The chemical constitution of the proteoglycan of human intervertebral disc

Proteoglycan was prepared from three pools of normal human intervertebral discs by extraction with buffered 4M-guanidinium chloride followed by CsCl-density-gradient ultracentrifugation. Chromatography on agarose (Bio-Gel A-150m) and on DEAE-cellulose suggested a single polydisperse proteoglycan spe...

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Veröffentlicht in:	Biochemical journal 1976-09, Vol.157 (3), p.753-763
Hauptverfasser:	Pearce, R H, Grimmer, B J
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Chondroitin Sulfates - analysis Glycosaminoglycans - isolation & purification Hexosamines - analysis Hexoses - analysis Hexuronic Acids - analysis Humans Intervertebral Disc - analysis Keratan Sulfate - analysis Male Middle Aged Molecular Weight Nitrogen - analysis Proteoglycans - analysis Viscosity
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