Transport of glutamine by rat kidney brush-border membrane vesicles
Transport of glutamine by brush-border vesicles prepared from the renal cortex was studied. The transport system had both Na+-dependent and Na+-independent components. The presence of Na+ in the incubation resulted in an 'overshoot' at 30s at which time the rates of transport were approx....
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| Veröffentlicht in: | Biochemical journal 1979-08, Vol.182 (2), p.295-300 |
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| creator | McFarlane-Anderson, N Alleyne, G A |
| description | Transport of glutamine by brush-border vesicles prepared from the renal cortex was studied. The transport system had both Na+-dependent and Na+-independent components. The presence of Na+ in the incubation resulted in an 'overshoot' at 30s at which time the rates of transport were approx. 8 times the values obtained in the absence of Na+. Variation of the glutamine concentration showed that the system obeyed Michaelis-Menten kinetics with Km and Vmax. values for the Na+-dependent system of 0.86 mM and 9.6 nmol/min per mg of protein respectively. Vesicles obtained from chronically acidotic rats showed similar kinetic characteristics. The Km and Vmax. values for the Na+-dependent system were 0.76 mM and 9.6 nmol/min per mg of protein respectively. There was increased uptake of glutamine by vesicles from acidotic rats and this increase was associated with increased activity of gamma-glutamyltransferase in these preparations. Vesicles from acidotic rats, however, showed no increase in glucose transport and no increase in the activity of maltase, another brush-border enzyme. |
| doi_str_mv | 10.1042/bj1820295 |
| format | Article |
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The transport system had both Na+-dependent and Na+-independent components. The presence of Na+ in the incubation resulted in an 'overshoot' at 30s at which time the rates of transport were approx. 8 times the values obtained in the absence of Na+. Variation of the glutamine concentration showed that the system obeyed Michaelis-Menten kinetics with Km and Vmax. values for the Na+-dependent system of 0.86 mM and 9.6 nmol/min per mg of protein respectively. Vesicles obtained from chronically acidotic rats showed similar kinetic characteristics. The Km and Vmax. values for the Na+-dependent system were 0.76 mM and 9.6 nmol/min per mg of protein respectively. There was increased uptake of glutamine by vesicles from acidotic rats and this increase was associated with increased activity of gamma-glutamyltransferase in these preparations. Vesicles from acidotic rats, however, showed no increase in glucose transport and no increase in the activity of maltase, another brush-border enzyme.</description><identifier>ISSN: 0264-6021</identifier><identifier>ISSN: 0306-3283</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj1820295</identifier><identifier>PMID: 41516</identifier><language>eng</language><publisher>England</publisher><subject>Acidosis - metabolism ; Animals ; Biological Transport ; Cell Membrane - metabolism ; gamma-Glutamyltransferase - metabolism ; Glutamine - metabolism ; Kidney Cortex - metabolism ; Kidney Cortex - ultrastructure ; Kinetics ; Male ; Microvilli - metabolism ; Microvilli - ultrastructure ; Rats ; Sodium - metabolism</subject><ispartof>Biochemical journal, 1979-08, Vol.182 (2), p.295-300</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-8a442ec2df1eee6b9ee339f599b916c884a27555472ec861f04a190de70002813</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1161307/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1161307/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/41516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McFarlane-Anderson, N</creatorcontrib><creatorcontrib>Alleyne, G A</creatorcontrib><title>Transport of glutamine by rat kidney brush-border membrane vesicles</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>Transport of glutamine by brush-border vesicles prepared from the renal cortex was studied. The transport system had both Na+-dependent and Na+-independent components. The presence of Na+ in the incubation resulted in an 'overshoot' at 30s at which time the rates of transport were approx. 8 times the values obtained in the absence of Na+. Variation of the glutamine concentration showed that the system obeyed Michaelis-Menten kinetics with Km and Vmax. values for the Na+-dependent system of 0.86 mM and 9.6 nmol/min per mg of protein respectively. Vesicles obtained from chronically acidotic rats showed similar kinetic characteristics. The Km and Vmax. values for the Na+-dependent system were 0.76 mM and 9.6 nmol/min per mg of protein respectively. There was increased uptake of glutamine by vesicles from acidotic rats and this increase was associated with increased activity of gamma-glutamyltransferase in these preparations. Vesicles from acidotic rats, however, showed no increase in glucose transport and no increase in the activity of maltase, another brush-border enzyme.</description><subject>Acidosis - metabolism</subject><subject>Animals</subject><subject>Biological Transport</subject><subject>Cell Membrane - metabolism</subject><subject>gamma-Glutamyltransferase - metabolism</subject><subject>Glutamine - metabolism</subject><subject>Kidney Cortex - metabolism</subject><subject>Kidney Cortex - ultrastructure</subject><subject>Kinetics</subject><subject>Male</subject><subject>Microvilli - metabolism</subject><subject>Microvilli - ultrastructure</subject><subject>Rats</subject><subject>Sodium - metabolism</subject><issn>0264-6021</issn><issn>0306-3283</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkDtPwzAUhS1EBaUwMLN4ZQjc6ziOsyChipdUiaXMlp3ctCl5VHZSqf-eQFEF0x3Od74rHcauEe4QpLh3G9QCRJacsCnKFCKdCn3KpiCUjBQIPGcXIWwAUIKEMzaRmKCasvnS2zZsO9_zruSreuhtU7XE3Z572_PPqmhpz50fwjpynS_I84YaN5aI7yhUeU3hkk1KWwe6-r0z9vH8tJy_Rov3l7f54yLK4zjtI22lFJSLokQiUi4jiuOsTLLMZahyraUVaZIkMh0prbAEaTGDglIAEBrjGXs4eLeDa6jIqe29rc3WV431e9PZyvxP2mptVt3OICqMIR0FtwdB7rsQPJXHLoL5ntEcZxzZm7_PjuTPbvEXiQVt-A</recordid><startdate>19790815</startdate><enddate>19790815</enddate><creator>McFarlane-Anderson, N</creator><creator>Alleyne, G A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19790815</creationdate><title>Transport of glutamine by rat kidney brush-border membrane vesicles</title><author>McFarlane-Anderson, N ; Alleyne, G A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-8a442ec2df1eee6b9ee339f599b916c884a27555472ec861f04a190de70002813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Acidosis - metabolism</topic><topic>Animals</topic><topic>Biological Transport</topic><topic>Cell Membrane - metabolism</topic><topic>gamma-Glutamyltransferase - metabolism</topic><topic>Glutamine - metabolism</topic><topic>Kidney Cortex - metabolism</topic><topic>Kidney Cortex - ultrastructure</topic><topic>Kinetics</topic><topic>Male</topic><topic>Microvilli - metabolism</topic><topic>Microvilli - ultrastructure</topic><topic>Rats</topic><topic>Sodium - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McFarlane-Anderson, N</creatorcontrib><creatorcontrib>Alleyne, G A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McFarlane-Anderson, N</au><au>Alleyne, G A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transport of glutamine by rat kidney brush-border membrane vesicles</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1979-08-15</date><risdate>1979</risdate><volume>182</volume><issue>2</issue><spage>295</spage><epage>300</epage><pages>295-300</pages><issn>0264-6021</issn><issn>0306-3283</issn><eissn>1470-8728</eissn><abstract>Transport of glutamine by brush-border vesicles prepared from the renal cortex was studied. The transport system had both Na+-dependent and Na+-independent components. The presence of Na+ in the incubation resulted in an 'overshoot' at 30s at which time the rates of transport were approx. 8 times the values obtained in the absence of Na+. Variation of the glutamine concentration showed that the system obeyed Michaelis-Menten kinetics with Km and Vmax. values for the Na+-dependent system of 0.86 mM and 9.6 nmol/min per mg of protein respectively. Vesicles obtained from chronically acidotic rats showed similar kinetic characteristics. The Km and Vmax. values for the Na+-dependent system were 0.76 mM and 9.6 nmol/min per mg of protein respectively. There was increased uptake of glutamine by vesicles from acidotic rats and this increase was associated with increased activity of gamma-glutamyltransferase in these preparations. Vesicles from acidotic rats, however, showed no increase in glucose transport and no increase in the activity of maltase, another brush-border enzyme.</abstract><cop>England</cop><pmid>41516</pmid><doi>10.1042/bj1820295</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0264-6021 |
| ispartof | Biochemical journal, 1979-08, Vol.182 (2), p.295-300 |
| issn | 0264-6021 0306-3283 1470-8728 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Acidosis - metabolism Animals Biological Transport Cell Membrane - metabolism gamma-Glutamyltransferase - metabolism Glutamine - metabolism Kidney Cortex - metabolism Kidney Cortex - ultrastructure Kinetics Male Microvilli - metabolism Microvilli - ultrastructure Rats Sodium - metabolism |
| title | Transport of glutamine by rat kidney brush-border membrane vesicles |
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