Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor
Metabolic flexibility of skeletal muscle, that is, the preference for fat oxidation (FOx) during fasting and for carbohydrate oxidation in response to insulin, is decreased during insulin resistance. The aim of this study was to test the hypothesis that the capacity of myotubes to oxidize fat in vit...
Gespeichert in:
| Veröffentlicht in: | The Journal of clinical investigation 2005-07, Vol.115 (7), p.1934-1941 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1941 |
|---|---|
| container_issue | 7 |
| container_start_page | 1934 |
| container_title | The Journal of clinical investigation |
| container_volume | 115 |
| creator | Ukropcova, Barbara McNeil, Michele Sereda, Olga de Jonge, Lilian Xie, Hui Bray, George A Smith, Steven R |
| description | Metabolic flexibility of skeletal muscle, that is, the preference for fat oxidation (FOx) during fasting and for carbohydrate oxidation in response to insulin, is decreased during insulin resistance. The aim of this study was to test the hypothesis that the capacity of myotubes to oxidize fat in vitro reflects the donor's metabolic characteristics. Insulin sensitivity (IS) and metabolic flexibility of 16 healthy, young male subjects was determined by euglycemic hyperinsulinemic clamp. Muscle samples were obtained from vastus lateralis, cultured, and differentiated into myotubes. In human myotubes in vitro, we measured suppressibility (glucose suppression of FOx) and adaptability (an increase in FOx in the presence of high palmitate concentration). We termed these dynamic changes in FOx metabolic switching. In vivo, metabolic flexibility was positively correlated with IS and maximal oxygen uptake and inversely correlated with percent body fat. In vitro suppressibility was inversely correlated with IS and metabolic flexibility and positively correlated with body fat and fasting FFA levels. Adaptability was negatively associated with percent body fat and fasting insulin and positively correlated with IS and metabolic flexibility. The interindividual variability in metabolic phenotypes was preserved in human myotubes separated from their neuroendocrine environment, which supports the hypothesis that metabolic switching is an intrinsic property of skeletal muscle. |
| doi_str_mv | 10.1172/jci24332 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1159139</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68016895</sourcerecordid><originalsourceid>FETCH-LOGICAL-c463t-5d724d0fa2648d016c7a2455a12f08bd1a3ac5a8fb3a1af5a07a365f09a2f6673</originalsourceid><addsrcrecordid>eNpdkU1r3DAQhkVpaTZpIb-giB5CLm71bfkSCNuvlEAu7VnMylKsxZYSSS7Zf18nuzRtTwMzDw_v8CJ0SskHSlv2cWsDE5yzF2hFpdSNZly_RCtCGG26lusjdFzKlhAqhBSv0RFVhLRMqhUaPu0iTMFiO0C8dQWHiD1UnB5CDzWk-LgY5gkivsthgrzD0y7ZXV3QKeScMp5chU0a944MtrocSg224ORxHRzuU0z5DXrlYSzu7WGeoJ9fPv9Yf2uub75erS-vGysUr43sWyZ64oEpoXtClW2BCSmBMk_0pqfAwUrQfsOBgpdAWuBKetIB80q1_ARd7L1382ZyvXWxZhjNIbxJEMy_lxgGc5t-GUplR3m3CM4OgpzuZ1eqmUKxbhwhujQXo_SSSndyAd__B27TnOPynGGESC65EAt0vodsTqVk5_8kocQ8dme-r6-eulvQd38nfwYPZfHfLLCWXw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>200535344</pqid></control><display><type>article</type><title>Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Ukropcova, Barbara ; McNeil, Michele ; Sereda, Olga ; de Jonge, Lilian ; Xie, Hui ; Bray, George A ; Smith, Steven R</creator><creatorcontrib>Ukropcova, Barbara ; McNeil, Michele ; Sereda, Olga ; de Jonge, Lilian ; Xie, Hui ; Bray, George A ; Smith, Steven R</creatorcontrib><description>Metabolic flexibility of skeletal muscle, that is, the preference for fat oxidation (FOx) during fasting and for carbohydrate oxidation in response to insulin, is decreased during insulin resistance. The aim of this study was to test the hypothesis that the capacity of myotubes to oxidize fat in vitro reflects the donor's metabolic characteristics. Insulin sensitivity (IS) and metabolic flexibility of 16 healthy, young male subjects was determined by euglycemic hyperinsulinemic clamp. Muscle samples were obtained from vastus lateralis, cultured, and differentiated into myotubes. In human myotubes in vitro, we measured suppressibility (glucose suppression of FOx) and adaptability (an increase in FOx in the presence of high palmitate concentration). We termed these dynamic changes in FOx metabolic switching. In vivo, metabolic flexibility was positively correlated with IS and maximal oxygen uptake and inversely correlated with percent body fat. In vitro suppressibility was inversely correlated with IS and metabolic flexibility and positively correlated with body fat and fasting FFA levels. Adaptability was negatively associated with percent body fat and fasting insulin and positively correlated with IS and metabolic flexibility. The interindividual variability in metabolic phenotypes was preserved in human myotubes separated from their neuroendocrine environment, which supports the hypothesis that metabolic switching is an intrinsic property of skeletal muscle.</description><identifier>ISSN: 0021-9738</identifier><identifier>EISSN: 1558-8238</identifier><identifier>DOI: 10.1172/jci24332</identifier><identifier>PMID: 16007256</identifier><language>eng</language><publisher>United States: American Society for Clinical Investigation</publisher><subject>Adolescent ; Adult ; Biomedical research ; Dietary Fats - metabolism ; Glucose - metabolism ; Glucose Clamp Technique ; Humans ; In Vitro Techniques ; Insulin Resistance ; Male ; Models, Biological ; Muscle Fibers, Skeletal - metabolism ; Oxidation-Reduction ; Palmitic Acid - metabolism ; Phenotype</subject><ispartof>The Journal of clinical investigation, 2005-07, Vol.115 (7), p.1934-1941</ispartof><rights>Copyright American Society for Clinical Investigation Jul 2005</rights><rights>Copyright © 2005, American Society for Clinical Investigation 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-5d724d0fa2648d016c7a2455a12f08bd1a3ac5a8fb3a1af5a07a365f09a2f6673</citedby><cites>FETCH-LOGICAL-c463t-5d724d0fa2648d016c7a2455a12f08bd1a3ac5a8fb3a1af5a07a365f09a2f6673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1159139/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1159139/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27911,27912,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16007256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ukropcova, Barbara</creatorcontrib><creatorcontrib>McNeil, Michele</creatorcontrib><creatorcontrib>Sereda, Olga</creatorcontrib><creatorcontrib>de Jonge, Lilian</creatorcontrib><creatorcontrib>Xie, Hui</creatorcontrib><creatorcontrib>Bray, George A</creatorcontrib><creatorcontrib>Smith, Steven R</creatorcontrib><title>Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor</title><title>The Journal of clinical investigation</title><addtitle>J Clin Invest</addtitle><description>Metabolic flexibility of skeletal muscle, that is, the preference for fat oxidation (FOx) during fasting and for carbohydrate oxidation in response to insulin, is decreased during insulin resistance. The aim of this study was to test the hypothesis that the capacity of myotubes to oxidize fat in vitro reflects the donor's metabolic characteristics. Insulin sensitivity (IS) and metabolic flexibility of 16 healthy, young male subjects was determined by euglycemic hyperinsulinemic clamp. Muscle samples were obtained from vastus lateralis, cultured, and differentiated into myotubes. In human myotubes in vitro, we measured suppressibility (glucose suppression of FOx) and adaptability (an increase in FOx in the presence of high palmitate concentration). We termed these dynamic changes in FOx metabolic switching. In vivo, metabolic flexibility was positively correlated with IS and maximal oxygen uptake and inversely correlated with percent body fat. In vitro suppressibility was inversely correlated with IS and metabolic flexibility and positively correlated with body fat and fasting FFA levels. Adaptability was negatively associated with percent body fat and fasting insulin and positively correlated with IS and metabolic flexibility. The interindividual variability in metabolic phenotypes was preserved in human myotubes separated from their neuroendocrine environment, which supports the hypothesis that metabolic switching is an intrinsic property of skeletal muscle.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biomedical research</subject><subject>Dietary Fats - metabolism</subject><subject>Glucose - metabolism</subject><subject>Glucose Clamp Technique</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Insulin Resistance</subject><subject>Male</subject><subject>Models, Biological</subject><subject>Muscle Fibers, Skeletal - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Palmitic Acid - metabolism</subject><subject>Phenotype</subject><issn>0021-9738</issn><issn>1558-8238</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU1r3DAQhkVpaTZpIb-giB5CLm71bfkSCNuvlEAu7VnMylKsxZYSSS7Zf18nuzRtTwMzDw_v8CJ0SskHSlv2cWsDE5yzF2hFpdSNZly_RCtCGG26lusjdFzKlhAqhBSv0RFVhLRMqhUaPu0iTMFiO0C8dQWHiD1UnB5CDzWk-LgY5gkivsthgrzD0y7ZXV3QKeScMp5chU0a944MtrocSg224ORxHRzuU0z5DXrlYSzu7WGeoJ9fPv9Yf2uub75erS-vGysUr43sWyZ64oEpoXtClW2BCSmBMk_0pqfAwUrQfsOBgpdAWuBKetIB80q1_ARd7L1382ZyvXWxZhjNIbxJEMy_lxgGc5t-GUplR3m3CM4OgpzuZ1eqmUKxbhwhujQXo_SSSndyAd__B27TnOPynGGESC65EAt0vodsTqVk5_8kocQ8dme-r6-eulvQd38nfwYPZfHfLLCWXw</recordid><startdate>200507</startdate><enddate>200507</enddate><creator>Ukropcova, Barbara</creator><creator>McNeil, Michele</creator><creator>Sereda, Olga</creator><creator>de Jonge, Lilian</creator><creator>Xie, Hui</creator><creator>Bray, George A</creator><creator>Smith, Steven R</creator><general>American Society for Clinical Investigation</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>S0X</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200507</creationdate><title>Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor</title><author>Ukropcova, Barbara ; McNeil, Michele ; Sereda, Olga ; de Jonge, Lilian ; Xie, Hui ; Bray, George A ; Smith, Steven R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-5d724d0fa2648d016c7a2455a12f08bd1a3ac5a8fb3a1af5a07a365f09a2f6673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biomedical research</topic><topic>Dietary Fats - metabolism</topic><topic>Glucose - metabolism</topic><topic>Glucose Clamp Technique</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Insulin Resistance</topic><topic>Male</topic><topic>Models, Biological</topic><topic>Muscle Fibers, Skeletal - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Palmitic Acid - metabolism</topic><topic>Phenotype</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ukropcova, Barbara</creatorcontrib><creatorcontrib>McNeil, Michele</creatorcontrib><creatorcontrib>Sereda, Olga</creatorcontrib><creatorcontrib>de Jonge, Lilian</creatorcontrib><creatorcontrib>Xie, Hui</creatorcontrib><creatorcontrib>Bray, George A</creatorcontrib><creatorcontrib>Smith, Steven R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of clinical investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ukropcova, Barbara</au><au>McNeil, Michele</au><au>Sereda, Olga</au><au>de Jonge, Lilian</au><au>Xie, Hui</au><au>Bray, George A</au><au>Smith, Steven R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor</atitle><jtitle>The Journal of clinical investigation</jtitle><addtitle>J Clin Invest</addtitle><date>2005-07</date><risdate>2005</risdate><volume>115</volume><issue>7</issue><spage>1934</spage><epage>1941</epage><pages>1934-1941</pages><issn>0021-9738</issn><eissn>1558-8238</eissn><abstract>Metabolic flexibility of skeletal muscle, that is, the preference for fat oxidation (FOx) during fasting and for carbohydrate oxidation in response to insulin, is decreased during insulin resistance. The aim of this study was to test the hypothesis that the capacity of myotubes to oxidize fat in vitro reflects the donor's metabolic characteristics. Insulin sensitivity (IS) and metabolic flexibility of 16 healthy, young male subjects was determined by euglycemic hyperinsulinemic clamp. Muscle samples were obtained from vastus lateralis, cultured, and differentiated into myotubes. In human myotubes in vitro, we measured suppressibility (glucose suppression of FOx) and adaptability (an increase in FOx in the presence of high palmitate concentration). We termed these dynamic changes in FOx metabolic switching. In vivo, metabolic flexibility was positively correlated with IS and maximal oxygen uptake and inversely correlated with percent body fat. In vitro suppressibility was inversely correlated with IS and metabolic flexibility and positively correlated with body fat and fasting FFA levels. Adaptability was negatively associated with percent body fat and fasting insulin and positively correlated with IS and metabolic flexibility. The interindividual variability in metabolic phenotypes was preserved in human myotubes separated from their neuroendocrine environment, which supports the hypothesis that metabolic switching is an intrinsic property of skeletal muscle.</abstract><cop>United States</cop><pub>American Society for Clinical Investigation</pub><pmid>16007256</pmid><doi>10.1172/jci24332</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9738 |
| ispartof | The Journal of clinical investigation, 2005-07, Vol.115 (7), p.1934-1941 |
| issn | 0021-9738 1558-8238 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1159139 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adolescent Adult Biomedical research Dietary Fats - metabolism Glucose - metabolism Glucose Clamp Technique Humans In Vitro Techniques Insulin Resistance Male Models, Biological Muscle Fibers, Skeletal - metabolism Oxidation-Reduction Palmitic Acid - metabolism Phenotype |
| title | Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T12%3A30%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dynamic%20changes%20in%20fat%20oxidation%20in%20human%20primary%20myocytes%20mirror%20metabolic%20characteristics%20of%20the%20donor&rft.jtitle=The%20Journal%20of%20clinical%20investigation&rft.au=Ukropcova,%20Barbara&rft.date=2005-07&rft.volume=115&rft.issue=7&rft.spage=1934&rft.epage=1941&rft.pages=1934-1941&rft.issn=0021-9738&rft.eissn=1558-8238&rft_id=info:doi/10.1172/jci24332&rft_dat=%3Cproquest_pubme%3E68016895%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=200535344&rft_id=info:pmid/16007256&rfr_iscdi=true |