Subcellular distribution of enzymes determined by rapid digitonin fractionation of isolated hepatocytes

Conditions were determined for rapid separation of cytosolic and mitochondrial compartments by digitonin fractionation of rat hepatocytes. The minimum time required for separation of mitochondrial and cytosolic enzyme markers decreased rapidly with increasing temperature. Kyro EOB, a non-ionic deter...

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Veröffentlicht in:	Biochemical journal 1980-02, Vol.186 (2), p.423-429
Hauptverfasser:	Janski, A M, Cornell, N W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Fractionation - methods Detergents Digitonin In Vitro Techniques Liver - cytology Liver - enzymology Male Organic Chemicals Rats Starvation - enzymology Subcellular Fractions - enzymology Subcellular Structures Temperature
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