Studies on the assembly of the rat lens capsule. Biosynthesis of a cross-linked collagenous component of high molecular weight

Studies on the assembly of the rat lens capsule. Biosynthesis of a cross-linked collagenous component of high molecular weight

1. Intact rat lenses in tissue culture synthesize hydroxy[3H]proline-containing polypeptides of apparent mol.wt. approx. 180000, which become assembled into aggregates of higher molecular weight with time. 2. Both the 180000-mol.wt. species and the aggregates are components of the deoxycholate-insol...

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Veröffentlicht in:Biochemical journal 1980-08, Vol.190 (2), p.229-237
Hauptverfasser: Heathcote, J G, Bailey, A J, Grant, M E
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Borohydrides - pharmacology
Chromatography, Gel
Collagen - biosynthesis
Culture Techniques
Dipeptides - biosynthesis
Lens, Crystalline - drug effects
Lens, Crystalline - embryology
Lens, Crystalline - metabolism
Male
Molecular Weight
Oxidation-Reduction
Rats
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container_title Biochemical journal
container_volume 190
creator Heathcote, J G
Bailey, A J
Grant, M E
description 1. Intact rat lenses in tissue culture synthesize hydroxy[3H]proline-containing polypeptides of apparent mol.wt. approx. 180000, which become assembled into aggregates of higher molecular weight with time. 2. Both the 180000-mol.wt. species and the aggregates are components of the deoxycholate-insoluble base-membrane matrix. 3. Formation of the high-molecular-weight aggregate is accompanied by the biosynthesis of the reducible hydroxylysine-derived cross-link hydroxylysino-5-oxo-norleucine. 4. Hydroxylysino-5-oxonorleucine and dehydrohydroxylysinonorleucine are the major reducible cross-links present in intact foetal and 1-month-old calf lens capsules.
doi_str_mv 10.1042/bj1900229
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source MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection
subjects Animals
Borohydrides - pharmacology
Chromatography, Gel
Collagen - biosynthesis
Culture Techniques
Dipeptides - biosynthesis
Lens, Crystalline - drug effects
Lens, Crystalline - embryology
Lens, Crystalline - metabolism
Male
Molecular Weight
Oxidation-Reduction
Rats
title Studies on the assembly of the rat lens capsule. Biosynthesis of a cross-linked collagenous component of high molecular weight
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