, Inc.</rights><rights>2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4351-a8047b4a0c4a9ccf186f69b05b6b690d8a874df7800e005439160367ebbff1953</cites><orcidid>0000-0003-1988-4304 ; 0000-0001-8461-7355 ; 0009-0003-9400-1126 ; 0000-0002-9605-2379</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645447/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645447/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,1416,11560,27922,27923,45572,45573,46050,46474,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39677331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inoue, Taketo</creatorcontrib><creatorcontrib>Matsuo, Yuki</creatorcontrib><creatorcontrib>Taguchi, Sayumi</creatorcontrib><creatorcontrib>Tsujimoto, Yoshiko</creatorcontrib><creatorcontrib>Uemura, Mikiko</creatorcontrib><creatorcontrib>Yamashita, Yoshiki</creatorcontrib><title>The relationship between controlled ovarian stimulation protocol, meiotic spindle visibility, position of the meiotic spindle relative to the polar body in the human oocyte, and clinical outcomes following ICSI</title><title>Reproductive medicine and biology</title><addtitle>Reprod Med Biol</addtitle><description><![CDATA[Purpose
To investigate the effects of different controlled ovarian stimulation (COS) protocols, including the progestin‐primed ovarian stimulation (PPOS), long, short, and the gonadotropin‐releasing hormone antagonist protocols, on meiotic spindle visibility and position within the oocyte and clinical outcomes following ICSI.
Methods
Before ICSI, spindle position (θ) just below the polar body (PB) was defined as 0° and categorized as follows: θ = 0°, 0° < θ ≤ 30°, 30° < θ ≤ 60°, 60° < θ ≤ 90°, 90° < θ ≤ 180°, between the PB and the oolemma, and nonvisible. The clinical outcomes after ICSI were retrospectively analyzed.
Results
The normal fertilization rate was significantly higher in oocytes with visible spindles than in oocytes with nonvisible spindles after each COS protocol, but did not differ based on spindle positioning (0° ≤ θ ≤ 180°). The rates of pregnancy, live birth/ongoing pregnancy, and miscarriage did not differ based on spindle visibility or positioning. In multinominal logistic regression analysis, female age was associated with spindle position, and the odds of a spindle located at 30° < θ ≤ 60°, at 60° < θ ≤ 90°, or at 90° < θ ≤ 180° were increased relative to θ = 0° in older women (odds ratio; 1.020, 1.030, and 1.060, respectively; p < 0.05).
Conclusion
Meiotic spindle positioning in the oocyte does not affect normal fertilization, blastulation, pregnancy, live birth/ongoing pregnancy, and miscarriage after ICSI, independent of the COS protocol used.
The visibility of the meiotic spindle in oocytes impacted the normal fertilization rate, independent of controlled ovarian stimulation protocol. However, the spindle position within the ooplasm did not influence clinical outcomes, such as normal fertilization, blastulation, pregnancy, live birth, and miscarriage after controlled ovarian stimulation. Furthermore, female age did not affect spindle visibility; whereas the spindle position was associated with female age and moved away from directly beneath the polar body as female age increased.]]></description><subject>Age</subject><subject>Clinical outcomes</subject><subject>controlled ovarian stimulation</subject><subject>female age</subject><subject>Females</subject><subject>Fertilization</subject><subject>Gonadotropin</subject><subject>Gonadotropins</subject><subject>Hormone antagonists</subject><subject>Hydroxyprogesterone</subject><subject>intracytoplasmic sperm injection</subject><subject>Meiosis</subject><subject>Miscarriage</subject><subject>Neurohormones</subject><subject>Oocytes</subject><subject>Original</subject><subject>Ovaries</subject><subject>Patients</subject><subject>Pituitary (anterior)</subject><subject>Pregnancy</subject><subject>Progestin</subject><subject>Regression analysis</subject><subject>Sperm</subject><subject>spindle</subject><subject>Spindles</subject><subject>Statistical analysis</subject><subject>visibility</subject><issn>1445-5781</issn><issn>1447-0578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNp9ktuO0zAURSMEYobCCx-ALPGCUFvsXJ0nNFRcKg1CguHZcnxpXTk-wU5a9Tf5ItxkGOgIoUSKdby8d87xTpLnBC8Jxukb3zbpkqQlJg-SS5Ln1QIXFX04rotFXJKL5EkIO4xJVefp4-Qiq8uqyjJymfy82SrkleW9ARe2pkON6g9KOSTA9R6sVRLBnnvDHQq9aYcJRZ2HHgTYOWqVgd4IFDrjpFVob4JpjDX9cY46CGbEQaM-Ot1nJ-e9Qj2M-x1Y7lED8oiMGyvboY3OAOLYqzniTiJhjTOCWwRDL6BVAen4m3AwboPWq2_rp8kjzW1Qz26_s-T7h_c3q0-L6y8f16ur64XIs4IsOMV51eQci5zXQmhCS13WDS6asilrLCmnVS51RTFWGBd5VpMSZ2WlmkZrUhfZLFlPuhL4jnXetNwfGXDDxgL4DeM-NmsV45gXlaSUailzXJOaZIRLkmoS76HJ6qj1dtLqhqZVUqg4e27PRM93nNmyDewZIWVexCuPCq9uFTz8GFToWWuCUNZyp2AILCN5SQuC6zSiL--hOxi8i7M6UVVaUpzSP9SGxw6M0xCNxUmUXdEUl5Rk8Z0ly39Q8ZGqNTFCSptYPzvwejogPITglb5rkmB2ijM7xZmNcY7wi7_Hcof-zm8EyAQcos3xP1Ls6-d36ST6C9GOAoI</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Inoue, Taketo</creator><creator>Matsuo, Yuki</creator><creator>Taguchi, Sayumi</creator><creator>Tsujimoto, Yoshiko</creator><creator>Uemura, Mikiko</creator><creator>Yamashita, Yoshiki</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><general>Wiley</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1988-4304</orcidid><orcidid>https://orcid.org/0000-0001-8461-7355</orcidid><orcidid>https://orcid.org/0009-0003-9400-1126</orcidid><orcidid>https://orcid.org/0000-0002-9605-2379</orcidid></search><sort><creationdate>202401</creationdate><title>The relationship between controlled ovarian stimulation protocol, meiotic spindle visibility, position of the meiotic spindle relative to the polar body in the human oocyte, and clinical outcomes following ICSI</title><author>Inoue, Taketo ; Matsuo, Yuki ; Taguchi, Sayumi ; Tsujimoto, Yoshiko ; Uemura, Mikiko ; Yamashita, Yoshiki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4351-a8047b4a0c4a9ccf186f69b05b6b690d8a874df7800e005439160367ebbff1953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Age</topic><topic>Clinical outcomes</topic><topic>controlled ovarian stimulation</topic><topic>female age</topic><topic>Females</topic><topic>Fertilization</topic><topic>Gonadotropin</topic><topic>Gonadotropins</topic><topic>Hormone antagonists</topic><topic>Hydroxyprogesterone</topic><topic>intracytoplasmic sperm injection</topic><topic>Meiosis</topic><topic>Miscarriage</topic><topic>Neurohormones</topic><topic>Oocytes</topic><topic>Original</topic><topic>Ovaries</topic><topic>Patients</topic><topic>Pituitary (anterior)</topic><topic>Pregnancy</topic><topic>Progestin</topic><topic>Regression analysis</topic><topic>Sperm</topic><topic>spindle</topic><topic>Spindles</topic><topic>Statistical analysis</topic><topic>visibility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inoue, Taketo</creatorcontrib><creatorcontrib>Matsuo, Yuki</creatorcontrib><creatorcontrib>Taguchi, Sayumi</creatorcontrib><creatorcontrib>Tsujimoto, Yoshiko</creatorcontrib><creatorcontrib>Uemura, Mikiko</creatorcontrib><creatorcontrib>Yamashita, Yoshiki</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Online Library Free Content</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Reproductive medicine and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inoue, Taketo</au><au>Matsuo, Yuki</au><au>Taguchi, Sayumi</au><au>Tsujimoto, Yoshiko</au><au>Uemura, Mikiko</au><au>Yamashita, Yoshiki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The relationship between controlled ovarian stimulation protocol, meiotic spindle visibility, position of the meiotic spindle relative to the polar body in the human oocyte, and clinical outcomes following ICSI</atitle><jtitle>Reproductive medicine and biology</jtitle><addtitle>Reprod Med Biol</addtitle><date>2024-01</date><risdate>2024</risdate><volume>23</volume><issue>1</issue><spage>e12601</spage><epage>n/a</epage><pages>e12601-n/a</pages><issn>1445-5781</issn><eissn>1447-0578</eissn><abstract><![CDATA[Purpose
To investigate the effects of different controlled ovarian stimulation (COS) protocols, including the progestin‐primed ovarian stimulation (PPOS), long, short, and the gonadotropin‐releasing hormone antagonist protocols, on meiotic spindle visibility and position within the oocyte and clinical outcomes following ICSI.
Methods
Before ICSI, spindle position (θ) just below the polar body (PB) was defined as 0° and categorized as follows: θ = 0°, 0° < θ ≤ 30°, 30° < θ ≤ 60°, 60° < θ ≤ 90°, 90° < θ ≤ 180°, between the PB and the oolemma, and nonvisible. The clinical outcomes after ICSI were retrospectively analyzed.
Results
The normal fertilization rate was significantly higher in oocytes with visible spindles than in oocytes with nonvisible spindles after each COS protocol, but did not differ based on spindle positioning (0° ≤ θ ≤ 180°). The rates of pregnancy, live birth/ongoing pregnancy, and miscarriage did not differ based on spindle visibility or positioning. In multinominal logistic regression analysis, female age was associated with spindle position, and the odds of a spindle located at 30° < θ ≤ 60°, at 60° < θ ≤ 90°, or at 90° < θ ≤ 180° were increased relative to θ = 0° in older women (odds ratio; 1.020, 1.030, and 1.060, respectively; p < 0.05).
Conclusion
Meiotic spindle positioning in the oocyte does not affect normal fertilization, blastulation, pregnancy, live birth/ongoing pregnancy, and miscarriage after ICSI, independent of the COS protocol used.
The visibility of the meiotic spindle in oocytes impacted the normal fertilization rate, independent of controlled ovarian stimulation protocol. However, the spindle position within the ooplasm did not influence clinical outcomes, such as normal fertilization, blastulation, pregnancy, live birth, and miscarriage after controlled ovarian stimulation. Furthermore, female age did not affect spindle visibility; whereas the spindle position was associated with female age and moved away from directly beneath the polar body as female age increased.]]></abstract><cop>Japan</cop><pub>John Wiley & Sons, Inc</pub><pmid>39677331</pmid><doi>10.1002/rmb2.12601</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0003-1988-4304</orcidid><orcidid>https://orcid.org/0000-0001-8461-7355</orcidid><orcidid>https://orcid.org/0009-0003-9400-1126</orcidid><orcidid>https://orcid.org/0000-0002-9605-2379</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; Wiley-Blackwell Open Access Titles; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Age Clinical outcomes controlled ovarian stimulation female age Females Fertilization Gonadotropin Gonadotropins Hormone antagonists Hydroxyprogesterone intracytoplasmic sperm injection Meiosis Miscarriage Neurohormones Oocytes Original Ovaries Patients Pituitary (anterior) Pregnancy Progestin Regression analysis Sperm spindle Spindles Statistical analysis visibility |
| title | The relationship between controlled ovarian stimulation protocol, meiotic spindle visibility, position of the meiotic spindle relative to the polar body in the human oocyte, and clinical outcomes following ICSI |
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